Research Article | Volume: 6, Issue: 3, May-June, 2018

Coleoptilar node - A season-independent explant source for in vitro culture in maize (Zea mays L.)

Pavan Kumar Gudlavalleti Sreenu Pagidoju Sridevi Muppala Reddy Malireddy Kodandarami Sateesh Kumar Puligandla   

Open Access   

Published:  Apr 05, 2018

DOI: 10.7324/JABB.2018.60304
Abstract

In vitro plant regeneration is preliminary requisite in the process of obtaining transgenic plants. Somatic embryogenesis is the most frequently used method of in vitro plant regeneration in maize. In this study, a reliable and reproducible maize regeneration method has been standardized using mature embryos. Inspite, the immature embryos are most suitable explants for somatic embryogenesis, obtaining these explants continuously is a laborious and tedious process, especially in summer. Hence, alternate explants of maize for in vitro regeneration have been chosen from different parts of embryonic axis. This study provides an evaluation of inbred lines and effect of hormonal combinations on total and embryogenic callus induction, their growth rate, and plantlet regeneration. Coleoptilar nodal explants from four inbred lines were cultured on different concentrations and combinations of 2,4-dichloro phenoxyacetic acid (2,4-D) along with kinetin (Kn). Explants grown on Murashige and Skoog medium supplemented with 2,4-D and Kn at a concentration of 5 and 1 mg/L, respectively, were found to be the best one for yielding a high frequency of embryogenic callus. Inbred line, NM81A was found to be the best one for in vitro culture among the four lines used. Combination of 6-benzylaminopurine and Kn at a concentration of 1 mg/L each promoted the highest frequency of shoot induction.


Keyword:     Coleoptilar node In vitro regeneration Growth rate Zea mays L.


Citation:

Gudlavalleti PK, Pagidoju S, Muppala S, Kodandarami RM, Puligandla SK. Coleoptilar node - A season-independent explant source for in vitro culture in maize (Zea mays L.). J App Biol Biotech. 2018;6(3):20-28. DOI: 10.7324/JABB.2018.60304

Copyright: Author(s). This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.

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