Sustainable improvement of nutrition quality and biological activity from cassava residue and okara through solid-state fermentation by Pleurotus citrinopileatus mycelium
Vietnam's agri-food sector produces 1.64 million tons of byproducts yearly and contributes almost 26% of the country's GDP in 2023. A very small portion of this waste was turned into compost, with the majority being disposed of as waste in the environment. However, there has not been much done in the way of research or technical applications to utilize this residue up to this point. Hence, this study investigated the effect of solid-state fermentation with Pleurotus citrinopileatus mycelia on polysaccharide (PS) and protein contents, antioxidant properties, probiotic growth stimulation, pathogenic inhibition, and bio-physicochemical properties of extracts from culture medium before and after fermentation was investigated. The findings indicated that when cassava (CASS) residue and okara are mixed in a 1:1 ratio, the mycelium develops swiftly uniform, even white, very thick, and high density. The total protein, ash, and PS contents from this fermented mixture were raised by 65.12%, 70%, and 57.24%, respectively. The PS extract inhibited the growth of Escherichia coli and Staphylococcus aureus with sterile ring diameters of 3.47 ± 0.38 and 3.06 ± 0.27 cm, respectively; stimulated the growth of Lactiplantibacillus plantarum with a colony density of 9.34 logCFU/ml after 24 hours of culture; and increased antioxidant capacity with IC50 = 3,287.62 g/ml. Heavy metals content, bacteria, yeasts, and mold levels were all lower than the allowable thresholds as recommended for animal feed purposes. The results show that P. citrinopileatus mycelium can ferment CASS residue and okara to produce a safe and nutritious source of animal feed supplements. This offers a viable approach for enhancing the added value of agricultural residue. Thus, more research is required to assess the financial viability of using fermented substrates produced by oyster mycelium as an additional feed source for animals. Researchers can also concentrate on conducting additional studies on the safety, use, and biological activity of the isolated PS fractions in the functional food sector.
Bich HNT, Doan CC, Phan UNK, Lê KTV, Bui TD, Tanaka M, Vo MV. Sustainable improvement of nutrition quality and biological activity from cassava residue and okara through solid-state fermentation by Pleurotus citrinopileatus mycelium. J Appl Biol Biotech. 2024. http://doi.org/10.7324/JABB.2024.204855
1. General Statistic Office of Vietnam. Statistical yearbook of Vietnam. General Statistic Office of Vietnam, Hanoi, Vietnam, 2023. | |
2. Bich TN, Lam NT, Thuan NK, Trung LQ, Khanh NP, Dung NM, et al. Livestock situation and common diseases in cows in Ben Tre province. Vet Sci Technol 2021;3:51-7. | |
3. Toan DT, Van Luu N. Situation of antibiotic use in raising pigs and chickens in some farms in Bac Giang province. Vietnam J Agric Sci 2015;13:717-22. | |
4. Valverde ME, Hernández-Pérez T, Paredes-López O. Edible mushrooms: improving human health and promoting quality life. Int J Microbiol 2015;2015:376387. https://doi.org/10.1155/2015/376387 | |
5. Elhusseiny SM, El-Mahdy TS, Elleboudy NS, Farag MMS, Aboshanab KM, Yassien MA. Immunomodulatory activity of extracts from five edible basidiomycetes mushrooms in Wistar albino rats. Sci Rep 2022;12:12423. https://doi.org/10.1038/s41598-022-16349-2 | |
6. Zhao S, Gao Q, Rong C, Wang S, Zhao Z, Liu Y, et al. Immunomodulatory effects of edible and medicinal mushrooms and their bioactive immunoregulatory products. J Fungi (Basel) 2020;6(4):269. https://doi.org/10.3390/jof6040269 | |
7. Krittanawong C, Isath A, Hahn J, Wang Z, Fogg SE, Bandyopadhyay D, et al. Mushroom consumption and cardiovascular health: a systematic review. Am J Med 2021;134(5):637-42. https://doi.org/10.1016/j.amjmed.2020.10.035 | |
8. Rauf A, Joshi PB, Ahmad Z, Hemeg HA, Olatunde A, Naz S, et al. Edible mushrooms as potential functional foods in amelioration of hypertension. Phytother Res 2023;37(6):2644-60. https://doi.org/10.1002/ptr.7865 | |
9. Li M, Yu L, Zhao J, Zhang H, Chen W, Zhai Q, et al. Role of dietary edible mushrooms in the modulation of gut microbiota. J Funct Foods 2021;83:104538. https://doi.org/10.1016/j.jff.2021.104538 | |
10. Jayachandran M, Xiao J, Xu B. A critical review on health promoting benefits of edible mushrooms through gut microbiota. Int J Mol Sci 2017;18(9):2-12. https://doi.org/10.3390/ijms18091934 | |
11. Gariboldi MB, Marras E, Ferrario N, Vivona V, Prini P, Vignati F, et al. Anti-cancer potential of edible/medicinal mushrooms in breast cancer. Int J Mol Sci 2023;24(12):1-30. https://doi.org/10.3390/ijms241210120 | |
12. Nowakowski P, Markiewicz-?ukowska R, Bielecka J, Mielcarek K, Grabia M, Socha K. Treasures from the forest: evaluation of mushroom extracts as anti-cancer agents. Biomed Pharmacother 2021;143:112106. https://doi.org/10.1016/j.biopha.2021.112106 | |
13. Liuzzi GM, Petraglia T, Latronico T, Crescenzi A, Rossano R. Antioxidant compounds from edible mushrooms as potential candidates for treating age-related neurodegenerative diseases. Nutrients 2023;15:1-23. https://doi.org/10.3390/nu15081913 | |
