Research Article | Volume: 5, Issue: 1, Jan-Feb, 2017
Plant Growth Promoting Activity of Pink Pigmented Facultative Methylotroph - Methylobacterium extorquens MM2 on Lycopersicon esculentum L.
Pink pigmented facultative methylotrophs (PPFMs) are diversified group of microorganisms that promote plant growth by producing indole acetic acid (IAA) and cytokinins. The objective of the present study is to isolate and characterize methylotrophic bacteria from the phyllosphere of mustard plant (Brassica niagra) and to study their plant growth promotion in tomato (Lycopersicon esculentum L.). Bacteria were isolated from the phyllosphere and the conserved mxaF gene sequence was amplified. Production of IAA was determined by High Performance Thin Layer Chromatography (HPTLC) and further quantified by spectrophotometric analysis. Effect of seed bacterization with the bacterial isolate MM2 was studied for percentage seed germination, root and shoot length. Based on NCBI BLAST search of the mxaF sequence obtained, the bacterial isolate was identified as Methylobacterium extorquens MM2. The amount of IAA produced by M. extorquens MM2 was 6.16 μg/ml. M. extorquens MM2 significantly increased seed vigour index (SVI) in tomato plants. The SVI of the treated plant was 1462.56 ± 83.96 as compared to the control, 1019.94 ± 113.61. Foliar application of M. extorquens MM2 also contributed a substantial impact on growth of tomato plant. The bacterial isolate M. extorquens MM2 thus can be employed in the crops for sustainable agriculture.
Subhaswaraj P, Jobina R, Parasuraman P, Siddhardha B. Plant Growth Promoting Activity of Pink Pigmented Facultative Methylotroph-Methylobacterium extorquens MM2 on Lycopersicon esculentum L. J App Biol Biotech. 2017; 5 (01): 042-046. DOI: 10.7324/JABB.2017.50107
1. Ochsner AM, Sonntag F, Buchhaupt M, Schrader J, Vorholt JA. Methylobacterium extorquens: methylotrophy and biotechnological applications. Applied Microbiology and Biotechnology. 2015; 99: 517-534.
2. Raja D, Sivasankari B, Thilagavathy D. Bioefficacy of Methylobacteriurn spp. isolated from various leaf samples on the growth performance of black gram, Vigna mungo L. walp. Journal of Current Science. 2008; 12: 735-740.
3. Madhaiyan M, Poonguzhali S, Senthilkumar M, Lee JS, Lee KC. Methylobacterium gossipiicola sp. nov., a pink pigmented, facultatively methylotrophic bacterium isolated from the cotton phyllosphere. International Journal of Systematic and Evolutionary Microbiology. 2012; 62: 162-167.
4. Jayashree S, Vadivukkarasi P, Anand K, Kato Y, Seshadri S. Evaluation of pink-pigmented facultative methylotrophic bacteria for phosphate solubilization. Archives of Microbiology. 2011; 193: 543-552.
5. Meena KK, Kumar M, Kalyuzhnaya MG, et al. Epiphytic pink-pigmented methylotrophic bacteria enhance germination and seedling growth of wheat (Triticum aestivum) by producing phytohormone. Antonie van Leeuwenhoek. 2012; 101: 777-786.
6. Mizuno M, Yurimoto H, Iguchi H, Tani A, Sakai Y. Dominant colonization and inheritance of Methylobacterium species strain OR01 on Perilla plants. Bioscience Biotechnology and Biochemistry. 2013; 77: 1533-1538.
7. Tani A, Sahin N, Kimbara K. Methylobacterium gnaphalii sp. nov., isolated from leaves of Gnaphalium spicatum. International Journal of Systematic and Evolutionary Microbiology. 2012; 62, 2602-2607.
8. Kwak MJ, Jeong H, Madhaiyan M. Genome Information of Methylobacterium oryzae, a Plant-Probiotic Methylotroph in the Phyllosphere. PLoS ONE. 2014; 9: 1-14.
