Oxidative status of cryopreserved sperm of stallions of different ages
Age modifies the redox balance of stallion sperm and may determine post-thaw performance. We compared cryopreserved sperm from younger (3–5 year), mature (6–15 year), and older (16–24 year) Arabian stallions using imaging flow cytometry for total reactive oxygen species (ROS) (DCFH-DA) and superoxide (DHE), Annexin V/PI for apoptotic-like changes, JC-1 for mitochondrial membrane potential (MMP), and photometric assays for oxidative protein modifications (OMP), malondialdehyde, catalase, and superoxide dismutase. Older stallions showed higher OMP, higher catalase activity, more Annexin V-positive cells, and lower MMP, with reduced post-thaw motility; younger stallions exhibited minimal oxidative damage yet low progressive motility (PM); mature stallions had the highest total/PM consistent with a balanced redox state. These findings suggest prioritizing semen collection from mature stallions (6–15 years) to improve cryopreservation outcomes. Antioxidants are recommended for older stallions to reduce oxidative damage, while energy-boosting compounds are advised for younger stallions to improve motility. Validation of age-tailored interventions and definition of operational ROS/MMP thresholds are warranted to optimize dosing and prevent reductive stress.
Shitikova AM, Atroshchenko MM, Zvyagina VI, Aronova MA. Oxidative status of cryopreserved sperm of stallions of different ages. J Appl Biol Biotech 2025. Article in Press. http://doi.org/10.7324/JABB.2025.254712
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Rekha Kaushik, Shiv Kumar, Swarnim Thussu, Poonam Khanna, Rahul MehraPhytomolecules against inflammation and fibrosis in immunoglobulin A nephropathy
Rajiv Jash,, Kousik Maparu, Sanket Seksaria, Sujan Banerjee, D. S. N. B. K. Prasanth, Himangshu Sekhar MajiOptimization of pasteurization process of the ready-to-drink beverage from Hong Quan (Flacourtia jangomas) fruit by response surface methodology
Tan Duy Nguyen,, Tuyen Thi Xuan Vo,, Khang Nghia Tran,Cotton (Gossypium spp.) pest management in the era of next-generation sequencing: A review
Julie Rebecca Joseph Mathari, Habeeb Shaik MohideenIsolation and cloning of the Pseudomonas fluorescens chitinase gene – An ecofriendly approach for its use as a specific biopesticide
Suganthi M, Abirami G, Jayanthi M, Ashok Kumar K, Deepan S, Senthilkumar PGenoprotective potentials of two traditional medicinal plants Scoparia dulcis L. and Vernonia cinerea (L.) Less.
Jimsy Johnson, Leyon VargheseEfficacy of bacteriophage L522 against bacterial leaf blight of rice in Vietnam
Pham D.T. My,, Le T.T. Tien,, Le P. Nga,, To H. Ngoc,, Vo T. Phuc,, Hoang A. Hoang,Performance of mungbean (Vigna radiata L. Wilczek) accessions under intermittent water deficit stress in a tropical environment
Uchenna Noble Ukwu, Blessing Ngozika Oburu, Delight Promise Udochukwu,, Solomon Oluwaseyi Adewuyi, Stella Ogochukwu Muojiama, Vivian Ogechi Osadebe, Ifesinachi Nelson Ezeh, Patience Ukamaka Ishieze, Nathaniel DaudaMicrobial diversity of river Ganga at Haridwar (Uttarakhand) through metagenomic approaches
Annapurna Katara, Sumit Chand, Harshvardhan Chaudhary, Harish Chandra, Ramesh Chand DubeyContribution of the triple hermetic bag in the preservation of locally processed cereal and legume products in Niger
Issoufou Amadou,, Ibrahim Boukary Baoua, Abdoulaye SankhonMolecular, morphological, and biomolecular characterization of ethyl methanesulfonate-induced mutations in Aerides odoratum, an orchid
B. S. Jyothsna, Sanjay Dey, Venkataramanan S., Raghavendra L. S. Hallur, Deepti SrivastavaUnravelling codon usage patterns in the coding sequences of Bat RNA virus genomes of Rhabdoviridae family
Deepika Sharma, Yengkhom Sophiarani, Supriyo ChakrabortyInfectious characteristics of some Vibrio spp. phages isolated in shrimp farming of the Mekong delta
Le Hoang Bao Ngoc,, Nguyen Thi Loan Anh, Vo Ngoc Tram Anh, Nguyen Thi Phuong Uyen, Le Viet Dung, Truong Thi Bich VanEffects of liquid storage of buck semen at refrigeration temperatures on sperm viability and fertility to develop ready to use goat semen diluent
Manish Kumar,, Ravi Ranjan, Alok BhardwajTechnological advancement in the development of monoclonal antibody therapies: Present, past, and future
Harit Kasana,, Harish Chander, Ashwani MathurAssessment of protein profile and antibacterial activity in hemolymph of Bacillus thuringiensis-immunized muga silkworm
Shibani Kalita, Sanghamitra Saharia, Dimpimoni Kalita, Dhirunabh Swargiary, Sunayan BardoloiCharacterization of acid-soluble collagen from peanut worm (Siphonosoma australe) and its inhibitory activity of cyclooxygenase-2
Suwarjoyowirayatno,, Chusnul Hidayat, Tutik Dwi Wahyuningsih, Retno IndratiAn efficient method for extracting pure DNA from the oil seed crop Sesamum indicum L.
