Plant growth-promoting Pseudomonas spp. from jasmine rhizosphere as a sustainable alternative to agrochemicals
The global jasmine flower market has grown significantly over the decades, often relying heavily on synthetic agrochemicals to meet the increasing demand. However, growing concerns about the environmental impact of chemical-based floriculture have shifted attention towards green alternatives. Microbial plant growth promoters (PGP) offer promising bio-based and safe solutions. The present study focused on six PGP isolates belonging to the genus Pseudomonas, isolated from the rhizosphere soils of the geographical indication tagged Udupi Jasmine cultivated in Karnataka, India. This study involved isolation and functional characterization, followed by evaluation using bioassays and pot culture experiments. The isolates exhibited multiple beneficial traits. The green gram seeds treated with strains NPS-18 and NPS-6 exhibited 100% germination compared to only 6.66 ± 0.28% in the untreated control after 17 h. All isolates produced key phytohormones, including indole acetic acid and gibberellic acid, improving plant growth indices. The pot culture experiment showed that isolate NPS-18 was the most promising strain in terms of growth enhancement. This strain was identified as Pseudomonas aeruginosa using 16S rRNA gene sequencing. Overall, the versatile functional properties of the native PGP strains demonstrate their potential for use in sustainable jasmine cultivation.
Mundala RR, Badkillaya RR. Plant growth-promoting Pseudomonas spp. from jasmine rhizosphere as a sustainable alternative to agrochemicals. J Appl Biol Biotech 2025. Article in Press. http://doi.org/10.7324/JABB.2025.252029
1. Anumala NV, Kumar R. Floriculture sector in India: Current status and export potential. J Hortic Sci Biotechnol. 2021;96(5):673-80. https://doi.org/10.1080/14620316.2021.1902863
2. Lu Z, Wang X, Lin X, Mostafa S, Bao H, Ren S, et al. Genome-wide identification and characterization of long non-coding RNAs associated with floral scent formation in Jasmine (Jasminum sambac). Biomolecules. 2023;14(1):45. https://doi.org/10.3390/biom14010045
3. Chaitanya HS, Nataraja S, Vikram HC, Jayalakshmi NH. Review on production techniques of GI Crop, Udupi Mallige (Jasminum sambac (L.) Aiton). J Pharmacogn Phytochem. 2018;3:50-2.
4. Handy F, Cnaan RA, Bhat G, Meijs LC. Jasmine growers of coastal Karnataka: Grassroots sustainable community-based enterprise in India. Entrep Reg Dev. 2011;23(5-6):405-17. https://doi.org/10.1080/08985626.2011.580166
5. Vacheron J, Desbrosses G, Bouffaud ML, Touraine B, Moënne- Loccoz Y, Muller D, et al. Plant growth-promoting rhizobacteria and root system functioning. Front Plant Sci 2013;4:356. https://doi.org/10.3389/fpls.2013.00356
6. Zboralski A, Filion M. Pseudomonas spp. can help plants face climate change. Front Microbiol. 2023;14:1198131. https://doi.org/10.3389/fmicb.2023.1198131
7. Beneduzi A, Ambrosini A, Passaglia LMP. Plant growth-promoting rhizobacteria (PGPR): Their potential as antagonists and biocontrol agents. Genet Mol Biol 2012;35(4 Suppl):1044-51. https://doi.org/10.1590/s1415-47572012000600020
8. Zhang H, Zheng D, Hu C, Cheng W, Lei P, Xu H, et al. Certain tomato root exudates induced by Pseudomonas stutzeri NRCB010 enhance its rhizosphere colonization capability. Metabolites. 2023;13(5):664. https://doi.org/10.3390/metabo13050664
9. Singh P, Singh RK, Zhou Y, Wang J, Jiang Y, Shen N, et al. Unlocking the strength of plant growth promoting Pseudomonas in improving crop productivity in normal and challenging environments: A review. J Plant Interact. 2022;17(1):220-38. https://doi.org/10.1080/17429145.2022.2029963
10. King EO, Ward MK, Raney DE. Two simple media for the demonstration of pyocyanin and fluorescin. Transl Res. 1954;44(2):301-7.
