Mycelium and exopolysaccharide production and bioactivities of select cup mushrooms (Sarcoscyphaceae, Pezizomycetes) from Central Luzon, Philippines

Eleonor Dumayas Alfonso Rich Milton R. Dulay Sofronio P. Kalaw   

Open Access   

Published:  Mar 21, 2026

DOI: 10.7324/JABB.2026.658059
Abstract

Mushrooms are recognized as important sources of bioactive compounds with antioxidant, antidiabetic, and neuroprotective properties. However, cup mushrooms remain relatively understudied despite their potential for producing unique secondary metabolites. This study evaluated the effects of culture medium, pH, temperature, agitation, and incubation period on the mycelial biomass production of Sarcoscypha spp., Phillipsia domingensis, and Cookeina tricholoma, and assessed their chemical profiles and bioactivities. Among the culture media tested, rice bran broth supported the highest biomass for Sarcoscypha spp. (326.3 mg 100/mL) and P. domingensis (342.3 mg 100/mL), whereas coconut water was optimal for C. tricholoma (312 mg 100/mL). Maximum growth occurred at pH 6.0, with optimal temperatures of 20°C for Sarcoscypha spp. and P. domingensis and 30°C for C. tricholoma, and agitation at 150 rpm significantly enhanced biomass accumulation. Peak biomass was achieved after 20 days for Sarcoscypha spp. (1585 mg 100/mL) and P. domingensis (1293 mg 100/mL), and 10 days for C. tricholoma (1314 mg 100/mL). Thin-layer chromatography analysis revealed essential oils, coumarins, and anthrones in all species; triterpenes and sterols in P. domingensis and C. tricholoma; alkaloids in Sarcoscypha spp. and P. domingensis; steroids in Sarcoscypha spp. and C. tricholoma; and phenols, tannins, and flavonoids exclusively in P. domingensis. 2,2-diphenyl-1-picrylhydrazyl radical scavenging activity ranged from 17.17–57.42% for mycelial extracts and 40.96–53.20% for exopolysaccharides. Mycelial extract of Sarcoscypha spp. at 10 μg/mL exhibited the highest α-glucosidase inhibition (23.03%) and acetylcholinesterase inhibition (12.74%), indicating its promising antidiabetic and neuroprotective potential. These findings underscore the biotechnological significance of cup mushrooms as sources of antioxidant, antidiabetic, and neuroprotective metabolites.


Keyword:     Antidiabetic Antioxidant Cup mushrooms Neuroprotective Sarcoscypha spp. Submerged cultivation


Citation:

Alfonso ED, Dulay RMR, Kalaw SP. Mycelium and exopolysaccharide production and bioactivities of select cup mushrooms (Sarcoscyphaceae, Pezizomycetes) from Central Luzon, The Philippines. J Appl Biol Biotech 2026. Article in Press. http://doi.org/10.7324/JABB.2026.658059

Copyright: Author(s). This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.

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Impact of oxidizing, reducing, and stabilizing agents on the inhibitory properties of Cyamopsis tetragonoloba trypsin inhibitor

Preeti Patidar, Mahima Golani, Sumati Hajela, Krishnan Hajela

Identification of highest L-Methioninase enzyme producers among soil microbial isolates, with potential antioxidant and anticancer properties

D. Kavya, Varalakshmi Kilingar Nadumane

Astaxanthin: An algae-based natural compound with a potential role in human health-promoting effect: An updated comprehensive review

Jinu Medhi, Mohan Chandra Kalita

Linalool protects hippocampal CA1 neurons and improves functional outcomes following experimental ischemia/reperfusion in rats

Vishal Airao, Prakruti Buch, Tejas Sharma, Devendra Vaishnav, Sachin Parmar

Modification of the time of incubation in colorimetric method for accurate determination of the total antioxidants capacity using 2,2-diphenyl-1-picrylhydrazyl stable free radical

Abhipsa Bal, Samar Gourav Pati, Falguni Panda, Biswaranjan Paital

HR-LC-MS based profiling of phytochemicals from methanol extracts of leaves and bark of Myristica dactyloides Gaertn. from Western Ghats of Karnataka, India

Kuppuru Mallikarjunaiah Marulasiddaswamy, Bettadapura Rameshgowda Nuthan,, Channarayapatna-Ramesh Sunilkumar, Shrisha Naik Bajpe,, Kigga Kaadappa Sampath Kumara, Shailasree Sekhar, Kukkundoor Ramachandra Kini

Preclinical evaluation of anticataract activity of Mentha spicata leaves on isolated goat lens by an in vitro model

