Research Article | Volume 10, Supplement 2, July, 2022

Process optimization for efficacious biodecolorization of crystal violet by Malaysian Rhodococcus pyridinivorans using monothetic analysis

Maegala Nallapan Maniyam Hazeeq Hazwan Azman Hasdianty Abdullah Nor Suhaila Yaacob   

Open Access   

Published:  Jun 20, 2022

DOI: 10.7324/JABB.2022.10s212
Abstract

Environmental concern over the discharge of improperly treated textile wastewater has been on the rise. The present study explored the use of the limitedly studied, tropical Rhodococcus pyridinivorans for the decolorization of crystal violet, a typically encountered dye in the textile industry. The effect of agitation speed, temperature, pH, nutritional source, initial dye concentration, and size of inoculum on crystal violet removal were evaluated using one-factor-at-a-time method. Decolorization of 0.6 mM crystal violet carried out at agitated mode showed 73 ± 3% crystal violet removal efficiency in comparison to static mode (43 ± 2%) after 24 h. This crystal violet removal efficiency escalated to 91 ± 2% after optimizing the culture conditions at 45°C and pH 7 using maltose as carbon source and 12% (v/v) size of inoculum. Furthermore, the optimization process reduced the incubation time to achieve almost complete decolorization by 67%. Ultraviolet–visible analysis revealed that the decolorization of crystal violet primarily occurred through biodegradation. The findings from this study validated the potential of R. pyridinivorans as an effective biocatalyst to remediate crystal violet. R. pyridinivorans will be attempted to decolorize different triphenylmethane dyes, namely, malachite green in future studies.


Keyword:     Crystal violet Decolorization Optimization one-factor-at-a-time Rhodococcus pyridinivorans


Citation:

Maniyam MN, Azman HH, Abdullah H, Yaacob NS. Process optimization for efficacious biodecolorization of crystal violet by Malaysian Rhodococcus pyridinivorans using monothetic analysis. J App Biol Biotech. 2022;10(Suppl 2):107-113. DOI: 10.7324/JABB.2022.10s212

Copyright: Author(s). This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.

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Reference

1. Al-Garni SM, Ghanem KM, Kabli SA, Biag AK. Decolorization of crystal violet by mono and mixed bacterial culture techniques using optimized culture conditions. Pol J Environ Stud 2013;22:1297-306.

2. Ali HM, Shehata SF, Ramadan KM. Microbial decolorization and degradation of crystal violet dye by Aspergillus niger. Int J Environ Sci Technol 2016;13:2917-26. https://doi.org/10.1007/s13762-016-1117-x

3. Morales-Álvarez ED, Rivera-Hoyos CM, Poveda-Cuevas SA, Reyes-Guzmán EA, Pedroza-Rodríguez AM, Reyes-Montaño EA, et al. Malachite green and crystal violet decolorization by Ganoderma lucidum and Pleurotus ostreatus supernatant and by rGlLCC1 and rPOXA 1B concentrates: Molecular docking analysis. Appl Biochem Biotechnol 2018;184:794-805. https://doi.org/10.1007/s12010-017-2560-y

4. Rojas-Valencia OG, Estrada-Flores M, Reza-San-Germán CM, Ledezma-Martínez JL, Hernández-Fuentes J. High removal of toxic crystal violet dye using a thermally treated activated carbon fiber felt. Rev Mex Ing Quim 2021;20:Mat2444. https://doi.org/10.24275/rmiq/Mat2444

5. Gul A, Ullah R, Sun J, Munir T, Bai S. Synthesis of mesoporous TiO2/BMMs via hydrothermal method and its potential application toward adsorption and photocatalytic degradation of crystal violet from aqueous solution. Arab J Chem 2022;15:103530. https://doi.org/10.1016/j.arabjc.2021.103530

6. Li G, Peng L, Ding Z, Liu Y, Gu Z, Zhang L, et al. Decolorization and biodegradation of triphenylmethane dyes by a novel Rhodococcus qingshengii JB301 isolated from sawdust. Ann Microbiol 2014;64:1575-86. https://doi.org/10.1007/s13213-014-0801-7

