Research Article | Volume 11, Issue 2, March, 2023

Calcium influences biochemical and antioxidant enzymatic activities in tomato fruits during storage

Salma Santhosh Thiyagarajan Chitdeshwari Kavitha Chinnasamy   

Open Access   

Published:  Jan 22, 2023

DOI: 10.7324/JABB.2023.110216

Postharvest quality maintenance of tomatoes is very crucial because of its perishable nature. Calcium, directly involved in the physiological process and enzymatic activities, thereby helps in maintaining the postharvest quality. Increasing calcium concentration reduces respiration and ethylene production in succession; it increases the shelf life and quality of tomatoes. Hence, a field experiment was conducted with different sources and levels of Ca, namely, calcium sulfate, calcium nitrate, calcium silicate, poultry manure, and press mud applied at 0, 20, 40, 60, and 80 kg Ca ha−1. Ripened tomatoes were harvested from each plot. Eight fruits were selected randomly from every treatment and divided into destructive and nondestructive samples, arranged in a factorial completely randomized block design for conducting a storage study. Shelf life, ascorbic acid, lycopene, beta-carotene, and polygalacturonase were analyzed at a 5-day interval up to 15 days. The results revealed that the effect of calcium sources had significant (p ≤ 0.05) impacts on fruit quality. The tomatoes harvested from poultry manure retained the highest shelf life (15 days) at 80 kg Ca ha−1 and the highest ascorbic acid content (37.5 mg 100 g−1) at 60 kg Ca ha−1. However, the lowest lycopene (2.86 mg 100 g−1), beta-carotene (1.81 mg 100 g−1), and lesser polygalacturonase activity (1.99 kj m−2) were obtained with poultry manure at 80 kg Ca ha−1. From the results procured in this experiment, it was inferred that the application of poultry manure at 80 kg Ca ha−1 was found to be effective in improving the quality attributes of hybrid tomato as it imparts multiple essential nutrients along with calcium.

Keyword:     Calcium tomato fruit quality enzyme biochemical compounds


Santhosh S, Chitdeshwari T, Chinnasamy K. Calcium influences biochemical and antioxidant enzymatic activities in tomato fruits during storage. J Appl Biol Biotech. 2023;11(2):158-164.

Copyright: Author(s). This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.

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1. Story EN, Rachel E, Kopec Steven J, Schwartz, Harris GK. An update on the health effects of tomato lycopene. Annu Rev Food Sci Technol 2010;1:189-210.

2. Azad MAK, Islam MS, Hossen MI, Eaton TEJ. Yield and fruit quality of tomato as influenced by calcium and mulching in rooftop cultivation. Agric Sci 2019;10(07):893.

3. Afsana N, Islam MM, Hossain ME, Nizam R, Monalesa N, Hussain MA, et al. Response of tomato (Solanum lycopersicum L.) to salicylic acid and calcium. J Appl Life Sci Int 2017;15:1-7.

4. Dragan Ž, Ban D, Oplani M, Kari L. Influence of post-harvest temperatures on physicochemical quality of tomatoes (Lycopersicon esculentum Mill.). J Food Agric Environ 2010;8(1):21-5.

5. Teka TA. Analysis of the effect of maturity stage on the post-harvest biochemical quality characteristic of tomato fruit. Int Res J Pharm Appl Sci 2013;3(5):180-6.

6. Tola M. Tomato value chain analysis in the central rift valley: the case of DugdaWoreda, East Shoa Zone, Oromia, Ethiopia. MSc Thesis, Haramaya University, Dire Dawa, Ethiopia, 2014.

7. Awas G, Abdisa T, Tolosa K, Chali A. Effect of inter-row spacing with double row arrangement on yield and yield component of tomato (Lycopersicon esculuntum Mill.) at Adami Tulu agricultural research center (Central rift valley of Oromia, Ethiopia). Afr J Agric Res 2010;6(13):2978-81.

8. Zhu Z, Chen Y, Zhang X, Li M. Effect of foliar treatment of sodium selenate on postharvest decay and quality of tomato fruits. Sci Hortic 2016;198:304-10.

9. Kapsiya J, Gungula DT, Tame VT, Bukar N. Effects of storage chemicals and packaging systems on physicochemical characteristics of tomato (Solanum lycopersicum L.) fruits. AASCIT J Biosci 2015;1(3): 41-6.

10. Ali S, Javed HU, Rehman RNU, Sabir IA, Naeem, MS, Siddiqui MZ, et al. Foliar application of some macro and micro nutrients improves tomato growth, flowering and yield. Int J Biosci 2013;3(10):280-7.

11. Pila N, Gol NB, Rao TVR. Effect of post-harvest treatments on physicochemical characteristics and shelf life of tomato (Lycopersicon esculentum Mill.) fruits during storage. Am Euras J Agric Environ Sci 2010;9:470-9.

12. Haleema B, Rab A, Hussain SA, Sajid M, Arif M, Shah ST, et al. Influence of calcium concentrations and sources on the fruit quality of tomato (Lycopersicon esculentum mill) at different storage conditions. Fresenius Environ Bull 2020;29(3):1866-77.

