Research Article | Volume: 4, Issue: 2, March-April, 2016

Potential use as a bio-preservative from lupin protein hydrolysate generated by alcalase in food system

Ali Osman Ghada M. El-Araby Hefnawy Taha   

Open Access   

Published:  Apr 21, 2016

DOI: 10.7324/JABB.2016.40212

In this study, we evaluated the antibacterial activity of hydrolysates from lupin protein in vitro against gram positive (Staphylococcus aureus and Bacillus subtilis) and gram negative (Pseudomonas aeruginosa and Escherichia coli) bacteria and in food system (in minced beef under refrigerated condition). Hydrolysis of lupin protein with Alcalase was monitored for 4 h. Hydrolysates obtained after 1, 2, 3 and 4h had degree of hydrolysis values of 9%, 16%, 23% and 25%, respectively. The minimal inhibitory concentration (MIC) of LPH was determined against all bacterial strains. MIC value of LPH against all bacterial strains was 100 µg ml-1. The results showed that gram positive bacteria were more sensitive than gram negative bacteria. Adding LPH at different concentrations (100, 150 and 200 µg/g) to minced beef showed antibacterial activity during storage at 4°C compared to nisin (200 µg/g) as a positive control. The results of this study showed that enzymatic hydrolysis by Alcalase at pH 7.8 and 55 °C for 240 min is an easy tool to increase the antibacterial activity of lupin protein against both gram negative and gram positive bacteria and can be used as a bio-preservative in food system. Extending the technological validity of minced beef will help avoid big losses of minced beef and enhance its chances to be incorporated into many meat products while avoiding hygienic, chemical and technological deterioration.

Keyword:     Lupin protein Antibacterial Minced beef Hydrolysis Alcalase Antibacterial activity.


Ali Osman, GhadaM. El-Araby, Hefnawy Taha., Potential use as a bio-preservative from lupin protein hydrolysate generated by alcalase in food system. J App Biol Biotech. 2016; 4 (02): 076-081. DOI: 10.7324/JABB.2016.40212

Copyright: Author(s). This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.

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1. Alzoreky N.S, Nakahara K. Antibacterial activity of extracts from some edible plants commonly consumed in Asia. International Journal of Food Microbiology. 2003; 80 (3): 223–230.

2. Shan B, Cai Y-Z, Brooks JD, Corke H. The in vitro antibacterial activity of dietary spice and medicinal herb extracts. International Journal of Food Microbiology. 2007; 117 (1): 112–119.

3. Viuda-Martos M, Mohamadyb MA, Fernández-Lópeza J et al. In vitro antioxidant and antibacterial activities of essentials oils obtained from Egyptian aromatic plants. Food Control. 2011; 22: 1715–1722.

4. Beuchat LR. In: Dillon VM, Board RG (eds.) Natural antimicrobial systems and food preservation. CAB, Wallingford, UK, 1994, p 167–180.

5. Walker JRL. In: Dillon VM, Board RG (eds.) Natural antimicrobial systems and food preservation. CAB, Wallingford, UK, 1994, pp 181–204.

6. Arora DS, Kaur J. Animicrobial activity of spices. International Journal of Antimicrobial Agents. 1999; 12: 257–262.

7. Marino M, Bersani C, Comi G. Antimicrobial activity of the essential oils of Thymus vulgaris L. measured using a bioimpedometric method. Journal of Food Protection. 1999; 62: 1017–1023.

8. Mahgoub S, Osman A, Sitohy M. Inhibition of Growth of Pathogenic Bacteria in Raw Milk by Legume Protein Esters. Journal of Food Protection. 2011; 74 (9): 1475–1481.

9. Mahgoub S, Sitohy M, Osman A. Counteracting Recontamination of Pasteurized Milk by Methylated Soybean Protein. Food and Bioprocess Technology. 2013; 6:101–109.

10. Sitohy M, Mahgoub S, Osman A. Controlling psychrotrophic bacteria in raw buffalo milk preserved at 4 °C with esterified legume proteins. LWT - Food Science and Technology. 2011; 44: 1697-1702.

11. Sitohy M, Mahgoub S, Osman A. In vitro and in situ antimicrobial action and mechanism of glycinin and itsbasic subunit. International Journal of Food Microbiology. 2012; 154: 19–29.

12. Sitohy M, Mahgoub S, Osman A, El-Masry R, Al-Gaby A. Extent and mode of action of cationic legume proteins against Listeria monocytogenes and Salmonella Enteritidis. Probiotics and Antimicrobial Proteins. 2013; 5:195–205.

13. Osman A, Mahgoub S, El-Massry R, El-Gaby A, Sitohy M. Extending the Technological Validity of Raw Buffalo Milk at Room Temperature by Esterified Legume Proteins. Journal of Food processing and preservation. 2014; 38, 223-231.

14. Osman A, Mahgoub S, Sitohy M. Preservative action of 11S (glycinin) and 7S (β-conglycinin) soyglobulin on bovine raw milk stored either at 4 or 25 °C. Journal of Dairy Research. 2013; 80: 174–183.

15. Osman A, Mahgoub S, Sitohy M.Hindering milk quality storage deterioration by mild thermization combined with methylated chickpea protein. International Food Research Journal.2014; 21(2): 693-701.