14. Mwangi RW, Macharia JM, Wagara IN, Bence RL. The antioxidant potential of different edible and medicinal mushrooms. Biomed Pharmacother 2022;147:112621. https://doi.org/10.1016/j.biopha.2022.112621 | |
15. Shamim MZ, Mishra AK, Kausar T, Mahanta S, Sarma B, Kumar V, et al. Exploring edible mushrooms for diabetes: unveiling their role in prevention and treatment. Molecules 2023;28:2-25. https://doi.org/10.3390/molecules28062837 | |
16. Dubey SK, Chaturvedi VK, Mishra D, Bajpeyee A, Tiwari A, Singh MP. Role of edible mushroom as a potent therapeutics for the diabetes and obesity. 3 Biotech 2019;9:450. https://doi.org/10.1007/s13205-019-1982-3 | |
17. Yu Y, Liu Z, Song K, Li L, Chen M. Medicinal value of edible mushroom polysaccharides: a review. J Future Foods 2023;3:16-23. https://doi.org/10.1016/j.jfutfo.2022.09.003 | |
18. Heidari F, Øverland M, Hansen JØ, Mydland LT, Urriola PE, Chen C, et al. Solid-state fermentation of Pleurotus ostreatus to improve the nutritional profile of mechanically-fractionated canola meal. Biochem Eng J 2022;187:108591. https://doi.org/10.1016/j.bej.2022.108591 | |
19. Díaz-Godínez G, Téllez-Téllez M, Sánchez C, Díaz R. Characterization of the solid-state and liquid fermentation for the production of laccases of Pleurotus ostreatus. In: Jozala AF (ed.). Fermentation processes, IntechOpen, Rijeka, Croatia, pp 57-74, 2017. https://doi.org/10.5772/64239 | |
20. Yin Z, Sun-Waterhouse D, Wang J, Ma C, Waterhouse GIN, Kang W. Polysaccharides from edible fungi Pleurotus spp.: advances and perspectives. J Future Foods 2021;1:128-40. https://doi.org/10.1016/j.jfutfo.2022.01.002 | |
21. Sharma A, Sharma A, Tripathi A. Biological activities of Pleurotus spp. polysaccharides: a review. J Food Biochem 2021;45:e13748. https://doi.org/10.1111/jfbc.13748 | |
22. Cao XY, Liu JL, Yang W, Hou X, Li QJ. Antitumor activity of polysaccharide extracted from Pleurotus ostreatus mycelia against gastric cancer in vitro and in vivo. Mol Med Rep 2015;12:2383-9. https://doi.org/10.3892/mmr.2015.3648 | |
23. Khinsar KH, Abdul S, Hussain A, Ud Din R, Lei L, Cao J, et al. Anti-tumor effect of polysaccharide from Pleurotus ostreatus on H22 mouse Hepatoma ascites in-vivo and hepatocellular carcinoma in-vitro model. AMB Express 2021;11:160. https://doi.org/10.1186/s13568-021-01314-5 | |
24. Owaid M, Alsaeedi S, Abed IA, Shahbazi P, Sabaratnam V. Antifungal activities of some Pleurotus species (Higher Basidiomycetes). Walailak J Sci Technol 2017;14:215-24. | |
25. Hearst R, Nelson D, McCollum G, Millar BC, Maeda Y, Goldsmith CE, et al. An examination of antibacterial and antifungal properties of constituents of Shiitake (Lentinula edodes) and Oyster (Pleurotus ostreatus) mushrooms. Complement Ther Clin Pract 2009;15:5-7. https://doi.org/10.1016/j.ctcp.2008.10.002 | |
26. Mishra V, Tomar S, Yadav P, Singh MP. Promising anticancer activity of polysaccharides and other macromolecules derived from oyster mushroom (Pleurotus sp.): an updated review. Int J Biol Macromol 2021;182:1628-37. https://doi.org/10.1016/j.ijbiomac.2021.05.102 | |
27. Stastny J, Marsik P, Tauchen J, Bozik M, Mascellani A, Havlik J, et al. Antioxidant and anti-inflammatory activity of five medicinal mushrooms of the genus Pleurotus. Antioxidants 2022;11:1569. https://doi.org/10.3390/antiox11081569 | |
28. dos Reis EE, Schenkel PC, Camassola M. Effects of bioactive compounds from Pleurotus mushrooms on COVID-19 risk factors associated with the cardiovascular system. J Integr Med 2022;20:385- 95. https://doi.org/10.1016/j.joim.2022.07.002 | |
29. Vetvicka V, Gover O, Karpovsky M, Hayby H, Danay O, Ezov N, et al. Immune-modulating activities of glucans extracted from Pleurotus ostreatus and Pleurotus eryngii. J Funct Foods 2019;54:81-91. https://doi.org/10.1016/j.jff.2018.12.034 | |
30. Agunloye OM, Oboh G. Blood glucose lowering and effect of oyster (Pleurotus ostreatus) and shiitake (Lentinus subnudus) supplemented diet on key enzymes linked diabetes and hypertension in streptozotocin-induced diabetic in rats. Food Front 2022;3:161- 71. https://doi.org/10.1002/fft2.111 | |
31. Hao Y, Sun H, Zhang X, Wu L, Zhu Z. A novel polysaccharide from Pleurotus citrinopileatus mycelia: structural characterization, hypoglycemic activity and mechanism. Food Biosci 2020;37:100735. https://doi.org/10.1016/j.fbio.2020.100735 | |
32. Li YR, Liu QH, Wang HX, Ng TB. A novel lectin with potent antitumor, mitogenic and HIV-1 reverse transcriptase inhibitory activities from the edible mushroom Pleurotus citrinopileatus. Biochim Biophys Acta 2008;1780:51-7. https://doi.org/10.1016/j.bbagen.2007.09.004 | |
33. Wang JC, Hu SH, Liang ZC, Yeh CJ. Optimization for the production of water-soluble polysaccharide from Pleurotus citrinopileatus in submerged culture and its antitumor effect. Appl Microbiol Biotechnol 2005;67:759-66. https://doi.org/10.1007/s00253-004-1833-x | |
34. Zhang J, Wang G, Li H, Zhuang C, Mizuno T, Ito H, et al. Antitumor polysaccharides from a Chinese mushroom, "yuhuangmo," the fruiting body of Pleurotus citrinopileatus. Biosci Biotechnol Biochem 1994;58:1195-201. https://doi.org/10.1271/bbb.58.1195 | |
