Research Article | Volume: 1, Issue: 2, July-Aug, 2013

Isolation and characterization of Soybean DREB 3 transcriptional activator

Sarosha Nasreen J. Amudha S. S. Pandey   

Open Access   

Published:  Aug 30, 2013

DOI: 10.7324/JABB. 2013.1202

Cotton (Gossypium hirsutum) is an important cash crop and its productivity is significantly hampered by Abiotic stresses, such as drought and high salinity. High salt imposes negative impacts on growth, agronomy traits, seed quality and quantity and thus reduces the yield of cotton. To cope with salt stress, cotton plant has to develope several tolerance mechanisms, including: (i) maintenance of ion homeostasis; (ii) adjustment in response to osmotic stress; (iii) restoration of osmotic balance; and (iv) other metabolic and structural adaptations. The regulatory network for abiotic stress responses in higher plants has been studied extensively in model plants such as Arabidopsis thaliana. A novel soybean DREB (dehydration-responsive element-binding protein) functions as an important transcriptional activator and may be useful in improving plant tolerance to abiotic stresses in plants. Based on its similarity with AP2 domains, DREB3 was classified into A-5 subgroup in DREB subfamily in AP2/EREBP family. A DREB orthologue, DREB3 ,a 528 bp fragment of DREB3 containing the DNA-binding domain was amplified using the primer pair 5# -CCCTCTAGAGAATTCATGGCGAAACCCAGCAGC-3# (forward) and 5#-CCCCTCGAGCGGCATTTCCGGCACATA-3# (reverse). The amplified product was electrophoresed using 1.5% agarose gel and the fragment was eluted. The eluted fragment was quantified using nanodrop and then subjected to poly -AAA -tailing and cloned into pGEM-T easy vector kit. The soybean DREB 3 gene functions as an important transcriptional activator and may be useful in improving of plant tolerance to abiotic stress in cotton plants.

Keyword:     Soybean DREB 3 abiotic stresses Gossypium hirsutum.

Copyright: Author(s). This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.

HTML Full Text

1. Bartels D, Sunkar R. Drought and salt tolerance in plants. Critical Reviews in Plant Sciences. 2005; 24: 23–58.

2. Benedict C, Skinner JS, Meng R, Chang Y, Bhalerao R,Huner NA, Finn CE, Chen TH, Hurry V. The CBF1-dependent low temperature signaling pathway, regulon and increase in freeze tolerance are conserved in populus spp. Plant, Cell and Environment. 2006; 29:1259–1272.

3. Blum A. Crop responses to drought and the interpretation of adaptation. Plant Growth Regulation. 1996; 20: 135–148.

4. Chen JQ, Dong Y, Wang YJ, Liu Q, Zhang JS. 2003. An AP2/EREBP-type transcript-factor gene from rice is cold-inducible and encodes a nuclear-localized protein. Theoretical and Applied Genetics; 107: 972–979.

5. Chen L, Zhang Z, Liang H, Liu H, Du L, Xu H, Xin Z. Overexpression of TiERF1 enhances resistance to sharp eyespot in transgenic wheat. J Exp Bot. 2008; 59:4195–4204.

6. Chen M, Xu Z, Xia L, Li L, Cheng X, Dong J, Wang Q, Ma Y.Cold-induced modulation and functional analyses of the DREbinding transcription factor gene, GmDREB3, in soybean (Glycine max L). J Exp Bot. 2009; 60:121–135.

7. Fowler S and Thomashow. MF. Arabidopsis transcriptome profiling indicates that multiple regulatory pathways are activated during cold acclimation in addition to the CBF cold response pathway. Plant Cell. 2002; 14: 1675-1690.

8. Gutterson N, Reuber TL. Regulation of disease resistance pathways by AP2/ERF transcription factors. Curr Opin Plant Biol. 2004; 7:465–471.

9. Guo A, He K, Liu D, Bai S, Gu X. DATF: a database of Arabidopsis transcription factors. Bioinformatics. 2005; 21:2568–2569.

10. Hong JP, Kim WT. Isolation and functional characterization of the Ca-DREBLP1 gene encoding a dehydration-responsive element binding-factor-like protein 1 in hot pepper (Capsicum annuum L. cv. Pukang). Planta. 2005; 220: 875–888.

11. Ito Y, Katsura K, Maruyama K, Taji T, Kobayashi M, Seki M,ShinozakiK,Yamaguchi-Shinozaki K. Functional analysis of 012 Nasreen et al. / Journal of Applied Biology & Biotechnology 1 (02); 2013: 009-012 rice DREB1/CBF-type transcription factors involved in coldresponsive gene expression in transgenic rice. Plant Cell Physiol. 2006; 47:141–153.

12. Iwasaki T, Kiyosue T, Yamaguchi-Shinozaki K, Shinozaki K.The dehydration- inducible Rd17 (Cor47) gene and its promote region in Arabidopsis thaliana. Plant Physiol. 1997; 115:1287–1289.

