Characterization and comparative assessment of bactericidal activity of carbon nanodots (CDs) and nanoparticles (CNPs) prepared from soot's of clarified butter and mustard oil, respectively

Vikas Pahal Pankaj Kumar Rahul Kumar Parveen Kumar Vinod Kumar   

Open Access   

Published:  Jun 21, 2022


Carbon nanoparticles (CNPs) are carbon-based nanomaterial with dimensions in the range of 1–100 nm. In the present research, an ecofriendly, simple, and highly reproducible method was used to prepare the CNPs from the soot of clarified butter (carbon dots) and mustard oil (carbon nanospheres) in both pristine and oxidized forms. The obtained CNPs were subjected to various analyses such as UV-visible, Fourier transform infrared (FTIR), dynamic light scattering, high-resolution transmission electron microscopy, energy-dispersive X-ray, and X-ray diffraction (XRD). The analyses demonstrate that the size of butter-originated CNPs was found in the ranges of 10–90 nm (raw) and 5–20 nm (oxidized), whereas, in the case of mustard oil-originated CNPs, the size was observed in the ranges of 100–150 nm (raw) and 50–80 nm (oxidized). As per zeta potential results, the net surface charges on CNPs were observed as −9.05 and −14.6 mV in the case of raw and oxidized CNPs from butter, respectively, and −12.7 and −20.1 mV in the case of raw and oxidized CNPs from mustard oil, respectively. XRD results showed the typical graphitic crystalline nature of both kinds of CNPs irrespective of their initial raw material. FTIR results confirmed hydroxyl, carboxyl, carbonyl, and amide groups on CNPs that help in their capping and stabilization in the solvent media. Five bacterial strains, Staphylococcus aureus, Escherichia coli, Staphylococcus epidermidis, Klebsiella pneumoniae, and Moraxella catarrhalis, were used to assess the bactericidal potential of synthesized CNPs using agar-well and 2,3-bis-(2-methoxy-4-nitro-5-sulfophenyl)-2Htetrazolium-5-carboxanilide-colorimetric methods. Butter-mediated oxidized CNPs were the most effective bactericidal agent against all the bacterial strains compared to mustard-originated CNPs. Furthermore, CNPsmediated toxicity towards bacteria was both size and concentration dependent. Staphylococcus aureus and S. epidermidis were the most sensitive [minimum inhibitory concentration (MIC): 800 µg/ml] and resistant (MIC: 2.0 mg/ml) bacteria, respectively, towards CNPs-mediated toxicity. The synthesized CNPs were devoid of any metallic impurities and hence worthy of being used in various applications like imaging, labeling, sensortechnology, and environment monitoring and as an antibacterial agent.

Keyword:     Carbon nanodots carbon nanospheres bactericidal effect XTT-colorimetric assay


Pahal V, Kumar P, Kumar R, Kumar P, Kumar V. Characterization and comparative assessment of bactericidal activity of carbon nanodots (CDs) and nanoparticles (CNPs) prepared from soot's of clarified butter and mustard oil, respectively. J Appl Biol Biotech, 2022. Online First.

Copyright: Author(s). This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.

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1.Singh S, Singh D, Singh SP, Pandey AK. Candle soot derived carbon nanoparticles: assessment of physico-chemical properties, cytotoxicity and genotoxicity. Chemosphere 2019;214:130-5.

2. Ashfaq M, Verma N, Khan S. Highly effective Cu/Zn-carbon micro/ nanofiber-polymer nanocomposite-based wound dressing biomaterial against the Pseudomonas aeruginosa multi- and extensively drugresistant strains. Mater Sci Eng C 2017;77:630-41.

3. Tripathi KM, Sachan A, Castro M, Choudhary V, Sonkar SK, Feller JF. Green carbon nanostructured quantum resistive sensors to detect volatile biomarkers. Sustain Mater Technol 2018;16:1-11.

