Research Article | Volume 11, Issue 1, January, 2023

Bioesterification of carboxylic acids by immobilized esterase of Pisum sativum

Rajesh Dattatraya Tak Amol Ashok Bhosale Dnyaneshwar Dashrath Gaikwad   

Open Access   

Published:  Nov 22, 2022

DOI: 10.7324/JABB.2023.110106
Abstract

Semi-purified immobilized esterase, isolated from the seed of Pisum sativum, was found to be an efficient biocatalyst for the facile esterification of pharmaceutically important carboxylic acids. The partially purified esterase precipitated using ammonium sulfate was immobilized using sodium alginate method. After incubation, the immobilized beads so formed were found to be spherical with an average size 3 mm. Immobilized esterase (Km: 580 ug, Vmax: 350 ug/min) to be tested for the esterification of aromatic carboxylic acids was incubated separately with methanol containing benzoic acid, 4-amino-2-chlorobenzoic acid, salicylic acid, and ethanol containing 4-aminobenzoic acid, respectively, at room temperature with constant stirring. Activity of free and immobilized esterase was measured with synthetic substrate, 4-nitrophenyl acetate. The reaction progress was monitored by thin layer chromatography (Hexane: Ethyl acetate) with successive time intervals. The corresponding esters methylbenzoate, 4-amino-2- chloromethylbenzoate, methylsalicylate, and 4-aminoethylbenzoate were analyzed with spectroscopic techniques and determination of physical constants. This confirmed the ability of esterase to transform organic acids smoothly into desired esters. The present studies demonstrated the suitability of preparation and promising procedure for the green synthesis of aromatic esters. Highly purified esterase of P. sativum will afford wide futuristic scope for large scale production of pharmaceutically and/or industrially important products.


Keyword:     Biotransformation Esterification Immobilized esterase Pisum sativum


Citation:

Tak RD, Bhosale AV, Gaikwad DD. Bioesterification of carboxylic acids by immobilized esterase of Pisum sativum. J App Biol Biotech. 2023;11(1):51-54. https://doi.org/10.7324/JABB.2023.110106

Copyright: Author(s). This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.

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