Research Article | Volume 10, Supplement 2, July, 2022

Ex-situ biofilm mediated approach for bioremediation of selected heavy metals in wastewater of textile industry

Anu Kumar Shivani Bhanu Krishan Mrinal Samtiya Tejpal Dhewa   

Open Access   

Published:  Jun 20, 2022

DOI: 10.7324/JABB.2022.10s209
Abstract

Industrialization plays a major role in strengthening the economy of any country. However, these industries directly or indirectly affect the environment. Industrial wastewater discharge has been reported with certain heavy metals such as chromium, lead, cobalt, and others which are a potential hazard to the water bodies and humans as well. Biofilm is an applied method in the fields of bioremediation for reliving this emerging problem and in the efficient removal of heavy metals from wastewater. Biofilms of Escherichia coli and petroleum soil isolated microorganisms (PSIM) were developed at the V bottom of 96 well microtiter plate. The contaminated water sample was collected from the textile industry, Solan, Himachal Pradesh, India. The biofilms were incubated with the industrial water tested for heavy metals assuming the microbes have the potential to assimilate the heavy metals up to 5 mg/mL of concentration. After the incubation for 1–2 weeks, the microorganisms were able to reduce the level of heavy metals present in the samples which was conveyed by biomass comparison of microorganisms in the successive intervals of time. 0.74 × 1010 cells/mL and 0.77 × 1010cell/mL of E. coli and PSIM biofilms were able to tolerate the metal toxicity on incubation for 2 weeks at the highest concentration due to the functional group present extracellular polymeric substance which forms complexes with heavy metals. This leads to the fact that these biofilms have assimilated the heavy metals and are potent for the removal of heavy metals from industrial wastewater.


Keyword:     Biofilms Bioremediation Heavy metals Microtiter plate Textiles Wastewater


Citation:

Kumar A, Shivani, Krishan B, Samtiya M, Dhewa T. Ex-situ biofilm mediated approach for bioremediation of selected heavy metals in wastewater
of textile industry. J App Biol Biotech. 2022;10(Suppl 2):85-90. DOI: https://doi.org/10.7324/JABB.2022.10s209

Copyright: Author(s). This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.

HTML Full Text
Reference

1. Tchounwou PB, Yedjou CG, Patlolla AK, Sutton DJ. Heavy metal toxicity and the environment. Exp Suppl 2012;101:133-64. Luch A, editor. Molecular, Clinical and Environmental Toxicology: Volume 3: Environmental Toxicology. Basel: Springer; 2012. https://doi.org/10.1007/978-3-7643-8340-4_6

2. Farkhondeh T, Naseri K, Esform A, Aramjoo H, Naghizadeh A. Drinking water heavy metal toxicity and chronic kidney diseases: A systematic review. Rev Environ Health 2021;36:359-66. https://doi.org/10.1515/reveh-2020-0110

3. Vennam S, Georgoulas S, Khawaja A, Chua S, Strouthidis NG, Foster PJ. Heavy metal toxicity and the aetiology of glaucoma. Eye (Lond) 2020;34:12937. https://doi.org/10.1038/s41433-019-0672-z

4. Malik A. Metal bioremediation through growing cells. Environ Int 2004;30:261-78. https://doi.org/10.1016/j.envint.2003.08.001

5. Martin SG. Human health effects of heavy metals. Environ Sci Technol Brief Citizens 2009;15:1-6.

6. Goyer RA. Lead toxicity: From overt to subclinical to subtle health effects. Environ Health Perspect 1990;86:177-81. https://doi.org/10.1289/ehp.9086177

7. Goyer RA. Lead toxicity: current concerns. Environ Health Perspect 1993;100:177-87. https://doi.org/10.1289/ehp.93100177

8. Das KK, Das SN, Dhundasi SA. Nickel, its adverse health effects and oxidative stress. Indian J Med Res 2008;128:412-25.

