Published:  Oct 22, 2015DOI: 10.7324/JABB.2015.3504
Soil samples were collected from garden of P.G. Department of Botany, Utkal University, Odisha India. The physical analysis of soil revealed that texture of the soil was sandy loam with pH 5.7, temperature 34.6ºC, 5.7% water content and 67.3% water holding capacity, respectively. Utmost numbers of Aspergillus species with other fungi were isolated from garden soil (5×105 CFU/g). Total 19 fungal taxa were isolated from all the samples studied. The isolated species were belonging to 8 genera representing the genus Aspergillus as dominant one. Total twelve fungal species of six genera were recorded from air of the pesticide treated soil area. Soil treated with 600ppm cypermethrin exhibited ten fungal species. Out of which, A. flavus appeared in greater number as compared to other fungi. Similarly, A. candidus was contributed highest percentage followed by A. flavus in dilution plate technique. Total eight species of fungi belonging to three genera were found when soil was treated with cypermethrin at a concentration of 800 ppm. A. candidus and A. flavus were isolated in highest number at 1000ppm cypermethrin. But, in direct plate technique, A. flavus was found to be the dominant species followed by A. terreus and A. niger at 1000 and 1200ppm concentration of cypermethrin, respectively. Soil treated with cypermethrin at 1400ppm concentration exhibited that A. awamori was the dominant species isolated followed by A. terreus and Mucor hiemalis both in direct and dilution plate techniques. Among all the isolates screened for protease activity, A. terreus was potent enough for this production.
Bijay Kumar Sethi, Subhashree Pradhan, Narayan Behera and Santi Lata Sahoo. Effect of cypermethrin, a pyrethroid insecticide on dynamics of soil microflora. J App Biol Biotech. 2015; 3 (05): 019-025. DOI: 10.7324/JABB.2015.3504
1. Voets J.P., Meerschman P., Verstraete W. Soil microbiological and biochemical effects of long-term atrazine applicationsSoil Biology and Biochemistry. 1974, 6, 149-152.
2. Duah-Yentumi S., Johnson D.B. Changes in soil microflora in response to repeated applications of some pesticides. Soil Biology and Biochemistry. 1986, 18, 629-635.
3. Rai, J.P.N. Effect of long-term 2,4-D application on soil microbial populations. Biology of Fertile Soils. 1992, 13, 427-431.
4. Dumontet S., Perucci P. The effect of acifluorfen and trifluralin on the size of microbial biomass in soil. The Science and Total Environment. 1992, 123-124.
5. Megharaj M. Kondinin Landcare Group Magazine, Farming Ahead, 2002, 121, 37-38.
6. Smith M.D., Hartnett D.C., Rice C.W. Effects of long-term fungicide applications on microbial properties in tallgrass prairie soil. Soil Biology and Biochemistry. 2000, 32, 935-946.
7. Pankhurst C.E., Doube B.M., Gupta V.V.S.R. Biological Indicators of Soil, edn. 1997.
8. Sannino F., Gianfreda L., Chemosphere. Pesticide influence on soil enzymatic activities. Chemosphere. 2001, 45, 417-425.
9. Johnsen K., Jacobsen C.S., Torsvik V. Pesticide effects on bacterial diversity in agricultural soils-A review. Biology of Fertile Soils. 2001, 33, 443-453.
10. Doran J.W., Parkin T.B. Defining and assessing soil quality. In: JW Doran, DC Coleman, DF Bezdicek and BA Stewart (ed.) Defining Soil Quality for a Sustainable Environment. Soil Science Society of America Special Publication, 1994, 35, pp 3-21.
11. Gregorich E.G., Carter M.R., Doran J.W., Pankhurst C.E., Dwyer L.M. Biological attributes of soil quality. In: EG Gregorich, and Carter MR. (ed,) Soil Quality for Crop Production and Ecosystem Health. Elsevier, 1997, pp. 81-113.
12. Sethi B.K., Nanda P.K., Sahoo S.L. Isolation, Identification And Conservation Of Potent Hydrolase Producer From Different Soils Of Odisha, India. International Journal of Pharma and Bio Sciences. 2013, 4(2)(B), 89-100.
13. Alexopoulos C.J., Mims C.W. Introductory Mycology. 3rd ed., John Wiley and Sons, New York, 1979.
14. Watanabe T. Pictorial Atlas of Soil and Seed Fungi. 2nd Ed., CRC Press, London, 2002.
15. Hankin L., Anagnostakis S.L. The use of solid media for detection of enzyme production by fungi. Mycologia. 1975, 67(3), 597-607.
16. Grant W.D., Mwatha W.E., Jones B.E. Alkaliphiles: ecology, diversity and applications. FEMS Microbiology Review. 1990, 75, 255-70.
17. Fleet G.H., Mian M.A. The occurrence and growth of yeasts in dairy products. Internet Journal of Food Microbiology. 1987, 4, 145-155.
18. Parkinson D., Gray T.R.G., William S.T. Methods for studying the Ecology of soil microorganisms. International Biological Programme Hand book No-19. Black well Scientific Publications, Oxford, 1971.
19. Chakraverty R., Sinha S. Grana. The Incidence of Aspergillus Parasiticus in the Indoor and Outdoor Environments of Calcutta, India. Grana. 1985, 24(2), 133-135.
20. Beg K.Q., Gupta R. Purification and characterization of an oxidation-stable, thiol-dependent serine alkaline protease from Bacillus mojavensis. Enzyme and Microbial Technology. 2003, 32, 294-304.
21. Ali U.F. Utilization of whey amended with some agro-industrial by-products for the improvement of protease production of Aspergillus terreus and its compability with commercial detergents. Research Journal of Agriculture and Biological Science. 2008, 4(6), 886-891.
22. Negi S., Banerjee R. Characterization of amylase and protease produced byAspergillus awamori in a single bioreactor Food Research International. 2009, 42, 443-448.
23. Saravanakumar K., Thiyagarajan A., Kaviyarasan V. Optimization of medium constituents for the production of extra cellular alkaline Protease by As p e rg illus fis c h e ri using response surface experimental designJournal of Bioscience Research. 2010, 1(3), 118-129.
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