Research Article | Volume: 2, Issue: 2, March-April, 2014
Gene expression study of Saccharomyces cerivisae GPH1 gene in response to chemical modulators
The aim of the present study was to evaluate the gene expression of GPH1 gene of Saccharomyces cerivisae after it was induced to different stress conditions. The S. cerivisae was procured from MTCC and grown in YPD medium with different modulators like NaCl, Glucose, Calcium chloride, Sodium chloride, Potassium Nitrate, Arabinose and Dipotassium hydrogen orthophosphate. The RNA was isolated and analyzed on formaldehyde gel electrophoresis and determined by Nanodrop spectrophotometer. The cDNA was synthesized and used for Real Time PCR. Quantification was based on a standard curve, prepared from samples of known template concentration. The concentration of unknown sample is then determined by Ct value. There is a varied response to glycogen metabolism in S. cerivisae to environmental stress conditions such as nutrient limitation and heat shock. This response is mediated, in part, by the regulation of the glycogen metabolic genes. The results indicated that gene expression was high in modulator 3 and lowest in 2. Environmental stress induces a number of glycogen metabolic genes, including GPH1, which encodes glycogen phosphorylase. This study confirmed induction patterns of gene encoding glycogen phosphorylase GPH1 after treatment with modulators. This study gives an insight to the transcriptional control of GPH1 after modulator treatment.
Prasad M.P. Gene expression study of Saccharomyces cerivisae GPH1 gene in response to chemical modulators. J App Biol Biotech, 2014; 2 (02): 016-018.
1. Barber A. R., Vriesekoop F., Pamment N. B. Effect of acetaldehyde on Saccharomyces cerevisiae exposed to range of chemical and environmental stresses. Enzyme and Microbial Technology. 2002; 30: 240-250.
2. Stanley G. A. Douglas N. G., Everu E. J. Tzanatos T. and Pamment N. B.. Inhibition and stimulation of yeast growth by acetaldehydee. Biotechnology letters. 1993; 15:199-1204.
3. Stanley G. A., Hobley T. J. & Pamment N. B. Effect of acetaldehyde on Saccharomyces cerevisiae and Zymomona mobilis subjected to environmental shocks. Biotechnology and Bioengineering. 1997; 53:71-78.
4. Vriesekoop F. and Pamment N. B. Acetaldehyde addition and pre-adaptation to the stressor together virtually eliminate the ethanol-induced lag phase in Saccharomyces cerevisiae. 2005; 41(5):424-427.
5. Walker-Caprioglio H. M. and Parks L. W. Autconditioning factor relieves ethanol-induced growth inhibition of Saccharomyces cerevisiae. Applied Environmental Microbiology.1987; 50:685-689.
6. Birch R. M. & Walker G. M. Influence of magnesium ions on heat shock and ethnaol stress responses of Saccharomyces cerevisiae. Enzyme and Microbial Technology. 2000; 26:678-687.
7. Gasch A. P., Huang M. X., Metzner S., Botstein D., Elledge S. J. and Brown P. O. Genomic expression responses to DNA damaging agents and the regulatory role of yeast ATR homolog Mec1p. Molecular Biology of the Cell. 2001; 12:2987-3003.
8. Mager W. H. & Hohmann S. Stress response mechanisms in the yeast Saccharomyces cerevisiae. In Yeast Stress Responses. 1997; 1-5.
9. Hu C. K., Bai F. W. and An L. J. Enhance ethanol tolerance of a selfflocculating fusant of Schizosaccharomyces Pombe and Saccharomyces cerevisiae Mg2+ via reduction in plasma membrane permeability. Biotechnology letters. 2003; 25(14):1191-1194.
10. Walker, G. M. The role of magnesium in biotechnology. Critical Reviews in Biotechnology. 1994; 14:311-354.
11. Chatterjee M. T., Khalawan S. A. and Curran B. P. G. Cellular lipid composition influences stress activation of the yeast general stress response element (STRE). Microbiology. 2000; 146:877-884.
12. Ruis H. and Shuller C. Stress signalling in yeast. Bioassays. 1995; 17(11):959-965.
13. Mager W. H. & Moradas-Ferreira P. Stress response of yeast. Journal of Biochemistry. 1993; 290:1-13.
14. Piper P. W. Molecular events associated with acquisition of heat tolerance by yeast Saccharomyces cerevisiae. FEMS Microbiology Reviews. 1993; 11:339-356.
15. Siderius M. & Mager W. H. General stress response: in search of a common denominator. In Yeast Stress Responses. 1997; 213-229.
16. Hottiger T., Boller T. and Wiemkem A. Rapid changes of heat and desiccation tolerance correlated with changes of trehalose content in Saccharomyces cerevisiae cell subject to temperature shifts. FEBS Letters. 1987; 220:113-115.
17. Lewis J. G., Learmonth P. R. and Watson K. Induction of heat, freezing and salt tolerance by heat and salt shock in Saccharomyces cerevisiae. Microbiology 1995; 141:687-694.
18. VanGuilder HD, Vrana KE, Freeman WM: Twenty-five years of quantitative PCR for gene expression analysis. Biotechniques. 2008; 44(5):619-626.
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