9. Gomes LC, Moreira JM, Simões M, Melo LF, Mergulhão FJ. Biofilm localization in the vertical wall of shaking 96-well plates. Scientifica (Cairo) 2014;2014:2310831. https://doi.org/10.1155/2014/231083

10. Muhammad MH, Idris AL, Fan X, Guo Y, Yu Y, Jin X, et al. Beyond risk: Bacterial biofilms and their regulating approaches. Front Microbiol 2020;11:928. https://doi.org/10.3389/fmicb.2020.00928

11. Khatoon Z, McTiernan CD, Suuronen EJ, Mah TF, Alarcon EI. Bacterial biofilm formation on implantable devices and approaches to its treatment and prevention. Heliyon 2018;4:e01067. https://doi.org/10.1016/j.heliyon.2018.e01067

12. Coughlan LM, Cotter PD, Hill C, Alvarez-Ordóñez A. New weapons to fight old Enemies: Novel strategies for the (bio) control of bacterial biofilms in the food industry. Front Microbiol 2016;7:1641. https://doi.org/10.3389/fmicb.2016.01641

13. Maksimova YG. Microbial biofilms in biotechnological processes. Appli Biochem Microbiol 2014;50:750-60. https://doi.org/10.1134/S0003683814080043

14. Singh R, Paul D, Jain RK. Biofilms: Implications in bioremediation. Trends Microbiol 2006;14:389-97. https://doi.org/10.1016/j.tim.2006.07.001

15. Yadav S, Chandra R. Biofilm-mediated bioremediation of pollutants from the environment for sustainable development. In: Yadav MK, Singh B, editors. New and Future Developments in Microbial Biotechnology and Bioengineering: Microbial Biofilms. Ch. 14. Amsterdam, Netherlands: Elsevier; 2020. p. 177-203. https://doi.org/10.1016/B978-0-444-64279-0.00014-1

16. Mohapatra RK, Behera SS, Patra JK, Thatoi H, Parhi PK. Potential application of bacterial biofilm for bioremediation of toxic heavy metals and dye-contaminated environments. In: New and Future Developments in Microbial Biotechnology and Bioengineering: Microbial Biofilms. Amsterdam, Netherlands: Elsevier; 2020. p. 267-81. https://doi.org/10.1016/B978-0-444-64279-0.00017-7

17. Shukla K, Mangwani S, Karley N, Rao TS. Bacterial biofilms and genetic regulation for metal detoxification. In: Das S, Dash HR, editors. Handbook of Metal-Microbe Interactions and Bioremediation. 1st ed. Boca Raton: Taylor and Francis, CRC Press; 2017. p. 16. https://doi.org/10.1201/9781315153353-22

18. Wang L, Chen W, Song X, Li Y, Zhang W, Zhang H, et al. Cultivation substrata differentiate the properties of river biofilm EPS and their binding of heavy metals: A spectroscopic insight. Environ Res 2020;182:109052. https://doi.org/10.1016/j.envres.2019.109052

19. Dar SA, Lone FA, Dar SA, Bhat RA, Bashir I, Mir SA, et al. Biofilm: An innovative modern technology for aquatic pollution remediation. In: Bhat RA, Hakeem KR, Dervash MA, editors. Bioremediation and Biotechnology, Vol 2: Degradation of Pesticides and Heavy Metals. Cham: Springer International Publishing; 2020. p. 207-19. https://doi.org/10.1007/978-3-030-40333-1_12

20. Lovley DR, Coates JD. Bioremediation of metal contamination. Curr Opin Biotechnol 1997;8:285-9. https://doi.org/10.1016/S0958-1669(97)80005-5

21. Sagar SS, Chavan RP, Patil CL, Shinde DN, Kekane SS. Physico-chemical parameters for testing of water, a review paper. Int J Chem Stu 2015;3:24-8.

22. Qualitative Tests on 3d-metal Ions (Cr, Mn, Fe, Co, Ni, Cu). Available from: http://staff.buffalostate.edu/nazareay/che112/ex9.html [Last accessed on 2021 Aug 25].