35. Lee YL, Huang GW, Liang ZC, Mau JL. Antioxidant properties of three extracts from Pleurotus citrinopileatus. LWT Food Sci Technol 2007;40:823-33. https://doi.org/10.1016/j.lwt.2006.04.002 | |
36. Liu X, Pang H, Gao Z, Zhao H, Zhang J, Jia L. Antioxidant and hepatoprotective activities of residue polysaccharides by Pleurotus citrinopileatus. Int J Biol Macromol 2019;131:315-22. https://doi.org/10.1016/j.ijbiomac.2019.03.074 | |
7. Huang Y, Gao Y, Pi X, Zhao S, Liu W. In vitro hepatoprotective and human gut microbiota modulation of polysaccharide-peptides in Pleurotus citrinopileatus. Front Cell Infect Microbiol 2022;12:892049. https://doi.org/10.3389/fcimb.2022.892049 | |
38. Minato KI, Laan L, van Die I, Mizuno M. Pleurotus citrinopileatus polysaccharide stimulates anti-inflammatory properties during monocyte-to-macrophage differentiation. Int J Biol Macromol 2018;122:705-12. https://doi.org/10.1016/j.ijbiomac.2018.10.157 | |
39. Sheng Y, Zhao C, Zheng S, Mei X, Huang K, Wang G, et al. Anti-obesity and hypolipidemic effect of water extract from Pleurotus citrinopileatus in C57BL/6J mice. Food Sci Nutr 2019;7(4):1295- 301. https://doi.org/10.1002/fsn3.962 | |
40. Meng M, Sun Y, Bai Y, Xu J, Sun J, Han L, et al. A polysaccharide from Pleurotus citrinopileatus mycelia enhances the immune response in cyclophosphamide-induced immunosuppressed mice via p62/Keap1/Nrf2 signal transduction pathway. Int J Biol Macromol 2023;228:165-77. https://doi.org/10.1016/j.ijbiomac.2022.12.142 | |
41. Letti L, Vítola F, Pereira G, Karp S, Medeiros ABP, da Costa ESF, et al. Chapter 14 - Solid-state fermentation for the production of mushrooms. In: Pandey A, Larroche C, Soccol CR (eds.). Current developments in biotechnology and bioengineering, Elsevier, Amsterdam, The Netherlands, pp 285-318, 2018. https://doi.org/10.1016/B978-0-444-63990-5.00014-1 | |
42. Wang J, Jiang Q, Huang Z, Wang Y, Roubik H, Yang K, et al. Solid-state fermentation of soybean meal with edible mushroom mycelium to improve its nutritional, antioxidant capacities and physicochemical properties. Fermentation 2023;9(4):322. https://doi.org/10.3390/fermentation9040322 | |
43. Muliterno MM, Rodrigues D, de Lima FS, Ida EI, Kurozawa LE. Conversion/degradation of isoflavones and color alterations during the drying of okara. LWT 2017;75:512-19. https://doi.org/10.1016/j.lwt.2016.09.031 | |
44. Feng JY, Wang R, Thakur K, Ni ZJ, Zhu YY, Hu F, et al. Evolution of okara from waste to value added food ingredient: an account of its bio-valorization for improved nutritional and functional effects. Trends Food Sci Technol 2021;116:669-80. https://doi.org/10.1016/j.tifs.2021.08.011 | |
45. Maeda H, Nakamura A. 24 - Soluble soybean polysaccharide. In: Phillips GO, Williams PA (eds.). Handbook of hydrocolloids. Second edition, Woodhead Publishing, Sawston, UK, pp 693-709, 2009. https://doi.org/10.1533/9781845695873.693 | |
46. O'Toole DK. Soybean: soymilk, tofu, and okara. In: Wrigley C (ed.). Encyclopedia of grain science, Elsevier, Oxford, UK, pp 185-95, 2004 https://doi.org/10.1016/B0-12-765490-9/00173-7 | |
47. Liu K. 14 - Food use of whole soybeans. In: Johnson LA, White PJ, Galloway R (eds.). Soybeans, AOCS Press, Champaign, IL, pp 441-81, 2008. https://doi.org/10.1016/B978-1-893997-64-6.50017-2 | |
48. Li B, Qiao M, Lu F. Composition, nutrition, and utilization of okara (soybean residue). Food Rev Int 2012;28:231-52. https://doi.org/10.1080/87559129.2011.595023 | |
49. Suzuki A, Banna J. Improving diet quality for chronic disease prevention with okara "food waste." Am J Lifestyle Med 2021;15:14- 8. https://doi.org/10.1177/1559827620960099 | |
50. Turhan S, Temiz H, Sagir I. Utilization of wet okara in low-fat beef patties. J Muscle Foods 2007;18:226-35. https://doi.org/10.1111/j.1745-4573.2007.00081.x | |
51. Lv Y, Wang J, Xu L, Tang T, Su Y, Gu L, et al. Gel properties of okara dietary fiber-fortified soy protein isolate gel with/without NaCl. J Sci Food Agric 2023;103:411-19. https://doi.org/10.1002/jsfa.12155 | |
52. Matsumoto K, Watanabe Y, Yokoyama S. Okara, soybean residue, prevents obesity in a diet-induced murine obesity model. Biosci Biotechnol Biochem 2007;71:720-27. https://doi.org/10.1271/bbb.60563 | |
53. Rahman MM, Mat K, Ishigaki G, Akashi R. A review of okara (soybean curd residue) utilization as animal feed: nutritive value and animal performance aspects. Anim Sci J 2021;92:e13594. https://doi.org/10.1111/asj.13594 | |
54. Aro S. Improvement in the nutritive quality of cassava and its by-products through microbial fermentation. Afr J Biotechnol 2009;725:4789-97. | |
55. Cruz IA, Santos Andrade LR, Bharagava RN, Nadda AK, Bilal M, Figueiredo RT, et al. Valorization of cassava residues for biogas production in Brazil based on the circular economy: an updated and comprehensive review. Clean Eng Technol 2021;4:100196. https://doi.org/10.1016/j.clet.2021.100196 | |
56. Abouelezz K, Yuan J, Wang G, Bian G. The nutritive value of cassava starch extraction residue for growing ducks. Trop Anim Health Prod 2018;50:1231-38. https://doi.org/10.1007/s11250-018-1549-z | |