Shreya Mohandas, Saahiba Thaleshwari, Myrene Roselyn Dsouza

Green synthesis, characterizations, and in vitro biological evaluation of Cu (II) complexes of quercetin with N ^ N ligands

Tanu Srivastava, Sunil Kumar Mishra, Om Prakash Tiwari

Optimization of extraction conditions of phytochemical compounds in “Xiem” banana peel powder using response surface methodology

Ngo Van Tai, Mai Nhat Linh, Nguyen Minh Thuy

Antioxidative, antiproliferative, and apoptosis effect of Coleus tuberosus flesh and peel ethanol extracts on cervical cancer cell lines

Mutiara Nugraheni, Windarwati Windarwati, Badraningsih Lastariwati

Comparative study of hydroalcoholic extracts of Bryophyllum pinnatum and Macrotyloma uniflorum for their antioxidant, antiurolithiatic, and wound healing potential

Chetna Faujdar, Priyadarshini

Qualitative and quantitative analysis of Precocene II, estimation of enzymatic, nonenzymatic antioxidant, and cytotoxic potentials of methyl jasmonate-elicited shoot culture of Ageratum conyzoides Linn.

Selvaraj Vasantharani, Ramaraj Thirugnanasampandan, Gunasekaran Bhuvaneswari

Suppression of the RAGE gene expression in RAW 264.7 murine leukemia cell line by ethyl acetate extract of Mikania micrantha (L.) Kunth.

Alex Zohmachhuana, Malsawmdawngliana Tlaisun, Vabeiryureilai Mathipi, Lalrinzuali Khawlhring, Joyce Sudandara Priya

A review on fish peptides isolated from fish waste with their potent bioactivities

Ayusman Behera, Rajashree Das, Pranati Patnaik, Jyotirmaya Mohanty, Gargee Mohanty

Quantification of phytochemicals and in vitro antioxidant activities from various parts of Euphorbia neriifolia Linn.

Priya Chaudhary, Pracheta Janmeda

Influence of soaking and germination treatments on the nutritional, anti-nutritional, and bioactive composition of pigeon pea (Cajanus cajan L.)

Qurat Ul Eain Hyder Rizvi, Krishan Kumar, Naseer Ahmed, Ajar Nath Yadav, Divya Chauhan, Priyanka Thakur, Sumaira Jan, Imran Sheikh

Impact of diverse processing treatments on nutritional and anti-nutritional characteristics of soybean (Glycine max L.)

Priyanka Thakur, Krishan Kumar, Naseer Ahmed, Ajar Nath Yadav, Sunil Kumar, Qurat Ul Eain Hyder Rizvi, Divya Chauhan, Sumaira Jan

Cathelicidin-HR from Hoplobatrachus rugulosus: an antioxidant peptide that performs a protective effect against UV/H2O2 -induced DNA damage

Piyachat Wiriyaampaiwong, Chutima Karnmongkol, Arpaporn Punpad, Nattapong Srisamoot, Wutti Rattanavichai, Alongkod Tanomtong, Sakda Daduang,, Sompong Klaynongsruang,, Anupong Tankrathok,

Woodfordia fruticosa (Linn.) Kurz’s fungal endophyte Mucor souzae’s secondary metabolites, kaempferol and quercetin, bestow biological activities

Kavyashree Doreswamy, Priyanka Shenoy, Sneha Bhaskar, Ramachandra K. Kini, Shailasree Sekhar

Characterization of the crude extract of Portulaca oleracea and the determination of the polyphenol oxidase kinetics in the presence of Cu and Zn

Omar Mohammad Atrooz, Shada Zaher Al-Maitah

Effect of diverse fermentation treatments on nutritional composition, bioactive components, and anti-nutritional factors of finger millet (Eleusine coracana L.)

Sumaira Jan, Krishan Kumar, Ajar Nath Yadav, Naseer Ahmed, Priyanka Thakur, Divya Chauhan, Qurat-Ul-Eain Hyder Rizvi, Harcharan Singh Dhaliwal

Optimization of active antioxidative defatted Canarium indicum L. (Canary) protein hydrolysate production

Cintya Nurul Apsari,, Ilma Nugrahani, Sukrasno, Tutus Gusdinar

Elemental, nutritional, and phytochemical profiling and antioxidant activity of Cordia obliqua Willd. (Clammy Cherry): An important underutilized forest tree of East India

Mamta Naik#,, Shashikanta Behera#,,, Sadhni Induar, Swaraj K. Babu, Pradeep K. Naik

Effects of enzymatic hydrolysis on the antioxidant activity of protein hydrolysate derived from the larvae of black soldier fly (Hermetia illucens L.)