11. DeBritto ST, Gajbar D, Satapute P, Sundaram L, Lakshmikantha RY, Jogaiah S, et al. Isolation and characterization of nutrient dependent pyocyanin from Pseudomonas aeruginosa and its dye and agrochemical properties. Sci Rep. 2020;10(1):1-12. https://doi.org/10.1038/s41598-020-58335-6
12. Aneja KR. Experiments in Microbiology, Plant Pathology and Biotechnology. 6th ed. New Delhi: New Age International; 2022.
13. Ramya Rai M, Rao RB. Isolation of phosphate solubilizing microorganisms from geographical indication tagged Shankarpura jasmine [Jasminum sambac (L.) Aiton] and their plausible role in plant growth promotion. Bioscene. 2024;21(3):856-74.
14. Kotasthane AS, Agrawal T, Zaidi NW, Singh US. Identification of siderophore producing and cyanogenic fluorescent Pseudomonas and a simple confrontation assay to identify potential biocontrol agent for collar rot of chickpea. 3 Biotech. 2017;7(2):137. https://doi.org/10.1007/s13205-017-0761-2
15. Krishnaraj PU. Genetic Characterization of Mineral Phosphate Solubilization in Pseudomonas sp. [Ph.D. Thesis]. New Delhi: Indian Institute of Agricultural Sciences; 1996.
16. Gang S, Sharma S, Saraf M, Buck J, Schumacher J. Production in Klebsiella by LC-MS/MS and the Salkowski method. Bio Protoc. 2019;9(9):3230. https://doi.org/10.21769/bioprotoc.3230
17. Berríos J, Illanes A, Aroca G. Spectrophotometric method for determining gibberellic acid in fermentation broths. Biotechnol Lett. 2004;26:67-70.
18. Nathan Vinod Kumar K, Rajam S, Rani ME. Plant growth promotion efficacy of indole acetic acid (IAA) produced by a mangrove associated fungi Trichoderma viride VKF3. Int J Curr Microbiol Appl Sci. 2017;6(11):2692-701.
19. Bhagobaty RK, Joshi SR. Promotion of seed germination of green gram and chickpea by Penicillium verruculosum RS7PF, a root endophytic fungus of Potentilla fulgens L. Adv Biotech. 2009;8:16-8.
20. Dereeper A, Guignon V, Blanc G, Audic S, Buffet S, Chevenet F, et al. Phylogeny.fr: Robust phylogenetic analysis for the non-specialist. Nucleic Acids Res. 2008;36:gkn180. https://doi.org/10.1093/nar/gkn180
21. Gopinath PP, Prasad R, Joseph B, Adarsh VS. Collection of shiny apps for data analysis in agriculture grapesAgri1. J Open Source Softw. 2021;6(63):3437. https://doi.org/10.21105/joss.03437
22. Sah S, Krishnani S, Singh R. Pseudomonas mediated nutritional and growth promotional activities for sustainable food security. Curr Res Microb Sci. 2021;2:100084. https://doi.org/10.1016/j.crmicr.2021.100084
23. Gutierrez-Albanchez E, García-Villaraco AJ, Lucas I, Horche B, Ramos-Solano E, Gutierrez-Mañero FJ. Pseudomonas palmensis sp. nov., a novel bacterium isolated from Nicotiana glauca microbiome: Draft genome analysis and biological potential for agriculture. Front Microbiol 2021;12:672751. https://doi.org/10.3389/fmicb.2021.672751
24. Domínguez-Bello MG, Reyes N, Teppa-Garrán A, Romero R. Interference of Pseudomonas strains in the identification of Helicobacter pylori. J Clin Microbiol. 2000;38(2):937. https://doi.org/10.1128/jcm.38.2.937-937.2000
25. Dodd CER. Pseudomonas - introduction. In: Batt CA, Tortorello ML, editors. Encyclopedia of Food Microbiology. 2nd ed. New York: Elsevier; 2014. p. 244-7.
