Research Article | Volume 11, Issue 1, January, 2023

Effect of liquid seaweed (Ulva rigida) extract on the growth and rooting of carob (Ceratonia siliqua L.)

Najat Zouari Hanane Bougdaoua Noureddine El Mtili   

Open Access   

Published:  Nov 22, 2022

DOI: 10.7324/JABB.2023.110107

We investigated the in vitro effect of the use of Ulva rigida extract as a plant stimulant on carob root induction. Treatments at different concentrations of U. rigida extract were tested, as well as treatment by immersion of the basal portion of the hypocotylar sections in a solution of indole-3-butyric acid (IBA) before their cultivation in agar medium contains seaweed extract have been tested. The results showed that the application of U. rigida significantly increased the growth and rooting of carob cuttings. Plants treated with U. rigida extract outperformed the untreated plants in terms of plant height, number of internodes, number of leaves, number of roots, and root length with a high percentage of rooting of 83%. The higher response in the adventitious roots of carob hypocotyl cuttings was recorded with a treatment combination with IBA at a concentration of 2 mgL-1 and culturing in a medium containing 4% U. rigida extract. The results demonstrate a significant effect of U. rigida extracts on the root development of Ceratonia siliqua. This study highlights the possibility of developing procedures to improve the rooting capacity of recalcitrant plants while improving their growing environment.

Keyword:     Ulva rigida Ceratonia siliqua Roots In vitro growth Seaweed extract


Zouari N, Bougdaoua H, El Mtili N. Effect of liquid seaweed (Ulva rigida) extract on the growth and rooting of carob (Ceratonia siliqua L.). J App Biol Biotech. 2023;11(1):55-60.

Copyright: Author(s). This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.

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1. Batlle I, Tous J. Carob tree (Ceratonia siliqua L.) Promoting the Conservation and Use of Under-utilized and Neglected Crops. Roma, Gatersleben: Institute of Plant Genetics and Crop Plant Research, International Plant Genetic Resource Institute; 1997.

2. Baumel A, Mirleau P, Viruel J, Bou Dagher KM, La Malfa S, Ouahmane L, et al. Assessment of plant species diversity associated with the carob tree (Ceratonia siliqua, Fabaceae) at the Mediterranean scale. Plant Ecol Evol 2018;151:185-93.

3. Manaut N, Sanguin H, Ouahmane L, Bressan M, Thioulouse J, Baudoin E, et al. Potentialities of ecological engineering strategy based on native arbuscular mycorrhizal community for improving afforestation programs with carob trees in degraded environments. Ecol Eng 2015;79:113-9.

4. Hartmann HD, Kester DE, Davies FJ. Geneve RL Plant Propagation: Principles and Practices. New Jersey: Prentice Hall; 1997.

5. Battacharyya D, Babgohari MZ, Rathor P, Prithiviraj B. Seaweed extracts as biostimulants in horticulture. Sci Hortic 2015;196:39-48.

6. Ali O, Ramsubhag A, Jayaraman J. Biostimulant properties of seaweed extracts in plants: implications towards sustainable crop production. Plants 2021;10:531.

7. Begum M, Bordoloi BC, Singha DD, Ojha NJ. Role of seaweed extract on growth, yield and quality of some agricultural crops: A review. Agric Rev 2018;29:321-6.

8. Chernane H, Latique S, Mansori M, Kaoua ME. Salt stress tolerance and antioxidative mechanisms in wheat plants (Triticum durum L.) by seaweed extracts application. J Agric Vet Sci 2015;8:36-44.

9. Shukla PS, Mantin EG, Adil M, Bajpai S, Critchley AT, Prithiviraj B. Ascophyllum nodosum-based biostimulants: Sustainable applications in agriculture for the stimulation of plant growth, stress tolerance, and disease management. Front Plant Sci 2019;10:655.

10. Jannin L, Arkoun M, Etienne P, Laine P, Goux D, Garnica M, et al. Brassica napus growth is promoted by Ascophyllum nodosum (L.) Le Jol. seaweed extract: Microarray analysis and physiological characterization of N, C, and S metabolisms. J Plant Growth Reg 2013;32:31-52.

11. Mutale-Joan C, Redouane B, Najib E, Yassine K, Lyamlouli K, Laila S, et al. Screening of microalgae liquid extracts for their biostimulant properties on plant growth, nutrient uptake and metabolite profile of Solanum lycopersicum L. Sci Rep 2020;10:2820.

12. Kulkarni MG, Rengasamy KR, Pendota SC, Gruz J, Placková L, Novák O, et al. Bioactive molecules derived from smoke and seaweed ecklonia maxima showing phytohormone-like activity in Spinacia oleracea L. New Biotechnol 2019;48:83-9.

13. Shehata SM, Heba SA, Abou El-Yazied A, El-Gizawy AM. Effect of foliar spraying with amino acids and seaweed extract on growth chemical constitutes, yield and its quality of celeriac plant. Eur J Sci Res 2011;58:257-6.

