Eco-friendly fabrication of silver nanoparticles from the red macroalga Scinaia moniliformis and evaluation of their biomedical activities

Hemangiben Sheth Charumati Jha   

Open Access   

Published:  Sep 29, 2025

DOI: 10.7324/JABB.2025.259214
Abstract

The present study reports the green synthesis of silver nanoparticles (AgNPs) using the red macroalga Scinaia moniliformis J. Agardh, an underexplored marine species rich in bioactive compounds. This eco-friendly method utilized the aqueous algal extract as a natural hydrophilic reducer and stabilizer. A visible color shift from light yellow to dark brown signified AgNP formation, which was further confirmed by characterization techniques including UV– Vis spectroscopy, FTIR, XRD, and TEM-SAED. A distinct surface plasmon resonance peak at 437 nm confirmed nanoparticle synthesis, while XRD validated their crystalline nature. FTIR indicated the presence of phenolics, proteins, and other biomolecules involved in reduction and capping. TEM analysis revealed predominantly spherical nanoparticles with an average size of 24.03 nm. Functionally, the AgNPs displayed strong antibacterial activity against both Gram-positive and Gram-negative bacteria, with the highest inhibition against Escherichia coli and Shigella dysenteriae. They also exhibited potent, dose-dependent antioxidant activity (IC?? = 41.09 ± 0.35 μg) and significant cytotoxicity against MCF-7 breast cancer cells (IC?? = 51.49 ± 0.45 μg). These findings underscore the novelty of using S. moniliformis for nanoparticle synthesis and highlight the broad biomedical applicability of the resulting AgNPs in antimicrobial and anticancer therapeutics.


Keyword:     Red macroalga Silver nanoparticles Antioxidant Antibacterial Anticancer


Citation:

Sheth H, Jha C. Eco-friendly fabrication of silver nanoparticles from the red macroalga Scinaia moniliformis and evaluation of their biomedical activities. J Appl Biol Biotech 2025. Article in Press. http://doi.org/10.7324/JABB.2025.259214

Copyright: Author(s). This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.

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Foram Patel, Khushali Upadhyay, Denni Mammen, Elizabeth Robin, A.V. Ramachandran, Darshee Baxi

Bioactive properties of the extracts of peels, pomace, seeds, and essential oils of Citrus limon and Citrus aurantifolia

Folasade Oluwatobi, Olakunle Afolabi, Pius Okiki, Funmilayo Adeniyi, Oghenerobor Akpor

High resolution-liquid chromatograph mass spectrometer characterization of bioactive compounds in pineapple wastes: Valorization of antioxidant and enzymatic activity

Suman Polaki, Sourav Nayak, K. Sampad Kumar, Rabi Prasad B

Recent important insight into nutraceuticals potential of pigmented rice cultivars: A promising ingredient for future food

Le Thi Kim Loan, Bui The Vinh, Ngo Van Tai

Elucidation of antioxidant compounds recovery capacity from “Cam” purple rice bran by different sustainable extraction techniques

Le Thi Kim Loan, Bui The Vinh, Ngo Van Tai

Secondary metabolite profiles, antimicrobial and antioxidant activities of callus, and leaves extract of Piper sarmentosum Roxb.

Junairiah Junairiah, Listijani Suhargo, Tri Nurhariyati, Nabilah Istighfari Zuraidassanaaz

Assessment of in vitro antioxidant properties and anticancer potential of Cucumis pubescens Willd. a medicinal fruit, utilizing human lung cancer cell line (A549)

T. Sundari, R. Kavitha, B. Mythili Gnanamangai, S. Saranya

Exploring Bougainvillea glabra flowers: a promising source of natural antimicrobial and anticancer agents

Wanchat Sirisarn, Auemphon Mordmuang, Kankamol Kerdkumthong, Sompop Saeheng,,

The effectiveness of the use of antioxidant formulations in the storage of fat from the Pacific sardines Sardinops melanostictus

Oksana V. Tabakaeva, Lidia V. Shulgina,, Mouhamad Alrajab, Anton V. Tabakaev, Pavel A. Shinkaruk, Varvara D. Stepochkina

Evaluation and characterization of endophytic bacteria from Capparis decidua (Forssk.) Edgew. for their antifungal and antioxidant activities

Sudesh Kumari, Prity Gulia, Pooja Choudhary, Ritu Pasrija, Mehak Dangi, Anil Kumar Chhillar

