Transaminases activity in the hemolymph: Biomarkers determining the thermal stress in the new bivoltine lines of Bombyx mori.

J. Prashanth H. B. Manjunatha   

Open Access   

Published:  Nov 11, 2022

DOI: 10.7324/JABB.2023.110213
Abstract

Varying atmospheric temperatures invariably induce thermal stress in the silkworm, Bombyx mori, which influence the functionality of aspartate aminotransferase (AST) and alanine aminotransferase (ALT). Thus, we have investigated AST and ALT activity in the new bivoltine lines (NBL). Interestingly, day-3 fifth instar larvae of NBL-5 being more susceptible to the thermal stress of 45°C showed highest level of AST (11.64 μm/mL) and ALT (9.83 μm/mL) activity in their hemolymph. Consequently, on day-5, AST (3.54 μm/mL) and ALT (1.39 μm/mL) activity were lower than day-3. Comparatively, between NBL and its parental breeds, the AST and ALT activity were found to be higher in day-3 (12.41 and 10.90 μm/mL) and day-5 (4.02 and 1.69 μm/mL) larvae of CSR2, while it was 12.93 and 10.14 μm/mL in CSR27 on day-3, and 4.31 and 2.03 μm/mL on day-5, respectively. This salient finding validates the linkage between the rate of cytotoxicity and AST and ALT enzyme activity in relation to the thermotolerance in NBL and its parents. Thus, the silkworm larvae can be considered as a model system for rapid evaluation of cytotoxicity and varied levels of AST and ALT activity as one of the biomarkers to correlate well with the tolerance level to critical temperature.


Keyword:     Bombyx mori Thermal stress Hemolymph Aspartate aminotransferase Alanine aminotransferase


Citation:

Prashanth J, Manjunatha HB. Transaminase activity in the hemolymph: Biomarkers determining the thermal stress in the new bivoltine lines of Bombyx mori. J App Biol Biotech. 2022. https://doi.org/10.7324/JABB.2023.110213

Copyright: Author(s). This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.

HTML Full Text
Reference

1. Oksala NK, Ekmekçi FG, Ozsoy E, Kirankaya S, Kokkola T, Emecen G. Natural thermal adaptation increases heat shock protein levels and decreases oxidative stress. Redox Biol 2014;3:25-8.https://doi.org/10.1016/j.redox.2014.10.003

2. Gething MJ, Sambrook J. Protein folding in the cell. Nature 1992;355:33-45.https://doi.org/10.1038/355033a0

3. Manjunatha HB. Silkworm genomics: Current status and limitations. Adv Anim Genom (Ed. by Sukanta Mondal and Ram Lakhan Singh). 2021; pp 259-280.https://doi.org/10.1016/B978-0-12-820595-2.00016-3

4. Manjunatha HB. Applications of Principles of Heat Shock Response in Silkworm Breeding for the Development of Productive Thermotolerant Strains/Breeds. Trends Adv Sericult Department Sericulture, SK University, Anantapur 515 003, 21-22 January; 2016. p. 10-4.

5. Punyavathi, Manjunatha HB. Comprehensive analysis of differentially expressed proteins in the male and female Bombyx mori larval instars exposed to thermal stress. Arch Insect Biochem Physiol 2020;105:1-16.https://doi.org/10.1002/arch.21719

6. Etebari K, Mirhoseini SZ, Matindoost L. A study on intera specific biodiversity of eight groups of silkworm (Bombyx mori) by biochemical markers. Insect Sci 2005;12:87-94.https://doi.org/10.1111/j.1744-7917.2005.00010.x

7. Huang XJ, Choi YK, Im HS, Yarimaga O, Yoon E, Kim HS. Aspartate aminotransferase (AST/GOT) and alanine aminotransferase (ALT/ GPT) detection techniques. Sensors 2006;6:756-82.https://doi.org/10.3390/s6070756

8. Gowda ML, Manjunatha HB. Streptococcus mutans infection and antibiotic-mediated variation in the alanine aminotransferase and aspartate aminotransferase activity in the silkworm, Bombyx mori. J Emerg Technol Innov Res 2019;6:307-4.

9. Chavadi VB, Sosalegowda AH, Manjunatha HB. Impact of heat shock on heat shock proteins expression, biological and commercial traits of Bombyx mori. Insect Sci 2006;13:243-50.https://doi.org/10.1111/j.1744-7917.2006.00090.x

10. Dandin SB, Jayaswal J, Giridhar K. Handbook of Sericulture Technologies. Bangalore: Central Silk Board, Ministry of Textiles, Govt of India; 2001.

11. Reitman S, Frankel S. A Colorimetric method for the determination of serum glutamic oxalacetic and glutamic pyruvic transaminases.

Am J Clin Pathol 1957;28:56-63.https://doi.org/10.1093/ajcp/28.1.56

12. Prasad SS, Mohan PM. Amino acids, aminotransferases and proteins in the metamorphosing silkworm, Bombyx mori L. Anim Sci 1990;99:369-75.https://doi.org/10.1007/BF03191869

13. Inagaki Y, Matsumoto Y, Kataoka K, Matsuhashi N, Sekimizu K. Evaluation of drug-induced tissue injury by measuring alanine aminotransferase (ALT) activity in silkworm hemolymph. BMC Pharmacol Toxicol 2012;13:13.https://doi.org/10.1186/2050-6511-13-13

14. Reddy KV, Devi O, Magadum SB, Benchamin KV, Datta RK. Uzi parasitisation: gluconeogenic precursorlevels and related enzyme activity profiles in silkworm, Bombyx mori L. Indian J Sericult 1992;31:123-9.

15. Nath BS, Suresh A, Varma BM, Kumar RP. Changes in protein metabolism in hemolymph and fat body of the silkworm, Bombyx mori (Lepidoptera: Bombycidae) in response to organophosphorus insecticides toxicity. Ecotoxicol Environ Saf 1997;36:169-73.https://doi.org/10.1006/eesa.1996.1504

Article Metrics

2 Absract views 7 PDF Downloads 9 Total views

Related Search

By author names

Citiaion Alert By Google Scholar