Research Article | Volume 10, Issue 4, July, 2022

Antifungal effects of Kurthia gibsonii Mb 126 chitinase as a seed treatment on seed-borne fungi of rice seed on germination percentage and seedling vigor

Mini K. Paul K. D. Mini Jyothis Mathew   

Open Access   

Published:  Jun 01, 2022

DOI: 10.7324/JABB.2022.100417

Soil and seed-borne phytopathogenic fungi are the main factors limiting crop yield in India’s agricultural sector. They attack the root of the seed before germination or seedling after germination resulting in huge deprivation in crop yield. In this scenario, it is crucial to control phytopathogenic fungi to ensure sustainable food production to the ever-increasing world population. The antifungal property of purified chitinase of Kurthia gibsonii Mb 126 was investigated by isolating fungi infected with seeds of various rice samples and then studying the effect of purified chitinase of K. gibsonii Mb 126 on these isolated fungi. The effect of K. gibsonii Mb 126 purified chitinase on the germination of rice seed infested with these isolated fungi was also investigated. Eight fungi (Aspergillus niger, Aspergillus flavus, Curvularia lunata, Fusarium moniliforme, Rhizopus oryzae, Trichoderma harzianum, Rhizoctonia solani, and Fusarium subglutinans) were isolated and identified from the different rice varieties of Kerala, India, viz Aswathy (PTB 37), Jaya, Sabari (PTB 40), Ahalya, Onam, Makam, Triveni (PTB 38), Swarnaprabha, Kairali, Pavizham, and Ponni, The frequency of isolated fungi ranged from 46% to 100% (present in all the 20 samples). The isolated fungi C. lunata, A. flavus, R. solani, and A. niger were predominated. Seeds treated with the K. gibsonii Mb 126 chitinase enzyme had a strong germination response. K. gibsonii Mb 126 chitinase proved beneficial in eliminating seed-borne fungus, boosting seed germination percentage, and seedling vigor. Farmers of our country should be aware of soil and seed-borne fungi, and they should do seed treatment with chitinase before sowing in the agricultural field, and through this strategy, they will be more benefited.

Keyword:     Kurthia gibsonii Mb126 chitinase Antifungal Rice seed germination Seedling vigor


Paul MK, Mini KD, Mathew J. Antifungal effects of Kurthia gibsonii Mb 126 chitinase as a seed treatment on seed-borne fungi of rice seed on germination percentage and seedling vigor. J App Biol Biotech. 2022;10(4):130-135. DOI: 10.7324/JABB.2022.100417

Copyright: Author(s). This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.

HTML Full Text

1. Ganesh RN, Jayalakshmi K, Naik TB. Efficacy of fungicides on the management of sheath blight of rice. Int J Curr Microbiol Appl Sci 2017;6:611-4.

2. Islam MS, Rahman H, Pervez Z, Mahmud MR, Alam A. Studies on seed-borne fungi in rice cultivars grown in non-saline tidal zones of Patuakhali and their effect on seed germination. Bangladesh Res Publ J 2012;6:286-90.

3. Neergaard P. Seed Pathology. New Delhi: S. Chand and Company Ltd.; 1986.

4. Collinge DB, Kragh KM, Mikkelsen JD, Nielsen KK, Rasmussen U, Vad K. Plant chitinases. Plant J 1993;3:31-40.

5. Chernin, L, Ismailov Z, Haran S, Chet I. Chitinolytic Enterobacter agglomerans antagonistic to fungal plant pathogens. Appl Environ Microbiol 1995;61:1720-6.

6. Chang WT, Chen CS, Wang SL. An antifungal chitinase produced by Bacillus cereus with shrimp and crab shell powder as carbon source. Curr Microbiol 2003;47:102-8.

7. Freeman S, Minzm O, Kolesnik I, Barbul O, Zveibil A, Maymon M, et al. Trichoderma biocontrol of Colletotrichum acutatum and Botrytis cinerea and survival in strawberry. Eur J Plant Pathol 2004;110:361-70.

8. Mathivanan N, Kabilan V, Murugesan K. Purification, characterization and antifungal activity of chitinase from Fusarium chlamydosporum, a mycoparasite to groundnut rust, Puccinia arachidis. Can J Microbiol 1998;44:646-51.

9. Miller GL. Use of dinitrosalicylic acid reagent for determination of reducing sugars. Anal Chem 1959;31:426-8.

10. De Hoog GS, Guarro J, Gene J, Figueras MJ. Atlas of Clinical Fungi. 2nd ed. The Netherlands: Centraal Bureau Voor Schimmelcultures Utrecht; 2000.