57. Morgan NK, Choct M. Cassava: nutrient composition and nutritive value in poultry diets. Anim Nutr 2016;2:253-61. https://doi.org/10.1016/j.aninu.2016.08.010 | |
58. Zheng Y, Xue S, Zhao Y, Li S. Effect of cassava residue substituting for crushed maize on in vitro ruminal fermentation characteristics of dairy cows at mid-lactation. Animals 2020;10(5):893. https://doi.org/10.3390/ani10050893 | |
59. Sabater C, Ruiz L, Delgado S, Ruas-Madiedo P, Margolles A. Valorization of vegetable food waste and by-products through fermentation processes. Front Microbiol 2020;11:581997. https://doi.org/10.3389/fmicb.2020.581997 | |
60. Oktaviani N, Sarwono KA, Utama GL. Bioconversion rice bran and cassava peel into yeasts cell walls mannoprotein as environmental friendly antioxidant. E3S Web Conf 2021;249:3004. https://doi.org/10.1051/e3sconf/202124903004 | |
61. Suriyapha C, Supapong C, So S, Wanapat M, Cherdthong A. Bioconversion of agro-industrial residues as a protein source supplementation for multiparous Holstein Thai crossbreed cows. PLoS One 2022;17:e0273916. https://doi.org/10.1371/journal.pone.0273916 | |
62. Verardi A, Sangiorgio P, Blasi A, Lopresto CG, Calabrò V. Bioconversion of crop residues using alternative fermentation-based approaches. Front Biosci 2023;15(3):17. https://doi.org/10.31083/j.fbe1503017 | |
63. Bala S, Garg D, Sridhar K, Inbaraj BS, Singh R, Kamma S, et al. Transformation of agro-waste into value-added bioproducts and bioactive compounds: micro/nano formulations and application in the agri-food-pharma sector. Bioengineering (Basel) 2023;10(2):152. https://doi.org/10.3390/bioengineering10020152 | |
64. Blasi A, Verardi A, Lopresto CG, Siciliano S, Sangiorgio P. Lignocellulosic agricultural waste valorization to obtain valuable products: an overview. Recycling 2023;8(4):61. https://doi.org/10.3390/recycling8040061 | |
65. Adnane I, Taoumi H, Elouahabi K, Lahrech K, Oulmekki A. Valorization of crop residues and animal wastes: anaerobic co-digestion technology. Heliyon 2024;10(5):e26440. https://doi.org/10.1016/j.heliyon.2024.e26440 | |
66. Lu X, Zhao Y, Li F, Liu P. Active polysaccharides from Lentinula edodes and Pleurotus ostreatus by addition of corn straw and xylosma sawdust through solid-state fermentation. Int J Biol Macromol 2023;228:647-58. https://doi.org/10.1016/j.ijbiomac.2022.12.264 | |
67. Liu Y, Sun Y, Huang G. Preparation and antioxidant activities of important traditional plant polysaccharides. Int J Biol Macromol 2018;111:780-86. https://doi.org/10.1016/j.ijbiomac.2018.01.086 | |
68. Siragusa S, Di Cagno R, Ercolini D, Minervini F, Gobbetti M, De Angelis M. Taxonomic structure and monitoring of the dominant population of lactic acid bacteria during wheat flour sourdough type I propagation using Lactobacillus sanfranciscensis starters. Appl Environ Microbiol 2009;75(4):1099-109. https://doi.org/10.1128/AEM.01524-08 | |
69. Rousseau V, Lepargneur JP, Roques C, Remaud-Simeon M, Paul F. Prebiotic effects of oligosaccharides on selected vaginal lactobacilli and pathogenic microorganisms. Anaerobe 2005;11(3):145-53. https://doi.org/10.1016/j.anaerobe.2004.12.002 | |
70. Adenipekun CO, Jonathan G. Nutritional requirements of Pleurotus florida (Mont.) Singer, a Nigerian mushroom. Pak J Nutr 2006;5(6):597-600. https://doi.org/10.3923/pjn.2006.597.600 | |
71. Maniyam MN, Sundarajoo A, Azman HH, Abdullah H, Yaacob NS. Rhodococcus strain UCC 0010 as green biocatalyst for enhanced biodecolourization of Congo red through response surface methodology. Int J Environ Sci Technol 2022;19:3305-22. https://doi.org/10.1007/s13762-021-03400-4 | |
72. Kumla J, Suwannarach N, Sujarit K, Penkhrue W, Kakumyan P, Jatuwong K, et al. Cultivation of mushrooms and their lignocellulolytic enzyme production through the utilization of agro-industrial waste. Molecules 2020;25(12):2811. https://doi.org/10.3390/molecules25122811 | |
73. Araújo NL, Avelino KV, Halabura MIW, Marim RA, Kassem AS, Linde GA, et al. Use of green light to improve the production of lignocellulose-decay enzymes by Pleurotus spp. in liquid cultivation. Enzyme Microb Technol 2021;149:109860. https://doi.org/10.1016/j.enzmictec.2021.109860 | |
74. Bakratsas G, Polydera A, Nilson O, Kossatz L, Xiros C, Katapodis P, et al. Single-cell protein production by Pleurotus ostreatus in submerged fermentation. Sustain Food Technol 2023;1(3):377-89. https://doi.org/10.1039/D2FB00058J | |
75. dos Santos Oliveira M, Feddern V, Kupski L, Cipolatti EP, Badiale- Furlong E, de Souza-Soares LA. Changes in lipid, fatty acids and | |
phospholipids composition of whole rice bran after solid-state fungal fermentation. Bioresour Technol 2011;102(17):8335-8. https://doi.org/10.1016/j.biortech.2011.06.025 | |
76. Abu OA, Tewe OO, Losel DM, Onifade AA. Changes in lipid, fatty acids and protein composition of sweet potato (Ipomoea batatas) after solid-state fungal fermentation. Bioresour Technol 2000;72(2):189- 92. https://doi.org/10.1016/S0960-8524(99)90102-5 | |
