Research Article | Volume: 3, Issue: 5, Sep-Oct, 2015

An insight into wheat haploid production using wheat x maize wide hybridization

Anjanabha Bhattacharya Bhavesh Palan Bharat Char   

Open Access   

Published:  Oct 22, 2015

DOI: 10.7324/JABB.2015.3509

The present study was carried out with six wheat and four maize genotypes from Mahyco Research Centre, Dawalwadi, Jalna, MH to develop wheat double haploid lines by wheat x maize crossing system. The following steps were followed while conducting the experiment: emasculation of wheat spikes, crossing or pollination of emasculated spikes with maize pollens, treating pollinated spikes with 100 ppm 2,4-D solution, excision of embryo and subsequent culture on half strength MS media. In total, 296 spikes were emasculated and pollinated with randomly collected maize pollens after 24 hrs. Success was obtained in rescuing a total of 27 embryos from 575 caryopses among two genotypes i.e. MW2 and MW3. Percentage of embryo formation varied in the range of 0-10 per cent owing to non-standard conditions. However, the frequency of embryo formation could be increased by focusing on the most important of all culture conditions i.e. temperature (18-21 °C).

Keyword:     Wheat maize haploid wheat x maize cross system 2 4-D.


Anjanabha Bhattacharya, Bhavesh Palan, Bharat Char. An insight into wheat haploid production using wheat x maize wide hybridization. J App Biol Biotech. 2015; 3 (05): 044-047. DOI: 10.7324/JABB.2015.3509

Copyright: Author(s). This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.

HTML Full Text

1. Grauda D, Lepse N, Strazdina V, Kokina I, Lapina L, Mikelsone A, Lubinskis L, Rashal I. Obtaining of doubled haploid lines by anther culture method for the Latvian wheat breeding. Agronomy Research (Special Issue III). 2010; 8: 545-552.

2. Lashermes P. Improved anther culture method for obtaining direct regeneration in wheat (Triticum aestivum L.). Journal of Genetics and Breeding. 1992; 46: 99-102.

3. Saeed NA, Chowdhry MA, Khan IA. Induction of callus and organogenesis in bread wheat through anther culture. Pakistan Journal of Agriculture Research. 1994; 15(1): 108-114.

4. Lantos C, Paricsi S, Zofajova A, Weyen J, Pauk J. Isolated microspore culture of wheat (Triticum aestivum L.) with Hungarian cultivars. Acta Biologica Szegediensis. 2006; 50(1-2): 31-35.

5. Liu W, Zheng MY, Konzac CF. Improving green plant production via isolated microspore culture in bread wheat (Triticum aestivum L.). Plant Cell Report. 2002; 20: 821-824.

6. Shahinul Islam SM. The effect of colchicine pretreatment on isolated microspore culture of wheat (Triticum aestivum L.). Australian Journal of Crop Science. 2010; 4(9): 660-665.

7. Zheng MY. Microspore culture in wheat (Triticum aestivum)-doubled haploid production via induced embryogenesis. Plant Cell, Tissue and Organ Culture. 2003; 73: 213-230.

8. Campbell AW. Assessment of wheat x maize doubled haploid technology for genetic improvement of New Zealand wheat. A Ph.D. thesis submitted to Lincoln University, Canterbury, New Zealand. 1999.

9. Khan MA, Ahmad J. In vitro wheat haploid embryo production by wheat x maize cross system under different environmental conditions. Pakistan Journal of Agriculture Sciences. 2011; 48(1): 49-53.

10. Niroula RK, Bimb HP, Thapa DB, Sah BP, Nayak S. Production of haploid wheat plants from wheat (Triticum aestivum L.) x maize (Zea mays L.) cross system. Himalayan Journal of Sciences. 2007; 4(6): 65-69.

11. Inagaki M, Mujeeb-Kazi A. Comparison of polyhaploid production frequencies in crosses of hexaploid wheat with maize, pearl millet and sorghum. Breeding Science. 1995; 45: 157-161.

12. Inagaki MN, Tahir M. Production of haploid wheat through intergeneric crosses. Hereditas. 1992; 116: 117-120.

13. Garcia-Llamas C, Ramirez MC, Ballesteros J. Effect of pollinator on haploid production in durum wheat crossed with maize and pearl millet. Plant Breeding. 2004; 123: 201-203.

