Published:  Oct 23, 2016DOI: 10.7324/JABB.2016.40508
Diabetes mellitus is a chronic disorder of carbohydrate, protein, fat and mineral metabolism. The antidiabetic potential of aqueous extracts flower and root of Aerva lanata was evaluated in alloxan induced diabetic rats. Alloxan (150 mg/kg bw) induced diabetic rats showed a hyperglycemic state that led to various biochemical and metabolic alterations. The aqueous extract of flower and root of A. lanata (200 mg/kg bw) was administrated orally to diabetic rats for 45 days. In this study, the levels of fasting blood glucose, glycated hemoglobin (HbA1C), C-reactive protein (CRP), total cholesterol, triglycerides (TG), low density lipoprotein (LDL), very low density lipoprotein (VLDL), urea, and creatinine were significantly increased in diabetic rats. Body weight, hemoglobin, insulin, C-peptide and protein levels in blood were significantly decreased in alloxan induced diabetic rats. After the administration of extracts of flower and root of A. lanata to diabetic rats showed significantly restore the levels as normal rats. The antidiabetic effect of A. lanata was compared with strandard drug glibenclamide (1 mg/kg bw) treated rats. From this study, we concluded that the aqueous extract of A. lanata root have more antidiabetic effect when compared with flower extract. Therefore, the flower and root of A. lanata may be used as the alternative medicine for the treatment of diabetes mellitus.
Vidhya R, Udayakumar R. Hypoglycemic and hypolipidemic effects of Aerva lanata (Linn.) on alloxan induced diabetic rats. J App Biol Biotech. 2016; 4 (05): 048-056. doi: 10.7324/JABB.2016.40508
1. Grover JK, Yadav S, Vats V. Medicinal plants of India with antidiabetic potential. Journal of Ethnopharmacology. 2002; 81: 81-100.
2. Scartezzini P, Sproni E. Review on some plants of Indian traditional medicine with antioxidant activity. Journal of Ethnopharmacology. 2000; 71: 23-43.
3. Seth SD, Sharma B. Medicinal plants of India. Indian Journal of Medical Research. 2004; 120: 9-11.
4. Ramachandran A, Snehalatha C, Viswanathan V. Burden of type II diabetes and its complications- the Indian scenario. Current Science. 2002; 83: 1471-1476.
5. Huang THW, Peng G, Kota BP, Li GQ, Yamahara J, Roufogalis BD et al. Anti-diabetic action of Punica granatum flower extract: activation of PPAR-c and identification of an active component. Toxicology and Applied Pharmacology. 2005; 207:160-169.
6. Kumar P, Clark M. Diabetes Mellitus and Other Disorders of Metabolism. In: Clinical Medicine, Sunders WB, editors. 2nd ed. Elsevier, London: 2002, p.1069-1071.
7. Burke JP, Williams K, Narayan KMV, Leibson C, Haffner SM, Stern MP. A population perspective on diabetes prevention: Whom should we target for preventing weight gain. Diabetes Care. 2003; 26:1999- 2004.
8. Mahabir D, Gulliford MC. Use of medicinal plants for diabetes in Trinidad and Tobago. Revista Panamericana de Salud Publica. 1997; 1:174-179.
9. Deepashree BN, Prakash JA. Study on nutritional status of diabetics and associated risk factors. Journal of Human Ecology. 2007; 21: 269-274.
10. Malviya N, Jain S, Malviya S. Antidiabetic potential of medicinal plants. Acta Poloniae Pharmaceutica - Drug Research. 2010; 67(2):113-118.
11. Rajalakshmi M, Eliza J, Priya CE, Nirmala A, Daisy P. Antidiabetic properties of Tinospora cordifolia stem extracts on streptozotocin-induced diabetic rats. African Journal of Pharmacy and Pharmacology. 2009; 3(5):171-180
12. Krishnamurthi A. The wealth of India. Vol. I, CSIR, New Delhi, 2003, p. 92.
13. Kiritikar KR, Basu BD. Indian Medicinal Plants. 1996, p. 2064-2065.
14. Dulaly C. Antimicrobial activity and cytotoxicity of Aerva lanata. Fitoterapia. 2002; 73: 92-94.
15. Udupihille M, Jiffry MTM. Diuretic effect of Aerva lanata with water, normal saline and coriander as controls. Indian Journal of Physiology and Pharmacology. 1986; 30: 91-97.