Sustainable improvement of nutrition quality and biological activity from cassava residue and okara through solid-state fermentation by Pleurotus citrinopileatus mycelium

Hang Nguyen Thi Bich, Cuong Chi Doan, Uyen Nguyen Khanh Phan, Khanh Trang Vu Le, Thang Duc Bui, Munehiro Tanaka, Minh Van Vo

Green synthesised Catharanthus roseus-mediated iron oxide nanoparticles demonstrates enhanced antibacterial, antioxidant, and anti-diabetic properties

Priya Josson Akkara, Sonia Angeline Martin, Nathiya Thiagarajulu, Aanchal Bisht Bisht, Archana Mishal, Christina M. Mathew

Melatonin: Possible mechanism of commercial production by cyanobacteria for human welfare and sustainable agriculture

Sakshi Pandey, Shobhit Raj Vimal, Sheo Mohan Prasad

Selenium-enriched Bacillus siamensis and Staphylococcus arlettae isolated from fermented rice

Nandhini Govindaraj, Silambu Selvi Kumbamoorthy, Vishnupriya Subramaniyan, Sundaresan Sivapatham

Effects of triclosan at different salinity levels on swimming behavior, reproduction, and antioxidant status in the marine rotifer Brachionus plicatilis

Ojeshvi Ethiraj, Arjun Surya Subramanya, Abdul Raziq Raheem, Jaganathan Murugesan Kuppuswamy

Biostimulant effect of hydrolyzed Spirulina (Ficocyan®) on bioactive compounds in pigmented corn from Jalisco, Mexico

Raúl Armando Chavarín González, Valeria Yadira Ortiz Sosa, Ramón Rodríguez Macias, Ricardo Solis Zamora, Luis Alberto Anguiano Sevilla, Juan Carlos Pizano Andrade, Mario Alberto Ruiz López

Phytochemical profiling and in silico target exploration of hexadecanoic acid from Bergenia ciliata: An integrative approach combining docking, absorption, distribution, metabolism, and excretion, and biological activity studies

Sagar Vishwakarma, Vijeta Chaudhry, Sumit Chand, Soban Prakash, Kajal Singh, Ajay Singh, Rajendra Prasad, Harish Chandra

Microalgae: A valuable bio-resource for pharmaceuticals and nutraceuticals

Sanjesh Tiwari, Vandana Sharma, Anuradha Patel, Vaishali Yadav, Charu Kalra, Garima Singh, Namira Arif, Arushi Khandelwal, Anupam Tiwari, Dileep Kumar Singh

In vitro Antimicrobial Comparison of Taif and Egyptian Pomegranate Peels and Seeds Extracts

Ahmed Gaber , Mohamed M. Hassan , El-Dessoky S. Dessoky , Attia O. Attia

Potential use as a bio-preservative from lupin protein hydrolysate generated by alcalase in food system

Ali Osman, Ghada M. El-Araby, Hefnawy Taha

Antimicrobial Activity Screening of Marine Bacteria Isolated from the Machilipatnam Sea Coast of Andhra Pradesh, India

K. Bala Chandra, V. Umamaheswara Rao, Subhaswaraj Pattnaik, Siddhardha Busi

Antimicrobial effect of nanofluid including Zinc oxide (ZnO) nanoparticles and Mentha pulegium essential oil

Mona Jahanpanahi, Ali Mohamadi Sani

Inducible Antimicrobial Compounds (Halal) Production in Honey Bee Larvae (Apis mellifera) from Rumaida, Taif by injecting of various dead Microorganisms extracts

Abd-ElAziem Farouk, N. Thoufeek Ahamed, Othman AlZahrani, Akram Alghamdi, AbdulAziz Bahobail

Bioactive potential of Diadema sp. from the South East Coast of Mauritius

Lisa Karen Yee Chin Youne Ah Shee Tee, Daneshwar Puchooa, Vishwakalyan Bhoyroo

Application of Mentha suaveolens essential oil as an antimicrobial agent in fresh turkey sausages

Abdelaziz Ed-Dra, Fouzia Rhazi Filai, Mohamed Bou-Idra, Badr Zekkori, Aziz Bouymajane, Najia Moukrad, Faouzia Benhallam, Amar Bentayeb

Antibacterial activity of an endophytic fungus Lasiodiplodia pseudotheobromae IBRL OS-64 residing in leaves of a medicinal herb, Ocimum sanctum Linn.

