Comparative biochemical composition and exploratory UPLC–ESI–QTOF–MSE metabolite profiling of marine macrophytes from the Mekong Delta Coast, Vietnam

Huu-Tri Nguyen; Huynh-Mai Do; Phuong-Loan Phan; Huu-Thanh Nguyen; Phu-Tho Nguyen

doi:10.7324/jabb.2026.324961

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Abstract

Marine macrophytes are promising coastal bioresources, yet their biochemical composition and metabolite profiles along Vietnam’s Mekong Delta coast remain poorly characterized. This study compared the proximate composition, phenolic-related compounds, photosynthetic pigments, and ultraperformance liquid chromatography coupled with electrospray ionization quadrupole time-of-flight mass spectrometry operated in MS? acquisition mode (UPLC–ESI– QTOF–MS?)-based metabolite profiles of Sargassum sp., Padina sp., Turbinaria ornata, and Enhalus acoroides. Taxonomic assignments were supported by partial chloroplast 23S rRNA gene sequencing, and the samples were analyzed for moisture, crude protein, n-hexane-extractable lipid, total ash, total polyphenol, total flavonoid, total chlorophyll, and total carotenoid contents. Ethanolic extracts were further examined by exploratory UPLC–ESI– QTOF–MS? screening. Sargassum sp. showed the highest crude protein content (23.22% DW), although it was not significantly different from Padina sp. (22.19% DW). Padina sp. exhibited the highest total polyphenol (18.48 mg GAE/g DW), total flavonoid (11.21 mg CE/g DW), and total chlorophyll contents (3.31 mg/g DW). Under the applied screening conditions, the pooled Padina sp. extract yielded the highest number of tentatively assigned molecular features, mainly related to fatty acids, terpenoids, organic acids, sugar alcohols, and phenolics. These findings provide preliminary biochemical baseline data for local marine macrophytes and support further replicated, targeted analyses to confirm the molecular features and evaluate their potential applications.

Keyword: Brown seaweed marine macrophytes Mekong Delta pigments polyphenols UPLC–ESI–QTOF–MS? profiling

Citation:

Nguyen H-T, Do H-M, Phan P-L, Nguyen H-T, Nguyen P-T. Comparative biochemical composition and exploratory UPLC–ESI–QTOF–MS? metabolite profiling of marine macrophytes from the Mekong Delta Coast, Vietnam. J Appl Biol Biotech 2026. Article in Press. http://doi.org/10.7324/jabb.2026.324961

Copyright: Author(s). This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.

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1. Do Amaral Camara Lima M, Bergamo TF, Ward RD, Joyce CB. A review of seagrass ecosystem services: providing nature-based solutions for a changing world. Hydrobiologia 2023;850(12):2655–70; doi: https://doi.org/10.1007/s10750-023-05244-0

2. Xie C, Lee ZJ, Ye S, Barrow CJ, Dunshea FR, Suleria HAR. A review on seaweeds and seaweed-derived polysaccharides: nutrition, chemistry, bioactivities, and applications. Food Rev Int 2024;40(5):1312–47; doi: https://doi.org/10.1080/87559129.2023.2212055

3. Ward M, Dibble C, Millington-Drake M, Lynch J, Mcglathery K, Lilley RJ, et al. Management approach matters: meeting seagrass recovery and carbon mitigation goals. NPJ Ocean Sustain 2025;4(1):18; doi: https://doi.org/10.1038/s44183-025-00111-y

4. Cadar E, Popescu A, Dragan AML, Pesterau AM, Pascale C, Anuta V, et al. Bioactive compounds of marine algae and their potential health and nutraceutical applications: a review. Mar Drugs 2025;23(4):152; doi: https://doi.org/10.3390/md23040152

5. Can B, Sanlier N. Secret heroes of the sea: brown macroalgae and their bioactive powers—a narrative review. Front Nutr 2026;13:1766041; doi: https://doi.org/10.3389/fnut.2026.1766041

6. Helal MA, El-Gamal AD, Elhela AA, El-Belely EF. Biochemical composition and bioactivity of the crude extract of Sargassum dentifolium (Turner) C. Agardh from the western coast of the Red Sea, Hurghada, Egypt. Biomass Convers Biorefin 2024;14(20):25203–22; doi: https://doi.org/10.1007/s13399-023-04721-9

7. Puspita M, Setyawidati NAR, Stiger-Pouvreau V, Vandanjon L, Widowati I, Radjasa OK, et al. Indonesian Sargassum species bioprospecting: potential applications of bioactive compounds and challenges for sustainable development. In: Seaweeds around the world: state of art and perspectives. Academic Press; 2020. 95, 113 p; doi: https://doi.org/10.1016/bs.abr.2019.12.002