16. Rao SG. Evaluation of an experimental model for studying urolithiasis effect of Aerva lanata on urinary stones. Indian Drugs. 1985; 22: 640-643.
17. Vetrichelvan T, Jegadeesan M, Senthil Palaniappan S, Murali NP, Sasikumar K. Diuretic and anti inflammatory activities of Aerva lanata in rats. Indian Journal of Pharmaceutical Sciences. 2000; 62:300-302.
18. Zapesochnaya G. Canthin-6-one and beta-carboline alkaloids from Aerva lanata. Planta Medica. 1992; 58: 192-196.
19. Deshmukh TA, Yadav BV, Badole SL, Bodhankar SL, Dhaneshwar SR. Antihyperglycaemic activity of alcoholic extract of Aerva lanata (L.) A. L. Juss. Ex J. A. Schultes leaves in alloxan induced diabetic mice. Journal of Applied Biomedicine. 2008; 6: 81-87.
20. Parekh J, Nair R Chanda S. Preliminary screening of some folkloric plants from western India for potential antimicrobial activity. Indian Journal of Pharmacology. 2005; 37: 408-09.
21. Organization for Economic Cooperation and Development. The OECD 423 guideline for testing of chemicals acute oral toxicity-Acute Toxic Class Method. Paris: France; 2001.
22. Du Vigneaud V, Karr WG. Carbohydrate utilization, rat of disappearance of D-glucose from the blood. Journal of Biological Chemistry. 1925; 66: 281-300.
23. Folin 0, Wu H. A system of blood analysis. Journal of Biological Chemistry. 1919; 38: 81.
24. Nayak SS, Pattabiraman TN. A new colorimetric method for the estimation of glycosylated haemoglobin. Clinica Chimica Acta. 1981; 109: 267- 274.
25. Drabkin DL, and Austin JM. Spectrophotometeric consitants for common haemoglobin derivatives in human, dogs and rabbit blood. Journal of Biological Chemistry. 1932; 98:719-33.
26. Singer J M and Plotz C M. The latex fixation test. I. Application to the serologic diagnosis of rheumatoid arthritis. American Journal of Medicine. 1956; 21:888-92.
27. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with the folin phenol reagent. Journal of Biological Chemistry. 1951; 193: 265-275.
28. Zlatkis A, Zak B, Boyle AJ. A new method for the direct determination of serum cholesterol. Journal of Laboratory and Clinical Medicine. 1953; 41: 486.
29. Foster LB, Dunn RT. Stable reagents for determination of serum triglycerides by a colorimetric Hantzsch condensation method. Clinical Chemistry. 1973; 19(3): 338-340.
30. Friedewald WT, Levy RI, Fredrickson DS. Estimation of the concentration of low density lipoprotein cholesterol in plasma, without the use of preparative centrifuge. Clinical Chemistry. 1972; 18(2): 499-502.
31. Girard M, Dreux C. Diaectylmonoxime in the determination of urea. Annales Pharmaceutiques Francaises. 1958; 16: 604.
32. Bonsness RW, Taussky HH. On the colorimetric determination of creatinine by the Jaffe reaction. Journal of Biological Chemistry. 1945; 158: 581-583.
33. Okamoto H, Yamamoto H, Uchigata Y. Streptozotocin and alloxan induce DNA strand breaks and poly (ADP-ribose) synthetase in pancreatic islets. Nature. 1981; 294: 284-6.
34. Marles RJ, Farnsworth NR. Antidiabetic plants and their active constituents. Phytomedicine. 1995; 2(2):137-189.
35. Vessal M, Zal F, Vasi M. Effects of Teucrium polium on oral glucose tolerance test, regeneration of pancreatic islets and activity of hepatic glucokinase in diabetic rats. Archives of Iranian Medicine. 2003; 6: 36-39.