Muhammad Yusuf Abduh,, Diah Ayu Prawitasari,, Ula Aulia Fitrian,, Mochamad Firmansyah,

Evaluation of functional characteristics of roselle seed and its use as a partial replacement of wheat flour in soft bread making

Nguyen Minh Thuy, Nguyen Bao Tram, Dinh Gia Cuong, Huynh Khanh Duy, Ly Thanh An, Vo Quoc Tien, Tran Ngoc Giau, Ngo Van Tai

Total phenolic, flavonoid contents, and antioxidant activity of three selected Portulaca grandiflora mutants in MV8 generation as a result of recurrent irradiation technique

Waras Nurcholis,, Syarifah Iis Aisyah, Regina Agritena Mayrischa Saraswati, Yoshua Shandy Yudha

Insights into the impact of spermidine in reducing salinity stress in Gerbera jamesonii

Javeria Uzma, Sai Krishna Talla, Praveen Mamidala

In vitro antioxidant and antibacterial potential of biosynthesized yttrium oxide nanoparticles using floral extract of Illicium verum

Karthikeyan Kandasamy, Premkumar Kumpati

Effect of combined NPK fertilizer on polyphenol contents and antioxidant activity in methanol extract of Curcuma xanthorhiza

Minarni Minarni, Rayandra Asyhar, Amira Amandanisa, Sintya Ainun, Yoshua Shandy Yudha, I Made Artika,, Waras Nurcholis,

Dehydration kinetics of green banana slices, characterization of optimized product based on physicochemical, nutritional, optical, and sensory attributes

Ram Kaduji Gadhave, Ravneet Kaur, Rahul Das, Kamlesh Prasad

Assessment of morpho-agronomic and yield attributes in gamma-irradiated mutants of Kalanamak rice (Oryza sativa L.)

Tanmai Mishra, Anjali Singh, Virendra Kumar Madhukar, Ashutosh Kumar Verma, Shambhavi Mishra, Rajveer Singh Chauhan

Metabolic profile, bioactivities, and variations in chemical constituents of essential oils of twenty mango ginger (Curcuma amada) accessions

Jyotirmayee Lenka, Snehalata Khuntia, Basudeba Kar, Suprava Sahoo

Phenolic compounds and in vitro antioxidant activity of spray-dried and freeze-dried aqueous extracts of sea cucumber (Holothuria tubulosa)

Fadna Aatab, Fatima Bellali, Fatima Zahra Aboudamia, Ahmed Errhif, Mariem Kharroubi

Optimization of pasteurization process of the ready-to-drink beverage from Hong Quan (Flacourtia jangomas) fruit by response surface methodology

Tan Duy Nguyen,, Tuyen Thi Xuan Vo,, Khang Nghia Tran,

Increasing polyphenol antioxidant in Orthosiphon stamineus Benth leaves with fermentation extraction by Saccharomyces cerevisiae ATCC-9763

Muhammad Aria Chandra, Khaswar Syamsu, Laksmi Ambarsari, Nurul Fatimah, Waras Nurcholis,

Phytochemical composition and antiproliferative activity of Opuntia elatior Mill.: In vitro and in silico studies on breast cancer cell line MCF-7

Foram Patel, Khushali Upadhyay, Denni Mammen, Elizabeth Robin, A.V. Ramachandran, Darshee Baxi

Bioactive properties of the extracts of peels, pomace, seeds, and essential oils of Citrus limon and Citrus aurantifolia

Folasade Oluwatobi, Olakunle Afolabi, Pius Okiki, Funmilayo Adeniyi, Oghenerobor Akpor

High resolution-liquid chromatograph mass spectrometer characterization of bioactive compounds in pineapple wastes: Valorization of antioxidant and enzymatic activity

Suman Polaki, Sourav Nayak, K. Sampad Kumar, Rabi Prasad B

Recent important insight into nutraceuticals potential of pigmented rice cultivars: A promising ingredient for future food

Le Thi Kim Loan, Bui The Vinh, Ngo Van Tai

Solid-state fermentation of pigment producing endophytic fungus Fusarium solani from Madiwala lake and its toxicity studies

Bhoomika Prakash Poornamath, Suma Sarojini, Saranya Jayaram, Soma Biswas, Anand Kaloor, Mridul Umesh

Elucidation of antioxidant compounds recovery capacity from “Cam” purple rice bran by different sustainable extraction techniques

Le Thi Kim Loan, Bui The Vinh, Ngo Van Tai

Secondary metabolite profiles, antimicrobial and antioxidant activities of callus, and leaves extract of Piper sarmentosum Roxb.