Taufiq M.M.J., Darah I.

Antibacterial activity of leaf extract of Chromolaena odorata and the effect of its combination with some conventional antibiotics on Pseudomonas aeruginosa isolated from wounds

P. Odinakachukwu Omeke, J. Okechukwu Obi, N. A. Ibuchukwu Orabueze , Anthony Chibuogwu Ike

Response of green synthesized drug blended silver nanoparticles against periodontal disease triggering pathogenic microbiota

Neeraj Kumar Fuloria, Shivkanya Fuloria, Kok Yik Chia, Sundram Karupiah, Kathiresan Sathasivam

Characterization of extracellular polymeric substance producing isolates from wastewaters and their antibacterial prospective

Anita Rani Santal,Nater Pal Singh,Tapan Kumar Singha

Application of guava leaves extract on jelly candy to inhibit Streptococcus mutans

Yuniwaty Halim, Raphael Dimas Tri Nugroho, Hardoko,, Ratna Handayani

Identification and bioactivities of endophytic fungi from Lagenandra toxicaria Dalz. and Kaempferia rotunda L.

Praveen Krishnakumar, Mable Varghese, Maria Grace Joe, Asha Rajagopal, Leyon Varghese

Antibacterial activity and hormetic response of silver nanoparticles synthesized using leaflet extract of wheat (Triticum aestivum) and rice (Oryza sativa) crop plants

Vikas Pahal, Pankaj Kumar, Parveen Kumar, Vinod Kumar

In vitro evaluation of the antibacterial potential of flavonoid glycosides from Glinus oppositifolius (L.) Aug. DC.

K. Suresh Kannan, D. Kandavel, P. Rajalakshmi, P. Maheswari

Silver nanoparticles decorated natural products doped polyaniline hybrid materials for biomedical applications

K. Satish, K. Sumangala Bhat, Y. S. Ravikumar, M. N. K. Harish

Neutrophil gelatinase-associated lipocalin a proinflammatory polypeptide necessary for host cell survival in bacterial infection

Nichita Yadav Aare, Pawan Kumar Anoor, Swathi Raju M, N. Srinivas Naik, Sandeepta Burgula

Extraction of a novel bacteriocin from Lacticaseibacillus casei VITCM05 and its antibacterial activity against major food-borne pathogens

Jannatul Firdous Siddique, Mohanasrinivasan Vaithilingam

Chemical profiling, in vitro antibacterial, and cytotoxic properties of Elytranthe parasitica (L.) Danser – A hemiparasitic Indian mistletoe

Keragodu Paramesh Sharath, Raja Naika

The physicochemical and biological properties of novel silver nanoparticles synthesized by the extract of Holigarna ferruginea

Kumbar Mudakappa Manjunath, Y. L. Krishnamurthy

Chemical and antibacterial properties of chitosan derived from Mucor spp., Rhizopus. Oryzae and Hermetia illucens

Muhammad Yusuf Abduh,, Tri Ramadianti Shafitri,, Maryam Jamilah, Mochamad Firmansyah,, Robert Manurung

Production of antibacterial substance by immobilized cells of Geobacillus subterraneus Tm6Sp1 isolate of Mount Kamojang Crater, west java, against pathogenic bacteria

Candra Arumimaniyah, Ratu Safitri, Emma Rachmawati, Ani Melani Maskoen, Akeyla Tabina Tawangalun, Shinta Asarina

Green synthetic photo-irradiated chitin-silver nanoparticles for antimicrobial applications

Navya Kumari Tenkayala,, Laxman Vamshi Krishna Kandala, Roopkumar Sangubotla, Rambabu Gundla, Subramani Devaraju

Effects of abiotic and biotic stresses on antagonistic activities of symbiotic bacterial strains isolated from Adenosma bracteosum

Thanh-Dung Nguyen, Huu-Nghia Duong, Phu-Tho Nguyen,, Thuy-Trang Pham, Huu-Hiep Nguyen, Thi-Pha Nguyen, Huu-Thanh Nguyen,

Isolation and molecular analysis of bacilli producing bacteriocins with broad-spectrum antimicrobial potential from fermented milk and kefir

Namita Singh, Anand Narayan Singh, Minhaj Ahmad Khan