Research Article | Volume: 5, Issue: 1, Jan-Feb, 2017

Klebsiella pneumoniae VRE36 as a PGPR isolated from Saccharum officinarum cultivar Co99004

Gurvesh Bhardwaj Rushabh Shah Bhrugesh Joshi Prittesh Patel   

Open Access   

Published:  Jan 20, 2017

DOI: 10.7324/JABB.2017.50108

Klebsiella species known to exhibit important PGP traits like solubilization of phosphate, phytohormone production and good germination potential. In present study, based on the Phosphate solubilization and IAA production bacterial strain VRE36 was selected among the isolates collected from different sugarcane cultivar rhizosphere growing near Bardoli area for characterization and molecular identification through 16S rRNA gene sequence, which confirms the isolate as Klebsiella pneumoniae. The phosphate solubilization index of the isolate recorded was 3.9 and quantitative estimation reveals 17.4±1.78µg/ml release of phosphate in NBRIP broth. The high amount of IAA produced was 45.32±2.46µg/ml after 96 hour incubation at 37 °C. In seed germination assay with V radiate, C tetragonoloba and V unguiculata, treatments with bacteria were supported good plant height, dry weight and fresh weight when compared with controls. Maximum percentage of germination was recorded in V radiate (97.78%). The improved seedling parameters of the inoculated crop seeds indicated the potential of this isolate to be used in a bio-fertilizer formulation for sustainable production.

Keyword:     Klebsiella pneumoniae PGPR Phosphate solubization Seed germination Sugarcane.


Bhardwaj G, Shah R, Joshi B, Patel P. Klebsiella pneumoniae VRE36 as a PGPR isolated from Saccharum officinarum cultivar Co99004. J App Biol Biotech. 2017; 5 (01): 047-052. DOI: 10.7324/JABB.2017.50108

Copyright: Author(s). This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.

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1. Bhattacharyya P, Jha D. Plant growth-promoting rhizobacteria (PGPR): emergence in agriculture. World Journal of Microbiology and Biotechnology. 2012; 28(4):1327-1350.

2. Qureshi MA, Ahmad ZA, Akhtar N, Iqbal A. Role of phosphate solubilizing bacteria (PSB) in enhancing P-availibility and promoting cotton growth. Journal of Animal-Plant Science. 2012; 22:204-210.

3. Vessey JK. Plant growth promoting rhizobacteria as biofertilizers. Plant and soil. 2003; 255(2):571-586.

4. Glick BR, Karaturovíc DM, Newell PC. A novel procedure for rapid isolation of plant growth promoting pseudomonads. Canadian Journal of Microbiology. 1995; 41(6):533-536.

5. Das AC, Mukherjee D. Influence of insecticides on microbial transformation of nitrogen and phosphorus in Typic Orchragualf soil. Journal of Agricultural and Food Chemistry. 2000; 48(8):3728-3732.

6. Kundu B, Gera R, Sharma N, Bhatia A, Sharma R. Host specificity of phosphate solubilizing bacteria. Indian Journal of Microbiology. 2002; 42(1):19-21.

7. Aris T. Wahyudi RIA. Screening of Pseudomonas sp. isolated from rhizosphere of soybean plant as plant growth promoter and biocontrol agent. American Journal of Agricultural and Biological Sciences. 2011; 6(1):134-141. doi: 10.3844/ajabssp.2011.134.141

8. Hafeez FY, Yasmin S, Ariani D, Zafar Y, Malik K. A. Plant growth-promoting bacteria as biofertilizer. Agronomy for Sustainable Development. 2006; 26(2):143-150.

9. Jorquera MA, Hernández MT, Rengel Z, Marschner P, Mora M. Isolation of culturable phosphobacteria with both phytate-mineralization and phosphate-solubilization activity from the rhizosphere of plants grown in a volcanic soil. Biology and Fertility of Soils. 2008; 44(8):1025-1034.

10. Rodrıguez H, Gonzalez T, Selman G. Expression of a mineral phosphate solubilizing gene from Erwinia herbicola in two rhizobacterial strains. Journal of Biotechnology. 2000; 84(2):155-161.