16. Pereira P, Huerta B, Borge C, Astorga R, Romero R, Perea A. 2004. Antimicrobial activity of five essential oils against origin strains of the Enterobacteriaceae family. Acta Pathologica, Microbiologicaet Immunologica Scandinavica. 2004; 113(1): 1-6.

17. Joray ML, Rayas-Duarte P, Mohamed A, Van Santen E. Coated lupin beansnacks. Journal of Food Quality. 2007; 30(2): 267–279.

18. Yeheyis L, Kijora C, Wink M, Peters KJ. Effect of a Traditional processing method on the chemical composition of local white lupin (Lupinusalbus L.) seed in north-western Ethiopia. Zeitschrift Fur Naturforschung Section C—A Journal of Biosciences. 2011; 66(7–8): 403–408.

19. Torres A, Frias J, Vidal-Valverde C. Changes in chemical composition of lupin seeds (Lupinus angustifolius) after selective alpha-galactoside extraction. Journal of the Science of Food and Agriculture. 2005; 85(14): 2468–2474.

20. Sitohy M, Osman A. Antimicrobial activity of native and esterified legume proteins against Gram-negative and Gram-positive bacteria. Food Chemistry. 2010; 120:66-73.

21. Osman A, Goda HA, Abdel-Hamid M, Badran SM, Otte J. Antibacterial peptides generated by Alcalase hydrolysis of goat whey. LWT - Food Science and Technology.2016; 65: 480-486.

22. Théolier J, Hammami R, Labelle P, Fliss I, Jean J.Isolation and identification of antimicrobial peptides derived by peptic cleavage of whey protein isolate. Journal of Functional Foods. 2013; 5: 706 –714.

23. Sukan G, Andrews AT. Application of the plastein reaction to caseins and to skim milk powder. I. Protein hydrolysis and plastein formation. Journal of Dairy Research. 1982; 49:265–278.

24. Fox PF. The milk protein system. In Developments in Dairy Chemistry. Vol. 4. P. F. Fox, ed. Elsevier Applied Science, London, UK. 1989, P 1–53.

25. Rebeca BD, Pena-Vera MT, Díaz-Castañeda M. Production of fish protein hydrolysates with bacterial proteases; yield and nutritional value. Journal of Food Science. 1991; 56: 309–314.

26. Espejo-Carpio F, De Gobba C, Guadix A, Guadix E, Otte J.Angiotensin I-converting enzyme inhibitory activity of enzymatic hydrolysates of goat milk protein fractions. International Dairy Journal. 2013; 32: 175-183.

27. Sila A, Naima N, Karima H, Gabrielle C, Rafik B, Moncef, N, Pascal D, Ali B. Antibacterial peptides from barbel muscle protein hydrolysates: Activity against some pathogenic bacteria. LWT - Food Science and Technology. 2014; 55: 183-188.

28. Johnson EA, Brekke J. Functional properties of acylated pea protein isolates. Journal of Food Science. 1983; 48: 722-725.

29. Adler-Nissen J. Enzymic hydrolysis of food proteins. Barking. UK: Elsevier Applied Science Publishers;1986.

30. Organisation of Economic Co-operation and Development (OECD). The OECD Guideline for Testing of Chemical: 420 Acute Oral Toxicity. OECD, Paris, France; 2001.

31. Nanda A, Saravanan M.Biosynthesis of silver nanoparticles from Staphylococcusaureus and its antimicrobial activity against MRSA and MRSE. Nanomedicine: Nanotechnology, Biology and Medicine. 2009; 5: 452-456.

32. Niehius WG, Samuelson B. Formation of malondialdehyde from phospholipid arachidonate during microsomal lipid peroxidation. European Journal of Biochemistry. 1968; 6: 126–130.

33. APHA Standard methods for the examination of water and waste water, 18th edition. American public health association, Washington, D. C.; 1992.

34. Lee T. The liberal media myth revisited: An examination of factors influencing; 2005.

35. A.O.A.C. Official Methods of Analysis, 18th ed. A.O.A.C. International, MD, USA (1250 p.); . 2000.

36. Mansour EH, Khalil. Evaluation of antioxidant activity of some plant extracts and their application to ground beef patties. Food Chemistry. 2000; 69:135-141.

37. Yokota S, Fujii N. Contributions of the lipopolysaccharide outer core oligosaccharide region on the cell surface properties of Pseudomonas aeruginosa. Comparative Immunology, Microbiology & Infectious Diseases.2007; 30:97–109.

38. Modi VK, Mahendrakar NS, Narasimha D, Sachindra NM. Quality of buffalo meat burger containing legume flours as binders J. Food Engineering. 2003; 66: 143-149.

39. Fernandez-Gines JM, Fernandez-Lopez J, Sayas- Barbera E, Perez-Alvarez JA. Meat products as functional foods. Food Science.2005; 70: 37-43.

40. Mahmoud AH, Yehia G, Moharram MB, Magdi OE. Frozen storage stability of beef burger containing plat meat substitutes. Food Chemistry. 1987; 26(4): 261-269.

41. Akesowan A. Quality characteristics of light pork burgers fortified with soy protein isolate. Food science and biotechnology. 2010; 19:1143–1149.

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