36. Atta-Ur-Rahman, Zaman K. Medicinal plants with hypoglycemic activity. Journal of Ethnopharmacology. 1989; 26: 1-55.
37. Chattopadhyay RR, Sarkar SK, Ganguly S, Banerjee RN, Basu TK. Hypoglycemic and antihyperglycemic effect of leaves of Vinca rosea. Indian Journal of Physiology Pharmacology. 1991; 35(3):145-151.
38. Essawy GS, Sobbhy HM, El-Banna HA. The hypoglycemic effect of volatile oil of some Egyptian plants. Veterinary medical journal Giza. 1995; 43(2):167- 172.
39. Valiathan MS. Healing plants. Current Science. 1998; 75: 1122-1126.
40. Verspohl EJ. Recommended testing in diabetes research. Planta Medica. 2002; 68: 581- 590.
41. Castaneda C. Muscle wasting and protein metabolism. Journal of Animal Sciences. 2002; 80(2): E98-E105.
42. Granner DK. Hormones of the gonads. In: Murray RK, Granner DK, Mayes PA, Rodwell VW, editors. Harper’s Biochemistry, (Stamford, CT: Appleton and Lange Press), 1996, p. 566-580.
43. Pupim LB, Heimburger O, Qureshi AR, Ikizler TA, Stenvinkel P. Accelerated lean body mass loss in incident chronic dialysis patients with diabetes mellitus. Kidney International. 2005; 68: 2368-2374.
44. Iweala EEJ, Obidoa O. Effect of a long term consumption of a diet supplemented with leaves of Gongronema latifolium Benth. On some biochemical and histological parameters in male albino rats. Journal of Biological Sciences. 2009; 9(8): 859-865.
45. Latha M, Pari L. Antihyperglycemic effect of Cassia auriculata in experimental diabetes and its effects on key metabolic enzymes involved in carbohydrate metabolism. Clinical and Experimental Pharmacology and Physiology. 2003; 30: 38-43.
46. Ortiz-Andrade RR, Garcia-Jimenez S, Castillo Espana P, Ramirez-Avila G, Villalobos-Molina R, Estrada-Soto S. Glucosidase inhibitory activity of the methanol extract from Tournefortia hartwegiana: an anti-hyperglycemic agent. Journal of Ethnopharmacology. 2007; 109; 48-53.
47. Alyassin D, Ibrahim KA. Minor haemoglobin fraction and the level of fasting blood glucose. Journal Faculty Medical University of Baghdad. 1981; 23: 373-380.
48. Sheela CG, Augusti KT. Antidiabetic effect of S-allyl cystein sulfoxide from garlic Allium sativum Linn. Indian Journal of Experimental Biology. 1992; 30:523-526.
49. Bunn HG, Gabby KH, Gallop PM. The glycosylation of hemoglobin: relevance to diabetes mellitus. Science. 1978; 200:21-7.
50. Deron Scott J. C - reactive protein: Everything you need to know about it and why it's more important than cholesterol to your health. New York City: McGraw-Hill, 2003.
51. Pradhan AD, Manson JE, Rifai N, Buring JE, Ridker PM. C-reactive protein, interleukin 6, and risk of developing type 2 diabetes mellitus. Journal of the American Medical Association. 2001; 286 (3): 327-34.
52. Dehghan A, Kardys I, de Maat MP, Uitterlinden AG, Sijbrands EJ, Bootama AH, Stijen T, Hofman A, Schram MT Witteman JC. Genetic variation, C-reactive protein levels, and incidence of diabetes. Diabetes. 2007; 56(3): 872-2.
53. Colhoun H M, Schalkwijk C, Rubens M B, Stehouwer C DA. C-reactive protein in type 1 diabetes and its relationship to coronary artery calcification, Diabetes Care. 2002; 25(10): 1813-1817.