26. Ou K, He X, Cai K, Zhao W, Jiang X, Ai W, et al. Phosphate-solubilizing Pseudomonas sp. strain WS32 rhizosphere colonization-induced expression changes in wheat roots. Front Microbiol. 2022;13:927889. https://doi.org/10.3389/fmicb.2022.927889
27. Yadav AN. Phosphate-solubilizing microorganisms for agricultural sustainability. J Appl Biol Biotechnol. 2022;10(3):1-6. https://doi.org/10.7324/jabb.2022.103ed
28. Bakki M, Banane B, Marhane O, Esmaeel Q, Hatimi A, Barka EA, et al. Phosphate solubilizing Pseudomonas and Bacillus combined with rock phosphates promoting tomato growth and reducing bacterial canker disease. Front Microbiol. 2024;15:1289466. https://doi.org/10.3389/fmicb.2024.1289466
29. Orozco-Mosqueda MD, Santoyo G, Glick BR. Recent advances in the bacterial phytohormone modulation of plant growth. Plants. 2023;12(3):606. https://doi.org/10.3390/plants12030606
30. Zhang T, Jian Q, Yao X, Guan L, Li L, Liu F, et al. Plant growth-promoting rhizobacteria (PGPR) improve the growth and quality of several crops. Heliyon. 2024;10(10):e31553.
31. Khoso MA, Wagan S, Alam I, Hussain A, Ali Q, Saha S, et al. Impact of plant growth-promoting rhizobacteria (PGPR) on plant nutrition and root characteristics: Current perspective. Plant Stress. 2024;11:100341. https://doi.org/10.1016/j.stress.2023.100341
32. Singh TB, Sahai V, Ali A, Prasad M, Yadav A, Shrivastav P, et al. Screening and evaluation of PGPR strains having multiple PGP traits from the hilly terrain. J Appl Biol Biotech. 2020;8(4):38-44. https://doi.org/10.7324/jabb.2020.80406
33. Sharma S, Sharma A, Kaur M. Extraction and evaluation of gibberellic acid from Pseudomonas sp.: plant growth promoting rhizobacteria. J Pharmacogn Phytochem. 2018;7(1):2790-5.
34. Lata DL, Abdie O, Rezene Y. IAA-producing bacteria from the rhizosphere of chickpea (Cicer arietinum L.): Isolation, characterization, and their effects on plant growth performance. Heliyon. 2024;10(21):e39702. https://doi.org/10.1016/j.heliyon.2024.e39702
35. Hasan A, Tabassum B, Hashim M, Khan N. Role of plant growth promoting rhizobacteria (PGPR) as a plant growth enhancer for sustainable agriculture: A review. Bacteria. 2024;3(2):59-75. https://doi.org/10.3390/bacteria3020005
36. Alybayev S, Smekenov I, Kuanbay A, Sarbassov D, Bissenbaev A. Gibberellic-acid-dependent expression of α-amylase in wheat aleurone cells is mediated by target of rapamycin (TOR) signaling. Curr Plant Biol. 2024;37:100312. https://doi.org/10.1016/j. cpb.2023.100312
37. Kaneko M, Itoh H, Ueguchi-Tanaka M, Ashikari M, Matsuoka M. The alpha-amylase induction in endosperm during rice seed germination is caused by gibberellin synthesized in epithelium. Plant Physiol. 2002;128(4):1264-70. https://doi.org/10.1104/pp.010785
38. Meliani A, Bensoltane A, Benidire L, Oufdou K. Plant growth promotion and IAA secretion with Pseudomonas fluorescens and Pseudomonas putida. Res Rev J Bot Sci. 2017;6(2):16-24.
