Research Article | Volume: 4, Issue: 4, July-August, 2016

Modulation of some biochemical complications arising from alloxan-induced diabetic conditions in rats treated with Senna occidentalis leaf extract

Ojochenemi Ejeh Yakubu Okwesili Fred Chiletugo Nwodo Chinedu Imo Sylvester Michael Chukwukadibia Udeh Mikailu Abdulrahaman Maryval Ogaku Ogri   

Open Access   

Published:  Aug 26, 2016

DOI: 10.7324/JABB.2016.40405

The present study was designed to evaluate the effects of Senna occidentalis in alloxan-induced diabetic and its complications in Wistar rats. Thirty male Wistar rats with body weight ranging from 180–250 g were selected for the study. Diabetes was induced by single intraperitoneal dose of alloxan injection (150 mg/ kg body weight). Treatment was carried out orally using aqueous and ethanol extracts of Senna occidentalis leaves at 100 mg/kg body weight once daily for 21-days. The fasting blood sugar (FBS), Thiobarbituric acid Reactive Substance (TBARS), alkaline Phosphatase (ALP), alanine aminotransferase (ALT), aspartate aminotransferase (AST), serum bilirubin and full blood count levels were evaluated. The result of the study showed decrease in FBS, TBARS, liver enzymes, bilirubin, platelets (PLT) and white blood count (WBC) as well as increase in lymphocytes (LYM) and red blood count (RBC). The result of study showed to a large extent the effects of Senna occidentalis in the management of some complications resulting from diabetes and its complications.

Keyword:     Senna occidentalis biochemical complications alloxan-induced diabetes.


Yakubu OE, Nwodo OFC, Udeh SMC, Abdulrahman M., Ogri MO. Modulation of some biochemical complications arising from alloxan-induced diabetic conditions in rats treated with Senna occidentalis leaf extract. J App Biol Biotech. 2016; 4 (04): 051-056. DOI: 10.7324/JABB.2016.40405

Copyright: Author(s). This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.

HTML Full Text

1. Haffner SM, Alexander CM,Cook JJ. Scandinavian Simvastatin Survival Study Group. Reduced Coronary events in simvastatin- treated patients with coronary heart disease and diabetes or impaired fasting glucose levels, sub group analysis from simvastatin survival study. Arch intern Me.1999; 159: 2661-2677.

2. Goldberg RB, Melllies MJ, Sacks FM. Cardiovascular events and their reduction with Pravastatin in diabetic and glucose intolerant myocardial infarction. Survivors with average cholesterol levels: Sub group analysis in the cholesterol and recurrent events. Care investigators. 1998; 98: 2513-2519.

3. Lyons JJ, Jenkins AJ, Zheng D, Lackland DT, McGee D, Gavey WT. Diabetic retinopathy and serum lipoprotein subclasses in DCCT/EDIC Cohort. Invest Opthalmol Vis Sci. 2004; 45(3): 910-918.

4. Tesfaye S, Chaturvedi N, Eaton SE, Ward JD, Manes C, Ionescu-Tirgoviste G, Witte DR, Fuller JH. Vascular risk factors and diabetic neuropathy.N Engl J. Med. 2005; 352(18):1925-1927.

5. McGill HC, McMahan CA, Zieske AW, Sloop GD, Walcott JV, Troxclair DA. Associations of coronary heart disease risk factors with the intermediate lesion of atherosclerosisin youth. The Pathobiological Determinants of Atherosclerosis in Youth (PDAY) Research Group.ArteriosclerThromVasc Biol. 2000;20: 1998-2004.

6. Li TY, Hu FB, Colditz GA, Willet WC, Manson JE. Television watching and other sedentary behaviours in relation to risk of obesity and type 2 diabetes mellitus in women.JAMA.2003; 298(14):1785-1791.

7. Ibrahim SS, Rizk SM. Nicotinamide: a cytoprotectant against streptozotocin-induced diabetic damage in wistar rat brains. Afr. J. Biochem. Res. 2008;2(8):174-180.

8. Sharma SB, Hasir A, Prabhu KM, Murthy PS, Dez G. Hypoglycemic and hypolipidemic effect of ethanolic extract of seeds of Eugenia jambolona in alloxan- induced diabetic rabbits. J Ethnopharmacol. 2003; 85:201-640.

9. Jendrassik L, Gróf P.Vereinfachtephotometrische Methodenzur Bestimmung des Blutbilirubins. Biochem Zeitschrift. 1938; 297:82-9.

10. Feillet-Coudray C, Rock E, Coudray C, Grzelkowska K, Azais-Braesco V, Dardevet D. Lipid peroxidation and anti-oxidant status in experimental diabetes. Clin.Chim.Acta. 1999;284:31-43.

11. Kakkar R, Mantha SV, Radhi J, Prasad K, Kalra J. Antioxidant defence system in diabetic kidney: A time course study. Life Sci.1997;60:667-679.

12. Aydin A, Orhan HA., Sayal M,Ozata G, Isumer A. Oxidative Stress and Nitric Oxide Related Parameters in Type II Diabetes mellitus: Effects of Glycaemic Control. Clin.Biochem.2001;34:65-70.

13. Obresova IG, Fathalah L, Lui E,Nourooz-Zadeh J. Early oxidative stress in diabetic kidney: effect of DL-α-lipoic acid. Free Radic. Biol. Med. 2003;34:186-195.

14. Ugochukwu NH, Babady NE, CobourneMK, Gasset SR. The Effect of GongronemalatifoliumExtracts on Serum Lipid Profile and Oxidative Stress in Hepatocytes of Diabetic Rats. J. Biosci. 2003;28(1):1-5.

15. Sexena DP, Shukla SK, Kumar R. Efficacy studies of In vitro screening of antiplasmodial activity by crude extracts of Diospyrosmelanoxylem plant Res. J. Med. 2011;5:312-320.

16. Ohaeri CC, Eluwa MC. Abnornal biochemical and hematological indices in trypanosomiasis as a threat to herd production. Vet. Parasitol. 2011;177:199-202.

17. U. S. Renal Data System, (USRDS). Annual data report; atlas of end-stage renal disease in the united states, national institutes of health, national institute diabetes and digestive and kidney diseases, Bethesda, MD.2002.

18. Thomas M, Tsalamandris C, MacIsaac R, Jerums G. Anaemia in diabetes: An emerging complication of microvascular disease. Curr.Diabet.Rev. 2005;1:107-126.

19. YoungNS, Maciejewski J.The path physiology of acquired aplastic anemia.New Eng.J. Med. 1997;72336-1365.

20. Nikkila EA. Plasma lipid and lipoprotein abnormalities in diabetes. In: Diabetes and Heart Diseases. Elsevier Science Publishers B.V. Amsterdam.The Netherlands.1984; 134-167.

21. Momoh J, Akoro SM, Godonu KG. Hypoglycemic and Hepatoprotective Effects of Vernoniaamygdalina(Bitter Leaf) and its effect on some biochemical parameters in alloxan-induced diabetic male albino rats.Science Journal of Biochemistry. 2014;194 (10):7237-7244.

Article Metrics
56 Views 38 Downloads 94 Total



Related Search

By author names

Similar Articles