77. Yue F, Zhang J, Xu J, Niu T, Lü X, Liu M. Effects of monosaccharide composition on quantitative analysis of total sugar content by phenol-sulfuric acid method. Front Nutr 2022;9:963318. https://doi.org/10.3389/fnut.2022.963318 | |
78. Cano y Postigo LO, Jacobo-Velázquez DA, Guajardo-Flores D, Amezquita LE, García-Cayuela T. Solid-state fermentation for enhancing the nutraceutical content of agrifood by-products: recent advances and its industrial feasibility. Food Biosci 2021;41:100926. https://doi.org/10.1016/j.fbio.2021.100926 | |
79. Thomas L, Larroche C, Pandey A. Current developments in solid-state fermentation. Biochem Eng J 2013;81:146-61. https://doi.org/10.1016/j.bej.2013.10.013 | |
80. Musieba F, Okoth S, Mibey RK, Wanjiku S, Moraa K. Proximate composition, amino acids and vitamins profile of Pleurotus citrinopileatus Singer: an indigenous mushroom in Kenya. Am J Food Technol 2013;8(3):200-6. https://doi.org/10.3923/ajft.2013.200.206 | |
81. Gogoi P, Chutia P, Singh P, Mahanta CL. Effect of optimized ultrasound-assisted aqueous and ethanolic extraction of Pleurotus citrinopileatus mushroom on total phenol, flavonoids and antioxidant properties. J Food Process Eng 2019;42(6):e13172. https://doi.org/10.1111/jfpe.13172 | |
82. Mateos-Aparicio I, Mateos-Peinado C, Rupérez P. High hydrostatic pressure improves the functionality of dietary fibre in okara by-product from soybean. Innov Food Sci Emerg Technol 2010;11(3):445-50. https://doi.org/10.1016/j.ifset.2010.02.003 | |
83. Vong WC, Liu SQ. Biovalorisation of okara (soybean residue) for food and nutrition. Trends Food Sci Technol 2016;52:139-47. https://doi.org/10.1016/j.tifs.2016.04.011 | |
84. Kechagia M, Basoulis D, Konstantopoulou S, Dimitriadi D, Gyftopoulou K, Skarmoutsou N, et al. Health benefits of probiotics: a review. ISRN Nutr 2013;2013:481651. https://doi.org/10.5402/2013/481651 | |
85. Fuller R. Probiotics in man and animals. J Appl Bacteriol 1989;66(5):365-78. https://doi.org/10.1111/j.1365-2672.1989.tb05105.x | |
86. Koutrotsios G, Patsou M, Mitsou EK, Bekiaris G, Kotsou M, Tarantilis PA, et al. Valorization of olive by-products as substrates for the cultivation of Ganoderma lucidum and Pleurotus ostreatus mushrooms with enhanced functional and prebiotic properties. Catalysts 2019;9(6):537. https://doi.org/10.3390/catal9060537 | |
87. Leksono BY, Cahyanto MN, Rahayu ES, Yanti R, Utami T. Enhancement of antioxidant activities in black soy milk through isoflavone aglycone production during indigenous lactic acid bacteria fermentation. Fermentation 2022;8(7):326. https://doi.org/10.3390/fermentation8070326 | |
88. Fleming D, Chahin L, Rumbaugh K. Glycoside hydrolases degrade polymicrobial bacterial biofilms in wounds. Antimicrob Agents Chemother 2017;61(2):e01998-16. https://doi.org/10.1128/AAC.01998-16 | |
89. Godoy MG, Amorim GM, Barreto MS, Freire D. Chapter 12 - Agricultural residues as animal feed: protein enrichment and detoxification using solid-state fermentation. In: Pandey A, Larroche C, Soccol CR (eds.). Current developments in biotechnology and bioengineering, Elsevier, Amsterdam, The Netherlands, pp 235-56, 2018. https://doi.org/10.1016/B978-0-444-63990-5.00012-8 |
Year
Month
Bioactivity assessment of endophytic fungi associated with Centella asiatica and Murraya koengii
Archana Nath, Jyoti Pathak and SR JoshiAsparagus racemosus extract increases the life span in Drosophila melanogaster
K. V. Kiran Kumar, K. S. Prasanna, J. S. AshadeviComparative three way analysis of biochemical responses in cereal and millet crops under salinity stress
Ritika Bhatt, Prem Prakash Asopa, Santosh Sihag, Rakesh Sharma, Sumita Kachhwaha, S.L. KothariImpact of Phyllanthus amarus extract on antioxidant enzymes in Drosophila melanogaster
N. Manasa, J. S. AshadeviAlterations in antioxidant defense system in hepatic and renal tissues of rats following aspartame intake
Saeed A. AlwaleediTen different brewing methods of green tea: comparative antioxidant study
Naila Safdar, Amina Sarfaraz, Zehra Kazmi, Azra YasminMedicinal and Functional Values of Thyme (Thymus vulgaris L.) Herb
Eqbal M. A. Dauqan, Aminah AbdullahBuffalo (Bubalus bubilis) colostrum and milk fat globule membrane fractions are potent antioxidants
N. Brijesha, H. S. AparnaEffect of extraction solvents on phenolic contents and antioxidant capacities of Artocarpus chaplasha and Carissa carandas fruits from Bangladesh
Gouri Dhar, Sonam Akther, Afrin Sultana, Uchen May, Mohammed Moinul Islam, Mowri Dhali, Dwaipayan SikdarIn Vitro Antidiabetic and Anti-oxidant Activities of Methanol Extract of Tinospora Sinensis
Anindita Banerjee, Bithin Maji, Sandip Mukherjee, Kausik Chaudhuri, Tapan SealChemical composition, antioxidant and antimicrobial activities of the essential oil of Vetiveria nigritana (Benth.) Stapf roots from Burkina Faso
Zenabou Semde, Jean Koudou, Cheikna Zongo, Gilles Figueredo, Marius K. Somda, Leguet Ganou, Alfred S. TraoreCrosstalk of brassinosteroids with other phytohormones under various abiotic stresses
Farhan Ahmad, Ananya Singh, Aisha KamalIn vitro propagation by axillary shoot proliferation, assessment of antioxidant activity, and genetic fidelity of micropropagated Paederia foetida L.