13. Bakshi M, Liné C, Bedolla DE, Stein RJ, Kaegi R, Sarret G, et al. Assessing the impacts of sewage sludge amendment containing nano-TiO2 on tomato plants: a life cycle study. J Hazard Mater 2019;369:191-8.

14. Gao L, Gonda I, Sun H, Ma Q, Bao K, Tieman DM, et al. The tomato pan-genome uncovers new genes and a rare allele regulating fruit flavor. Nat Genet 2019;51(6):1044-51.

15. Hocking B, Tyerman SD, Burton RA, Gilliham M. Fruit calcium: transport and physiology. Front Plant Sci 2016;7:569.

16. Tilahun S, Park DS, Taye AM, Jeong CS. Effects of storage duration on physicochemical and antioxidant properties of tomato (Lycopersicon esculentum Mill.). Korean J Hortic Sci Technol 2017;35(1):88-97.

17. Metkar S, Saptarshi S, Kadam A. Studies on extraction, isolation and applications of lycopene. Indo Am J Pharm Res 2014;3(4):234-42.

18. Moneruzzaman KM, Hossain ABMS, Sani W, Saifuddin M, Alenazi M. Effect of harvesting and storage conditions on the post harvest quality of tomato (Lycopersicon esculentum Mill) cv. Roma VF. Aust J Crop Sci 2009;3(2):113.

19. N'Dri D, Calani L, Mazzeo T, Scazzina F, Rinaldi M, Del Rio D, et al. Effects of different maturity stages on antioxidant content of Ivorian Gnagnan (Solanum indicum L.) berries. Molecules 2010;15(10):7125-38.

20. Nour V, Ionica ME, Trandafir I. Bread enriched in lycopene and other bioactive compounds by addition of dry tomato waste. J Food Sci Technol 2015;52(12):8260-7.

21. Dinis LT, Bernardo S, Conde A, Pimentel D, Ferreira H, Félix L, et al. Kaolin exogenous application boosts antioxidant capacity and phenolic content in berries and leaves of grapevine under summer stress. J Plant Physiol 2016;191:45-53.

22. Kushwaha R, Singh M, Singh V, Puranik V, Kaur D. Variation of bioactive compounds and antioxidant activity during ripening of tomato cultivars. J Food Agric Res 2021;1(1):15-29.

23. Liu L, Shao Z, Zhang M, Wang Q. Regulation of carotenoid metabolism in tomato. Mol Plant 2015;8(1):28-39.

24. Kumar N, Kaur P, Devgan K, Attkan, AK. Shelf life prolongation of cherry tomato using magnesium hydroxide reinforced bionanocomposite and conventional plastic films. J Food Process Preserv 2020;44(4):e14379.

25. Ilahy R, Hdider C, Lenucci MS, Tlili I, Dalessandro G. Antioxidant activity and bioactive compound changes during fruit ripening of high-lycopene tomato cultivars. J Food Compos Anal 2011;24(4- 5):588-95.

26. Williamson DD, Benkeblia N. Effects of gibberellic acid and calcium chloride on colour, phenolic compounds, carotenoids and quality attributes of white cabbage (Brassica oleracea) during storage under refrigeration. Int J Food Sci Nutr Diet 2015;4(7):239-45.

27. Demes R, Satheesh N, Fanta SW. Effect of different concentrations of the gibberellic acid and calcium chloride dipping on quality and shelflife of Kochoro variety tomato. Philipp J Sci 2021;150(1):335-49.

28. Oliveira RQ, Rosa CA, Uetanabaro APT, Azeredo A, Neto AG, Assis SA. Polygalacturonase secreted by yeasts from Brazilian semi-arid environments. Int J Food Sci Nutr 2009;60(7):72-80.

29. Barka EA, Kalantari S, Makhlouf J, Arul J. Impact of UV-C irradiation on the cell wall-degrading enzymes during ripening of tomato (Lycopersicon esculentum L.) fruit. J Agric Food Chem 2000;48(3):667-71.

30. Paniagua C, Ric-Varas P, García-Gago JA, López-Casado G, Blanco-Portales R, Muñoz-Blanco J, et al. Elucidating the role of polygalacturonase genes in strawberry fruit softening. J Exp Bot 2020;71(22):7103-17.

31. Cabanne C, Doneche B. Changes in polygalacturonase activity and calcium content during ripening of grape berries. Am J Ecol Vitic 2001;52(4):331-5.

32. Coutinho PWR, de Moraes Echer M, Braga GC, Guimarães VF, do Carmo Lana M, Alves TN, et al. Effect of pre-harvest calcium silicate on post-harvest quality of tomatoes. Res Soc Dev 2020;9(11):e74791110148.

33. Tejashvini A, Subbarayappa CT, Ramamurthy V, Mukunda GK. Influence of calcium and boron application on quality of tomato. J Pharmacogn Phytochem 2021;10(1):549-52.

34. Shehata SA, Abdelrahman SZ, Megahed M, Abdeldaym EA, El-Mogy MM, Abdelgawad KF. Extending shelf life and maintaining quality of tomato fruit by calcium chloride, hydrogen peroxide, chitosan, and ozonated water. Horticulturae 2021;7(9):309.

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