14. Laurie DA, Bennett MD. Wheat x maize hybridization. Canadian Journal of Genetics and Cytology. 1986; 28: 313-316.

15. Moradi P, Haghnazari A, Bozorgipour R, Sharma B. Development of yellow rust resistant doubled haploid lines of wheat through wheat x maize crosses. International Journal of Plant Production. 2009; 3(3): 77-88.

16. Khan MA, Shaukat S, Ahmad J, Kashif M, Khan AS, Iqbal MZ. Use of intergeneric cross for production of doubled haploid wheat (Triticum aestivum L.). Journal of Science, Technology and Development. 2012; 31(4): 295-300.

17. Niroula RK, Bimb HP. Overview of wheat x maize system of crosses for dihaploid induction in wheat. World Applied Sciences Journal. 2009; 7(8): 1037-1045.

18. Laurie DA, Bennett MD. The production of haploid wheat plants from wheat x maize crosses. Theoretical and Applied Genetics. 1988; 76: 393-397.

19. Ahmad J, Chowdhry MA. The efficiency of chromosome doubling in haploids derived from wheat x maize crossing. Pakistan Journal of Biological Science. 2005; 8(12): 1707-1711.

20. Niroula RK, Thapa DB. Response of Wheat Genotypes to Maize Mediated Polyhaploid Production. American-Eurasian Journal of Agronomy. 2009; 2 (3): 156-161.

21. Torres LE, Bima P, Maich R, Badiali OJJ, Nisi J. Production of haploid plants from ten hybrids of bread wheat (Triticum aestivum L.) through wide hybridization with maize (Zea mays L.). Agriscientia. 2010; Vol. XXVII (2): 79-85.

Article Metrics

154 Absract views 181 PDF Downloads 335 Total views

Related Search

By author names

Citiaion Alert By Google Scholar

Similar Articles

Agronomic evaluation of temperate maize populations in tropical zone

Justin Abadassi

Bioprospecting of endophytic bacteria from the Indian Himalayas and their role in plant growth promotion of maize (Zea mays L.)

Kusam Lata Rana, Divjot Kour, Tanvir Kaur, Rubee Devi, Ashok Yadav, Ajar Nath Yadav

Upsurge production of cellulase from maize stover under soildstate conditions mediated by Streptomyces enissocaesilis DQ026641

Ashok Sudarshan, Siddanna Renuka, Sirasagar Reshma, Bhalerao Shilanjali, Dayanand Agsar

Accumulation of cadmium in maize roots inoculated with root organ culture of Rhizophagus irregularis improving cadmium tolerance through activation of antioxidative defense enzymes

Rajamony VinuRadha, Karunanandham Kumutha, Asish Kanagaraj Binodh

Effect of growth regulators on callus morphology of Rice anther culturec

Avinash Sharma, Dalpat Lal and Monoj Sutradhar

Exploring factors affecting anther culture in rice (Oryza sativa L.)

Swapan Kumar Tripathy, D. Swain, P. M. Mohapatra, Arjun M. Prusti, Bandita Sahoo, Sucharita Panda, Monalisha Dash, Bhaskar Chakma, Suraj K. Behera

Frequencies of Insulin- Promoter Factor-I (IPF-I) gene mutations in a Cohort of Sudanese Patients with Type 2 Diabetes Mellitus

Nahla A Elsheikh and Eltahir AG Khalil

Meta-analysis of variant rs 7903146 of TCF7L2 gene with secondary complications of Type 2 Diabetes Mellitus

Kanhaiya Singh, NK Agrawal, SK Gupta, Kiran Singh

Humoral immune response in cattle and buffaloes vaccinated against Pasteurella multocida B: 2 with reference to levels of B Lymphocytes and IL-2R+

Harsh Shaveta, Deepti Chachra, H M Saxena, Mudit Chandra

Comparative analysis of two catalytically distinct endoglucanases from Aspergillus nidulans

Baljit Kaur, H.S. Oberoi, B.S. Chadha

Insight into interplay of some molecular chaperones in a hill stream teleost, Barilius bendelisis under wide seasonal and diurnal thermal fluctuations