14. Kapoore RV, Wood EE, Llewellyn CA. Algae biostimulants: A critical look at microalgal biostimulants for sustainable agricultural practices. Biotechnol Adv 2021;49:107754.

15. Anisimov MM, Skriptova AV, Chaikina EL, Klykov AG. Effect of water extracts of seaweeds on the growth of seedling roots of buckwheat. Int J Res Rev Appl Sci 2013;16:282-7.

16. Castellanos BL, Santacruz RF, Hernández CG, Ramírez BE, Hernández HR. Effect of seaweed liquid extracts from Ulva lactuca on seedling growth of mung bean (Vigna radiata). J Appl Phycol 2017;29:2479-88.

17. Valencia RT, Acosta LS, Hernández MF, Rangel PP, Gallegos RM, del Carmen AC, et al. Effect of seaweed aqueous extracts and compost on vegetative growth, yield, and nutraceutical quality of cucumber (Cucumis sativus L.) fruit. Agronomy 2018;8:264.

18. Al-Musawi MA. Effect of foliar application with algae extracts on fruit quality of sour orange, Citrus aurantium L. J Environ Sci Pollut Res 2018;4:250-2.

19. Yusuf R, Kristianse P, Warwick N. Effect of two seaweed products and equivalent mineral treatments on lettuce (Lactuca sativa L.) growth. J Agron 2019;18:100-6.

20. Crouch IJ, van Staden J. Evidence for the presence of plant growth regulators in commercial seaweed products. Plant Growth Reg 1993;6:345-88.

21. Yalç?n S, Sükran OE, Karaka SÖ, Önem AN, Sözgen BK. Identification and quantification of some phytohormones in seaweeds using UPLC-MS/MS. J Liq Chromatogr Relat 2019;42:1-10.

22. Khan W, Rayirath UP, Subramanian S, Jithesh MN, Rayorath P, Hodges DM, et al. Seaweed extracts as biostimulants of plant growth and development. J Plant Growth Reg 2009;28:386-99.

23. Stirk WA, Van Staden J. Plant Growth Regulators in Seaweeds: Occurrence, Regulation and Functions. Amsterdam, Netherlands: Elsevier; 2014. p. 71.

24. Ali O, Ramsubhag A, Jayaraman J. Biostimulatory activities of Ascophyllum nodosum extract in tomato and sweet pepper crops in a tropical environment. PLoS One 2019;14:e0216710.

25. Chen D, Huang Y, Shen D, Zhou W, Ao J, Jiang Y, et al. Effects of seaweed extracts on promoting growth and improving stress resistance in sugarcane. Asian Agric Res 2019;11:69-76.

26. De Klerk GJ, Van der Krieken W, De Jong JC. The formation of adventitious roots: New concepts, new possibilities. In Vitro Cell Dev Biol Plant 1999;35:189-99.

27. De Silva H, McKenzie BA, Bloomberg M. Indolebutyric acid and wounding induced rooting in callused, non-rooted Leyland cypress (9 Cupressocyparis leylandii) stem cuttings. N Zeal J Crop Hortic Sci 2005;33:407-12.

28. Zouari N, El Mtili N. In vitro propagation of mature carob trees (Ceratonia siliqua L.) from the axillary buds. Am J Plant Sci 2020;11:1369-82.

29. Radi A, Echchgadda G, Ibijbijen J, Rochd M. In vitro propagation of Moroccan carob (Ceratonia siliqua L.). J Food Agric Environ 2013;11:1103-7.

30. El Boukhari ME, Barakate M, Bouhia Y, Lyamlouli K. Trends in seaweed extract based biostimulants: Manufacturing process and beneficial effect on soil-plant systems. Plants 2020;9:359.

31. Kurepin LV, Zaman M, Pharis RP. Phytohormonal basis for the plant growth promoting action of naturally occurring biostimulators. J Sci Food Agric 2014;94:1715-22.

32. Arioli T, Mattner SW, Winberg PC. Applications of Seaweed extracts in Australian agriculture: Past, present and future. J Appl Phycol 2015;27:2007-15.

33. Wang Y, Fu F, Li J, Wang G, Wu M, Zhan J, et al. Effects of Seaweed fertilizer on the growth of malus hupehensis rehd. Seedlings, soil enzyme activities and fungal communities under replant condition. Mattner SW, Milinkovic M, Arioli T. Increased growth response of strawberry roots to a commercial extract from durvillaea potatorum and Ascophyllum nodosum. J Appl Phycol 2018;30:2943-51.

35. Carmody N, Goñi O, ?angowski ?, O'Connell S. Ascophyllum nodosum extract Biostimulant processing and its impact on enhancing heat stress tolerance during tomato fruit set. Front Plant Sci 2020;11:807.

36. Zouari N, El Mtili N. Ultrasonic influence on carob tree (Ceratonia siliqua L.) rooting under in-vitro conditions. Moroccan J Biol 2018;15:18-27.

37. Zouari N, El Mtili N. Effects of ectomycorrhizal fungal inoculation on growth and rooting of carob tree (Ceratonia siliqua L.). South Afr J Bot 2020;135:181-7.

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