4. Coleman BR, Knight T, Gies V, Jakubek ZJ, Zou S. Manipulation and quantification of graphene oxide flake size: photoluminescence and cytotoxicity. ACS Appl Mater Interfaces 2017;9:28911-921.

5. Khare P, Singh A, Verma S, Bhati A, Sonker AK, Tripathi KM, et al. Sunlight-induced selective photocatalytic degradation of methylene blue in bacterial culture by pollutant soot derived nontoxic graphene nanosheets. ACS Sustain Chem Eng 2018;6:579-89.

6. Pankaj A, Tewari K, Singh S, Singh SP. Waste candle soot derived nitrogen doped carbon dots based fluorescent sensor probe: an efficient and inexpensive route to determine Hg(II) and Fe(III) from water. J Environ Chem Eng 2018;6:5561-9.

7. Sharma A, Das J. Small molecules derived carbon dots: synthesis and applications in sensing, catalysis, imaging, and biomedicine. J Nanobiotech 2019;17(1):92.

8. Zulfajri M, Abdelhamid HN, Sudewi S, Dayalan S, Rasool A, Habib A, et al. Plant part-derived carbon dots for biosensing. Biosensors (Basel) 2020;10(6):68.

9. Dong X, Bond AE, Pan N, Coleman M, Tang Y, Sun YP, et al. Synergistic photoactivated antimicrobial effects of carbon dots combined with dye photosensitizers. Int J Nanomedicine 2018;13:8025-35.

10. Jijie R, Barras A, Bouckaert J, Dumitrascu N, Szunerits S, Boukherroub R. Enhanced antibacterial activity of carbon dots functionalized with ampicillin combined with visible light triggered photodynamic effects. Colloids Surf B Biointerfaces 2018;170:347-54.

11. Tejwan N, Saha SK, Das J. Multifaceted applications of green carbon dots synthesized from renewable sources. Adv Colloid Interface Sci 2020;275:102046.

12. Lin X, Su J, Lin H, Sun X, Liu B, Kankala RK, et al. Luminescent carbon nanodots based aptasensors for rapid detection of kanamycin residue. Talanta 2019;202:452-59.

13. Lu S, Wu D, Li G, Lv Z, Chen L, Chen Z, et al. Carbon dots-based ratio metric nanosensor for highly sensitive and selective detection of mercury(II) ions and glutathione. RSC Adv 2016;6(105):103169-77.

14. Wang Y, Zhu Y, Yu S, Jiang C. Fluorescent carbon dots: rational synthesis, tunable optical properties, and analytical applications. RSC Adv 2017;7(65):40973-89.

15. Roshni V, Misra S, Santra S, Divya O. One pot green synthesis of C-dots from groundnuts and its application as Cr(VI) sensor and in vitro bioimaging agent. J Photochem 2018;375:28-36.

16. Wang L, Yuan Z, Karahan HE, Wang Y, Sui X, Liu F, Chen Y. Nanocarbon materials in water disinfection: state-of-the-art and future directions. Nanoscale 2019;11(20):9819-39.

17. Yan F, Jiang Y, Sun X, Bai Z, Zhang Y, Zhou X. Surface modification and chemical functionalization of carbon dots: a review. Mikrochim Acta 2018;185(9):424.

18. Mohammed MKA, Duha SA, Mohammad RM. Studying antimicrobial activity of carbon nanotubes decorated with metal-doped ZnO hybrid materials. Mater Res Express 2019;6:055404.

19. Rahman G, Najaf Z, Mehmood A, Bilal S, Shah AHA, Mian SA, et al. An overview of the recent progress in the synthesis and applications of carbon nanotubes. C J Carbon Res 2019;5:3.

20. Sun M, Qu A, Hao C, Wu X, Xu L, Xu C, et al. Chiral up conversion heterodimers for quantitative analysis and bioimaging of antibioticresistant bacteria in vivo. Adv Mater 2018;30(50):e1804241.

