Research Article | Volume 13, Supplement 1, July, 2025
Assessment of nanofertilizers’ effect on yield of seedling biomass in vegetable crops
Modern agriculture relies heavily on chemical fertilizers, which can lead to environmental issues. To address these challenges, nanofertilizers offer a potential solution by improving nutrient delivery and reducing environmental impact. This study explores the synthesis and effects of nanoparticles (NPs) on seed germination and seedling growth. The NPs (ZnO, Fe2O3, and MnO2) were greenly synthesized by using pea peel biomass and were characterized by UV-visible spectroscopy, Fourier transform infrared spectroscopy (FTIR), and field emission scanning electron microscopy (FESEM), confirming their size and shape. The sterilized seeds of tomato, chili, and brinjal were treated with aqueous solutions of NPs at concentrations of 10, 20, and 50 ppm. The seeds were sprayed at 48-hour intervals. The germination rates and growth parameters such as shoot and root length, as well as fresh and dry weight measured. UV-visible spectra revealed absorption peaks at 357 nm for ZnO, 333 nm for Fe2O3, and 360 nm for MnO2. FTIR and FESEM confirmed the successful synthesis and characterized the NPs’ sizes and shapes. Notably, MnO2 and their combinations of NPs enhanced seedling growth and biomass as compared to control. The findings highlight that green-synthesized MnO2 NPs effectively promote seed germination and seedling growth. These eco-friendly nanofertilizers offer a promising approach to enhancing crop yield and supporting sustainable agriculture.
Kanwal A, Behl RK, Singh I, Tiwari NK, Kumar S, Sehgal R, Singh BJ, Singh R. Assessment of nanofertilizers’ effect on yield of seedling biomass in vegetable crops. J Appl Biol Biotech. 2025;13 (Suppl 1):53–61. https://doi.org/10.7324/JABB.2025.211140
1. Mohammed SS, Lawrance AV, Sampath S, Sunderam V, Madhavan Y. Facile green synthesis of silver NPss from sprouted Zingiberaceae species: spectral characterisation and its potential biological applications. Mater Tech 2022;37:533–46. https://doi.org/10.1080/10667857.2020.1863571
2. Bala N, Saha S, Chakraborty M, Maiti M, Das S, Basu R, et al. Green synthesis of zinc oxide NPss using Hibiscus subdariffa leaf extract: effect of temperature on synthesis, anti-bacterial activity and anti-diabetic activity. RSC Adv 2015;5:4993–5003. https://doi.org/10.1039/C4RA12784F
3. Khaleghi S, Khayatzadeh J, Neamati A. Biosynthesis of Zinc Oxide NPss using Origanum majorana L. leaf extract, its antioxidant and cytotoxic activities. Adv Mater Technol 2022;37:2522–31. https://doi.org/10.1080/10667857.2022.2044218
4. Mittal AK, Chisti Y, Banerjee UC. Synthesis of metallic NPss using plant extracts. Biotechnol Adv 2013;31:346–56. https://doi.org/10.1016/j.biotechadv.2013.01.003
5. Jadoun S, Chauhan NP, Zarrintaj P, Barani M, Varma RS, Chinnam S, et al. Synthesis of NPss using microorganisms and their applications: a review. Environ Chem Lett 2022;20:3153–97. https://doi.org/10.1007/s10311-022-01444-7
6. Lin J, He F, Su B, Sun M, Owens G, Chen Z. The stabilizing mechanism of cadmium in contaminated soil using green synthesized iron oxide NPss under long-term incubation. J Hazard Mater 2019;379:120832. https://doi.org/10.1016/j.jhazmat.2019.120832
7. Balafrej H, Bogusz D, Triqui ZEA, Guedira A, Bendaou N, Smouni A, et al. Zinc hyperaccumulation in plants: a review. Plants 2020;9:562; https://doi.org/10.3390/plants9050562
8. Ahmed R, Yusoff Abd Samad M, Uddin MK, Quddus MA, Hossain MM. Recent trends in the foliar spraying of zinc nutrient and zinc oxide NPss in tomato production. Agron 2021;11:2074; https://doi.org/10.3390/agronomy11102074
9. Dimkpa CO, Singh U, Adisa IO, Bindraban PS, Elmer WH, Gardea-Torresdey JL, et al. Effects of manganese NPs exposure on nutrient acquisition in wheat (Triticum aestivum L.). Agron 2018;8:158.
