Research Article | Volume: 4, Issue: 4, July-August, 2016

Antimicrobial effect of nanofluid including Zinc oxide (ZnO) nanoparticles and Mentha pulegium essential oil

Mona Jahanpanahi Ali Mohamadi Sani   

Open Access   

Published:  Aug 26, 2016

DOI: 10.7324/JABB.2016.40410

This study was carried out to evaluate the in vitro antibacterial activity of nanofluid based on Mentha pulegium essential oil and Zinc oxide (ZnO) nanoparticles (NPs) against different bacterial species. The essential oil was obtained by hydro-distillation using Clevenger and then ZnO NPs were added at the rates 0, 300, 500 and 1000 ppm to prepare nanofluids. The agar disk diffusion and micro-dilution methods were used to study the antibacterial activity. Minimum inhibitory concentration (MIC) against B. cereus, S. aureus, S. entrica and E. coli was determined respectively 7.8, 3.9, 15.6 and 62.5 ppm for ZnO NPs. Minimum bactericidal concentration (MBC) against the mentioned bacteria was respectively 15.6, 7.8, 31.2 and 125 ppm for ZnO NPs. S. aureus and E. coli were respectively the most and the least sensitive species. ZnO nanoparticles improved the antibacterial activity of M. pulegium essential oil which shows the potent application of the particles in different industries like food packaging, food and pharmaceutical systems.

Keyword:     Nanofluid Mentha pulegium Antibacterial activity Zinc oxide Nanoparticles.


Mona Jahanpanahi, Ali Mohamadi Sani., Antimicrobial effect of nanofluid including Zinc oxide (ZnO) nanoparticles and Mentha pulegium essential oil. J App Biol Biotech. 2016; 4 (04): 085-089. DOI: 10.7324/JABB.2016.40410

Copyright: Author(s). This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.

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