21. Cui F, Ye Y, Ping J, Sun X. Carbon dots: current advances in pathogenic bacteria monitoring and prospect applications. Biosens Bioelectron 2020;156:112085.

22. Jones K, Patel N, Levy M, Storeygard A, Balk D, Gittleman JL, et al. Global trends in emerging infectious diseases. Nature 2008;451:990- 3.

23. Zhou J, Zhou H, Tang J, Deng S, Yan F, Li W, et al. Carbon dots doped with heteroatoms for fluorescent bioimaging: a review. Microchim Acta 2016;184:343-68.

24. Lakshmi SD, Pramod KA, Gurumurthy H. Activated carbon nanoparticles from biowaste as new generation antimicrobial agents: a review. Nano-Struct Nano-Objects 2018;16:306-21.

25. Kumar R, Kumar VB, Gedanken A. Sonochemical synthesis of carbon dots, mechanism, effect of parameters, and catalytic, energy, biomedical and tissue engineering applications. Ultrason Sonochem 2020;64:105009.

26. Khare C. Brassica campestris Linn. var. rapa (L.) Hartm. In: Khare C (ed.). Indian medicinal plants, Springer, New York, NY, 2007.

27. Agrawal MK, Rathore D, Goyal S, Varma A, Varma A. Antibacterial efficacy of Brassica campestris root, stem and leaves extracts. Int J Adv Res 2013;5:131-5.

28. Kaushik R, Jain J, Rai P. Therapeutic potentials of cow derived products- a review. Int J Pharm Sci Res 2016;7(4):1383-90.

29. Khameneh B, Iranshahy M, Soheili V. Review on plant antimicrobials: a mechanistic viewpoint. Antimicrob Resist Infect Control 2019;8:118.

30. Joshi DR, Nisha Adhikari. Benefit of cow urine, milk, ghee, curd, and dung versus cow meat. Acta Sci Pharm Sci 2019;3.8:169-75.

31. Sindhuja S, Prakruthi M, Manasa R, Naik R S, Shivananjappa M. Health benefits of ghee (clarified butter) - a review from ayurvedic perspective. IP J Nutr Metab Health Sci 2020;3(3):64-72.

32. Hamouda RA, Hussein MH, Abo-Elmagd RA, Bawazir SS. Synthesis and biological characterization of silver nanoparticles derived from the cyanobacterium Oscillatoria limnetica. Sci Rep 2019;9(1):13071.

33. Al-Bakri GA, Afifi FU. Evaluation of antimicrobial activity of selected plant extracts by rapid XTT colorimetry and bacterial enumeration. J Microbiol Meth 2007;68:19-25.

34. Pahal V, Kaur A, Dadhich KS. Effect of combination therapy using cow (Bos indicus) urine distillate and some indian medicinal plants against selective pathogenic gram-negative bacteria. Int J Pharm Sci Res 2017;8(5):2134-42.

35. Shikha S, Chaudhuri SR, Bhattacharyya MS. Facile one pot greener synthesis of sophorolipid capped gold nanoparticles and its antimicrobial activity having special efficacy against Gram Negative Vibrio cholerae. Sci Rep 2020;10(1):1463.

36. Ray SC, Sagha A, Jana NR, Sarkar R. Fluorescent carbon nanoparticles: synthesis, characterization, and bioimaging application. J Phys Chem C 2009;43:18546-51.

37. Lehrer RI, Rosenman M. Ultrasensitive assays for endogenous antimicrobial polypeptides. J Microbiol Meth 1991;137:167-73.

38. Dhandapani K, Venugopal K, Kumar JV. Ecofriendly and green synthesis of carbon nanoparticles from rice bran: characterization and identification using image processing technique. Int J Plast Technol 2019;23:56-66.

39. Roshni V, Gujar V, Pathan H, Islam S, Tawre M, Pardesi K, et al. Bioimaging applications of carbon dots (C. dots) and its cystamine functionalization for the sensitive detection of Cr(VI) in aqueous samples. J Fluoresc 2019;29(6):1381-92.

