Gibberellic acid treatment improves seed germination and seedling establishment in Tinospora cordifolia (Willd.) Hook. F. and Thoms

Moitri Bhadra Sumita Mondal Adwaita Das Abhijit Bandyopadhyay   

Open Access   

Published:  Jan 31, 2024

DOI: 10.7324/JABB.2024.158607

Tinospora cordifolia is a well-known medicinally important deciduous woody climber plant of Indian subcontinent. The seeds of T. cordifolia are plagued by poor germination capacity due to short duration of viability. Untreated 7-day-old seeds show 83.33% germination. However, a gradual decrease in germination is noticed up to 13.33% within 3 months. This problem raises an obstacle for sustainable use of these seeds. A simple manipulation of germination protocol is standardized to overcome this problem. In this present experiment, gibberellic acid (GA3) and potassium nitrate have been used in different concentrations (100, 300, and 500 ppm) under 16 h/8 h day and night regime at room temperature. The effect of treatment was analyzed by assessing standard germination parameters, seedling growth parameters, vigor index, and biochemical changes in both treated and untreated seeds. The experimental findings suggest that 3-month-old seeds with GA3 treatment recorded 100% germination. Most of our germination responses display the best result at a higher concentration of GA3 (300 and 500 ppm) compared to other experimental conditions. Thus, it can be concluded that the GA3 at 300 ppm has a tremendous promotional effect on the seed germination of T. cordifolia and thus recommended for effective handling of tackling poor seed germinability.

Keyword:     Tinospora cordifolia Medicinal plant Seed germination Gibberellic acid Potassium nitrate


Bhadra M, Mondal S, Das A, Bandyopadhyay A. Gibberellic acid treatment improves seed germination and seedling establishment in Tinospora cordifolia (Willd.) Hook. F. and Thoms. J App Biol Biotech. 2024. Online First. DOI:

Copyright: Author(s). This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.

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