13. Kasuga M, Miura S, Shinozaki K and Yamaguchi-Shinozaki K .A combination of the Arabidopsis DREB1A gene and stressinducible rd29A promoter improved drought- and lowtemperature stress tolerance in tobacco by gene transfer. Plant Cell Physiol. 2004; 45: 346-350.

14. Li Y, Su X, Zhang B, Huang Q, Zhang X, Huang R. Expression of jasmonic ethylene responsive factor gene in transgenic poplar tree leads to increased salt tolerance. Tree Physiol. 2009; 29:273–279.

15. Liu N, Zhong NQ, Wang GL, Li LJ, Liu XL, He YK, Xia GX. Cloning and functional characterization of PpDBF1 gene encoding a DRE-binding transcription factor from Physcomitrella patens. Planta. 2007; 226:827–838.

16. Nakano T, Suzuki K, Fujimura T, Shinshi H. Genome-wide analysis of the ERF gene family in Arabidopsis and rice. Plant Physiol. 2006; 140:411–432

17. Navarro M, Marque G, Ayax C, Keller G, Borges JP, Marque C, Teulières C. Complementary regulation of four eucalyptus CBF genes under various cold conditions. J Exp Bot. 2009; 60:2713–2724

18. Qin F, Sakuma Y, Li J, Liu Q, Liu YQ, Shinozaki K,Yamaguchi Shinozaki K. 2004.

19. Cloning and functional analysis of a novel DREB1/CBF transcription factor involved in cold-responsive gene expression in Zea mays L. Plant and Cell Physiology; 45: 1042–1052.

20. Qin QL, Liu JG, Zhang Z, Peng RH, Xiong AS. Isolation, optimization, and functional analysis of the cDNA encoding transcription factor RdreB1 in Oryza sativa L. Mol Breed. 2007; 19:329–340

21. Seki M, Narusaka M, Abe H, Kasuga M. Monitoring the expression `pattern of 1300 Arabidopsis genes under drought and cold stresses by using a full-length cDNA microarray. Plant Cell 2001; 13: 61-72.

22. Shen YG, Zhang WK, He SJ, Zhang JS, Liu Q, Chen SY. An EREBP/AP2 type protein in Triticum aestivum was a DREbinding transcription factor induced by cold, dehydration and ABA stress. Theoretical and Applied Genetics. 2003; 106; 923–930.

23. Tardi eu F. Plant tolerance to water deficit: physical limits and possibilities for progress. Comptes Rendus Geoscience. 2005;337; 57–67.

24. Umezawa T, Fujita M, Fujita Y, Yamaguchi-Shinozaki K, Shinozaki K. Engineering drought tolerance in plants:discovering and tailoring genes to unlock the future. Current Opinion in Biotechnology. 2006; 17: 113–122

25. Vij S, Tyagi AK. Emerging trends in the functional genomics of the abiotic stress response in crop plants. Plant Biotechnology Journal. 2007.; 5: 361–380.

26. Vinocur B, Altman A. Recent advances in engineering plant tolerance to abiotic stress: achievements and limitations. Current Opinion in Biotechnology. 2005; 16: 123–132.

27. Volkov RA, Panchuk II and Schoffl F. Heat-stress-dependency and developmental modulation of gene expression: the potential of house-keeping genes as internal standards in mRNA expression profiling using real-time RT-PCR. J. Exp. Bot. 2003; 54: 2343-2349.

28. Wang H, Datla R, Georges F, Loewen M, Cutler AJ. Promoters from kin1 and cor6.6, two homologous Arabidopsis thaliana genes: transcriptional regulation and gene expression induced by low temperature, ABA, osmoticum and dehydration. Plant Mol Biol. 1995; 28:605–617

29. Xu ZS, Chen M, Li LC, Ma YZ. Functions of the ERF transcription factor family in plants. Botany. 2008;86:969–977

30. Zhuang J, Cai B, Peng RH, Zhu B, Jin XF, Xue Y, Gao F, Fu XY, Tian YS, Zhao W, Qiao YS, Zhang Z, Xiong AS, Yao QH. Genome-wide analysis of the AP2/ERF gene family in Populus trichocarpa. Biochem Biophys Res Commun. 2008; 371:468–474.

31. Zhuang J, Peng RH, Cheng ZM, Zhang J, Cai B, Zhang Z, Gao F, Zhu B, Fu XY, Jin XF, Chen JM, Qiao YS, Xiong AS, Yao QH. Genome-wide analysis of the putative AP2/ERF family genes in Vitis vinifera. Sci Hortic. 2009; 123:73–81

Article Metrics

953 Absract views 326 PDF Downloads 1279 Total views

Related Search

By author names

Citiaion Alert By Google Scholar

Similar Articles