40. Zhao C, Wang X, Wu L, Wu W, Zheng Y, Lin L, et al. Nitrogen-doped carbon quantum dots as an antimicrobial agent against Staphylococcus for the treatment of infected wounds. Colloids Surf B Biointerfaces 2019;179:17-27.

41. Gao Z, Yang D, Wan Y, Yang Y. One-step synthesis of carbon dots for selective bacterial inactivation and bacterial differentiation. Anal Bioanal Chem 2020;412(4):871-80.

42. Shah SM, Rezaei B, Ensafi AA, Etemadifar Z. An ancient plant for the synthesis of a novel carbon dot and its applications as an antibacterial agent and probe for sensing of an anti-cancer drug. Mater Sci Eng C Mater Biol Appl 2019;98:826-33.

43. Singh SS, Bairagi PK, Verma N. Candle soot-derived carbon nanoparticles: an inexpensive and efficient electrode for microbial fuel cells. Electrochimica Acta 2018;264:119-27.

44. Rajeshwari P, Dey TK. Novel HDPE nanocomposites containing aluminum nitride (nano) particles: micro-structural and nano-mechanical properties correlation. Mater Chem Phys 2017;190:175-86.

45. Mohanty B, Verma AK, Claesson P, Bohidar HB. Physical and antimicrobial characteristics of carbon nanoparticles prepared from lamp soot. Nanotechnology 2007;18:445102.

46. Prasad KS, Chuang MC, Ho JA. Synthesis, characterization, and electrochemical applications of carbon nanoparticles derived from castor oil soot. Talanta 2012;188:445-9.

47. Dong X, Liang W, Meziani MJ, Sun YP, Yang L. Carbon dots as potent antimicrobial agents. Theranostics 2020;10(2):671-86.

48. Yang C, Mamouni J, Tang Y, Yang L. Antimicrobial activity of single-walled carbon nanotubes: length effect. Langmuir 2010;26(20):16013-9.

49. Mocan T, Matea CT, Pop T, Mosteanu O, Buzoianu AD, Suciu S, et al. Carbon nanotubes as anti-bacterial agents. Cell Mol Life Sci 2017;74(19):3467-79.

50. Liu S, Wei L, Hao L, Fang N, Chang MW, Xu R, et al. Sharper and faster ''nano darts'' kill more bacteria: a study of antibacterial activity of individually dispersed pristine single-walled carbon nanotube. ACS Nano 2009;3:3891-02.

51. Varghese S, Kuriakose S, Jose S. Antimicrobial activity of carbon nanoparticles isolated from natural sources against pathogenic GramNegative and Gram-Positive bacteria. J Nanosci 2013; Article ID 457865:1-5.

52. Aloysius C, Varghese AA, Pattekkal Ali S, Sukirtha TH, Aloysius Sabu N, Cyriac J, et al. Antibacterial activity of carbon nanoparticles isolated from chimney soot. IET Nanobiotechnol 2019;13(3):316-9.

53. Su S, Shelton CB, Qiu J. Proceedings of the ASME 2013 International Mechanical Engineering Congress and Exposition November 15-21, 2013, San Diego, CA, 2013.

54. Anand A, Unnikrishnan B, Wei SC, Chou CP, Zhang LZ, Huang CC. Graphene oxide and carbon dots as broad-spectrum antimicrobial agents - a minireview. Nanoscale Horiz 2019;4(1):117-37.

55. Jiang YW, Gao G, Zhang X, Jia HR, Wu FG. Antimicrobial carbon nanospheres. Nanoscale 2017;9(41):15786-95.

56. Zare-Zardini H, Ahmad A, Mehdi S, Ahmad A. Studying of antifungal activity of functionalized multiwalled carbon nanotubes by microwave-assisted technique.Surf Interface Anal 2012;3:1-5.

