Research Article | Volume: 4, Issue: 2, March-April, 2016

Dietary Supplementation of Citric acid (monohydrate) Improves Health Span in Drosophila melanogaster

Komal Panchala Kesha Patelb Anand K. Tiwaria   

Open Access   

Published:  Apr 21, 2016

DOI: 10.7324/JABB.2016.40209
Abstract

Citric acid is a biochemical compound present in variety of citrus fruits. Citrate plays a vital role in production of energy (ATP) via Tricarboxylic acid (TCA) cycle and also involve in Fatty acid (FA) synthesis in cytoplasm. The objective of this study is to evaluate the effect of citric acid supplementation on health span in Drosophila melanogaster. OregonR+ fly used in most of the experiments. Body weight of male and female flies fed on normal and citric acid mixed diet was determined, locomotor assay and anti-oxidant activity associated with citric acid was measured by performing superoxide dismutase (SOD) and catalase (CAT) enzymatic assay. Fat content in citric acid fed flies was evaluated by Oil Red O staining in fat body tissues of adult Drosophila. Reverse trascriptase PCR (RT-PCR) analysis was performed to check the expression level of Brummer (bmm) and Fatty acid Synthase (FAS) genes. In the present study we have shown that supplementation of citric acid reduces body weight of male and female flies, improves locomotor activity of Alzheimeric flies, reduces oxidative stress and increases FA synthesis in Drosophila. Here, we concluded that supplementation of citric acid (monohydrate) improves health span in Drosophila.


Keyword:     Drosophila melanogaster Citric acid monohydrate Body weight Locomotor Oxidative stress Fatty acid synthesis.


Citation:

Panchal K, Patel K, Tiwari AK. Dietary Supplementation of Citric acid (monohydrate) Improves Health Span in Drosophila melanogaster. J App Biol Biotech. 2016; 4 (02): 060-066. DOI: 10.7324/JABB.2016.40209

Copyright: Author(s). This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.

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