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Volume: 6, Issue: 4, July-August, 2018
DOI: 10.7324/JABB.2018.60405

Research Article

Comparative analyses of genomic DNA extracted from freshwater fish tissues preserved in formaldehyde and alcohol in different periods of time

R. K. Garg, Khushboo Sengar, R. K. Singh

  Author Affiliations


Abstract

High yields of extracted DNA from animal tissues for a number of molecular research work are depended on acquiring tissues that should not highly degraded and must give a sufficient yield of DNA. Current experiments were performed on various tissues preservations, so, later on, the DNA extraction can be done to get high quality and quantity of DNA. 90% alcohol, 8% formalin, and −40°C deep freezing were used for 5 days, 20 months, and 28 months each to stay fish tissues. DNA was extracted from preserved tissues after specified intervals; quantification was performed using Nanodrop spectrophotometer and polymerase chain reaction amplifications through random primers. Genomic DNA extraction method was used for good quantity and reproducibility for molecular markers studies in Sperata seenghala population. Phenol-chloroform-isoamyl alcohol method agreed clear, reproducible, and high quality of bands preserved in 90% alcohol and the purity near was 1.8. However, formalin-preserved samples gave the low quantity of DNA as 8.0 ng/μl with 1.35 ratios was not acceptable for molecular work. Two methods proved successful results as “tissues was preserved in 90% alcohol” and “−40°C preserved in Deep Freezer,” whereas formalin-based preservation method failed with respect to molecular work.

Keywords:

Genomic DNA extraction, Chloroform-isoamyl-alcohol, Molecular markers, Tissues preservatives, DNA integrity.



Citation: Garg RK, Sengar K, Singh RK. Comparative analyses of genomic DNA extracted from freshwater fish tissues preserved in formaldehyde and alcohol in different periods of time. J App Biol Biotech. 2018;6(04):26-31. DOI: 10.7324/JABB.2018.60405


Copyright: Author(s). This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License, which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited.

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