23. Thyssen JP, Skare L, Lundgren L, Menné T, Johansen JD, Maibach HI, et al. Sensitivity and specificity of the nickel spot (dimethylglyoxime) test. Contact Dermatitis 2010;62:279-88. https://doi.org/10.1111/j.1600-0536.2010.01709.x

24. Swann MH, Adams ML. Rapid colorimetric method for nitrates. Anal Chem 1956;28:1630. https://doi.org/10.1021/ac60118a038

25. Merritt JH, Kadouri DE, O'Toole GA. Growing and analyzing static biofilms. Curr Protoc Microbiol 2011;22:1-18. https://doi.org/10.1002/9780471729259.mc01b01s22

26. Coffey BM, Anderson GG. Biofilm formation in the 96-well microtiter plate. Methods Mol Biol.2014;1149:631-41. https://doi.org/10.1007/978-1-4939-0473-0_48

27. Müsken M, Di Fiore S, Römling U, Häussler S. A 96-well-plate-based optical method for the quantitative and qualitative evaluation of pseudomonas aeruginosa biofilm formation and its application to susceptibility testing. Nat Protoc 2010;5:1460-9. https://doi.org/10.1038/nprot.2010.110

28. Bybin VA, Belogolova GA, Markova YA, Sokolova MG, Sidorov AV, Gordeeva ON, et al. Influence of heavy metals and arsenic on survival and biofilm formation of some saprotrophic soil microorganisms. Water Air Soil Pollut 2021;232:343. https://doi.org/10.1007/s11270-021-05288-9

29. Jardine JL. Potential bioremediation of heavy metal ions, polycyclic aromatic hydrocarbons and biofilms with South African hot spring bacteria. Bioremed J 2022;26:1-9. https://doi.org/10.1080/10889868.2021.1964429

30. Maurya A, Kumar PS, Raj A. Characterization of biofilm formation and reduction of hexavalent chromium by bacteria isolated from tannery sludge. Chemosphere 2022;286:131795. https://doi.org/10.1016/j.chemosphere.2021.131795

31. Alfadaly RA, Elsayed A, Hassan RY, Noureldeen A, Darwish H, Gebreil AS. Microbial sensing and removal of heavy metals: Bioelectrochemical detection and removal of chromium (vi) and cadmium (ii). Molecules 2021;26:2549. https://doi.org/10.3390/molecules26092549

Article Metrics
37 Views 41 Downloads 78 Total

Year

Month

Related Search

By author names

Similar Articles

Practiced Gram negative bacteria from dyeing industry effluents snub metal toxicity to survive

Channarayapatna-Ramesh Sunilkumar , Lobo Rachel, Gurulingaiah Bhavya, Kujur Swati , R. Samaga Sridhar, J. Samanth Kumar, K. Ramachandra Kini, H. S. Prakash, Nagaraja Geetha

Bioremediation of heavy metals from aquatic environment through microbial processes: A potential role for probiotics?

Marie Andrea Laetitia Huët, Daneshwar Puchooa

Microbial synthesis of magnetite nanoparticles for arsenic removal

Gopal Samy Balakrishnan, Karthik Rajendran, Jegatheesan Kalirajan

Characterization of Calotropis procera root peroxidase and its potential to mediate remediation of phenolic pollutant from petroleum refinery effluent

Enoch Banbilbwa Joel, Simon Gabriel Mafulul, Ezra Adams Jeremiah, Adepeju Aberuagba, Raphael Idowu Adeoye, Lazarus Joseph Goje, Adedoyin Igunnu, Sylvia Omonirume Malomo

Bioremediation of hazardous azo dye methyl red by a newly isolated Enterobacter asburiae strain JCM6051 from industrial effluent of Uttarakhand regions

Swati, Padma Singh

Microplastics accumulation in agricultural soil: Evidence for the presence, potential effects, extraction, and current bioremediation approaches