54. Mana S, Singhal S, Sharma NK, Singh D. Hypoglycemic effect of Holarrhena antidysenterica seeds on streptozotocin induced diabetic rats. International Journal of PharmTech Research. 2010; 2: 1325-1329.
55. Jones AG, Hattersley AT. The clinical utility of C-peptide measurement in the care of patients with diabetes. Diabetic Medicine. 2013; 30(7):803-17.
56. Clark PM. Assays for insulin, proinsulin and C-peptide. Annals of Clinical Biochemistry. 1999; 36: 541-564.
57. Shapiro ET, Tillil H, Rubenstein AH, Polonsky KS. Peripheral insulin parallels changes in insulin secretion more closely than C-peptide after bolus intravenous glucose administration. Journal of Clinical Endocrinology and Metabolism. 1988; 67(5):1094-9.
58. Chandini R, Udayabhaskaran V, Binoy J Paul, Ramamoorthy KP. A study of non-obese diabetes mellitus in adults in a tertiary care hospital in Kerala, India. International Journal of Diabetes in Developing Countries. 2013; 33 (2): 83-85.
59. Kamalakkannan N, Prince PS. The effect of Aegle marmelos fruit extract in streptozotocin diabetes: A histopathological study. Journal of Herbal Pharmacotherapy. 2005; 5: 87-98.
60. Nair KS, Garrow JS, Ford C, Mahler RF, Halliday D. Effect of poor diabetic control and obesity on whole body protein metabolism in man. Diabetologia. 1983; 25(5):400-3.
61. Charlton MR, Nair KS. Role of hyperglucagonemia in catabolism associated with type 1 diabetes: effects on leucine metabolism and the resting metabolic rate. Diabetes. 1998; 47(11):1748-56.
62. Nair KS, Halliday D, Matthews DE, Welle SL. Hyperglucagonemia during insulin deficiency accelerates protein catabolism. American Journal of Physiology. 1987; 253(2 Pt 1): E208-13.
63. Tessari P, Inchiostro S, Barazzoni R, Zanetti M, Vettore M, Biolo G, Iori E, Kiwanuka E, Tiengo A. Hyperglucagonemia stimulates phenylalanine oxidation in humans. Diabetes. 1996; 45(4):463-70.
64. Vasantha kumara V, Shyamala devi CS. Biochemical evaluation of “Tara Keswara Rasa”- An anti diabetic drug in Rats, Indian Drugs. 1998; 35: 140-143.
65. Wilson JD, Foster DW. William’s textbook of endocrinology (7th edition) Unger RH, Foster DW, editors. Philadelphia: W. B. Saunders Company. 1985.
66. Mauer SM, Steffes MW, Brown DM. The kidney in diabetes. American Journal of Medicine. 1981; 70: 63-6.
67. Almdal TP, Vilstrup H. Strict insulin therapy normalises organ nitrogen contents and the capacity of urea nitrogen synthesis in experimental diabetes in rats. Diabetlogia. 1988; 31: 114-118.
68. Mansour HA, Newairy AA. Amelioration of impaired renal function associated with diabetes by Balanites aegyptiaca fruits in streptozotocin-induced diabetic rats. J Med Res Inst. 2000; 21: 115-25.
69. Al-Attar AM. Physiological effects of some plant oils supplementation on streptozotocin-induced diabetic rats. Research Journal of Medicine in Medical Sciences. 2010; 5: 55-71.
70. Cho SY, Park JY, Park EM. Alteration of hepatic antioxidant enzyme activities and lipid profile in streptozotocin induced diabetic rats by supplementation of dandelion water extract. Clinica Chimica Acta. 2002; 317:109 - 117.
71. Rajalingam R, Srinivasan N, Govindarajulu P. Effect of alloxan induced diabetes on lipid profiles in renal cortex and medulla of mature albino rats. Indian Journal of Experimental Biology. 1993; 31: 577-579.
72. Pathak RM, Ansai S, Mahmood A. Changes in chemical composition of intentional brush border membrane in alloxan induced chronic diabetes. Indian Journal of Experimental Biology. 1981; 19: 503-505.