8. Peng Z, Wu Y, Fu Q, Xiao J.Free and bound phenolic profiles and antioxidant ability of eleven marine macroalgae from the South China Sea. Front Nutr 2024;11:1459757; doi: https://doi.org/10.3389/fnut.2024.1459757

9. Mathanmohun M, Sagadevan S, Fatimah I, Lett JA, Kaus NHM, Garg S, et al. Metabolomic characterization of algal nutraceuticals to elucidate their biological activities. Metabolomics 2025;21(6):155; doi: https://doi.org/10.1007/s11306-025-02361-w

10. Sumner LW, Amberg A, Barrett D, Beale MH, Beger R, Daykin CA, et al. Proposed minimum reporting standards for chemical analysis. Metabolomics 2007;3(3):211–21; doi: https://doi.org/10.1007/s11306-007-0082-2

11. Schymanski EL, Jeon J, Gulde R, Fenner K, Ruff M, Singer HP, et al. Identifying small molecules via high-resolution mass spectrometry: communicating confidence. Environ Sci Technol 2014;48(4):2097–8; doi: https://doi.org/10.1021/es5002105

12. Nguyen L, Kim MS, Nguyen NT, Nguyen XT, Cao VL, Nguyen XV, et al. Marine floral biodiversity, threats, and conservation in Vietnam: an updated review. Plants. 2023;12(9):1862. doi: https://doi.org/10.3390/plants12091862

13. Veettil BK, Ward RD, Van DD, Quang NX, Hoai PN. Seagrass ecosystems along the Vietnamese coastline: current state of research and future perspectives. Estuar Coast Shelf Sci 2022;277:108085; doi: https://doi.org/10.1016/j.ecss.2022.108085

14. Schmitt K, Albers T. Mangroves and ecosystem-based coastal protection in the Mekong River Delta, Vietnam. In: Yllano OB ed., Mangrove biology, ecosystem, and conservation. IntechOpen, London, United Kingdom, 2023. doi: https://doi.org/10.5772/intechopen.110820

15. Hieu NT, Ben HX, Dat MX. Species composition and distribution characteristics of seaweeds on seagrass beds in Phu Quoc Island. Vietnam J Mar Sci Technol 2019;19(4A):229–40; doi: https://doi.org/10.15625/1859-3097/19/4a/14602

16. Nguyen VL, Hoang STP. Status and temporal change in the distribution of seagrass beds and coral reefs in the waters of Phu Quoc Islands, Kien Giang Province. Vietnam J Mar Sci Technol 2023;23(1):57–71; doi: https://doi.org/10.15625/1859-3097/16621

17. Sherwood AR, Presting GG. Universal primers amplify a 23S rDNA plastid marker in eukaryotic algae and cyanobacteria. J Phycol 2007;43(3):605–8; doi: https://doi.org/10.1111/j.1529-8817.2007.00341.x

18. AOAC International. Official methods of analysis of AOAC International. 21^st ed. Rockville: AOAC International; 2019.

19. Ismail MM, El Zokm GM, Lopez JMM. Nutritional and bioactive compound contents and antioxidant activity of brown seaweeds from the Red Sea. Front Nutr 2023;10:1210934; doi: https://doi.org/10.3389/fnut.2023.1210934

20. Sari KRP, Ikawati Z, Danarti R, Hertiani T. Micro-titer plate assay for measurement of total phenolic and total flavonoid contents in medicinal plant extracts. Arab J Chem 2023;16:105003; doi: https://doi.org/10.1016/j.arabjc.2023.105003

21. Dewanto V, Wu X, Adom KK, Liu RH. Thermal processing enhances the nutritional value of tomatoes by increasing total antioxidant activity. J Agric Food Chem 2002;50(10):3010–4; doi: https://doi.org/10.1021/jf0115589

22. Lichtenthaler HK, Buschmann C. Chlorophylls and carotenoids: measurement and characterization by UV-VIS spectroscopy. Curr Protoc Food Anal Chem 2001;1:F4.3.1–8; doi: https://doi.org/10.1002/0471142913.faf0403s01

23. Twohig M, O’Leary M, Cleland GE. Methodology for the identification of pesticide metabolites in complex matrices using UPLC-ToF/MS? and the UNIFI Scientific Information System [Internet]. Milford, MA: Waters Corporation; 2015.