10. Rui M, Ma C, Hao Y, Guo J, Rui Y, Tang X, et al. Iron oxide NPss as a potential iron fertilizer for peanut (Arachis hypogaea). Front. Plant Sci 2016;7:815; https://doi.org/10.3389/fpls.2016.00815
11. Lucena JJ, Gárate A, Villén M. Stability in solution and reactivity with soils and soil components of iron and zinc complexes. J Plant Nutr Soil Sci 2010;173:900–6; https://doi.org/10.1002/jpln.200900154
12. Cieschi MT, Polyakov AY, Lebedev VA, Volkov DS, Pankratov DA, Veligzhanin AA, et al. Eco-friendly iron-humic nanofertilizers synthesis for the prevention of iron chlorosis in soybean (Glycine max) grown in calcareous soil. Front Plant Sci 2019;10:448294; https://doi.org/10.3389/fpls.2019.00413
13. Gevers LE, Enakonda LR, Shahid A, Ould-Chikh S, Silva CI, Paalanen PP, et al. Unraveling the structure and role of Mn and Ce for NOx reduction in application-relevant catalysts. Nat Commun 2022;13:2960; https://doi.org/10.1038/s41467-022-30679-9
14. Ogunyemi SO, Zhang F, Abdallah Y, Zhang M, Wang Y, Sun G, et al. Biosynthesis and characterization of magnesium oxide and manganese dioxide NPss using Matricaria chamomilla L. extract and its inhibitory effect on Acidovorax oryzae strain RS-2. Artif. Cells Nanomed Biotechnol 2019;47:2230–9; https://doi.org/10.1080/21691401.2019.1622552
15. Hassanisaadi M, Barani M, Rahdar A, Heidary M, Thysiadou A, Kyzas GZ. Role of agrochemical-based nanomaterials in plants: biotic and abiotic stress with germination improvement of seeds. Plant Growth Regul 2022;97:375–418.
16. Renna M, Durante M, Gonnella M, Buttaro D, D’Imperio M, Mita G, et al. Quality and nutritional evaluation of regina tomato, a traditional long-storage landrace of Puglia (Southern Italy). Agric 2018;8:83; https://doi.org/10.3390/agriculture8060083
17. Hemannavar V. Studies on seed borne aspects of anthracnose of chilli and its management. M. Sc. (Agri.) Thesis 2008. Available via https://www.researchgate.net/publication/273676660
18. Anonymous FAO. Statistical Year Book. Food and Agriculture Organization UN, Rome, Italy, pp 75–6, 2014.
19. Weese TL, Bohs L. Eggplant origins: out of Africa, into the Orient. Taxon 2010;59:49–56; https://doi.org/10.1002/tax.591006
20. Daunay MC, Lester RN, Hennart JW, Duranton C. Eggplants: present and future. Capsicum Eggplant Newslett 2000;19:11–8; https://www.researchgate.net/publication/341855441
21. Narayanamma A, Rani A, Raju ME. Natural synthesis of silver NPss by banana peel extract and as an antibacterial agent. IOSR J Polymer Text Eng 2016;3:17–25.