Saurabh Dewan,Yashpal Singh,JP Bhatt

Cloning, expression and purification of resistance gene analogue RGPM 301 from pearl millet in Escherichia coli

Mariswamy Veena, Prasad Melvin, Sekhar Shailasree, Kukkundoor Ramachandra Kini

Growth, physiological and biochemical responses of Meliaceae species - Azadirachta indica and Melia dubia to elevated CO2 concentrations

S. Janani, P. Priyadharshini, R. S. C. Jayaraj, C. Buvaneswaran, Rekha R. Warrier

Effects of Malaria in Pregnancy (MiP) on Pregnancy Development and its Outcome: a Critical Review

Joshua Djabanor, Elvis Quansah, Du-Bois Asante

In-Vitro Propagation of Dioscorea alata for Tyrosinase Production

B. Gopal Samy, K. Jegatheesan, C. Infant Francina

In-vitro Assessment of Carbendazim and Copper oxychloride cytotoxicity on HaCaT and HepG2 human cell lines

Diksha Sateesh Bakre, Basappa Basawanneppa Kaliwal

Aroclor 1254 induced oxidative stress and histopathological changes in mice liver

Jalpa Raja, Shweta Pathak, Rahul Kundu

Cytotoxicity and antiproliferative effects of ethyl acetate fraction of Padina australis against MCM-B2 and K562 cell lines.

Heder Djamaludin, Maria Bintang, Bambang Pontjo Priosoeryanto

Low dose and subchronic Aroclor 1254 induced histopathological changes in mice kidney

Jalpa Raja, Shweta Pathak, Rahul Kundu

Study of the changes in the growth, protein, and bioactive profile of Chlorella emersonii KJ725233 in response to sodium and ammonium nitrate

Sneha Sunil Sawant, Varsha Kelkar-Mane

Study of impact of Wolbachia surface protein on reactive oxygen species production in HepG2 cell line exposed to ethanol

V. P. Kishore, H. P. Puttaraju

Mycelial biomass, antioxidant, and myco-actives of mycelia of abalone mushroom Pleurotus cystidiosus in liquid culture

Kent Garcia, Chester Jhae Garcia, Reynante Bustillos, Rich Milton Dulay

Detection of 3243 A/G and 3316 G/A mitochondrial DNA mutations in Nagpur population

Utpal Jagdish Dongre, Virendra Govindrao Meshram

Detection of SARS-CoV-2 using high-throughput PCR

Saakshi Jalali, Vikas K. Patel, Venkatesh Prasad, Ajit Sapre, Santanu Dasgupta, Bhaskar Bhadra

In vitro antioxidant activity of Lactobacillus plantarum against hydrogen peroxide-induced neuronal damage on PC12 cells

Shani Kunjamma John, Vani Chandrapragasam

A novel strategy for disarming quorum sensing in Pseudomonas aeruginosa-Chlorella emersonii KJ725233

Sneha Sawant Desai, Reema Devi Singh, Sukhendu B Ghosh, Varsha Kelkar

Recent advances in the knowledge of coronaviruses with special emphasis on severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)

Khushboo Irshad, Madeeha Mudassir, Sheeba Irshad, Mohammad Irshad

Statistical optimization of chitinase production by Box–Behnken design in submerged fermentation using Bacillus cereus GS02

Garima Dukariya, Anil Kumar

Enhancement of Vitamin D2 content through ultraviolet-B irradiation in submerged cultivated Pleurotus eryngii mycelia using response surface methodology

Umesh Singh, Ashwani Gautam, Satyawati Sharma

Modification of the time of incubation in colorimetric method for accurate determination of the total antioxidants capacity using 2,2-diphenyl-1-picrylhydrazyl stable free radical

Abhipsa Bal, Samar Gourav Pati, Falguni Panda, Biswaranjan Paital

The linkage between the second wave of COVID-19 and the severity of mucormycosis in India

Kshama Wamanrao Murarkar, Shilpa Prakash Mankar

Augmentative role of Piriformospora indica fungus and plant growth promoting bacteria in mitigating salinity stress in Trigonella foenum-graecum

Sanskriti Bisht, Shatrupa Singh, Madhulika Singh,, Jai Gopal Sharma

The ITS2 DNA sequence analysis in six species of barbin fishes with phylogenetic insights