11. Cappuccino JGS, Cappuccino NJG, Sherman N. Microbiology: a laboratory manual. 1996.

12. Pikovaskaya RI. Mobilization of phosphorous in soil in connection with the vital activity of some microbial species. Microbiologia. 1948; 17:362-370.

13. Premono ME, Moawad A, Vlek P. Effect of phosphate-solubilizing Pseudomonas putida on the growth of maize and its survival in the rhizosphere. Indonesian Journal of Crop Science. 1996; 11:13-23.

14. Bric JM., Bostock RM, Silverstone SE. Rapid in situ assay for indoleacetic acid production by bacteria immobilized on a nitrocellulose membrane. Applied and Environmental Microbiology. 1991; 57(2):535-538.

15. Sambrook J, Russell DW. Molecular cloning: a laboratory manual 3rd edition. Coldspring-Harbour Laboratory Press, UK. 2001.

16. Kumar A, Maurya BR, Raghuwanshi R. Isolation and characterization of PGPR and their effect on growth, yield and nutrient content in wheat (Treaticum aestivum L.). Biocatalyst Agriculture and Biotechnology. 2014; 3:121-128.

17. Ruangsanka S. Identification of phosphate-solubilizing fungi from the asparagus rhizosphere as antagonists of the root and crown rot pathogen Fusarium oxysporum. ScienceAsia. 2014; 40:16-20.

18. Islam M, Deora A, Hashidoko Y, Rahman A, Ito T, Tahara S. Isolation and identification of potential phosphate solubilizing bacteria from the rhizoplane of Oryza sativa L. cv. BR29 of Bangladesh. Zeitschrift für Naturforschung C. 2007; 62(1-2):103-110.

19. Ogbo F, Okonkwo J. Some characteristics of a plant growth promoting Enterobacter sp. isolated from the roots of maize. Advances in Microbiology. 2012; 2(3):368-374.

20. Widawati S, Suliasih HL, Sugiharto A. Biodiversity of Soil Microbes from Rhizosphere at Wamena Biological Garden (WBiG), Jayawijaya, Papua. Biodiversitas. 2005; 6(1):6-11.

21. Henry K, Baharuddin, Bandron Z, Syatrianty AS. Isolation and physiological characterization of PGPR from potato plant rhizosphere in medium land of Buru Island. Procedia Food Science. 2015; 3:190-199.

22. Zahid M, Abbasi MK, Hameed S, Rahim N. Isolation and identification of indegenous plant growth prmoting rhizobacteria from himalayan region of kashmir and their effect on improving growth and nutrient contents of maize (Zea mais L.). Frontiers in Microbiology. 2015; 6(207). Doi: 10.3389/fmicb.2015.002017

23. Duca D, Lorv J, Patten CL, Rose D, Glick BR. Indole-3-acetic acid in plant microbe interactions. Antonie Van Leeuwenhoek.

24. Benizir E, Caurtade A, Picard C, Guckert A. Role of maize root exudates in the production of auxins by Pseudomonas flauroscens. Soil Biology and Biochemistry. 1998; 30:1481-1484.

25. Govindarajan M, Kwon SW, Weon HY. Isolation, molecular characterization and growth-promoting activities of endophytic sugarcane diazotroph Klebsiella sp. GR9. World Journal of Microbiology and Biotechnology. 2007; 23(7):997-1006.

26. El-Khawas H, Adachi K. Identification and quantification of auxins in culture media of Azospirillum and Klebsiella and their effect on rice roots. Biology and Fertility of Soils. 1999; 28(4):377-381.

27. Sobral J, Araújo WL, Mendes R, Geraldi IO, Kleiner AA, Azevedo JL. Isolation and characterization of soybean‐associated bacteria and their potential for plant growth promotion. Environmental Microbiology. 2004; 6(12):1244-1251.

28. Sachdev DP, Chaudhari HG, Kasture VM, Dhavale DD, Chopade BA. Isolation and characterization of indole acetic acid (IAA) producing Klebsiella pneumoniae strains from rhizosphere of wheat (Triticum aestivum) and their effect on plant growth. Indian Journal of Experimental Biology. 2009; 47(12):993.

29. Hayat R, Ali S, Amara U, Khalid R, Ahmed I. Soil benificial bacteria and their role in plant growth promotion: a review. Annals of Microbiology. 2010; 60:579-598.

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