Research Article | Volume: 4, Issue: 3, May-June, 2016

Ten different brewing methods of green tea: comparative antioxidant study

Naila Safdar Amina Sarfaraz Zehra Kazmi Azra Yasmin   

Open Access   

Published:  Jun 21, 2016

DOI: 10.7324/JABB.2016.40306

In this study, three brands of green tea collected from different markets of Rawalpindi, Pakistan were screened to identify best brewing method for green tea preparation and characterize their antioxidant potential. Brand A, brand B and non-branded green tea were examined for antioxidant potential by DPPH (1,1-Diphenyl-2-picrylhydrazyl) radical scavenging assay and reducing power method. Samples were prepared by ten different recipes used in different cultures. Distilled water and ethanol were used as solvent. Three brewing methods with highest, lowest and moderate antioxidant activities were identified and analyzed for phytochemical constituents such as phenolic compounds, flavonoids, tannins, saponins, proteins, alkaloids and steroids. Cold cocktail showed highest antioxidant activity (85.9%), soft infusion showed moderate (70.6%) while hard infusion indicated lowest antioxidant potential (59.9%)amongst all the green tea brands tested. Phenolic compounds, tannins and proteins were found in all tea types with varying strength due to different brewing method. Alkaloids were not present in any type of green tea while steroids were found in brand A and B.

Keyword:     Camellia sinensis phytoconstituents antioxidant assay brewing methods Cold Cocktail.


Safdar N, Sarfaraz A, Kazmi Z, Yasmin A. Ten different brewing methods of green tea: comparative antioxidant study. J App Biol Biotech. 2016; 4 (03): 033-040. DOI: 10.7324/JABB.2016.40306

Copyright: Author(s). This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.

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