22. Üstün E, Önba? SC, Çelik SK, Ayvaz MÇ, ?ahin N. Green synthesis of iron oxide NPss by using Ficus carica leaf extract and its antioxidant activity. Biointerface Res Appl Chem 2022;12(2):2108–16. https://doi.org/10.33263/BRIAC122.21082116
23. Siddiqui MH, Al-Whaibi MH. Role of nano-SiO2 in germination of tomato (Lycopersicum esculentum seeds Mill.). Saudi J Biol Sci 2014;21:13–7. https://doi.org/10.1016/j.sjbs.2013.04.005
24. Arora V, Singh BJ, Bithel N, Malik N, Upadhyay SK, Singh R. Effect of simulated acid rain on plant growth behaviour of Solanum melongena Linn. and Vigna unguiculata ssp cylindrica (L.) Walp. Environ Dev Sustain 2024;26:627–55. https://doi.org/10.1007/s10668-022-02726-4
25. Thi Tran QM, Thi Nguyen HA, Doan VD, Tran QH, Nguyen VC. Biosynthesis of zinc oxide NPss using aqueous piper betle leaf extract and its application in surgical sutures. J Nanomater 2021;2021:8833864. https://doi.org/10.1155/2021/8833864
26. Hoseinpour V, Souri M, Ghaemi N. Green synthesis, characterisation, and photocatalytic activity of manganese dioxide NPs. Nanomicro Lett 2018;13:1560–3. https://doi.org/10.1049/mnl.2018.5008
27. Jamdagni P, Khatri P, Rana JS. Green synthesis of zinc oxide NPss using flower extract of Nyctanthes arbor-tristis and their antifungal activity. J King Saud Univ Sci 2018;30:168–75. https://doi.org/10.1016/j.jksus.2016.10.002
28. El-Belely EF, Farag MM, Said HA, Amin AS, Azab E, Gobouri AA, et al. Green synthesis of zinc oxide NPs (ZnO-NPs) using Arthrospira platensis (Class: Cyanophyceae) and evaluation of their biomedical activities. Nanomater 2021;11:95. https://doi.org/10.3390/nano11010095
29. Morales-Díaz AB, Ortega-Ortíz H, Juárez-Maldonado A, Cadenas-Pliego G, González-Morales S, Benavides-Mendoza A. Application of nanoelements in plant nutrition and its impact in ecosystems. Adv Nat Sci Nanosci Nanotechnol 2017;8:013001; https://doi.org/10.1088/2043-6254/8/1/013001
30. Khodakovskaya MV, De Silva K, Biris AS, Dervishi E, Villagarcia H. Carbon nanotubes induce growth enhancement of tobacco cells. ACS Nano 2012;6:2128–35; https://doi.org/10.1021/nn204643g
31. Chen H, Yada R. Nanotechnologies in agriculture: new tools for sustainable development. Trends Food Sci Technol 2011;22:585–94; https://doi.org/10.1016/j.tifs.2011.09.004
32. Kanwal A, Sharma I, Bala A, Upadhyay SK, Singh R. Agricultural application of synthesized ZnS NPss for the development of tomato crop. Lett Appl NanoBioSci 2022;12:1–9; https://doi.org/10.33263/LIANBS122.058
33. Kumar M, Saini RV, Gupta M, Singh, R. Green synthesis of silver NPs (Cha-AgNPs) using Chenopodium album extract and evaluation of their antifungal potential against pathogenic fungi. Biomass Convers Biorefin 2024;1–12; https://doi.org/10.1007/s13399-024-05721-z
34. Madzokere TC, Murombo LT, Chiririwa HJ. Nano-based slow releasing fertilizers for enhanced agricultural productivity. Mater Today Proc 2021;45:3709–15.
35. Abdel-Aziz H. Effect of priming with chitosan NPss on germination, seedling growth and antioxidant enzymes of broad beans. Catrina Int J Environ Sci 2019;18:81–6.
36. Raja K, Sowmya R, Sudhagar R, Moorthy PS, Govindaraju K, Subramanian KS. Biogenic ZnO and Cu NPss to improve seed germination quality in blackgram (Vigna mungo). Mater Lett 2019;235:164–7; https://doi.org/10.1016/j.matlet.2018.10.038
37. Liu X, Wang, F, Shi Z, Tong R, Shi X. Bioavailability of Zn in ZnO NPs-spiked soil and the implications to maize plants. J NPs Res 2015;17:1–11; https://doi.org/10.1007/s11051-015-2989-2
38. Panwar J. Positive effect of zinc oxide NPss on tomato plants: a step towards developing nano-fertilizers. In International conference on environmental research and technology (ICERT). 2012, University of Sains, Malaysia; doi: https://doi.org/10.13140/2.1.2697.8889
