Published:  Jun 20, 2015DOI: 10.7324/JABB.2015.3301
The effect of different light qualities (white, blue, green and red light) on the accumulation of phycobiliproteins was studied in Anabaena circinalis. The results also showed that responding to different light, this strain change the composition of their light-harvesting pigments, phycoerythrin (PE) and phycocyanin (PC). Photosynthetic pigment’s content (PE, PC and APC) were high in white light, phycoerythrin (31.53 µgmg-1), phycocyanin (135.01 µgmg-1) and allophycocyanin (35.92 µgmg-1) after 15 days of growth. In contrast, the lowest phycocyanin was found under blue light (20.01 µgmg-1). Red light favoured the accumulation of phycoerythrin (30.29 µgmg-1) than blue and green but lower than white light. Increasing awareness of harmful effects of synthetic compounds and towards the usage of natural products have led to the exploitation of microalgae as a source of natural pigments. The findings revealed that phycobiliproteins composition and colour of strain drastically changed in response to light quality through complementary chromatic adaptation. Phycocyanin content was significantly correlated with fluorescent white light indicating its important role in cyanobacteria.
Ojit S.K., Indrama Th., Gunapati O., Avijeet S.O., Subhalaxmi S.A., Silvia Ch., Indira D.W., Romi Kh., Minerva Sh., Thadoi D.A., Tiwari O.N., Sharma G.D. The response of phycobiliproteins to light qualities in Anabaena circinalis . J App Biol Biotech. 2015; 3 (03): 001-006.
1. Patel A, Sandhya M, Pawar R, Ghosh PK. Purification and characterization of C phycocyanin from cyanobacterial species of marine and freshwater habitat. Protein Expression and Purification. 2005; 40(2): 248-255.
2. Viskari PJ, Colyer CL. Rapid extraction of phycobiliproteins from cultured cyanobacteria samples. Analytical Biochemistry. 2003; 319:263-271.
3. Shrivastav A, Mishra SK, Mishra S. Polyhydroxyalkanoate (PHA) synthesis by Spirulina subsalsa from Gujarat coast of India. International Journal of Biological Macromolecules. 2010; 46(2):255-260.
4. Spolaore P, Joannis-Cassan C, Duran E, Isambert A. Commercial applications of microalgae. Journal of Bioscience and Bioengineering. 2006;101:87-96.
5. Mishra S. Phycobiliproteins from microalgae. In: Tewari A, editor. Recent advances on applied aspects of Indian marine algae with reference to Global scenario-2, Bhavnagar, India: CSMCRI; 2007, p. 273-284.
6. Soni B, Kalavadia B, Trivedi U, Madamwar D. Extraction, purification and characterization of phycocyanin from Oscillatoria quadripunctulata isolated from the rocky shores of Bet-Dwarka, Gujarat, India. Process Biochemistry. 2006; 41:2017-2023.
7. Patel A, Pawar R, Mishra S, Sonawane S, Ghosh PK. Kinetic studies on thermal denaturation of C-phycocyanin. Indian Journal of Biochemistry and Biophysics. 2004; 41:254-257.
8. Mishra SK, Shrivastav A, Mishra S. Effect of preservatives for food grade C-PC from Spirulina platensis. Process Biochem. 2008; 43:339-345.
9. Mishra SK, Shrivastav A, Mishra S. Effect of preservatives for food grade C-phycoerythrin isolated from marine cyanobacteria Pseudanabaena sp. International Journal of Biological Macromolecules. 2010; 47:597-602.
10. Eriksen NT. Production of phycocyanin-a pigment with applications in biology, biotechnology, foods and medicine. Applied Microbiology and Biotechnology. 2008; 80:1-14.
11. Singh NK, Parmar A, Sonani RR, Madamwar D. Isolation, identification and characterization of novel thermotolerant Oscillatoria sp. N9DM: change in pigmentation profile in response to temperature. Process Biochemistry. 2012; 47(12):2472-2479.
12. Madhyastha HK, Vatsala TM. Pigment production in Spirulina fussiformis in different photophysical conditions. Biomolecular Engineering. 2007; 24(3):301-305.
13. Stowe-Evans EL, Kehoe DM. Signal transduction during light-quality acclimation in cyanobacteria: a model system for understanding phytochrome-response pathways in prokaryotes. Photochemical and Photobiological Sciences. 2004; 3(6):495-502.
14. Ajayan KV, Selvaraju M, Thirugnanamoorthy K. Enrichment of chlorophyll and phycobiliproteins in Spirulina platensis by the use of reflector light and nitrogen sources: an in-vitro study. Biomass and Bioenergy. 2012; (47):436-441.
15. Barsanti L, Gualtieri P. Algae: anatomy, biochemistry, and biotechnology. CRC Press: Taylor and Francis Group; 2006.
16. Wang CY, Fu CC, Liu YC. Effects of using light-emitting diodes on the cultivation of Spirulina platensis. Biochemical Engineering Journal. 2007; 37:21-25.
17. Kagawa T, Suetsugu N. Photometrical analysis with photosensory domains of photoreceptors in green algae. FEBS Letters. 2007; 581(3):368-374.
18. Prasanna R, Dhar DW, Domnic TK, Tiwari ON, Singh PK. Isolation and characterization of phycobiliprotein rich mutant of cyanobacterium Synechocystis sp. Acta Biologica Hungarica. 2003; 54:113-120.