7. Talha MA, Goswami M, Giri BS, Sharma A, Rai BN, Singh RS. Bioremediation of congo red dye in immobilized batch and continuous packed bed bioreactor by Brevibacillus parabrevis using coconut shell bio-char. Bioresour Technol 2018;252:37-43. https://doi.org/10.1016/j.biortech.2017.12.081

8. Mohanty SS, Kumar A. Enhanced degradation of anthraquinone dyes by microbial monoculture and developed consortium through the production of specific enzymes. Sci Rep 2021;11:1-15. https://doi.org/10.1038/s41598-021-87227-6

9. Sjahrir F, Maniyam MN, Ibrahim AL, Cass AE. Biotransformation of acrylonitrile using immobilized cells of Rhodococcus UKMP-5M as biocatalyst. Indian J Fund Appl Life Sci 2016;6:58-67.

10. Tengku-Mazuki TA, Subramaniam K, Zakaria NN, Convey P, Khalil KA, Lee GL, et al. Optimization of phenol degradation by Antarctic bacterium Rhodococcus sp. Antarct Sci 2020;32:486-95. https://doi.org/10.1017/S0954102020000358

11. Ibrahim S, Khalil KA, Zahri KN, Gomez-Fuentes C, Convey P, Zulkharnain A, et al. Biosurfactant production and growth kinetics studies of the waste canola oil-degrading bacterium Rhodococcus erythropolis AQ5-07 from Antarctica. Molecules 2020;25:3878. https://doi.org/10.3390/molecules25173878

12. Suhaila YN, Hasdianty A, Maegala NM, Aqlima A, Hazwan AH, Rosfarizan M, et al. Biotransformation using resting cells of Rhodococcus UKMP-5M for phenol degradation. Biocatal Agric Biotechnol 2019;21:101309. https://doi.org/10.1016/j.bcab.2019.101309

13. Zhuang M, Sanganyado E, Zhang X, Xu L, Zhu J, Liu W, et al. Azo dye degrading bacteria tolerant to extreme conditions inhabit nearshore ecosystems: Optimization and degradation pathways. J Environ Manag 2020;261:110222. https://doi.org/10.1016/j.jenvman.2020.110222

14. Singh V, Haque S, Niwas R, Srivastava A, Pasupuleti M, Tripathi C. Strategies for fermentation medium optimization: An in-depth review. Front Microbiol 2017;7:2087. https://doi.org/10.3389/fmicb.2016.02087

15. Hasdianty A, Suhaila YN, Hazwan AH, Maniyam MN, Fadzli AM, Ibrahim AL. Inferring the evolutionary relationship of 23 Malaysian Rhodococcus isolates with potential as cholesterol degrading bacteria. Biocatal Agric Biotechnol 2020;30:101840. https://doi.org/10.1016/j.bcab.2020.101840

16. Bharagava RN, Mani S, Mulla SI, Saratale GD. Degradation and decolourization potential of an ligninolytic enzyme producing Aeromonas hydrophila for crystal violet dye and its phytotoxicity evaluation. Ecotoxicol Environ Saf 2018;156:166-75. https://doi.org/10.1016/j.ecoenv.2018.03.012

17. Gao T, Qin D, Zuo S, Peng Y, Xu J, Yu B, et al. Decolorization and detoxification of triphenylmethane dyes by isolated endophytic fungus, Bjerkandera adusta SWUSI4 under non-nutritive conditions. Bioresour Bioprocess 2020;7:1-12. https://doi.org/10.1186/s40643-020-00340-8

18. Ngo AC, Conrad C, Baraibar ÁG, Matura A, Van Pee KH, Tischler D. Characterization of two hydrogen peroxide resistant peroxidases from Rhodococcus opacus 1CP. Appl Sci 2021;11:7941. https://doi.org/10.3390/app11177941