39. Tarafdar JC, Raliya R, Mahawar H, Rathore I. Development of zinc nanofertilizer to enhance crop production in pearl millet (Pennisetum americanum). Agri Res 2014;3:257–62; https://doi.org/10.1007/s40003-014-0113-y
40. El Rasafi T, Nouri M, Bouda S, Haddioui A. The effect of Cd, Zn and Fe on seed germination and early seedling growth of wheat and bean Ekológia (Bratislava). 2016;35:213–23; https://doi.org/10.1515/eko-2016-0017
41. García-López JI, Zavala-García F, Olivares-Sáenz E, Lira-Saldívar RH, Díaz Barriga-Castro E, Ruiz-Torres NA, et al. Zinc oxide NPss boosts phenolic compounds and antioxidant activity of Capsicum annuum L. during germination. Agron 2018;8:215; https://doi.org/10.3390/agronomy8100215
42. Pandey AC, Sanjay S, Yadav R. Application of ZnO NPss in influencing the growth rate of Cicer arietinum. J Exp Nanosci 2010;5:488–97; https://doi.org/10.1080/17458081003649648
43. Prasad TNVKV, Sudhakar P, Sreenivasulu, Y, Latha P, Munaswamy V, Reddy KR, et al. Effect of nanoscale zinc oxide particles on the germination, growth and yield of peanut. J Plant Nutri 2012;35:905–27; https://doi.org/10.1080/01904167.2012.663443
44. Valadkhan M, Mohammadi K, Nezhad MK. Effect of priming and foliar application of NPss on agronomic traits of chickpea. Biol Forum. 2015;7:599–602; http://researchtrend.net/pdf/97%20KHOSRO%20MOHAMMADI.pdf
45. Abusalem M, Awwad A, Ayad J, Rayyan AA. Green synthesis of α-Fe2O3 NPs using Pistachio leaf extract influenced seed germination and seedling growth of tomatos. Jordan J Earth Environ Sci 2019;10 https://www.researchgate.net/publication/336444838
46. Karunakaran G, Jagathambal M, Van Minh N, Kolesnikov E, Gusev A, Zakharova OV, et al. Green synthesized iron oxide NPss: a nano-nutrient for the growth and enhancement of flax (Linum usitatissimum L.) plant. Int. J Biotechnol Bioeng 2017;11:289–93; https://doi.org/10.5281/zenodo.1129910
47. Hu J, Wu C, Ren H, Wang Y, Li J, Huang J. Comparative analysis of physiological impact of γ-Fe2O3 NPss on dicotyledon and monocotyledon. J Nanosci Nanotechnol 2018;18:743–52; https://doi.org/10.1166/jnn.2018.13921
48. Pradhan S, Patra P, Das S, Chandra S, Mitra S, Dey KK, et al. Photochemical modulation of biosafe manganese NPss on Vigna radiata: a detailed molecular, biochemical, and biophysical study. Environ Sci Technol 2013;47:13122–31; doi: https://doi.org/10.1021/es402659t
49. Elmer WH, White JC. The use of metallic oxide NPs to enhance growth of tomatoes and eggplants in disease infested soil or soilless medium. Environ Sci Nano 2016;3:1072–9; doi: https://doi.org/10.1039/C6EN00146G
50. Abobatta WF. Nanotechnology application in agriculture. Acta Sci Agric 2018;2:99–102.
51. Salama DM, Abd El-Aziz ME, Rizk FA, Abd Elwahed MSA. Applications of nanotechnology on vegetable crops. Chemosphere 2021;266:129026; doi: https://doi.org/10.1016/j.chemosphere.2020.129026
52. Shebl A, Hassan AA, Salama DM, Abd El-Aziz ME, Abd Elwahed MSA. Template-free microwave assisted hydrothermal synthesis of manganese zinc ferrite as a nanofertilizer for squash plant (Cucurbita pepo L). Heliyon 2020;6:e03596; doi: https://doi.org/10.1016/j.heliyon.2020.e03596
53. Elumalai K, Velmurugan S. Green synthesis, characterization and antimicrobial activities of zinc oxide NPs from the leaf extract of Azadirachta indica (L.). Appl Surf Sci 2015;345:329–36. https://doi.org/10.1016/j.apsusc.2015.03.176
54. Alharbi NS, Alsubhi NS, Felimban AI. Green synthesis of silver NPss using medicinal plants: characterization and application. J Radiat Res Appl Sci 2022;15:109–24. https://doi.org/10.1016/j.jrras.2022.06.012
55. Veena M, Puthur JT. Seed nutripriming with zinc is an apt tool to alleviate malnutrition. Environ Geochem Health 2022;44:2355–73. https://doi.org/10.1007/s10653-021-01054-2
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