Research Article | Volume: 5, Issue: 1, Jan-Feb, 2017

DNP induced oxidative stress on blood components ameliorated by Pyrrole derivative of Tinospora cordifolia

K. C. Rashmi H. S. Aparna   

Open Access   

Published:  Jan 20, 2017

DOI: 10.7324/JABB.2017.50110
Abstract

The intoxications and deaths caused by 2, 4-dinitrophenol (DNP) exposure through drugs, food supplements have been frequently reported and no specific treatment has been implemented to the toxic effects of DNP in adults and children. In this study, the purified compound (TCCP), a bioactive molecule from Tinospora cordifolia exhibited radical scavenging activities. Hence, we investigated the in vitro effect of TCCP on oxidative stress induced by DNP. Blood samples from healthy donors were treated with DNP (100µM/ml) for 2h. The DNP induced oxidative damage and effect of TCCP pre-treatment were determined by assessing the serum levels of cytoplasmic, lysosomal and oxidative stress markers. TCCP effectively inhibited DNP-induced oxidative stress on blood components, which was evidenced by the levels of endogenously generated reactive oxygen species and hydrogen peroxide. In addition, the depletion of GSH (reduced glutathione) content in DNP-treated cells was prevented by TCCP. Also, experimental results reflected the decreased expression of inflammation marker such as lipid peroxidase, catalase, LDH (lactate dehydrogenase), and phosphatase levels in cells with TCCP pre incubated DNP treated group. Therefore, these studies suggest the use of TCCP as an antioxidant and anti-inflammatory agent.


Keyword:     Tinospora cordifolia Pyrrole DNP oxidative stress.


Citation:

Rashmi KC, Aparna HS. DNP induced oxidative stress on blood components ameliorated by Pyrrole derivative of Tinospora cordifolia. J App Biol Biotech. 2017; 5 (01): 059-065. DOI: 10.7324/JABB.2017.50110

Copyright: Author(s). This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.

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