57. Simmons TJ, Lee SH, Park TJ, Hashim DP, Ajayan PM, Linhardt RJ. Antiseptic single wall carbon nanotube bandages. Carbon 2009;47:1561-4.

58. Amiri A, Zardini HZ, Shanbedi M, Maghrebi M, Baniadam MBT. Efficient method for functionalization of carbon nanotubes by lysine and improved antimicrobial activity and water-dispersion. Mater Lett 2012;72:153-6.

59. Arias LR, Yang L. Inactivation of bacterial pathogens by carbon nanotubes in suspensions. Langmuir 2009;25:3003-12.

60. CDC, 2019. Available via Biggest-Threats.html

61. Chen J, Andler SM, Goddard JM, Nugen SR, Rotello VM. Integrating recognition elements with nanomaterials for bacteria sensing. Chem Soc Rev 2017;46(5):1272-83.

62. Dizaj SM, Mennati A, Jafari S, Khezri K, Adibkia K. Antimicrobial activity of carbon-based nanoparticles. Adv Pharm Bull 2015;5:19-23.

63. Rennie PR. Current and future challenges in the development of antimicrobial agents. Springer-Verlag, Berlin, Germany, pp 45-65, 2012.

64. Arora S, Kaur H, Kumar R, Kaur R, Rana D, Rayat CS, et al. In vitro cytotoxicity of multiwalled and single walled carbon nanotubes on human cell lines. Fullerene Nanotubes Carbon Nanostruct 2015;23:377-82.

65. Mehta VN, Jha S, Basu H, Singhal RK, Kailasa SK. One step hydrothermal approach to fabricate carbon dots from apple juice for imaging of mycobacterium and fungal cells. Sensors Actuators B Chem 2015;213:434-43.

66. Kasibabu BSB, D'souza SL, Jha S, Singhal RK, Basu H, Kailasa SK. One-step synthesis of fluorescent carbon dots for imaging bacterial and fungal cells. Anal Methods 2015;7:2373-8.

67. Leid J, Ditto A, Knapp A, Shah P, Wright B, Blust R. In vitro antimicrobial studies of silver carbine complexes: activity of free and nanoparticle carbene formulations against clinical isolates of pathogenic bacteria. J Antimicrob Chemother 2012;67:138-48.

68. Dong X, Awak MA, Tomlinson N, Tang Y, Sun YP, Yang L. Antibacterial effects of carbon dots in combination with other antimicrobial reagents. PLoS One 2017;12(9):e0185324.

69. Prasad K, Lekshmi G, Ostrikov K, Lussini V, Blinco J, Mohandas M, et al. Synergic bactericidal effects of reduced graphene oxide and silver nanoparticles against Gram-positive and Gram-negative bacteria. Sci Rep 2017; Available via PMC5431540/.

70. Ardekani SM, Dehghani A, Ye P, Nguyen KA, Gomes VG. Conjugated carbon quantum dots: potent nano-antibiotic for intracellular pathogens. J Colloid Interface Sci 2019;552:378-87.

71. Harroun SG, Lai JY, Huang CC, Tsai SK, Lin HJ. Reborn from the Ashes: turning organic molecules to antimicrobial carbon quantum dots. ACS Infect Dis 2017;3(11):777-9.

72. Tejwan N, Saini AK, Sharma A, Singh TA, Kumar N, Das J. Metal-doped and hybrid carbon dots: a comprehensive review on their synthesis and biomedical applications. J Control Release 2021;10(330):132-50.

73. Dugam S, Nangare S, Patil P, Jadhav N. Carbon dots: a novel trend in pharmaceutical applications. Ann Pharm Fr 2021;79(4):335-45.

74. Guo FN, Wang YT, Wu N, Feng LX, Zhang HC, Yang T, et al. Carbon nitride nanoparticles as ultrasensitive fluorescent probes for the detection of α-glucosidase activity and inhibitor screening. Analyst 2021;7(3):1016-22.

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