19. Vignali E, Tonin F, Pollegioni L, Rosini E. Characterization and use of a bacterial lignin peroxidase with an improved manganese-oxidative activity. Appl Microbiol Biotechnol 2018;102:10579-88. https://doi.org/10.1007/s00253-018-9409-3

20. Singh R, Grigg JC, Qin W, Kadla JF, Murphy ME, Eltis LD. Improved manganese-oxidizing activity of DypB, a peroxidase from a lignolytic bacterium. ACS Chem Biol 2013;8:700-6. https://doi.org/10.1021/cb300608x

21. Chen CC, Liao HJ, Cheng CY, Yen CY, Chung YC. Biodegradation of crystal violet by Pseudomonas putida. Biotechnol Lett 2007;29:391-6. https://doi.org/10.1007/s10529-006-9265-6

22. Ayed L, Cheriaa J, Laadhari N, Cheref A, Bakhrouf A. Biodegradation of crystal violet by an isolated Bacillus sp. Ann Microbiol 2009;59:267-72. https://doi.org/10.1007/BF03178327

23. Maniyam MN, Hari M, Yaacob NS. Enhanced methylene blue decolourization by Rhodococcus strain UCC 0003 grown in banana peel agricultural waste through response surface methodology. Biocatal Agric Biotechnol 2020;23:101486. https://doi.org/10.1016/j.bcab.2019.101486

24. Adenan NH, Lim YY, Ting, AS. Nocardiopsis sp. for the removal of triphenylmethane dyes: Decolorization and optimization studies. Water Air Soil Pollut 2021;232:1-17. https://doi.org/10.1007/s11270-021-05377-9

25. Cao DJ, Wang JJ, Zhang Q, Wen YZ, Dong B, Liu RJ, et al. Biodegradation of triphenylmethane dye crystal violet by Cedecea davisae. Spectrochim Acta A Mol Biomol Spectrosc 2019;210:9-13. https://doi.org/10.1016/j.saa.2018.11.004

26. Wi?niewska KM, Twarda-Clapa A, Bia?kowska AM. Screening of novel laccase producers-isolation and characterization of cold-adapted laccase from Kabatiella bupleuri G3 capable of synthetic dye decolorization. Biomolecules 2021;11:828. https://doi.org/10.3390/biom11060828

27. Gan L, Cheng Y, Palanisami T, Chen Z, Megharaj M, Naidu R. Pathways of reductive degradation of crystal violet in wastewater using free-strain Burkholderia vietnamiensis C09V. Environ Sci Pollut Res 2014;21:10339-48. https://doi.org/10.1007/s11356-014-3037-y

28. Saratale RG, Gandhi SS, Purankar MV, Kurade MB, Govindwar SP, Oh SE, et al. Decolorization and detoxification of sulfonated azo dye CI Remazol red and textile effluent by isolated Lysinibacillus sp. RGS. J Biosci Bioeng 2013;115:658-67. https://doi.org/10.1016/j.jbiosc.2012.12.009

29. Shanmugam S, Ulaganathan P, Sivasubramanian S, Esakkimuthu S, Krishnaswamy S, Subramaniam S. Trichoderma asperellum laccase mediated crystal violet degradation-Optimization of experimental conditions and characterization. J Environ Chem Eng 2017;5:222-31. https://doi.org/10.1016/j.jece.2016.11.044

30. Jakhrani BA, Phulpoto IA, Phulpoto AH, Babar MM, Panhyar AA, Channa N, et al. Improved decolorization of triphenylmethane dyes by halo-thermotolerant bacteria isolated from hypersaline environments. Int J Environ Sci Technol 2021;18:1-14. https://doi.org/10.1007/s13762-021-03320-3

31. Maniyam MN, Sjahrir F, Ibrahim AL, Cass AE. Biodegradation of cyanide by acetonitrile-induced cells of Rhodococcus sp. UKMP-5M. J Gen Appl Microbiol 2013;59:393-404. https://doi.org/10.2323/jgam.59.393

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