Varsha Yadav, Saveena Dhanger, Jaigopal Sharma

Seasonal effect on the diversity of soil fungi and screening for arsenic tolerance and their remediation

Dheeraj Pandey, Harbans Kaur Kehri, Ifra Zoomi, Shweta Chaturvedi, Kanhaiya Lal Chaudhary

Nanotechnology for the bioremediation of heavy metals and metalloids

Urja Sharma, Jai Gopal Sharma

Bioremediation and Waste Management for Environmental Sustainability

Ajar Nath Yadav, Deep Chandra Suyal, Divjot Kour, Vishnu D. Rajput, Ali Asghar Rastegari, Joginder Singh

Microbe-mediated bioremediation: Current research and future challenges

Divjot Kour, Sofia Shareif Khan, Harpreet Kour, Tanvir Kaur, Rubee Devi, Pankaj Kumar Rai, Christina Judy, Chloe McQuestion, Ava Bianchi, Sara Spells, Rajinikanth Mohan, Ashutosh Kumar Rai, Ajar Nath Yadav

Bioremediation— sustainable tool for diverse contaminants management: Current scenario and future aspects

Manali Singh, Kuldeep Jayant, Shivani Bhutani, Anshi Mehra, Tanvir Kaur, Divjot Kour, Deep Chandra Suyal, Sangram Singh, Ashutosh Kumar Rai, Ajar Nath Yadav

Industrial biotechnology: An Indian perspective

Kumud Tiwari, Garima Singh, Gajender Singh, Sonika Kumari Sharma, Samarendra Kumar Singh

Nanotechnology for the bioremediation of organic and inorganic compounds in aquatic ecosystem/marine ecosystem

Ishta Kaul, Jai Gopal Sharma

Microbes as a potential bioremediation tool for atrazine-contaminated soil: A review

Chiranjib Mili, Sanjib Kalita, Subham Roy

Microbe-mediated remediation of dyes: Current status and future challenges

Kriti Akansha, Tanvir Kaur, Ashok Yadav, Divjot Kour, Ashutosh Kumar Rai, Sangram Singh, Shashank Mishra, Lalit Kumar, Kanika Miglani, Karan Singh, Ajar Nath Yadav

Sustainable biodegradation of textile dye reactive blue 222 by the novel strain Enterobacter CU2004, isolated from the industrial waste: A design of experiment based optimization study and characterisation of metabolites

Vasantha Veerappa Lakshmaiah, Anish Nag, Suresh Gotekar, Sunil More, Shobha K. Jayanna

Beneficial fungal communities for sustainable development: Present scenario and future challenges

Divjot Kour, Sofia Sharief Khan, Seema Ramniwas, Sanjeev Kumar, Ashutosh Kumar Rai, Sarvesh Rustagi, Kundan Kumar Chaubey, Sangram Singh, Ajar Nath Yadav,, Amrik Singh Ahluwalia

Bacillus species for sustainable management of heavy metals in soil: Current research and future challenges

Diyashree Karmakar, Shanu Magotra, Rajeshwari Negi, Sanjeev Kumar, Sarvesh Rustagi, Sangram Singh, Ashutosh Kumar Rai, Divjot Kour, Ajar Nath Yadav,

Evaluation of heavy metals in selected fruits in Umuahia market, Nigeria: Associating toxicity to effect for improved metal risk assessment

Uroko Robert Ikechukwu, Victor Eshu Okpashi, Uchenna Nancy Oluomachi, Nwuke Chunedu Paulinus, Nduka Florence Obiageli, Ogbonnaya Precious

Effect of different industrial and domestic effluents on growth, yield, and heavy metal accumulation in Turnip (Brassica rapa L.)

Noor ul Ain, Qurat ul Ain, Sadaf Javeria, Sana Ashiq, Kanwal Ashiq, Muhammad Sufyan Akhtar