Biswaranjan Behera, Priyajeet Sinha, Sushanto Gouda, Sakti K. Rath, Durga P. Barik, Padan K. Jena, Pratap C. Panda, Soumendra K. NaikEffect of growth hormones in induction of callus, antioxidants, and antibacterial activity in Nerium odorum
Avinash Prakasha, S UmeshaAroclor 1254 induced oxidative stress and histopathological changes in mice liver
Jalpa Raja, Shweta Pathak, Rahul KunduA study of endophytic fungi Neofusicoccum ribis from Gandaria (Bouea macrophylla Griffith) as enzyme inhibitor, antibacterial, and antioxidant
Trisanti Anindyawati, PraptiwiDetermination of phytochemical, antioxidant, antimicrobial, and protein binding qualities of hydroethanolic extract of Celastrus paniculatus
Vijay Kumar¥, Simranjeet Singh¥, Arjun Singh¥, Amit Kumar Dixit¥, Bhavana Shrivastava, Sapna Avinash Kondalkar, Joginder Singh, Ravindra Singh, Gurpreet Kaur Sidhu, Rajesh Partap Singh, Varanasi Subhose, Om PrakashEffect of Trema guineensis leaves (celtidaceae) on glucose-induced hypertension in Wistar rats
Balakiyém Kadissoli, P A Mouzou, T Pakoussi, K Eklu-Gadegbeku, A K Aklikokou, M GbeassorPhytochemical analysis, antimicrobial and antioxidant activities of Aidia borneensis leaf extracts
Zulhamizan Awang-Jamil, Aida Maryam Basri, Norhayati Ahmad, Hussein TahaComparative chemical and biological investigations of three Saudi Astragalus species
Mohamed A. AshourPhytochemical analysis and antioxidant potential of Ocimum gratissimum Linn (Lamiaceae) commonly consumed in the Republic of Benin
Hinnoutondji Wilfrid Kpètèhoto, Abdou Madjid Olatoundé Amoussa, Roch Christian Johnson, Eustache Enock Meinsan Houéto , Franck Maurille Zinsou Mignanwandé, Hounnankpon Yédomonhan, Frédéric Loko, Honoré Bankolé, Latifou LagnikaPleurotus pulmonarius (Fr.) Quel. (Pleurotaceae): In vitro antioxidant evaluation and the isolation of a steroidal isoprenoid
Blessing Onyinye Okonkwo, Ozadheoghene Eriarie Afieroho, Emeka Daniel Ahanonu, Lambert Okwubie, Kio Anthony AboStudy of the changes in the growth, protein, and bioactive profile of Chlorella emersonii KJ725233 in response to sodium and ammonium nitrate
Sneha Sunil Sawant, Varsha Kelkar-ManeDetermination of phenolic content and antioxidant capacity of Launaea resedifolia from Algerian Sahara
Amina Bouguerra, Mohamed Hadjadj, Mesaouda Dekmouche, Zhour Rahmani, Houssine DendouguiInvestigation of morphological, phytochemical, and enzymatic characteristics of Anethum graveolens L. using selenium in combination with humic acid and fulvic acid
Parviz Samavatipour, Vahid Abdossi, Reza Salehi, Saeed Samavat,Alireza Ladan MoghadamA study on the salinity stress effects on the biochemical traits of seedlings and its relationship with resistance toward sensitive and tolerant flax genotypes
Yousef Alaei, Seyed Kamal Kazemitabar, Mohammad Zaefi Zadeh, Hamid Najafi Zarini, Gaffar KianiNyctanthes arbor-tristis: Comprehensive review on its pharmacological, antioxidant, and anticancer activities
Smita Parekh, Anjali SoniAnti-quorum sensing, antibacterial, antioxidant activities, and phytoconstituents analysis of medicinal plants used in Benin: Acacia macrostachya (Rchb. ex DC.)