Junairiah Junairiah, Listijani Suhargo, Tri Nurhariyati, Nabilah Istighfari Zuraidassanaaz

Assessment of in vitro antioxidant properties and anticancer potential of Cucumis pubescens Willd. a medicinal fruit, utilizing human lung cancer cell line (A549)

T. Sundari, R. Kavitha, B. Mythili Gnanamangai, S. Saranya

Exploring Bougainvillea glabra flowers: a promising source of natural antimicrobial and anticancer agents

Wanchat Sirisarn, Auemphon Mordmuang, Kankamol Kerdkumthong, Sompop Saeheng,,

The effectiveness of the use of antioxidant formulations in the storage of fat from the Pacific sardines Sardinops melanostictus

Oksana V. Tabakaeva, Lidia V. Shulgina,, Mouhamad Alrajab, Anton V. Tabakaev, Pavel A. Shinkaruk, Varvara D. Stepochkina

Evaluation and characterization of endophytic bacteria from Capparis decidua (Forssk.) Edgew. for their antifungal and antioxidant activities

Sudesh Kumari, Prity Gulia, Pooja Choudhary, Ritu Pasrija, Mehak Dangi, Anil Kumar Chhillar

Sustainable improvement of nutrition quality and biological activity from cassava residue and okara through solid-state fermentation by Pleurotus citrinopileatus mycelium

Hang Nguyen Thi Bich, Cuong Chi Doan, Uyen Nguyen Khanh Phan, Khanh Trang Vu Le, Thang Duc Bui, Munehiro Tanaka, Minh Van Vo

Green synthesised Catharanthus roseus-mediated iron oxide nanoparticles demonstrates enhanced antibacterial, antioxidant, and anti-diabetic properties

Priya Josson Akkara, Sonia Angeline Martin, Nathiya Thiagarajulu, Aanchal Bisht Bisht, Archana Mishal, Christina M. Mathew

Melatonin: Possible mechanism of commercial production by cyanobacteria for human welfare and sustainable agriculture

Sakshi Pandey, Shobhit Raj Vimal, Sheo Mohan Prasad

Selenium-enriched Bacillus siamensis and Staphylococcus arlettae isolated from fermented rice

Nandhini Govindaraj, Silambu Selvi Kumbamoorthy, Vishnupriya Subramaniyan, Sundaresan Sivapatham

Effects of triclosan at different salinity levels on swimming behavior, reproduction, and antioxidant status in the marine rotifer Brachionus plicatilis

Ojeshvi Ethiraj, Arjun Surya Subramanya, Abdul Raziq Raheem, Jaganathan Murugesan Kuppuswamy

Biostimulant effect of hydrolyzed Spirulina (Ficocyan®) on bioactive compounds in pigmented corn from Jalisco, Mexico

Raúl Armando Chavarín González, Valeria Yadira Ortiz Sosa, Ramón Rodríguez Macias, Ricardo Solis Zamora, Luis Alberto Anguiano Sevilla, Juan Carlos Pizano Andrade, Mario Alberto Ruiz López

Phytochemical profiling and in silico target exploration of hexadecanoic acid from Bergenia ciliata: An integrative approach combining docking, absorption, distribution, metabolism, and excretion, and biological activity studies

Sagar Vishwakarma, Vijeta Chaudhry, Sumit Chand, Soban Prakash, Kajal Singh, Ajay Singh, Rajendra Prasad, Harish Chandra

Microalgae: A valuable bio-resource for pharmaceuticals and nutraceuticals

Sanjesh Tiwari, Vandana Sharma, Anuradha Patel, Vaishali Yadav, Charu Kalra, Garima Singh, Namira Arif, Arushi Khandelwal, Anupam Tiwari, Dileep Kumar Singh

Eco-friendly fabrication of silver nanoparticles from the red macroalga Scinaia moniliformis and evaluation of their biomedical activities

Hemangiben Sheth, Charumati Jha

Phytochemical and ethnobotanical investigation of 29 aromatic and medicinal plants from Dakhla-Oued Ed-Dahab region and their traditional uses

Hajar Sadki, Oukacha Amri, Rachid Ait Taher, Khadija El Mehrach, Fadma Fahmi, Hakima Lounis, Ouafae Benkhnigue, Brahim Alayoua, Saida Tahrouch

Prebiotic properties of polysaccharide extracts from Cordyceps militaris mycelium: Potentials for functional food and drink applications

Hang Bich-Thi Nguyen,, Cuong Chi Doan, Thang Duc Bui, Nhat Minh Dang

Xeno-estrogenic metal-induced alterations in antioxidant enzyme activities, vitellogenin, and estrogen-receptor alpha in Channa striata brooders

Nazura Usmani, Muizzah Fatima

In vitro antioxidant and acetylcholinesterase activities of catechin-loaded green fabricated zinc oxide nanoparticles

Nandhini Baskaran, Anitha Subash