Research Article | Volume: 5, Issue: 6, Nov-Dec, 2017

Bioactive potential of Diadema sp. from the South East Coast of Mauritius

Lisa Karen Yee Chin Youne Ah Shee Tee Daneshwar Puchooa Vishwakalyan Bhoyroo   

Open Access   

Published:  Nov 09, 2017

DOI: 10.7324/JABB.2017.50602
Abstract

As a consequence of the alarming rise in antimicrobial resistance, this study aimed at evaluating the bioactive potential of sea urchin extract, Diadema sp. Two different tissues; shells and gonads were used and two drying methods were compared; freeze drying (FD) and non-freeze drying (NFD). Statistically significant interaction was observed between the drying methods and solvents used. Compared to the gonadal tissue, NFD methanolic shell extract reported higher extraction yield, 9.13 ± 0.87%. Phenolic content assessed using the Folin-Ciocalteau method was higher in non-freeze dried (NFD) gonadal extract, 688.1 mg GAE/g DW. Promising antimicrobial activity was recorded using agar disc diffusion and results confirmed using MIC. Among the bacteria used, Escherichia coli and Staphylococcus aureus were more vulnerable, reporting inhibition zone of 16.3 ± 1.37 mm and 17.1 ± 0.23 mm using NFDSM and FDGM extract respectively. DPPH antioxidant assay showed NFDSM having better activity than the positive control, IC50 of 3.77 µg/ml. No significant larvicidal activity was recorded. Diadema sp are a potential source of novel antimicrobial compounds and suggest its possible use as natural food-grade antioxidant. This study also shows non-freeze drying method and methanol as the recommended solvent for maximal bioactivity.


Keyword:     Sea urchin Antibacterial activity Scavenging capacity Larvicidal activity.


Citation:

Tee LKY, Puchooa D, Bhoyroo V. Bioactive potential of Diadema sp. from

the South East coast of Mauritius. J App Biol Biotech. 2017;5(6):9-13.

DOI: 10.7324/JABB.2017.50602

Copyright: Author(s). This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.

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Reference

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