Research Article | Volume 10, Supplement 2, July, 2022

Process optimization for efficacious biodecolorization of crystal violet by Malaysian Rhodococcus pyridinivorans using monothetic analysis

Maegala Nallapan Maniyam Hazeeq Hazwan Azman Hasdianty Abdullah Nor Suhaila Yaacob   

Open Access   

Published:  Jun 20, 2022

DOI: 10.7324/JABB.2022.10s212

Environmental concern over the discharge of improperly treated textile wastewater has been on the rise. The present study explored the use of the limitedly studied, tropical Rhodococcus pyridinivorans for the decolorization of crystal violet, a typically encountered dye in the textile industry. The effect of agitation speed, temperature, pH, nutritional source, initial dye concentration, and size of inoculum on crystal violet removal were evaluated using one-factor-at-a-time method. Decolorization of 0.6 mM crystal violet carried out at agitated mode showed 73 ± 3% crystal violet removal efficiency in comparison to static mode (43 ± 2%) after 24 h. This crystal violet removal efficiency escalated to 91 ± 2% after optimizing the culture conditions at 45°C and pH 7 using maltose as carbon source and 12% (v/v) size of inoculum. Furthermore, the optimization process reduced the incubation time to achieve almost complete decolorization by 67%. Ultraviolet–visible analysis revealed that the decolorization of crystal violet primarily occurred through biodegradation. The findings from this study validated the potential of R. pyridinivorans as an effective biocatalyst to remediate crystal violet. R. pyridinivorans will be attempted to decolorize different triphenylmethane dyes, namely, malachite green in future studies.

Keyword:     Crystal violet Decolorization Optimization one-factor-at-a-time Rhodococcus pyridinivorans


Maniyam MN, Azman HH, Abdullah H, Yaacob NS. Process optimization for efficacious biodecolorization of crystal violet by Malaysian Rhodococcus pyridinivorans using monothetic analysis. J App Biol Biotech. 2022;10(Suppl 2):107-113. DOI: 10.7324/JABB.2022.10s212

Copyright: Author(s). This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.

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