Published:  Apr 21, 2016DOI: 10.7324/JABB.2016.40209
Citric acid is a biochemical compound present in variety of citrus fruits. Citrate plays a vital role in production of energy (ATP) via Tricarboxylic acid (TCA) cycle and also involve in Fatty acid (FA) synthesis in cytoplasm. The objective of this study is to evaluate the effect of citric acid supplementation on health span in Drosophila melanogaster. OregonR+ fly used in most of the experiments. Body weight of male and female flies fed on normal and citric acid mixed diet was determined, locomotor assay and anti-oxidant activity associated with citric acid was measured by performing superoxide dismutase (SOD) and catalase (CAT) enzymatic assay. Fat content in citric acid fed flies was evaluated by Oil Red O staining in fat body tissues of adult Drosophila. Reverse trascriptase PCR (RT-PCR) analysis was performed to check the expression level of Brummer (bmm) and Fatty acid Synthase (FAS) genes. In the present study we have shown that supplementation of citric acid reduces body weight of male and female flies, improves locomotor activity of Alzheimeric flies, reduces oxidative stress and increases FA synthesis in Drosophila. Here, we concluded that supplementation of citric acid (monohydrate) improves health span in Drosophila.
Panchal K, Patel K, Tiwari AK. Dietary Supplementation of Citric acid (monohydrate) Improves Health Span in Drosophila melanogaster. J App Biol Biotech. 2016; 4 (02): 060-066. DOI: 10.7324/JABB.2016.40209
1. Kliewer WK. Sugars and Organic Acids of Vitis Vinifera. Plant Physiol. 1996; 41: 923-931.
2. Ellis H, Riki Van DB, Jaap N, Gunter F. Biosynthesis and root exudation of citric and malic acids in phosphate-starved rape plants. New Phytologist. 1992; 122: 675-680.
3. Wachman A, Bernstein DS. Diet and osteoporosis. The Lancet. 1968; 291: 958-959.
4. Beeckmans S. Some structural and regulatory aspects of citrate synthase. Int J Biochem. 1984; 16: 341-51.
5. Paul AS. The molecular physiology of citrate. Nature. 1965; 205: 766-770.
6. Mycielska ME, Patel A, Rizaner N, Mazurek MP, Keun H, Patel A, Ganapathy V, Djamgoz MB. Citrate transport and metabolism in mammalian cells Prostate epithelial cells and prostate cancer. BioEssays. 2009; 31: 10–20.
7. Saha AK, Vavvas D, Kurowski TG, Apazidis A, Witters LA, Shafrir E, Ruderman NB. Malonyl-CoA regulation in skeletal muscle: its link to cell citrate and the glucose-fatty acid cycle. Am J Physiol. 1997; 272: E641-8.
8. Ohlrogge JB, Jaworski JG, Post-Beittenmiller D. De novo fatty acid biosynthesis, In: Moore TS, Editors. Lipid Metabolism in Plants. Boca Raton, FL: CRC Press. 1993. pp. 3–32.
9. Casani S, Gómez-Pastor R, Matallana E, Paricio N. Antioxidant compound supplementation prevents oxidative damage in a Drosophila model of Parkinson's disease. Free Radic Biol Med. 2013; 61: 151-60.
10. Missirlis F, Rahlfs S, Dimopoulos N, Bauer H, Becker K, Hilliker A, Phillips JP, Jackle H. A putative glutathione peroxidase of Drosophila encodes a thioredoxin peroxidase that provides resistance against oxidative stress but fails to complement a lack of catalase activity. Biol Chem. 2003; 384:463-472.
11. Serkan Y, Fatma U, Deniz, Huseyin A. Clastogenic effects of food additive citric acid in human peripheral lymphocytes. Cytotechnology. 2008; 56: 137-144.
12. Prüßing K, Voigt A, Schulz JB. Drosophila melanogaster as a model organism for Alzheimer's disease. Mol Neurodegener. 2013; 22:8-35.
13. Sidow A, Thomas WK. A molecular evolutionary framework for eukaryotic model organism. Curr Biol. 1994; 4: 596-603.
14. Rubin GM, Lewis EB. A brief history of Drosophila’s contributions to genome research. Science. 2000; 287: 2216–2218.
15. Adams MD, Celniker SE, Holt RA, Evans CA, Gocayne JD, Amanatides PG, et al. The Genome Sequence of Drosophila melanogaster. Science. 2000; 287: 2185-2195.
16. HYE C, Bonini NM. Drosophila models of human neuro-degenerative disease. Cell Death Differ. 2000; 7: 1075-1080.
17. Tiwari AK, Pragya P, Ravi Ram K, Chowdhuri DK. Environmental chemical mediated male reproductive toxicity: Drosophila melanogaster as an alternate animal model. Theriogenology. 2011; 76: 197-216.
18. Kumar A, Dave M, Pant DC, Laxkar R, Tiwari AK. Vinca rosea leaf extract supplementation leads to developmental delay and several phenotypic anomalies in Drosophila melanogaster. Toxicol. & Environ. Chem. 2013; 95: 635-645.
19. Pant DC, Dave M, Tiwari AK. Wheatgrass (Triticum aestivum L.) Supplementation Promotes Longevity in Drosophila melanogaster. Annals of Plant Sciences. 2013; 02: 49-54.
20. Hirth F. Drosophila melanogaster in the Study of Human Neurodegeneration. CNS Neurol Disord Drug Targets. 2010; 9: 504-523.
21. Van DD, De Deyn PP. Animal models in the drug discovery pipeline for Alzheimer’s disease. Br J Pharmacol. 2011; 164: 1285–1300.
22. Feany MB, Bender WW. A Drosophila model of Parkinson's disease. Nature. 2000; 404: 394-8.
23. Kajari D, Luna S, Chainy GBN. A modified spectrophotometric assay of superoxide dismutase using nitrite formation by superoxide radicals. Ind. J Biochem & Biophy. 1999; 37: 201-204.
24. Rukmini MS, D’Souza B, D’Souza V. Superoxide dismutase and catalase activities and their correlation with malondialdehyde in schizophrenic patients. Ind. J Clin. Biochem. 2004; 19;114-118.
25. Banerjee KK, Ayub C, Sengupta S, Kolthur-Seetharam U. dSir2 deficiency in the fatbody, but not muscles, affects systemic insulin signaling, fat mobilization and starvation survival in flies. Aging (Albany NY). 2012; 4: 206-23.
26. Bus JS, Gibson JE. Paraquat: model for oxidant-initiated toxicity. Environ Health Perspect. 1984; 55: 37–46.
27. Grönke S, Mildner A, Fellert S, Tennagels N, Petry S, Müller G, Jäckle H, Kühnlein RP. Brummer lipase is an evolutionary conserved fat storage regulator in Drosophila. Cell Metab. 2005; 1: 323-30.
28. Estela A, Soulages JL. Insect fat body: energy, metabolism and regulation. Annu Rev Entomol. 2010; 55: 207–225.
29. Aquilina G, Vasileios B, Lourdes BMD, et al. Scientific Opinion on the safety and efficacy of citric acid when used as a technological additive (acidity regulator) for all animal species. EFSA. 2015; 13: 4010.
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