24. Ren Y, Wang W, Zhang D, Zhang M, Zhang H, Wu Y, et al. SARS-CoV-2 Mpro inhibitors from Siphonostegia chinensis: an integrated biophysical and computational study. Front Chem 2026;14:1790228; doi: https://doi.org/10.3389/fchem.2026.1790228

25. Leliaert F, Verbruggen H, Vanormelingen P, Steen F, López-Bautista JM, Zuccarello GC, et al. DNA-based species delimitation in algae. Eur J Phycol 2014;49(2):179–96; doi: https://doi.org/10.1080/09670262.2014.904524

26. Álvarez-Canali D, Sansón M, Sangil C, Tronholm A. Multigene phylogenetics of Sargassum (Phaeophyceae) revealed low molecular diversity in contrast to high morphological variability in the NE Atlantic Ocean. J Phycol 2024;60(6):1528–56; doi: https://doi.org/10.1111/jpy.13517

27. Huynh TTH, Vu DP, Nguyen TBN, Nguyen TTX, Do TTT, Dam DD, et al. Combining morphological and molecular data to identify Padina seaweed samples collected from Hon Thom, Phu Quoc, Vietnam. Acad J Biol 2024;46(2):7–18; doi: https://doi.org/10.15625/2615-9023/20583

28. Wirenfeldt CB, Sørensen JS, Kreissig KJ, Hyldig G, Holdt SL, Hansen LT. Post-harvest quality changes and shelf-life determination of washed and blanched sugar kelp (Saccharina latissima). Front Food Sci Technol 2022;2:1030229; doi: https://doi.org/10.3389/frfst.2022.1030229

29. Angell AR, Mata L, De Nys R, Paul NA. The protein content of seaweeds: a universal nitrogen-to-protein conversion factor of five. J Appl Phycol 2016;28(1):511–24; doi: https://doi.org/10.1007/s10811-015-0650-1

30. De Bhowmick G, Hayes M. In vitro protein digestibility of selected seaweeds. Foods 2022;11(3):289; doi: https://doi.org/10.3390/foods11030289

31. Domingues MR, Calado R. Lipids of marine algae—biomolecules with high nutritional value and important bioactive properties. Biomolecules 2022;12(1):134; doi: https://doi.org/10.3390/biom12010134

32. Gientka I, Gadaszewska M, B?a?ejak S, Kieliszek M, Bzducha-Wróbel A, Stasiak-Ró?a?ska L, et al. Evaluation of lipid biosynthesis ability by Rhodotorula and Sporobolomyces strains in medium with glycerol. Eur Food Res Technol 2017;243(2):275–86; doi: https://doi.org/10.1007/s00217-016-2742-9

33. Gientka I, Kieliszek M, Jermacz K, B?a?ejak S. Identification and characterization of oleaginous yeast isolated from kefir and its ability to accumulate intracellular fats in deproteinated potato wastewater with different carbon sources. Biomed Res Int 2017;2017:6061042; doi: https://doi.org/10.1155/2017/6061042

34. Gnayem N, Unis R, Gnaim R, Israel A, Gnaim J, Golberg A. Fatty acid profile in field-collected seaweed, lipid extraction optimization, and food functional properties. Life 2025;15(5):710; doi: https://doi.org/10.3390/life15050710

35. Tang B, Row KH. Development of gas chromatography analysis of fatty acids in marine organisms. J Chromatogr Sci 2013;51(7):599–607; doi: https://doi.org/10.1093/chromsci/bmt005

36. Guihéneuf F, Schmid M, Stengel DB. Lipids and fatty acids in algae: extraction, fractionation into lipid classes, and analysis by gas chromatography coupled with flame ionization detector (GC-FID). In: Stengel DB, Connan S ed.s, Natural products from marine algae: methods and protocols. New York: Humana Press; 2015. 173 p.

37. Lindenmayer R, Lu L, Eivazi F, Afrasiabi Z. Atomic spectroscopy-based analysis of heavy metals in seaweed species. Appl Sci 2023;13(8):4764; doi: https://doi.org/10.3390/app13084764

38. Dujardin B, Ferreira De Sousa R, Gómez Ruiz JA. Dietary exposure to heavy metals and iodine intake via consumption of seaweeds and halophytes in the European population. EFSA J 2023;21(1):7798; doi: https://doi.org/10.2903/j.efsa.2023.7798

39. Jönsson M, Nordberg Karlsson E. Chemical food safety of seaweed: species-, spatial-, and thallus-dependent variation of potentially toxic elements and techniques for their removal. J Appl Phycol 2024;36(2):765–81; doi: https://doi.org/10.1007/s10811-023-03131-8

