Research Article | Volume 9, Supplement 1, September, 2021

Osteogenic potential of primary stem cells derived from the human dental pulp is enhanced by carboxymethyl cellulose/chitosan scaffold doped with wollastonite particles

Dannie Macrin Vivek Narayanan Arikketh Devi   

Open Access   

Published:  Sep 20, 2021

DOI: 10.7324/JABB.2021.95.1s1
Abstract

The success of any scaffold assisted regenerative therapy relies on two major factors; a conducive scaffold material and versatile, viable stem cells. In our study, we suggest chitosan (CS)/carboxymethylcellulose (CMC) scaffold functionalized with wollastonite (WS) (CaSiO3 ) particles as a conducive scaffold material which mimics the porous structure of the bone and promotes osteogenesis. We have paired this scaffold material with stem cells derived from dental pulp (DPSCs) which is an ideal cell source with a high percentage of stem cells and have a natural inclination toward forming hard calcified tissues. In our experiments, the cells isolated from human dental pulp produced a cell population with high percentage of cells positive for mesenchymal markers CD73 and CD90. The DPSCs were able to differentiate into osteoblasts when induced with media supplemented with β-glycerophosphate, ascorbic acid, and dexamethasone. Furthermore, physicochemical analysis of CS/CMC/WS scaffold showed that it formed a highly porous structure conducive to cell growth, penetration, and nutrient uptake. Furthermore, CS/CMC/WS scaffold promoted osteogenic differentiation of DPSCs. We propose CS/CMS/WS scaffold paired with DPSCs as an effective system for bone regeneration.


Keyword:     Adult stem cells mesenchymal markers bone regeneration osteogenic differentiation


Citation:

Macrin D, Narayanan V, Devi A. Osteogenic potential of primary stem cells derived from the human dental pulp is enhanced by carboxymethyl cellulose/chitosan scaffold doped with wollastonite particles. J Appl Biol Biotech, 2021;9(S1):1-6

Copyright: Author(s). This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.

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