Development of a colloidal Gold nanoparticle-based lateral-flow assay for rapid detection of SARS-CoV-2 showing enhanced sensitivity and specificity

Suresh Singh Tapan Singha Rajat Maheshwari Pankaj Yadav Arun Kumar Harendra Bhoj Bharti Sharma Amit Verma Amit Gupta Harsh Singh Sonal Mishra Rajeshwar Sinha   

Open Access   

Published:  Nov 11, 2022

DOI: 10.7324/JABB.2023.110228

To stop the spread of COVID-19 in this outbreak, diagnostic testing is essential. Quick diagnostic tests must be employed in this pandemic, which is brought on by the severe acute respiratory syndrome (SARS)-CoV-2 virus, to successfully treat and manage COVID-19. There are several problems with the present RT-PCR system that the lateral flow assay (LFA), a kind of clinically sensitive diagnostic test, may be able to fix, especially in low- and middle-income nations. Gold nanoparticle- (AuNP-LFA) is a practical method for detecting COVID-19 in basic hospitals and laboratories, particularly in emergency situations where many samples must be quickly examined. Safe, accurate, and non-toxic diagnostic tests must be employed during the pandemic, to successfully treat and manage COVID-19. Recombinant SARS-CoV-2 nucleocapsid monoclonal antibody was employed to detect COVID-19 antigens in the presence of patients to establish a fast LFA for COVID-19. Synthesis of colloidal gold particles and antibody colloidal gold conjugates was evaluated by using UV/Vis spectroscopy. A capture line made of anti-SARS-CoV-2 antibody was coated on nitrocellulose membrane. To create the control line, goat anti-mouse IgG monoclonal antibody was coated. On a polystyrene backing board, the immunochromatographic strip was constructed in the ideal order. Using ELISA as the standard procedure, the strips’ sensitivity and specificity were assessed. The results’ stability and repeatability were evaluated over a 9-month period. Colloidal gold nanoparticle-based LFAs created in this study can be employed for quicker and more accurate detection of SARS-CoV-2.

Keyword:     Gold nanoparticle Monoclonal antibody COVID-19 SARS-CoV-2 Lateral flow assay


Singh S, Singha T, Maheshwari R, Yadav P, Kumar A, Bhoj H, Sharma B, Verma A, Gupta A, Singh H, Mishra S, Sinha R. Development of colloidal gold nanoparticle-based lateral-flow assay for rapid detection of SARS-CoV-2 showing enhanced sensitivity and specificity. J App Biol Biotech. 2022.

Copyright: Author(s). This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.

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1. Mindray Diagnostics List of Selected Chinese IVD Manufacturers. Vol. 28. IVD Industry in China, Report; 2021. p. 276.

2. Lombardi AF, Afsahi AM, Gupta A, Gholamrezanezhad A. Severe acute respiratory syndrome (SARS), middle East respiratory syndrome (MERS), influenza, and COVID-19, beyond the lungs: A review article. Radiol Med 2021;126:561-9.

3. Hsieh WY, Lin CH, Lin TC, Lin CH, Chang HF, Tsai CH, et al. Development and efficacy of lateral flow point-of-care testing devices for rapid and mass COVID-19 diagnosis by the detections of SARS-CoV-2 antigen and anti-SARS-CoV-2 antibodies. Diagnostics (Basel) 2021;11:1760.

4. Kevadiya BD, Machhi J, Herskovitz J, Oleynikov MD, Blomberg WR, Bajwa N, et al. Diagnostics for SARS-CoV-2 infections. Nat Mater 2021;20:593-605.

5. Pan Y, Zhang D, Yang P, Poon LL, Wang Q. Viral load of SARS-CoV-2 in clinical samples. Lancet Infect Dis 2020;20:411-2.

6. Yang Y, Yang M, Shen C, Wang F, Yuan J, Li J, et al. Laboratory diagnosis and monitoring the viral shedding of 2019-nCoV infections. Innovation (Camb) 2020;1:10061.

