Research Article | Volume 10, Supplement 1, March, 2022

Identification and characterization of acidosis on in vitro rumen fermentation with feeds based on grass, rice bran, concentrate, and tofu pulp

Darwin Tiya Humaira Ami Muliawati   

Open Access   

Published:  Apr 15, 2022

DOI: 10.7324/JABB.2022.10s108

The present study evaluated and characterized various types of biomass fermented in the rumen culture. A series of batch experiments were conducted to assess any potential organic acid build-up in the rumen culture fermenting different substrates of biomass. Results showed that pH depression occurred within 2 h of incubation in the rumen culture fermenting rice bran and/or concentrate in which the pH dropped from the neutral level (pH 6.9 ± 0.15) to the acidic level (pH 5.6 ± 0.15). However, at the same period of incubation, the fermentation of grass and tofu pulp in the rumen culture did not have a dramatic drop of pH in which the pH was somewhat stable between 7.0 and 6.85. Organic acids, such as acetic acid and lactic acid, were accumulated in the digesters of rumen culture fermenting rice bran and/or concentrate, suggesting that the culture was acidified which led to ruminal acidosis.

Keyword:     Rumen Acidosis Fermentation Biomass


Darwin, Humaira T, Muliawati A. Identification and characterization of acidosis on in vitro rumen fermentation with feeds based on grass, rice bran, concentrate, and tofu pulp. J App Biol Biotech. 2022;10(2):53-58 DOI: 10.7324/JABB.2022.10s108

Copyright: Author(s). This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.

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