Anshuman Shah,, Pragya Mishra, Nitin Gadol, Neha Jain, Rajeev Kumar, Sanjay Kalia, Nagendra Kumar Singh, Vandna RaiSustainability analysis of the greenhouse tomato (Solanum lycopersicum L.) production system
Francisco Suazo-López,, Claudia Hernández-Aguilar, José Armando Ramírez-Arias, Rosalba Zepeda-Bautista, Martha Elena Domínguez-HernándezMonoclonal antibodies for diagnosis of viral diseases: a comprehensive review
Abhishek Kasana, Aishwarya Dwivedi, Vaishali VermaMutational enhancement of Aspergillus niger Tiegh. for higher cellulase production comparable to Trichoderma species in solid-state fermentation
Harjeet Singh, Komal Janiyani, Ajit GangawaneImpact of plant growth regulators on shortening maturity duration and enhancing grain yield in Oryza sativa cv. OM5451
Linh Minh Hong Tran,, Huong Thanh Tran,, Viet Trang Bui,Asparagus racemosus extract increases the life span in Drosophila melanogaster
K. V. Kiran Kumar, K. S. Prasanna, J. S. AshadeviImpact of Phyllanthus amarus extract on antioxidant enzymes in Drosophila melanogaster
N. Manasa, J. S. AshadeviAlterations in antioxidant defense system in hepatic and renal tissues of rats following aspartame intake
Saeed A. AlwaleediDietary Supplementation of Citric acid (monohydrate) Improves Health Span in Drosophila melanogaster
Komal Panchala, Kesha Patelb , Anand K. TiwariaBiochemical Modulations in Duttaphrynus melanostictus Tadpoles, Following Exposure to Commercial Formulations of Cypermethrin: An Overlooked Impact of Extensive Cypermethrin use
David Muniswamy, Shrinivas S Jadhav, Kartheek R MalowadeDNP induced oxidative stress on blood components ameliorated by Pyrrole derivative of Tinospora cordifolia
K. C. Rashmi, H. S. AparnaManagement of heat stress in Drosophila melanogaster with Abhrak bhasma and ascorbic acid as antioxidant supplements
Rambhadur P. Subedi, Rekha R. Vartak, Purushottam G. KaleAntioxidant and antihyperlipidemic effects of aqueous seed extract of Daucus carota L. in triton ×100-induced hyperlipidemic mice
Habibu Tijjani, Abubakar Mohammed, Sani Muktar, Saminu Musa, Yusuf Abubakar, Adegbenro Peter Adegunloye, Ahmed Adebayo Ishola, Enoch Banbilbwa Joel, Carrol Domkat Luka, Adamu Jibril AlhassanBiochemical and liver histological changes in rats exposed to sub-lethal dose of Uproot-pesticide and the protective potentials of nutritional supplements
Cosmas Onyekachi Ujowundu, Kingsley Isaac Ogamanya, Favour Ntite Ujowundu, Victoria Ojone Adejoh, Calistus I. Iheme, Kalu Okereke IgweBiochemical and ultrastructural alterations in the brain of mice induced by aqueous leaf extract of a medicinal plant, Lantana camara L. and its amelioration by nimodipine and flunarizine
H. Ashalata Singha, Mahuya Sengupta, Meenakshi BawariChronic cold exposure aggravates oxidative stress in reproductive organs of STZ-induced diabetic rats: Protective role of Moringa oleifera
Hanumanthappa Rakesh, Saumya S. Mani, Piler Mahaboob BashaCorrelates of sperm quality parameters and oxidative stress indices in diabetic rats exposed to cold stress: Role of Moringa oleifera leaf extract
Piler Mahaboob Basha, Hanumanthappa Rakesh, Saumya S. ManiLeaf senescence and its regulation with phytohormones and essential elements: An overview
Shatrupa Singh, Madhulika Singh,, Sanskriti Bisht, Jai Gopal SharmaAssessment of oxidative stress, genotoxicity, and histopathological alterations in freshwater food fish Channa punctatus exposed to fungicide, Mancozeb
Manoj Kumar, Anjali Mishra, Akash Verma, Anamika Jain, Adeel Ahmad Khan, Shikha Dwivedi, Sunil P. TrivediThe protective action of a novel DinB protease against diarrhea infection in Drosophila Melanogaster
Jyoti Guleria, Mohammad Rashid Khan, Minhaj Ahmad KhanConsumption of corn and soybean sprouts enriched with egg shell in improving oxidative stress and estrogen depletion in ovariectomized rats
Siti Aminah, Wulandari Meikawati, Sri Hartati, Diode YonataMelatonin: Possible mechanism of commercial production by cyanobacteria for human welfare and sustainable agriculture
Sakshi Pandey, Shobhit Raj Vimal, Sheo Mohan PrasadFlood stress and its effects on fruit crops: A critical review
S. R. Jervin Ananth, Sajan Kurien, V. Suresh, P. Ramesh Kumar, K. Indira Petchiammal