39. Tsukanova KA, Chebotar VK, Meyer JJ, Bibikova TN. Effect of plant growth-promoting rhizobacteria on plant hormone homeostasis. S Afr J Bot. 2017;113:91-102. https://doi.org/10.1016/j.sajb.2017.07.007
40. Fatima T, Arora NK. Pseudomonas entomophila PE3 and its exopolysaccharides as biostimulants for enhancing growth, yield and tolerance responses of sunflower under saline conditions. Microbiol Res. 2021;244:126671. https://doi.org/10.1016/j.micres.2020.126671
41. Xie H, Pasternak JJ, Glick BR. Isolation and characterization of mutants of the plant growth-promoting rhizobacterium Pseudomonas putida GR12-2 that overproduce indoleacetic acid. Curr Microbiol. 1996;32:67-71. https://doi.org/10.1007/s002849900012
42. Gupta R, Chakrabarty SK. Gibberellic acid in plant: Still a mystery unresolved. Plant Signal Behav. 2013;8(9):e25504. https://doi.org/10.4161/psb.25504
43. Qessaoui R, Bouharroud R, Furze JN, El Aalaoui M, Akroud H, Amarraque A, et al. Applications of new rhizobacteria Pseudomonas isolates in agroecology via fundamental processes complementing plant growth. Sci Rep. 2019;9:12832. https://doi.org/10.1038/s41598-019-49216-8
44. Jakubowska Z, Gradowski M, Dobrzy?ski J. Role of plant growth-promoting bacteria (PGPB) in enhancing phenolic compounds biosynthesis and its relevance to abiotic stress tolerance in plants: A review. Antonie van Leeuwenhoek. 2025;118(9):123. https://doi.org/10.1007/s10482-025-02130-8
45. Zhang S, Deng Z, Borham A, Ma Y, Wang Y, Hu J, et al. Significance of soil siderophore-producing bacteria in evaluation and elevation of crop yield. Horticulturae. 2023;9(3):370. https://doi.org/10.3390/horticulturae9030370
46. Bhavyashree P, Rao RB, Bhat P. A study on plant growth promotional and antagonistic properties of lactic acid bacteria. J Adv Sci Res. 2021;2020(CSTSS):1-10. Available from: https://sciensage.info/index.php/JASR/article/view/1637
47. Ashritha, Rao RB, Rai MR, Nagaraj P, Visweswara P. Characterization of phosphate solubilising bacteria isolated from rhizosphere soils of Piper nigrum L. Biotechnol. 2021;20(1):15-21. https://doi.org/10.3923/biotech.2021.15.21
48. Ramya Rai M, Rao RB. Identification of native Trichoderma for augmentation of plant growth in geographical indication tagged fragrant Shankarpura jasmine [Jasminum sambac (L.) Aiton]. J Biol Control. 2024;38(4):389-97.
