Sustainable biodegradation of textile dye reactive blue 222 by the novel strain Enterobacter CU2004, isolated from the industrial waste: A design of experiment based optimization study and characterisation of metabolites

Vasantha Veerappa Lakshmaiah Anish Nag Suresh Gotekar Sunil More Shobha K. Jayanna   

Open Access   

Published:  May 09, 2023

DOI: 10.7324/JABB.2023.70862

Reactive Blue 222 (RB222) is widely used in textile industries and hence a common recalcitrant pollutant in the industrial effluent. Bioremediation of this dye is of significance as its one of the complex dyes with high molecular weight. In the present study, we isolated a novel bacterial strain Enterobacter CU2004 from the industrial waste and characterize using16S rRNA gene sequencing. Its potential to dye degradation was evaluated in a simple minimal salt media with the parameters namely dye concentration (100–1000 ppm), pH (4–9), temperature (15–55°C), Carbon source (Lactose, Sucrose, Glucose, Starch, and Fructose), and Nitrogen source (Casein, Yeast extract, Peptone, Tryptone, Ammonium sulphate, and Urea) in a 24 h culture. Finally, data obtained were extended to design of experiment based optimization for the degradation efficacy of Enterobacter CU2004 and to validated design space was established. The novelty is in optimizing the design space parameters for highest percentage of degradation ≥90% by the bacterial isolate Enterobacter CU2004 were finalized as 30–37°C temperature, 133–249 ppm dye concentration, Lactose as Carbon source, Yeast extract as Nitrogen source, and the pH as 8. Microbial dye degradation was confirmed by FTIR, HPLC and GCMS studies. Further studies revealed the dye intermediates and the potential of Enterobacter CU2004 toward the degradation of complex, high molecular weight industrial dye RB222.

Keyword:     Bioremediation Biodegradation Reactive blue 222 Enterobacter Design of experiment Statistical modeling


Lakshamaiah VV, Nag A, Gotekar S, More S, Jayanna SK. Sustainable biodegradation of textile dye reactive blue 222 by the novel strain Enterobacter CU2004, isolated from the industrial waste: A design of experiment based optimization study and characterisation of metabolites. J App Biol Biotech. 2023. Online First.

Copyright: Author(s). This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.

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