Research Article | Volume: 2; Issue: 1, Jan-Feb, 2014

Nanocomposites based on bacterial cellulose in combination with osteogenic growth peptide for bone repair: cytotoxic, genotoxic and mutagenic evaluations

Raquel Mantuaneli Scarel-Caminagaa Sybele Saskab Leonardo Pereira Franchic Raquel A. Santose Ana Maria Minarelli Gaspara Ticiana S.O. Capotea Sidney José Lima Ribeirob Younés Messaddeqb Reinaldo Marchetto b Catarina S. Takahashic d   

Open Access   

Published:  Feb 25, 2014


Bacterial cellulose membranes functionalized with hydroxyapatite or collagen with addition or not of osteogenic growth peptides (OGP) or its C-terminal pentapeptide OGP[10-14] were developed for improving bone repair. The aim of this study was to evaluate the potential cytotoxic, genotoxic and mutagenic effects of those nanocomposites in order to know whether they would be safe for biomedical applications. All nanocomposites (BC, BC-HA, BC-Col, BC-HA OGP, BC-Col-OGP, BC-HA OGP[10-14] and BC-Col-OGP[10-14]) were prepared as discs (5 mm in diameter) and submitted to in vitro tests in 24-well plates seeded with CHO-K1 cells. Cell viability was evaluated by the XTT assay and reproductive cell death was detected by the clonogenic assay. Genotoxicity was assessed by the comet assay and the cytokinesis-blocked micronucleus (CBMN) assay was used to detect mutagenicity. Only BC-HA OGP[10-14] showed a slight mutagenic effect, all other nanocomposites materials demonstrated no cytotoxic, genotoxic or mutagenic effects. In conclusion, the BC-HA OGP [10-14] promoted a slight mutagenic effect and future studies must be investigated for better understanding this result. The utilization of the investigated materials is promising for biomedical applications, such as bone repair and tissue engineering.

Keyword:     Bacterial cellulosehydroxyapatitecollagencytotoxicitymutagenicity testspeptide.


Raquel Mantuaneli Scarel-Caminaga, Sybele Saska, Leonardo Pereira Franchi, Raquel A. Santos, Ana Maria Minarelli Gaspar, Ticiana S.O. Capote, Sidney José Lima Ribeiro, Younés Messaddeq, Reinaldo Marchetto, Catarina S. Takahashi. Nanocomposites based on bacterial cellulose in combination with osteogenic growth peptide for bone regeneration: cytotoxic, genotoxic and mutagenic evaluations. J App Biol Biotech. 2014; 2 (01): 001-008.

Copyright: Author(s). This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.

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