Sandip Choudhury, Briyanka Kashyap, Karabi Dutta

Diversity due to mutations in circulating virus strains of SARS-CoV-2 may delay control of COVID-19

Sharanagouda S. Patil, Chandan Shivamallu, Chandan Dharmashekara, Sushma Pradeep, Kuralayanapalya Puttahonnappa Suresh, Ashwini Prasad, Shiva Prasad Kollur, Mahendra P. Yadav, Chandrashekar Srinivasa, Bramhadev Pattnaik

Genetic polymorphism of soybean genotypes with contrasting levels of phosphatidylcholine, protein, and lipoxygenase-2

Aseem Kumar Anshu,, Trupti Tayalkar, Anita Rani, Vineet Kumar, Hamendra Singh Parmar

Cloning the root-specific Asy promoter and genes encoding chitinase 42 kDa of Trichoderma asperellum into the plant expression vector

Nguyen Hoang Tue, Tran Gia Cat Tuong, Pham Thi Huyen Trang, Nguyen Duc Chung, Phung Thi Bich Hoa,, Nguyen Quang Duc Tien, Nguyen Hoang Loc

Seed treatment with 24-epibrassinolide enhances soybean seed germination under salinity stress

Victoria Oko Otie, Idorenyin Udo, Shuoshuo Liang, Shao Yang, Michael Itam, Ping An, Egrinya Anthony Eneji

Cowpea (Vigna unguiculata L. Walp.): A choice crop for sustainability during the climate change periods

Tewodros Ayalew, Tarekegn Yoseph

Antifungal effects of Kurthia gibsonii Mb 126 chitinase as a seed treatment on seed-borne fungi of rice seed on germination percentage and seedling vigor

Mini K. Paul, K. D. Mini, Jyothis Mathew

Health-related risk of SARS-CoV-2 infection in chronic obstructive pulmonary disease patients: A systematic review

Meenakshi S, Raghunath N, Vishal B Rawal, Ramith Ramu

Meta-analysis of Type 2 diabetes and insulin resistance gene expression datasets to decipher their associated pathways

Aditya Saxena, Nitish Mathur, Uma Chaudhary, Utkarsh Raj, Sneha Rai, Sandeep Kumar Mathur

A subchronic toxicity test of a combination of n-hexane and ethyl acetate fraction of rose myrtle leaves (Rhodomyrtus tomentosa [Ait.] Hassk) on male white rats

Salni Salni, Herlina Herlina, Aprila Purnamasari

Designing of guide RNA constructs for CRISPR/Cas9-mediated editing of rice transcription factor OsMADS26 for enhancing drought tolerance

K. Anjala, Rehna Augustine

Humoral immune response in COVID-19 patients and novel design of lateral flow assay strip for simultaneous rapid detection of IgA/IgM/ IgG antibodies against the SARS-CoV-2 virus

Huong Thu Ngo, Son Van Tran, Hien Dang Nguyen, Phong Quoc Truong

Isolation and characterization of robust plant growth-promoting rhizobacteria from lignite mines, Gujarat

Ravi Patel, Dilip N. Borada, Amisha Patel, Neil J. Shah

In-vitro and in-vivo models for the identification and validation of radioprotectors and radiosensitizers

Debasish Mohapatra,, Amlan Priyadarshee Mohapatra,, Anjan Kumar Sahoo, Shantibhusan Senapati

Begomovirus detection in the whitefly Bemisia spp. on eggplant Solanum melongena L. leaves

Rachmi Putri, Prasetya Anugerah Gusti, Nastiti Wijayanti

Evaluation of functional characteristics of roselle seed and its use as a partial replacement of wheat flour in soft bread making

Nguyen Minh Thuy, Nguyen Bao Tram, Dinh Gia Cuong, Huynh Khanh Duy, Ly Thanh An, Vo Quoc Tien, Tran Ngoc Giau, Ngo Van Tai

Development of a colloidal Gold nanoparticle-based lateral-flow assay for rapid detection of SARS-CoV-2 showing enhanced sensitivity and specificity

Suresh Singh, Tapan Singha, Rajat Maheshwari, Pankaj Yadav, Arun Kumar, Harendra Bhoj, Bharti Sharma, Amit Verma, Amit Gupta, Harsh Singh, Sonal Mishra, Rajeshwar Sinha

Effects of gibberellic acid on seed dormancy of black gram (Vigna mungo L.)