19. Desikachary TV. Cyanophyta. ICAR Monograph on Algae. New Delhi, India: ICAR; 1959.
20. Stanier RY, Kunisawa R, Mandel M, Cohen-Bazire G. Purification and properties of unicellular blue-green algae (order Chroococcales). Bacteriological Reviews. 1971; 35(2):171-205.
21. Bennett A, Bogorad L. Complementary chromatic adaptation in a filamentous blue-green alga. Journal of Cell Biology. 1973; 58(2):419-435.
22. Bell PRF, Fu FX. Eï¬€ect of light on growth, pigmentation and N2 ï¬xation of cultured Trichodesmium sp. from the Great Barrier Reef lagoon. Hydrobiologia. 2005; 543:25-35.
23. Ashokkumar P, Anand N. Studies on growth and phycobilin pigments of the cyanobacterium Westiellopsis iyengarii. International Journal of Biotechnology and Biochemistry. 2010; 6:315-323.
24. Mishra SK, Shrivastav A, Maurya RR, Patidar SK, Haldar S, Mishra S. Effect of light quality on the C-phycoerythrin production in marine cyanobacteria Pseudanabaena sp. isolated from Gujarat coast, India. Protein Expression and Purification. 2012; 81(1):5-10.
25. Vijaya V, Anand N. Blue light enhance the pigment synthesis in cyanobacterium Anabaena ambigua Rao (Nostacales). ARPN Journal of Agricultural and Biological Science. 2009; 4(3):36-43.
26. Hemlata AE, Fatma T. Screening of cyanobacteria for phycobiliproteins and effect of different environmental stress on its yield. Bulletin of Environmental Contamination and Toxicology. 2009; 83(4):509-515.
27. Rodriguez H, Rivas J, Guerrero MG, Manuel-Losada M. Enhancement of phycobiliprotein production in nitrogen-fixing cyanobacteria. Journal of Biotechnology. 1991; 20:263-270.
28. Lemasson C, De Marsac NT, Cohen-Bazire G. Role of allophycocyanin as a light-harvesting pigment in cyanobacteria. Proceedings of the National Academy of Sciences, USA. 1973; 70:3130-3133.
29. Poza-Carrion C, Fernadez-Valiente E, Fernadez-Pinas F, Leganes F. Acclimation of photosynthetic pigments and photosynthesis of the cyanobacterium Nostoc sp. Strain UAM 206 to combined fluctuations of irradiance, pH and inorganic carbon availability. Journal of Plant Physiology. 2001; 158:1455-1461.
30. Ranjitha K, Kaushik BD. Influence of environmental factors on accessory pigments of Nostoc muscorum. Indian Journal of Microbiology. 2005; 45:67-69.
31. Franklin LA, Krabs G, Kuhlenkamp P. Blue light and UV radiation control the synthesis of mycosporine like amino acids in Chondrus crispus (Floridiophyceae). Journal of Phycology. 2002; 37:257-270.
32. Godinez-Ortega JL, Snoeijs P, Robledo D, Freile-Pelegrin Y, Pedersen M. Growth and pigment composition in the red algae Halymenia floresii cultured under different light qualities. Journal of Applied Phycology. 2008; 20:253-260.
33. Tsekos I, Niell FX, Aguilera J, Lopez-Fiueroa F, Delivopoulos SG. Ultrastructure of vegetative gametophytic cells of Porphyra leukostica (Rhodophyta) grown in red, blue and green light. Phycological Research. 2002; 50:251-264.
34. Beguin S, Guglielmi G, Rippka R, Cohen-Bazire G. Chromatic adaptation in a mutant of Fremyella diplosiphon incapable of phycoerythrin synthesis. Biochimie. 1985; 67:109- 117.
35. Glazer AN. Phycobilisome: a macromolecular complex optimized for light energy transfer. Biochimica et Biophysica Acta. 1984; 768:29-51.
36. Eldin MJ, Kanhaiya K, Debabrata D. Physico-chemical parameters optimization and purification of phycobiliproteins from the isolated Nostoc sp. Bioresource Technology. 2014; 166:541-547.
37. Bordowitz JR, Montgomery BL. Photoregulation of cellular morphology during complementary chromatic adaptation requires sensor-kinase-class protein RcaE in Fremyella diplosiphon. Journal of Bacteriology. 2008; 190(11):4069-4074.
38. Gan F, Zhang S, Rockwell NC, Martin SS, Lagarias JC, Bryant DA. Extensive re-modelling of a cyanobacterial photosynthetic apparatus in far-red light. Science. 2014; 345:1312-1317.
39. Gutu A, Kehoe DM. Emerging perspectives on the mechanisms, regulation, and distribution of light colour acclimation in cyanobacteria. Molecular Plant. 2012; 5:1-13.
40. Kehoe DM, Gutu A. Responding to colour: the regulation of complementary chromatic adaptation. Annual Review of Plant Biology. 2006; 57:127-150.
41. Korbee N, Figueroa FL, Aguilera J. Effect of light quality on the accumulation of photosynthetic pigments, proteins and mycosporine-like amino acids in the red alga Porphyra leucosticta (Bangiales, Rhodophyta). Journal of Photochemistry and Photobiology B: Biology. 2005; 80(2):71-78.
42. Grossman AR. A molecular understanding of complementary chromatic adaptation. Photosynthesis Research. 2003; 76:207-215.
43. Bogorad L. Phycobiliproteins and complementary chromatic adaptation. Annual Review of Plant Physiology. 1975; 26:369-401.
44. Vogelmann TC, Scheibe J. Action spectra for chromatic adaptation in the blue-green alga Fremyella diplosiphon. Planta. 1978; 143:233-239.
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