11. Nghiep VH, Gaur A. Efficacy of seed treatment in improving seed quality in rice. Omonrice 2005;13:42-51.

12. Uniformity in seed quality evaluation worldwide. International seed testing association. Seed Sci Technol 1985;13:307-513.

13. Mia MA, Rahman M, Pearce D, Holderness M. Effect of seed-borne Biplaris oryzae on seed germination and disease development in the field. Bangladesh J Plant Pathol 2002;17:59-62.

14. Khan MA, Ahmad K, Ahmad J. Effect of potassium levels on the yield of sunflower (Helianthus annuus L.). Pak J Biol Sci 1999;2:402-3.

15. Khan TZ, Gill MA, Khan MG. Seed-borne fungi of rice from central Punjab and their control. Pak J Phytopathol 2000;12:12-4.

16. Wahid A, Saleem M, Khan MU, Tariq H, Saleem A. Seed-borne mycoflora of rice. J Agric Res 1993;31:95-100.

17. Javaid MS, Wahid A, Idrees M, Gill MA, Saleem A. Seed mycoflora studies in rice. Pak J Phytopathol 2002;14:132-4.

18. Brooks S. Sensitivity to a Phytotoxin from Rhizoctonia solani correlates with sheath blight susceptibility in rice. Phytopathology 2007;97:1207-12.

19. Baker R. Trichoderma spp. as plant-growth stimulants. Biotechnology 1988;7:97-106.

20. Woodstock LW. Proceedings of the International Seed Technology Association (ISTA); 1969. p. 273-80.

21. Reyes-Ramirez A, Escudero-Abarca BI, Aguilar-Uscanga G, Hayward-Jones PM, Corona JE. Antifungal activity of Bacillus thuringiensis chitinase and its potential for the biocontrol of phytopathogenic fungi in soybean seeds. J Food Sci 2004;69:M131-4.

Article Metrics
39 Views 31 Downloads 70 Total



Related Search

By author names

Similar Articles

Study of endophytic Bacillus amyloliquefaciens CC09 and its antifungal cyclic lipopeptides

Cai Xun-Chao,Li Hui,Xue Ya-Rong,Liu Chang-Hong

Determination of phytochemical, antioxidant, antimicrobial, and protein binding qualities of hydroethanolic extract of Celastrus paniculatus

Vijay Kumar¥, Simranjeet Singh¥, Arjun Singh¥, Amit Kumar Dixit¥, Bhavana Shrivastava, Sapna Avinash Kondalkar, Joginder Singh, Ravindra Singh, Gurpreet Kaur Sidhu, Rajesh Partap Singh, Varanasi Subhose, Om Prakash

Phytochemical analysis, antimicrobial and antioxidant activities of Aidia borneensis leaf extracts

Zulhamizan Awang-Jamil, Aida Maryam Basri, Norhayati Ahmad, Hussein Taha

Role of medicinal plants in the treatment of eumycetoma: A review

Shashank M. Patil, S. Jagadeep Chandra, M. K. Jayanthi, Prithvi S. Shirahatti, Ramith Ramu

Use of the amphotericin B, miconazole, and sodium hypochlorite to control the growth of the robust Aspergillus flavus and Aspergillus fumigatus biofilms on polyethylene support

Camila Guedes Francisco, Gilberto Bida Leite Braga, Luis Henrique Souza Guimarães

Investigation on the antifungal activity of Aspergillus giganteus in different culture conditions

S. Karthiga, R. Ramya, K. Ramya, S. Jothinayaki, D. Kavitha

Evaluation of the antifungal effect of medicinal plants against Panama wilt of Banana caused by Fusarium oxysporum f. sp. cubense

Basavanapura Linganna Kiran,, Kallahally Nagaraj Nayana, Koteshwar Anandrao Raveesha,

Silver nanoparticles decorated natural products doped polyaniline hybrid materials for biomedical applications

K. Satish, K. Sumangala Bhat, Y. S. Ravikumar, M. N. K. Harish

Surveillance of upper aerodigestive candidiasis and their antifungal susceptibility study at tertiary care hospital: A prospective study

Priyanka Debta, Santosh Kumar Swain, Debasmita Dubey, Smrutipragnya Samal, Fakir Mohan Debta, Smarita Lenka

Green synthetic photo-irradiated chitin-silver nanoparticles for antimicrobial applications

Navya Kumari Tenkayala,, Laxman Vamshi Krishna Kandala, Roopkumar Sangubotla, Rambabu Gundla, Subramani Devaraju