49. Zameer F, Meghashri S, Gopal S, Rao BR. Chemical and microbial dynamics during composting of herbal pharmaceutical industrial waste. J Chem. 2010;7(1):143-8. https://doi.org/10.1155/2010/645978
50. Kavya H, Akki AJ, Rao RB, Goveas SW, Sridhar KR. Plant growth-promotion and antipathogenic fungal activity of four rhizosphere isolates of Trichoderma from southwest India. Plant Fungal Res 2025;8(1):6. https://doi.org/10.30546/2664-5297.2025.8.1.06
51. Chopra A, Bobate S, Rahi P, Banpurkar A, Mazumder PB, Satpute S. Pseudomonas aeruginosa RTE4: A tea rhizobacterium with potential for plant growth promotion and biosurfactant production. Front Bioeng Biotechnol. 2020;8:861. https://doi.org/10.3389/fbioe.2020.00861
52. Ghadamgahi F, Tarighi S, Taheri P, Saripella GV, Anzalone A, Kalyandurg PB, et al. Plant growth-promoting activity of Pseudomonas aeruginosa FG106 and its ability to act as a biocontrol agent against potato, tomato and taro pathogens. Biology. 2022;11(1):140. https://doi.org/10.3390/biology11010140
53. Krishnan Kutty S, Skandasamy N, Padma Devi R, Djearamane S, Tey LH, Wong LS, et al. Field trial to correlate mineral solubilization activity of Pseudomonas aeruginosa and biochemical content of groundnut plants. Open Life Sci. 2025;20:20221008. https://doi.org/10.1515/biol-2022-1008
54. Katiyar P, Pandey N, Varghese B, Sahu KK. Biopriming of Pseudomonas aeruginosa abates fluoride toxicity in Oryza sativa L. by restricting fluoride accumulation, enhancing antioxidative system, and boosting activities of rhizospheric enzymes. Plants. 2025;14(8):1223. https://doi.org/10.3390/plants14081223
55. Thakker JN, Rathod K, Patel K, Pandya J, Badrakia J, Dhandhukia P. Blue-green partnership: Unravelling potential of marine Pseudomonas aeruginosa OG as a biocontrol agent against Fusarium oxysporum f. sp. vasinfectum. Discov Oceans. 2025;2(1):13. https://doi.org/10.1007/s44289-025-00052-x
56. ICAR-National Bureau of Agriculturally Important Microorganisms (NBAIM). Technologies for Sustainable Agriculture: Compilation of Technologies Developed by ICAR-NBAIM. Mau: ICAR-NBAIM; 2022. Available from: https://icar.org.in/sites/default/files/2022-06/technologies-compilation_nbaim.pdf
57. Zameer F, Rukmangada MS, Chauhan JB, Khanum SA, Kumar P, Devi AT, et al. Evaluation of adhesive and anti-adhesive properties of Pseudomonas aeruginosa biofilms and their inhibition by herbal plants. Iran J Microbiol. 2016;8(2):108-19.
58. Avinash MG, Aishwarya S, Zameer F, Gopal S. Pseudomonas aeruginosa bio-film and their molecular escape strategies. J App Biol Biotech. 2023;11(3):28-37. https://doi.org/10.7324/jabb.2023.36700
59. Morganal, Diggle SP, Whiteley M. Pseudomonas aeruginosa: Ecology, evolution, pathogenesis and antimicrobial susceptibility. Nat Rev Microbiol. 2025; Advance online publication. https://doi.org/10.1038/s41579-025-01193-8
60. Vidal-Cortés P, Campos-Fernández S, Cuenca-Fito E, Del Río- Carbajo L, Fernández-Ugidos P, López-Ciudad VJ, et al. Difficult-to-Treat Pseudomonas aeruginosa infections in critically Ill patients: A comprehensive review and treatment proposal. Antibiotics. 2025;14(2):178. https://doi.org/10.3390/antibiotics14020178
61. Vasco G, Martínez R, Noboa D, Vasco K, Trueba G. Physiological adaptations of clinical vs. indoor environmental strains of Pseudomonas aeruginosa in a hospital setting. FEMS Microbiol Lett 2025;372:fnaf027. https://doi.org/10.1093/femsle/fnaf027
62. Gao J, Zhuang S, Zhang W. Advances in plant auxin biology: Synthesis, metabolism, signaling, interaction with other hormones, and roles under abiotic stress. Plants. 2024;13(17):2523. https://doi.org/10.3390/plants13172523
63. Chebotar GO, Chebotar SV. Gibberellin-signaling pathways in plants. Cytol Genet. 2011;45(4):259-68. https://doi.org/10.3103/s0095452711040037
64. Singh P, Chauhan PK, Upadhyay SK, Singh RK, Dwivedi P, Wang J, et al. Mechanistic insights and potential use of side rophores producing microbes in rhizosphere for mitigation of stress in plants grown in degraded land. Front Microbiol. 2022;13:898979. https://doi.org/10.3389/fmicb.2022.898979
Year
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