Research Article | Volume: 1, Issue: 2, July-Aug, 2013
Gastroenteritis with Eschericha coli in pediatric hospital in N'Djamena-Chad
Gastroenteritis with Escherichia coli diarrhea is severe and recurrent fatal diseases in children under 5 years especially in Africa. A prospective study of acute diarrhea was conducted at the General Hospital of National Reference of N'Djamena (HGRN). The aim of this study was to investigate the epidemiological, clinical and evolutionary characteristics of acute diarrhea with enteropathogenic Escherichia coli. This study involved 167 children aged 0-2 years having seen in outpatients or hospitalized for acute diarrhea in children. Stool samples were collected and analyzed according to standard procedures of clinical microbiology. Identification of pathotypes of E. coli was performed by Nonavalents serum agglutination, Trivalent I, Trivalent II, Trivalent III and mixture IV. Antimicrobial susceptibility was performed by disk diffusion method in solid medium according MH Kirby Bauer. Examination of 167 stool showed 26 cases (15.56%) bloody, 141 cases (84.83%), mucous, 122 cases (73.05%) and liquid 45 cases (26.94%) soft. The enteropathogenic Escherichia coli (EPEC) were detected in 58 cases (34.73%). On the other hand parasites (16.76%) were identified to direct microscopic observation that Trichomonas intestinalis 13 cases (7.78%), Giardia intestinalis 9 cases (5.38%), Entamoeba histolytica 6 cases (3.59%) and cases of intestinal candidiasis (15.56%). The sensibility of origins in antibiotics was varied, 89.65 % of the ECEP, were resistant in Sulpha drugs, 56% were it towards beta-lactamines. An average rate of 26% of resistance was observed by the families of fluoroquinolones and quinolones. This study allowed us not only to include the characteristics of diarrheas at the children of less than two years, but also, the necessity of an effective coverage as well as the implementation of a program of prevention against the diarrheas of the children in a hospital environment.
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