Research Article | Volume: 3, Issue: 5, Sep-Oct, 2015
Screening and evaluation of non-heterocystous filamentous cyanobacteria for lipid and commercially viable fatty acids
Thirty eight unialgal non-heterocystous filamentous cyanobacteria were isolated from rice fields of Manipur, India; cultured as unialgal, deposited to the national repository of cyanobacteria and microalgae and obtained accession number. All these strains were screened and investigated for the production of total lipid and commercially viable fatty acids in culture condition. Equal amount of total lipid (3%) was produced by Limnothrix vacuolifera BTA05, Plectonema boryanum BTA16, Plectonema nostocorum BTA47, Lyngbya laxespiralis BTA85 and Lyngbya norgardhii BTA184 in exponential growth phase. The commercially viable fatty acids, namely; palmitic acid (C16:0), palmitoleic acid (C16:1), oleic acid (C18:1n9c), linoleic acid (C18:2n6c) and γ- linolenic acid (C18:3n6) were focused in present study. The investigation revealed that Plectonema notatum BTA88 yielded high content of palmitic acid (27.9%); Oscillatoria agardhii BTA170 of palmitoleic acid (8.90%); Lyngbya martensiana BTA640 of oleic acid (56.2%); Phormidium faveolarum BTA20 (11.8%) in linoleic acid and Phormidium boryanum BTA16 of γ- linolenic acid (8.82%). These organism were considered as the potential candidates for fatty acids profiling, however palmitic acid C16:0 was common and recorded in all 38 examined strains.
How to cite this article: Indrama T., Tiwari O.N., Ojit S.K., Gunapati O., Avijeet S.O., Sarabati K., Bidyababy T., Indira W., Silvia Ch., Subhalaxmi A., Romi K., Thadoi A., Sharma G.D. Screening and evaluation of non-heterocystous filamentous cyanobacteria for lipid and commercially viable fatty acids. J App Biol Biotech. 2015; 3 (05): 011-014. DOI: 10.7324/JABB.2015.3502
1. Li R, Watanabe MM. Fatty acid profiles and their chemotaxonomy in planktonic species of Anabaena (cyanobacteria) with straight trichomes. Photochemistry. 2001; 57:727-731.
2. Kenyon CN. Fatty acid composition of unicellular strains of blue-green algae. Journal of Bacteriology. 1972; 109:827-834.
3. Borowitzka MA, Borowitzka LJ. Microalgal biotechnology, Cambridge University Press, Cambridge. 1988; 477; 1988.
4. Parker PL, Van Baalen C, Maurer L. Fatty acids in eleven species of blue-green algae: geochemical significance. Science. 1967; 155:707-708.
5. Holton RW, Blecker HH. Fatty acids in blue-green algae. In: Zaick JE, editor. Properties and products of algae, New York: Plenum; 1972, p. 115-127.
6. Kenyon CN, Rippka R, Stanier RY. Fatty acid composition and physiological properties of some filamentous blue green algae. Archives of Mikrobiology. 1972; 83:216-236.
7. Karatay SE, Donmez G. Microbial oil production from thermophile cyanobacteria for biodiesel production. Applied Energy. 2011; 88:3632-3635.
8. Taher H, Al-Zuhair S, Al-Marzouqi AH, Haik Y, Farid MM. A review of enzymatic transesterification of microalgal oil-based biodiesel using supercritical technology. Enzyme Research. 2011; Article ID 468292.
9. Rittmann BE. Opportunities for renewable bioenergy using microorganisms. Biotechnology Bioengineering. 2008; 2:203-212.
10. Caudales R, Wells JM, Butterfield JE. Cellular fatty acid composition of cyanobacteria assigned to subsection II, order Pleurocapsales. International Journal of Systematic Evolutionary Microbiology. 2000; 50:1029-1034.
11. Renaud SM, Thinh LV, Parry DL. The gross composition and fatty acids composition of 18 species of tropical Australian microalgae for possible use in mariculture. Aquaculture. 1999; 170:147-159.
12. Tran H, Hong S, Lee C. Evaluation of extraction methods for recovery of fatty acids from Botryococcus braunii LB 572 and Synechocystis sp. PCC 6803. Biotechnology Bioengineering. 2009; 14:187-192.
13. Volkman JK, Jeffrey SW, Nichols PD, Rogers GI, Garland CD. Fatty acid and lipid composition of 10 species of microalgae used in mariculture. Journal of Experimental Marine Biology and Ecology.1989; 128:219-240.
14. Caudales R, Wells JM. Differentiation of the free-living Anabaena and Nostoc cyanobacteria on the basis of fatty acid composition. International Journal of Systematic Bacteriology.1992; 42:246-251.
15. Kruger GHJ, Wet HD, Kock JLF, Pieterse AJH. Fatty acid composition as taxonomic characteristic for Microcystis and other coccoid cyanobacteria (blue-green alga) isolates. Hydrobiologia. 1995; 308:145-151.
16. Rezanka T, Zahradnik J, Podojil M. Hydrocarbons in green and blue-green algae. Folia Microbiologica. 1982; 27:450-454.
17. Stanier RY, Kunisawa MM, Cohen-Bazire G. Purification and properties of unicellular blue green algae (order Chroococcales). Bacteriology Reviews. 1971; 35:171-201.
18. Bligh EG, Dyer WJ. (1959). A rapid method for total lipid extraction and purification. Canadian Journal of Biochemistry and Physiology. 1957; 37:911-917.
19. Biagi PL, Bordoni A, Hrelia S, Celadon M, Horrobin DF. γ- Linolenic acid dietary supplementation can reverse the aging influence on rat liver microsome 6-desaturase activity. Biochemical Biophysical Acta.1991; 1083:187-192.
20. Ghazala B, Shameel M. Phytochemistry and bioactivity of some freshwater green algae from Pakistan. Pharmaceutical Biology. 2005; 43:358-369.
21. Wainwright PE, Huang YS, Levesque S, Mutsaers L, Mccutcheon D,Balcaen P, Hammond J. Effects of dietary γ- linolenic acid and prenatal ethanol on mouse brain and behavior. Pharmacology Biochemistry Behavior. 1996; 53(4):843-852.
Year
Month