Mounirou Tchatchedre, Abdou Madjid O. Amoussa, Ménonvè Atindehou, Aminata P. Nacoulma, Ambaliou Sanni, Martin kiendrebeogo, Latifou LagnikaStudies on the mechanism of desiccation tolerance in the resurrection fern Adiantum raddianum
Tumkur Govindaraju Banupriya, Chandraiah Ramyashree, Devaraja Akash, Neeragunda Shivaraj Yathish, Ramasandra Govindarao SharthchandraAntioxidant and antihyperlipidemic effects of aqueous seed extract of Daucus carota L. in triton ×100-induced hyperlipidemic mice
Habibu Tijjani, Abubakar Mohammed, Sani Muktar, Saminu Musa, Yusuf Abubakar, Adegbenro Peter Adegunloye, Ahmed Adebayo Ishola, Enoch Banbilbwa Joel, Carrol Domkat Luka, Adamu Jibril AlhassanRole of glutathione reductase and catalase enzyme in antioxidant defense mechanism in controlling fluoride-induced oxidative stress
Komal Sharma, Mamta Choudhary, Khushbu VermaBiological activities and phytochemicals of Hyptis capitata grown in East Kalimantan, Indonesia
Irawan Wijaya Kusuma, Rahmini, Enos Tangke Arung, Arif Yudo Pramono, Erwin, SupomoMycelial biomass, antioxidant, and myco-actives of mycelia of abalone mushroom Pleurotus cystidiosus in liquid culture
Kent Garcia, Chester Jhae Garcia, Reynante Bustillos, Rich Milton DulaySalt stress, its impacts on plants and the strategies plants are employing against it: A review
Zeenat Mushtaq, Shahla Faizan, Basit GulzarImpact of oxidizing, reducing, and stabilizing agents on the inhibitory properties of Cyamopsis tetragonoloba trypsin inhibitor
Preeti Patidar, Mahima Golani, Sumati Hajela, Krishnan HajelaIdentification of highest L-Methioninase enzyme producers among soil microbial isolates, with potential antioxidant and anticancer properties
D. Kavya, Varalakshmi Kilingar NadumaneAstaxanthin: An algae-based natural compound with a potential role in human health-promoting effect: An updated comprehensive review
Jinu Medhi, Mohan Chandra KalitaLinalool protects hippocampal CA1 neurons and improves functional outcomes following experimental ischemia/reperfusion in rats
Vishal Airao, Prakruti Buch, Tejas Sharma, Devendra Vaishnav, Sachin ParmarModification of the time of incubation in colorimetric method for accurate determination of the total antioxidants capacity using 2,2-diphenyl-1-picrylhydrazyl stable free radical
Abhipsa Bal, Samar Gourav Pati, Falguni Panda, Biswaranjan PaitalHR-LC-MS based profiling of phytochemicals from methanol extracts of leaves and bark of Myristica dactyloides Gaertn. from Western Ghats of Karnataka, India
Kuppuru Mallikarjunaiah Marulasiddaswamy, Bettadapura Rameshgowda Nuthan,, Channarayapatna-Ramesh Sunilkumar, Shrisha Naik Bajpe,, Kigga Kaadappa Sampath Kumara, Shailasree Sekhar, Kukkundoor Ramachandra KiniPreclinical evaluation of anticataract activity of Mentha spicata leaves on isolated goat lens by an in vitro model
Shreya Mohandas, Saahiba Thaleshwari, Myrene Roselyn DsouzaGreen synthesis, characterizations, and in vitro biological evaluation of Cu (II) complexes of quercetin with N ^ N ligands
Tanu Srivastava, Sunil Kumar Mishra, Om Prakash TiwariOptimization of extraction conditions of phytochemical compounds in “Xiem” banana peel powder using response surface methodology
Ngo Van Tai, Mai Nhat Linh, Nguyen Minh ThuyAntioxidative, antiproliferative, and apoptosis effect of Coleus tuberosus flesh and peel ethanol extracts on cervical cancer cell lines
Mutiara Nugraheni, Windarwati Windarwati, Badraningsih LastariwatiComparative study of hydroalcoholic extracts of Bryophyllum pinnatum and Macrotyloma uniflorum for their antioxidant, antiurolithiatic, and wound healing potential
Chetna Faujdar, PriyadarshiniQualitative and quantitative analysis of Precocene II, estimation of enzymatic, nonenzymatic antioxidant, and cytotoxic potentials of methyl jasmonate-elicited shoot culture of Ageratum conyzoides Linn.
Selvaraj Vasantharani, Ramaraj Thirugnanasampandan, Gunasekaran BhuvaneswariSuppression of the RAGE gene expression in RAW 264.7 murine leukemia cell line by ethyl acetate extract of Mikania micrantha (L.) Kunth.
Alex Zohmachhuana, Malsawmdawngliana Tlaisun, Vabeiryureilai Mathipi, Lalrinzuali Khawlhring, Joyce Sudandara PriyaA review on fish peptides isolated from fish waste with their potent bioactivities
Ayusman Behera, Rajashree Das, Pranati Patnaik, Jyotirmaya Mohanty, Gargee MohantyQuantification of phytochemicals and in vitro antioxidant activities from various parts of Euphorbia neriifolia Linn.
Priya Chaudhary, Pracheta JanmedaInfluence of soaking and germination treatments on the nutritional, anti-nutritional, and bioactive composition of pigeon pea (Cajanus cajan L.)
Qurat Ul Eain Hyder Rizvi, Krishan Kumar, Naseer Ahmed, Ajar Nath Yadav, Divya Chauhan, Priyanka Thakur, Sumaira Jan, Imran SheikhImpact of diverse processing treatments on nutritional and anti-nutritional characteristics of soybean (Glycine max L.)
Priyanka Thakur, Krishan Kumar, Naseer Ahmed, Ajar Nath Yadav, Sunil Kumar, Qurat Ul Eain Hyder Rizvi, Divya Chauhan, Sumaira JanCathelicidin-HR from Hoplobatrachus rugulosus: an antioxidant peptide that performs a protective effect against UV/H2O2 -induced DNA damage
Piyachat Wiriyaampaiwong, Chutima Karnmongkol, Arpaporn Punpad, Nattapong Srisamoot, Wutti Rattanavichai, Alongkod Tanomtong, Sakda Daduang,, Sompong Klaynongsruang,, Anupong Tankrathok,Woodfordia fruticosa (Linn.) Kurz’s fungal endophyte Mucor souzae’s secondary metabolites, kaempferol and quercetin, bestow biological activities
Kavyashree Doreswamy, Priyanka Shenoy, Sneha Bhaskar, Ramachandra K. Kini, Shailasree SekharCharacterization of the crude extract of Portulaca oleracea and the determination of the polyphenol oxidase kinetics in the presence of Cu and Zn
Omar Mohammad Atrooz, Shada Zaher Al-MaitahEffect of diverse fermentation treatments on nutritional composition, bioactive components, and anti-nutritional factors of finger millet (Eleusine coracana L.)
Sumaira Jan, Krishan Kumar, Ajar Nath Yadav, Naseer Ahmed, Priyanka Thakur, Divya Chauhan, Qurat-Ul-Eain Hyder Rizvi, Harcharan Singh DhaliwalOptimization of active antioxidative defatted Canarium indicum L. (Canary) protein hydrolysate production
Cintya Nurul Apsari,, Ilma Nugrahani, Sukrasno, Tutus GusdinarElemental, nutritional, and phytochemical profiling and antioxidant activity of Cordia obliqua Willd. (Clammy Cherry): An important underutilized forest tree of East India
Mamta Naik#,, Shashikanta Behera#,,, Sadhni Induar, Swaraj K. Babu, Pradeep K. NaikEffects of enzymatic hydrolysis on the antioxidant activity of protein hydrolysate derived from the larvae of black soldier fly (Hermetia illucens L.)