9. SriBalaji R, Dhamodharan G, Rajasekar A. Isolation and Characterization Of Methylotrophic Bacteria From Western Ghats. International Journal of Engineering Research and Technology. 2013; 2:1752-175.
10. Jayashree S, Lalitha R, Vadivukkarasi P, Kato Y, Seshadri S. Cellulase Production by Pink Pigmented Facultative Methylotrophic Strains (PPFMs). Applied Biochemistry and Biotechnology. 2011; 164: 666-680.
11. Laxmi J, Palanichamy V, Narayana Reddy N, Rajsekaran C, Nancy VeenaKumari D, Bhaskarmitra. Standardization of Cultivation Parameters for The Extraction of Carotenoid From Pink Pigmented Facultative Methylotrophic (PPFM) Bacteria. Asian Journal of Pharmaceutical and Clinical Research. 2011; 5: 52-57.
12. Knief C, Dengler V, Bodelier PLE, Vorholt JA. Characterization of Methylobacterim strains isolated from the phyllosphere and description of Methylobacterium longum sp. nov. Antonie Van Leeuwenhoek. 2012; 101: 169-183.
13. Dourado MN, Andreote FD, Dini-Andreote1 F, Conti R, Araujo JM, Araujo WL. Analysis of 16S rRNA and mxaF genes revealing insights into Methylobacterium niche-specific plant association. Genetics and Molecular Biology. 2012; 35: 142-148.
14. Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S. MEGA5: Molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Molecular Biology and Evolution. 2011; 28(10):2731-2739.
15. Goswami D, Thakker JN, Dhandhukia PC. Simultaneous detection and quantification of indole-3-acetic acid (IAA) and indole-3-butyric acid (IBA) produced by rhizobacteria from L-tryptophan (Trp) using HPTLC. Journal of Microbiological Methods. 2015; 110: 07-14.
16. Rahman A, Sitepu IR, Tang SY, Hashidoko Y. Salkowski’s reagent Test as a Primary Screening Index for Functionalities of Rhizobacteria Isolated from Wild Dipterocarp Saplings Growing Naturally on Medium-Strongly Acidic Tropical Peat Soil. Bioscience Biotechnology and Biochemistry. 2010; 74: 2202-8.
17. Boruah HPD, Chauhan PS, Yim WJ, Han GH, Sa TM. Comparison of Plant Growth Promoting Methylobacterium spp. And Exogenous Indole-3-Acetic Acid Application on Red Pepper and Tomato Seedling Development. Korean Journal of Soil Science and Fertilizer. 2010; 43(1): 96-104.
18. Madhaiyan M, Suresh Reddy BV, Anandham R. Plant Growth-Promoting Methylobacterium Induces Defense Response in Groundnut (Arachis hypogaea L.) Compared with Rot Pathogens. Current Microbiology. 2006; 53: 270-276.
19. Dourado MN, Neves AAC, Santos DZ, Araujo WL. Biotechnological and Agronomic Potential of Endophytic Pink-Pigmented Methylotrophic Methylobacterium spp. BioMed Research International. 2015.
20. Omer ZS, Tombolini R, Broberg A, Gerhardson B. Indole-3-acetic acid production by pink-pigmented facultative methylotrophic bacteria. Plant Growth Regulation. 2004; 43: 93-96.
Year
Month
Evaluation of Bacillus subtilis MRB4, as plant growth promoter and potential phosphate solubilizer under abiotic stress
Nishat Khatoon, Mazharuddin KhanMicrobial diversity of Azadirachta indica (Neem) gum: An unexplored niche
Pragya Saxena,, Hillol Chakdar, Arjun Singh, Sheetal Shirodkar, Alok K. SrivastavaExperimental studies on isolation and characterization of silicate solubilizing Bacillus tequilensis SKSSB09
Karuganti Sukumar, Ganti Swarnabala, Muralitharan Gangatharan