7. Ai T, Yang Z, Hou H, Zhan C, Chen C, Lv W, et al. Correlation of chest CT and RT-PCR testing in coronavirus disease 2019 (COVID-19) in China: A report of 1014 cases. Radiology 2020;296:E32-40.

8. Winichakoon P, Chaiwarith R, Liwsrisakun C, Salee P, Goonna A, Limsukon A, et al. Negative nasopharyngeal and oropharyngeal swabs do not rule out COVID-19. J Clin Microbiol 2020;58:e00297-20.

9. Poller B, Strachan C, Broadbent R, Walker GF. A mini tablet formulation made from electrospun nanofibers. Eur J Pharm Biopharm 2017;114:213-20.

10. European Commission. "Press Corner". Belgium: European Commission; 2021.

11. Khatoon UT, Rao GN, Mohan KM, Ramanaviciene A, Ramanavicius A. Antibacterial and antifungal activity of silver nanospheres synthesized by tri-sodium citrate assisted chemical approach. Vacuum 2017;146:259-65.

12. Dong J, Carpinone PL, Pyrgiotakis G, Demokritou P, Moudgil BM. Synthesis of precision gold nanoparticles using Turkevich method. Kona 2020;37:224-32.

13. Deb R, Pal P, Chaudhary P, Bhadsavle S, Behera M, Gautam D, et al. Development of gold nanoparticle-based visual assay for rapid detection of Escherichia coli specific DNA in milk of cows affected with mastitis. LW Food Sci Technol 2022;155:112901.

14. Schadler J, Sigrist B, Meier SM, Albini S, Wolfrum N. Virus-like particles in a new vaccination approach against infectious laryngotracheitis. J Gen Virol 2019;100:1013-26.

15. Yu M, Bao Y, Wang M, Zhu H, Wang X, Xing L, et al. Development and application of a colloidal gold test strip for detection of avian leukosis virus. Appl Microbiol Biotechnol 2019;103:427-35.

16. Maya V, Kaviyil JE, Ponnambath DK, Nair RP, Bhatt A, Pilankatta R, et al. Evaluation of diagnostic accuracy of developed rapid SARS-CoV-2 IgG antibody test kit using novel diluent system. Virusdisease 2021;32:78-84.

17. Haiss W, Thanh NT, Aveyard J, Fernig DG. Determination of size and concentration of gold nanoparticles from UV-Vis spectra. Anal Chem 2007;79:4215-21.

18. Huang C, Wen T, Shi FJ, Zeng XY, Jiao YJ. Rapid detection of IgM antibodies against the SARS-CoV-2 virus via colloidal gold nanoparticle-based lateral-flow assay. ACS Omega 2020;5:12550-6.

19. Zhang Y, Xiao W, Kong H, Cheng J, Yan X, Zhang M, et al. A highly sensitive immunochromatographic strip test for rapid and quantitative detection of Saikosaponin D. Molecules 2018;23:338.

20. Shen B, Zheng Y, Zhang X, Zhang W, Wang D, Jin J, et al. Clinical evaluation of a rapid colloidal gold immunochromatography assay for SARS-CoV-2 IgM/IgG. Am J Transl Res 2020;12:1348-54.

21. Kirkpatrick B, Fenton WS, Carpenter WT, Marder SR. The NIMH-MATRICS consensus statement on negative symptoms. Schizophr Bull 2006;32:214-9.

22. Kasem S, Qasim I, Al-Doweriej A, Hashim O, Alkarar A, Abu-Obeida A, et al. The prevalence of middle east respiratory syndrome coronavirus (MERS-CoV) infection in livestock and temporal relation to locations and seasons. J Infect Public Health 2018;11:884-8. 23. Nasajpour M, Pouriyeh S, Parizi RM, Dorodchi M, Valero M, Arabnia HR. Internet of things for current COVID-19 and future pandemics: An exploratory study. J Healthc Inform Res 2020;4:325-64.

24. Zhai X, Mei X, Wu X, Zuo L, Zhou L, Tian Y, et al. A loop-mediated isothermal amplification coupling with a lateral flow dipstick for rapid and specific detection of fowl adenovirus serotype-4. J Virol Methods 2019;270:79-86.

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