Muhammad Yusuf Abduh,, Diah Ayu Prawitasari,, Ula Aulia Fitrian,, Mochamad Firmansyah,Evaluation of functional characteristics of roselle seed and its use as a partial replacement of wheat flour in soft bread making
Nguyen Minh Thuy, Nguyen Bao Tram, Dinh Gia Cuong, Huynh Khanh Duy, Ly Thanh An, Vo Quoc Tien, Tran Ngoc Giau, Ngo Van TaiTotal phenolic, flavonoid contents, and antioxidant activity of three selected Portulaca grandiflora mutants in MV8 generation as a result of recurrent irradiation technique
Waras Nurcholis,, Syarifah Iis Aisyah, Regina Agritena Mayrischa Saraswati, Yoshua Shandy YudhaInsights into the impact of spermidine in reducing salinity stress in Gerbera jamesonii
Javeria Uzma, Sai Krishna Talla, Praveen MamidalaIn vitro antioxidant and antibacterial potential of biosynthesized yttrium oxide nanoparticles using floral extract of Illicium verum
Karthikeyan Kandasamy, Premkumar KumpatiEffect of combined NPK fertilizer on polyphenol contents and antioxidant activity in methanol extract of Curcuma xanthorhiza
Minarni Minarni, Rayandra Asyhar, Amira Amandanisa, Sintya Ainun, Yoshua Shandy Yudha, I Made Artika,, Waras Nurcholis,Dehydration kinetics of green banana slices, characterization of optimized product based on physicochemical, nutritional, optical, and sensory attributes
Ram Kaduji Gadhave, Ravneet Kaur, Rahul Das, Kamlesh PrasadAssessment of morpho-agronomic and yield attributes in gamma-irradiated mutants of Kalanamak rice (Oryza sativa L.)
Tanmai Mishra, Anjali Singh, Virendra Kumar Madhukar, Ashutosh Kumar Verma, Shambhavi Mishra, Rajveer Singh ChauhanMetabolic profile, bioactivities, and variations in chemical constituents of essential oils of twenty mango ginger (Curcuma amada) accessions
Jyotirmayee Lenka, Snehalata Khuntia, Basudeba Kar, Suprava SahooPhenolic compounds and in vitro antioxidant activity of spray-dried and freeze-dried aqueous extracts of sea cucumber (Holothuria tubulosa)
Fadna Aatab, Fatima Bellali, Fatima Zahra Aboudamia, Ahmed Errhif, Mariem KharroubiOptimization of pasteurization process of the ready-to-drink beverage from Hong Quan (Flacourtia jangomas) fruit by response surface methodology
Tan Duy Nguyen,, Tuyen Thi Xuan Vo,, Khang Nghia Tran,Increasing polyphenol antioxidant in Orthosiphon stamineus Benth leaves with fermentation extraction by Saccharomyces cerevisiae ATCC-9763
Muhammad Aria Chandra, Khaswar Syamsu, Laksmi Ambarsari, Nurul Fatimah, Waras Nurcholis,Phytochemical composition and antiproliferative activity of Opuntia elatior Mill.: In vitro and in silico studies on breast cancer cell line MCF-7
Foram Patel, Khushali Upadhyay, Denni Mammen, Elizabeth Robin, A.V. Ramachandran, Darshee BaxiBioactive properties of the extracts of peels, pomace, seeds, and essential oils of Citrus limon and Citrus aurantifolia
Folasade Oluwatobi, Olakunle Afolabi, Pius Okiki, Funmilayo Adeniyi, Oghenerobor AkporHigh resolution-liquid chromatograph mass spectrometer characterization of bioactive compounds in pineapple wastes: Valorization of antioxidant and enzymatic activity
Suman Polaki, Sourav Nayak, K. Sampad Kumar, Rabi Prasad BRecent important insight into nutraceuticals potential of pigmented rice cultivars: A promising ingredient for future food
Le Thi Kim Loan, Bui The Vinh, Ngo Van TaiSolid-state fermentation of pigment producing endophytic fungus Fusarium solani from Madiwala lake and its toxicity studies
Bhoomika Prakash Poornamath, Suma Sarojini, Saranya Jayaram, Soma Biswas, Anand Kaloor, Mridul UmeshElucidation of antioxidant compounds recovery capacity from “Cam” purple rice bran by different sustainable extraction techniques
Le Thi Kim Loan, Bui The Vinh, Ngo Van TaiSecondary metabolite profiles, antimicrobial and antioxidant activities of callus, and leaves extract of Piper sarmentosum Roxb.
Junairiah Junairiah, Listijani Suhargo, Tri Nurhariyati, Nabilah Istighfari ZuraidassanaazAssessment of in vitro antioxidant properties and anticancer potential of Cucumis pubescens Willd. a medicinal fruit, utilizing human lung cancer cell line (A549)
T. Sundari, R. Kavitha, B. Mythili Gnanamangai, S. SaranyaExploring Bougainvillea glabra flowers: a promising source of natural antimicrobial and anticancer agents
Wanchat Sirisarn, Auemphon Mordmuang, Kankamol Kerdkumthong, Sompop Saeheng,,The effectiveness of the use of antioxidant formulations in the storage of fat from the Pacific sardines Sardinops melanostictus
Oksana V. Tabakaeva, Lidia V. Shulgina,, Mouhamad Alrajab, Anton V. Tabakaev, Pavel A. Shinkaruk, Varvara D. StepochkinaEvaluation and characterization of endophytic bacteria from Capparis decidua (Forssk.) Edgew. for their antifungal and antioxidant activities
Sudesh Kumari, Prity Gulia, Pooja Choudhary, Ritu Pasrija, Mehak Dangi, Anil Kumar Chhillar