73. Wang LF, Luo H, Miyoshi M, Imoto T, Hiji Y, Sasaki T. Inhibitory effect of gymnemic acid on intestinal absorption of oleic acid in rats. Canadian Journal of Physiology and Pharmacology. 1998; 76:1017.
74. Loci AS, Shaabha M, Khazraji AL, Husain A, Twaija A. Hypoglycemic effect of a valuable extract of artemicisia herb Alba II. Effect of a valuable extract on some blood parameters in diabetic animals. Journal of Ethnopharmacology. 1994; 43:167-171.
75. Pushparaj PN, Low HK, Manikandan J, Tan BKH, Tan CH. Anti-diabetic effects of Cichorium intybus in streptozotocin-induced diabetic rats. Journal of Ethnopharmacology. 2007; 111: 430-434
76. Shirwaikar A, Rajendran K, Barik R. Effect of aqueous bark extract of Garuga pinnata Roxb. in streptozotocin nicotinamide induced type II diabetes mellitus. Journal of Ethnopharmacology. 2006; 107: 285-290.
77. Levy RI. High-density lipoproteins, an overview, Lipids. 1978; 13: 911-913.
78. Anjaneyulu Muragundla, Chopra Kanwaljit. quercetin, an anti-oxidant bioï¬‚avonoid, attenuates diabetic nephropathy in rats. Clinical and Experimental Pharmacology and Physiology. 2004; 31: 244-8.
79. Salil G, Nevin KG, Rajamohan T. Effect of dietary coconut kernel protein on the liver and pancreas of alloxan-induced diabetic rats: comparison with L-arginine and glibenclamide. Mediterranean Journal of Nutrition and Metabolism. 2012; 5: 127-133.
80. Khushk I, Dahot mo, Baloach SA, Bhutto MA. The evaluation of soybean extracts in alloxan -induced diabetic rabbits. World Applied Sciences Journal. 2010; 8: 22-25.
81. Eskander EF, Jun HW, Ibrahim KA, Abdelal WE. Hypoglycemic effect of an herbal formulation in alloxan induced diabetic rats. Egyptian Journal of Pharmaceutical Sciences. 1995; 36: 253-270.
167 Absract views 165 PDF Downloads 332 Total views
Modulation of some biochemical complications arising from alloxan-induced diabetic conditions in rats treated with Senna occidentalis leaf extractOjochenemi Ejeh Yakubu, Okwesili Fred Chiletugo Nwodo, Chinedu Imo, Sylvester Michael Chukwukadibia Udeh, Mikailu Abdulrahaman, Maryval Ogaku Ogri
Pharmacological evaluation of Prosopis ruscifolia extract on lipid profile in hyperglycemic and hyperlipidemic miceJuan R. Centurión, Elena M. G. Diarte, Antonia Galeano, María S. Soverina, Wilfrido Arrúa, María L. Kennedy, Miguel A. Campuzano-Bublitz
Diversity of insect pollinators of Allium cepa L. (Liliaceae) and assessment of its impact on yields at Gazawa (Cameroon)Georges Tchindebe, Daniel Farda, Dounia, Chantal Douka, Ningatoloum Clautin, Pharaon Mbianda Auguste, Tchuenguem Fohouo Fernand-Nestor
Effect of extraction techniques on anthocyanin from butterfly pea flowers (Clitoria ternatea L.) cultivated in VietnamNguyen Minh Thuy, Tran Chi Ben, Vo Quang Minh, Ngo Van Tai
Efficient plant regeneration and molecular marker-based genetic integrity analysis of Ceropegia lawii Hook: An endemic and endangered herbMukta R. Bhamare, Hemantkumar A. Thakur, Jaykumar J. Chavan
Application of butterfly pea flower extract in processing some Vietnamese traditional foodsNguyen Minh Thuy, Tran Chi Ben, Pham Thi Be Ngoc, Ngo Van Tai,
Morphological, genetic and symbiotic characterization of root nodule bacteria isolated from Bambara groundnuts (Vigna subterranea L. Verdc) from soils of Lake Victoria basin, western KenyaOnyango Benson , Anyango Beatrice, Nyunja Regina, P. K. Koech, Robert A. Skilton , Stomeo Francesca
