Plantago major synthesized silver nanoparticles in an anti-acne facial sheet mask: physical stability, safety, and efficacy

Christina Avanti Apriliana Muftilana Bella Fiesta Karina Citra Rani Johan Sukweenadhi Kartini Kartini Ricky Gonzali Mago Wahyu Vinovia Devi Devyani Diah Wulansari Aguslina Kirtishanti   

Open Access   

Published:  May 03, 2024

DOI: 10.7324/JABB.2024.169385

This study explores a novel approach to treat acne using silver nanoparticles (AgNPs) synthesized from Plantago major (Pm). We aimed to create a stable, safe, and effective anti-acne sheet mask. AgNPs were synthesized using water extracts of Pm leaves and formulated into an anti-acne sheet mask. The sheet mask was assessed for physical stability through freeze-thaw cycling tests and evaluated for safety through dermal irritation tests. The efficacy was assessed on the sheet mask’s impact on Propionibacterium acnes bacteria and inflammation. The anti-acne sheet mask containing AgNPs synthesized from Pm was found to be physically stable and safe for use, with no significant skin irritation observed. The formulation exhibited effective inhibition of P. acnes, particularly in the formula containing only AgNPs. In addition, the sheet mask preparations demonstrated substantial anti-inflammatory activity. The anti-acne sheet mask containing AgNPs synthesized from Pm exhibited favorable safety profile, inhibited P. acnes bacteria, and possessed anti-inflammatory properties.

Keyword:     Anti-acne Sheet mask Silver nanoparticles Plantago major Safety Efficacy


Avanti C, Muftilana A, Fiesta B, Rani KC, Sukweenadhi J, Kartini K, Mago RG, Devi WV, Wulansari DD, Kirtishanti A. Plantago major synthesized silver nanoparticles in an anti-acne facial sheet mask: physical stability, safety, and efficacy. J App Biol Biotech. 2024. Online First.

Copyright: Author(s). This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.

HTML Full Text

1. Heng AH, Chew FT. Systematic review of the epidemiology of acne vulgaris. Sci Rep 2020;10:5754.

2. Tan JK, Bhate K. A global perspective on the epidemiology of acne. Br J Dermatol 2015;172 Suppl 1:3-12.

3. Dréno B, Pécastaings S, Corvec S, Veraldi S, Khammari A, Roques C. Cutibacterium acnes (Propionibacterium acnes) and acne vulgaris: A brief look at the latest updates. J Eur Acad Dermatol Venereol 2018;32 Suppl 2:5-14.

4. Davis EC, Callender VD. A review of acne in ethnic skin: Pathogenesis, clinical manifestations, and management strategies. In J Clin Aesthet Dermatol 2010;3:24-38.

5. Rodan K, Fields K, Majewski G, Falla T. Skincare bootcamp: The evolving role of skincare. Plast Reconstr Surg Glob Open 2016;4:e1152.

6. Nilforoushzadeh MA, Amirkhani MA, Zarrintaj P, Moghaddam AS, Mehrabi T, Alavi S, et al. Skin care and rejuvenation by cosmeceutical facial mask. J Cosmet Dermatol 2018;17:693-702.

7. Oge' LK, Broussard A, Marshall MD. Acne vulgaris: Diagnosis and treatment. Am Fam Physician 2019;100:475-84.

8. Tobiasz A, Nowicka D, Szepietowski JC. Acne vulgaris-novel treatment options and factors affecting therapy adherence: A narrative review. J Clin Med 2022;11:7535.

9. Sukweenadhi J, Setiawan KI, Avanti C, Kartini K, Rupa EJ, Yang DC. Scale-up of green synthesis and characterization of silver nanoparticles using ethanol extract of Plantago major L. Leaf and its antibacterial potential. S Afr J Chem Eng 2021;38:1-8.

10. Liao C, Li Y, Tjong SC. Bactericidal and cytotoxic properties of silver nanoparticles. Int J Mol Sci 2019;20:449.

11. Artounian K, Bundogji N, Hoss E, Boen M. Applications of gold and silver nanoparticles in the treatment of acne vulgaris: A systematic review. J Drugs Dermatol 2021;20:666-70.

12. Salve P, Vinchurkar A, Raut R, Chondekar R, Lakkakula J, Roy A, et al. An evaluation of antimicrobial, anticancer, anti-inflammatory and antioxidant activities of silver nanoparticles synthesized from leaf extract of Madhuca longifolia utilizing quantitative and qualitative methods. Molecules 2022;27:6404.

13. Husain S, Nandi A, Simnani FZ, Saha U, Ghosh A, Sinha A, et al. Emerging trends in advanced translational applications of silver nanoparticles: A progressing dawn of nanotechnology. J Funct Biomater 2023;14:47.

14. George R, Merten S, Wang TT, Kennedy P, Maitz P. In vivo analysis of dermal and systemic absorption of silver nanoparticles through healthy human skin. Australas J Dermatol 2014;55:185-90.

15. Cekic ND, Savic SM, Savic SD. Dynamic-mechanical thermoanalysis test: A rapid alternative for accelerated freeze-thaw stability evaluation of W/O emulsions. Drug Dev Ind Pharm 2019;45:1896-906.

16. Ryu S, Han HM, Song PI, Armstrong CA, Park Y. Suppression of Propionibacterium acnes infection and the associated inflammatory response by the antimicrobial peptide P5 in Mice. PLoS One 2015;10:e0132619.

17. Kim JS, Kuk E, Yu KN, Kim JH, Park SJ, Lee HJ, et al. Antimicrobial effects of silver nanoparticles. Nanomedicine 2017;3:95-101.

18. Shah MZ, Guan ZH, Din AU, Ali A, Rehman AU, Jan K, et al. Synthesis of silver nanoparticles using Plantago lanceolata extract and assessing their antibacterial and antioxidant activities. Sci Rep 2021;11:20754.

19. Lambers H, Piessens S, Bloem A, Pronk H, Finkel P. Natural skin surface pH is on average below 5, which is beneficial for its resident flora. Int J Cosmet Sci 2006;28:359-70.

20. Collier CN, Harper JC, Cafardi JA, Cantrell WC, Wang W, Foster KW, et al. The prevalence of acne in adults 20 years and older. J Am Acad Dermatol 2008;58:56-9.

21. Noga M, Milan J, Frydrych A, Jurowski K. Toxicological aspects, safety assessment, and green toxicology of silver nanoparticles (AgNPs)-critical review: State of the art. Int J Mol Sci 2023;24:5133.

22. Ong WT, Nyam KL. Evaluation of silver nanoparticles in cosmeceutical and potential biosafety complications. Saudi J Biol Sci 2022;29:2085-94.

23. McLaughlin J, Watterson S, Layton AM, Bjourson AJ, Barnard E, McDowell A. Propionibacterium acnes and acne vulgaris: New insights from the integration of population genetic, multi-omic, biochemical and host-microbe studies. Microorganisms 2019;7:128.

24. Taká? P, Michalková R, ?ižmáriková M, Bedlovi?ová Z, Balážová ?, Taká?ová G. The role of silver nanoparticles in the diagnosis and treatment of cancer: Are there any perspectives for the future? Life (Basel) 2023;13:466.

25. Ghazwani M, Hani U, Alqarni MH, Alam A. Development and characterization of methyl-anthranilate-loaded silver nanoparticles: A phytocosmetic sunscreen gel for UV protection. Pharmaceutics 2023;15:1434

Article Metrics
34 Views 8 Downloads 42 Total



Related Search

By author names