40. Nguyen LM, Akita S, Cao LV, Nguyen ATM, Pham QV, Vu HM, et al. Concise review of the brown algal genus Padina (Dictyotaceae): knowledge in biodiversity, biogeography, potential, and scope for future research in Vietnam. Vietnam J Mar Sci Technol 2024;24(4):399–418; doi: https://doi.org/10.15625/1859-3097/22126

41. Martínez-López R, Tuohy MG. Rapid and cost-efficient microplate assay for the accurate quantification of total phenolics in seaweeds. Food Chem Mol Sci 2023;6:100166; doi: https://doi.org/10.1016/j.fochms.2023.100166

42. Garcia-Perez P, Lourenço-Lopes C, Silva A, Pereira A, Fraga-Corral M, Zhao C, et al. Pigment composition of nine brown algae from the Iberian northwestern coastline: influence of the extraction solvent. Mar Drugs 2022;20(2):113; doi: https://doi.org/10.3390/md20020113

43. Lourenço-Lopes C, Fraga-Corral M, Garcia-Perez P, Carreira-Casais A, Silva A, Simal-Gandara J, et al. A HPLC-DAD method for identifying and estimating the content of fucoxanthin, β-carotene and chlorophyll a in brown algal extracts. Food Chem Adv 2022;1:100095; doi: https://doi.org/10.1016/j.focha.2022.100095

44. Lopes C, Obando JMC, Santos TCD, Cavalcanti DN, Teixeira VL. Abiotic factors modulating metabolite composition in brown algae (Phaeophyceae): ecological impacts and opportunities for bioprospecting of bioactive compounds. Mar Drugs 2024;22(12):544; doi: https://doi.org/10.3390/md22120544

45. Rapoport A, Guzhova I, Bernetti L, Buzzini P, Kieliszek M, Kot AM. Carotenoids and some other pigments from fungi and yeasts. Metabolites 2021;11(2):92; doi: https://doi.org/10.3390/metabo11020092

46. Kot AM, S?k W, Kieliszek M, B?a?ejak S, Pobiega K, Brzezi?ska R. Diversity of red yeasts in various regions and environments of Poland and biotechnological potential of the isolated strains. Appl Biochem Biotechnol 2024;196(6):3274–316; doi: https://doi.org/10.1007/s12010-023-04705-5

47. Chin YY, Chang KA, Ng WM, Eng ZP, Chew LY, Neo YP, et al. A comparative evaluation of nutritional composition and antioxidant properties of six Malaysian edible seaweeds. Food Chem Adv 2023;3:100426; doi: https://doi.org/10.1016/j.focha.2023.100426

48. Nazarudin MF, Alias NH, Balakrishnan S, Wan Hasnan WNI, Noor Mazli NAI, Ahmad MI, et al. Chemical, nutrient and physicochemical properties of brown seaweed, Sargassum polycystum C. Agardh (Phaeophyceae) collected from Port Dickson, Peninsular Malaysia. Molecules 2021;26(17):5216; doi: https://doi.org/10.3390/molecules26175216

49. Al-Adilah H, Preet G, Astakala RV, Oluwabusola ET, Jaspars M, Ebel R, et al. Chemical profiling of seaweeds of the Arabian Gulf by liquid chromatography-mass spectrometry and in-silico screening against MPOX. Bot Mar 2025;68(3):281–302; doi: https://doi.org/10.1515/bot-2024-0032

50. Melchor-Martínez EM, Reyes AG, Peña-Rodríguez A, Delgado-Cortez IG, Flores-Contreras EA. Utilization of brown algae and pre-treatment strategies as a source of bioactive nutrients for aquaculture species. J Appl Phycol 2025;37(3):2121–46; doi: https://doi.org/10.1007/s10811-025-03508-x

51. Vieira EF, Gomes LR, Grosso C, Delerue-Matos C. Recent advances on seaweed-derived pigments for food application and the current legal framework. Foods 2025;14(18):3265; doi: https://doi.org/10.3390/foods14183265

52. Worley B, Powers R. Multivariate analysis in metabolomics. Curr Metabolomics 2013;1(1):92–107; doi: https://doi.org/10.2174/22132 35X11301010092

53. Saccenti E, Timmerman ME. Approaches to sample size determination for multivariate data: applications to PCA and PLS-DA of omics data. J Proteome Res 2016;15(8):2379–93; doi: https://doi.org/10.1021/acs.jproteome.5b01029

54. Mckinney RE, Cunningham EM, Burton J, Kraan S, Dick JTA, Walsh PJ.Fine-scale biochemical variation in Fucus serratus (Phaeophyceae): point sampling vs bulk samples and implications for industrial applications. J Appl Phycol 2026;38(1):601–12; doi: https://doi.org/10.1007/s10811-025-03756-x

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