An efficient micropropagation protocol of Bacopa monnieri (L.) Pennell through two-stage culture of nodal segments and ex vitro acclimatizationShashikanta Behera, Nibedita Nayak, Shasmita, Durga P. Barik, Soumendra K. Naik
Metabolic activities and health indices of African catfish (Clarias gariepinus) fed varying levels of Zingiber officinale rootAdegbesan Sherifat Ibidunni, Obasa Samuel Olubodun, Abdulraheem Ikililu
Rapid in vitro adventitious rooting and proliferation by leaf and nodal cultures of Momordica cymbalaria Fenzl.Chaitanya Gopu, Chandra Shekar Chakilam, Pavani Chirumamilla, Suvarchala Vankudoth, Shasthree Taduri
Biochemical and liver histological changes in rats exposed to sub-lethal dose of Uproot-pesticide and the protective potentials of nutritional supplementsCosmas Onyekachi Ujowundu, Kingsley Isaac Ogamanya, Favour Ntite Ujowundu, Victoria Ojone Adejoh, Calistus I. Iheme, Kalu Okereke Igwe
Impact of chemical properties of soil on spore density, colonization, and distribution of native arbuscular mycorrhizal fungi associated with Capsicum annuum L.Komal Chandrakant Dhumal, Bharat Pandharinath Shinde
Plant induced resistance in Solanacearum lycopersicum species against root knot nematodesB. Navyashree, Chandan Dharmashekar, Chandan Shivamallu, S. Balasubramanian, Shashanka K. Prasad, Shiva Prasad K, K. C. Latha
Cloning the root-specific Asy promoter and genes encoding chitinase 42 kDa of Trichoderma asperellum into the plant expression vectorNguyen Hoang Tue, Tran Gia Cat Tuong, Pham Thi Huyen Trang, Nguyen Duc Chung, Phung Thi Bich Hoa,, Nguyen Quang Duc Tien, Nguyen Hoang Loc
Antioxidant and antibacterial activities of Pandanus amaryllifolius Roxb. (Pandanaceae) prop roots and its application for a novel bacterial cellulose (Nata) fermentation by enzymatic hydrolysisThanasak Lomthong, Manida Chorum, Srisuda Samaimai, Panarat Thongpoem
Management of Meloidogyne incognita and salinity on sweet pepper (Capsicum annuum L.) with different arbuscular mycorrhizal fungus speciesIdorenyin A. Udo, Aniefiok E. Uko, Ekemini E. Obok, Jesam O. Ubi, Sylvia B. A. Umoetok
Accumulation of cadmium in maize roots inoculated with root organ culture of Rhizophagus irregularis improving cadmium tolerance through activation of antioxidative defense enzymesRajamony VinuRadha, Karunanandham Kumutha, Asish Kanagaraj Binodh
Endophytic bacterial metagenomics and phosphate solubilization activities in an endemic legume Humboldtia brunonis Wall.Ganesh V. Shendye, N. Thamizhseran
Effect of liquid seaweed (Ulva rigida) extract on the growth and rooting of carob (Ceratonia siliqua L.)Najat Zouari, Hanane Bougdaoua, Noureddine El Mtili
Agrobacterium rhizogenes as molecular tool for the production of hairy roots in Withania somniferaManali Singh,, Deep Chandra Suyal, Nisha Dinkar, Soniya Joshi, Nishtha Srivastava, Vineet Kumar Maurya, Abhiruchi Agnihotri, Sanjeev Agrawal
Ceiba pentandra (L.) Gaertn.: An overview of its botany, uses, reproductive biology, pharmacological properties, and industrial potentialsEric Wei Chiang Chan,, Siew Wei Yeong, Chen Wai Wong, Oi Yoon Michelle Soo,, Alice Choon Yen Phua, Ying Ki Ng