Vinothini Nedunchezhiyan, Mohanasundari Palanivel, P. A. Akhila Jabeen, Poovarasan Thangavel, Bhavyasree Ramakrishnan, Manonmani Velusamy, Sakila Muthusamy, Isong Abasianyanga Edm

Neutrophil gelatinase-associated lipocalin a proinflammatory polypeptide necessary for host cell survival in bacterial infection

Nichita Yadav Aare, Pawan Kumar Anoor, Swathi Raju M, N. Srinivas Naik, Sandeepta Burgula

Production of SARS-CoV-2 nucleocapsid protein in Escherichia coli and its characterization

Vaishali Verma, Abhishek

Identification of matrix metalloproteinases inhibitors from methanol extract of Peltophorum pterocarpum leaves

Bhimrao V. Jaiwal, Faiyaz K. Shaikh, Sarwan W. Bradosty, Ajit B. Patil, Raghavendra L. S. Hallur

Sustainable biodegradation of textile dye reactive blue 222 by the novel strain Enterobacter CU2004, isolated from the industrial waste: A design of experiment based optimization study and characterisation of metabolites

Vasantha Veerappa Lakshmaiah, Anish Nag, Suresh Gotekar, Sunil More, Shobha K. Jayanna

Isolation and Characterization of Cellulase-Producing Myxobacterial Strain from the Unique Niche of Mirgund Wetland from the North-Western Himalayas

Daljeet Singh Dhanjal, Simranjeet Singh, Vijay Kumar, Praveen C. Ramamurthy, Chirag Chopra, Atif Khurshid Wani, Reena Singh, Joginder Singh

A novel trypsin inhibitor peptide MoCh I with antimicrobial activity derived from Momordica charantia

Samriti Dogra, Rajesh Biswas, Rupinderjeet Kaur, Sangeeta Sharma, Kakoli Biswas

Isolation of toxic gas-producing bacteria (Desulfovibrio spp.) from shrimp ponds and potential of bacteriophages as biocontrol

Truong Thi Bich Van, Tran Vo Minh Thu

Novel use of probiotic as acetylcholine esterase inhibitor and a new strategy for activity optimization as a biotherapeutic agent

Abdulrahman M. Qadah, Amr A. El-Waseif, Heba Yehia

Evaluation and comparison of 15 short tandem repeat loci of south and west Indian population for use in personal identification applications

Prabakaran Mathiyazhagan, Thangaraju Palanimuthu, Agasthi Padmanathan

Effect of phenolic disinfectant on sporulation inhibition of Eimeria tenella for prevention of coccidiosis

Rajendra Moorthy Rajendran, Nabila Fatima S

In silico screening and analysis of candidate microRNA-target interaction involved in progression of prediabetes to Type II Diabetes Mellitus

Angel Mendonca, Prabu Thandapani, Priya Swaminathan, Sujatha Sundaresan

Sugarcane yield prediction using NOA-based swin transformer model in IoT smart agriculture

V. Gokula Krishnan, B. V. Subba Rao, J. Rajendra Prasad, P. Pushpa, S. Kumari

Role of DREB genes in the regulation of salt stress-mediated defense responses in plants

Ashokkumar Ramakrishnan Yadav, Vaishnavi Ashokkumar, Suganthi Muthusamy, Senthilkumar Palanisamy

Rapid and visual detection of leptospira interrogans using polymerase spiral reaction assay

Archana Vishwakarma, Punith Chowdary, Mohandass Ramya

Purification and structural characterization of Chojalactone C, a promising anticancer compound from a marine bacterium, Paenibacillus lentimorbus SAGM 3

Nagamuthu Vinothkumar, Pachaiappan Pugalendhi

Combinatorial network of transcriptional and post-transcriptional regulation in amyotrophic lateral sclerosis

Rupesh Kumar, Pammi Gauba, Shazia Haider

Phytochemicals of Nardostachys jatamansi as potential inhibitors of HCV E2 receptor: An in silico study

Lalrintluanga Hnamte, Praveen Nagella, Aatika Nizam, Vasantha Veerappa Lakshmaiah