Research Article | Volume: 2, Issue: 6, Nov-Dec, 2014

Enzymes and qualitative phytochemical screening of endophytic fungi isolated from Lantana camara Linn. Leaves

Mbouobda Hermann Desire Fotso Bernard Muyang Rosaline Forsah Chiatoh Thaddeus Assang Omokolo Ndoumou Denis   

Open Access   

Published:  Dec 27, 2014

DOI: 10.7324/JABB.2014.2601

Plants harbor endophytes, capable of producing metabolites and enzymes with functions similar to host plant extracts. An inventory of Lantana camara’s endophytes could better explain the role played by extracts of this plant in its defense functions against pathogens. This study aimed at isolating and screening endophytic fungi from L. camara leaves for amylases, lipases and laccases production and for the production of some secondary metabolites using standard procedures. In-vitro culture techniques with Potato Dextrose Agar (PDA) as culture medium were used to isolate endophytes from L. camara leaf tissues. Isolates identification were done using macroscopic and microscopic characteristics. Three endopyhtic fungi were isolated and labeled LI, LII, and LIII; LI and LII had a slimy texture and produced no reproductive structures (spores, conidia, septate hyphae etc) hence identified as Mycelia sterilia. LIII was white, with an uneven surface growth, cottony dry aerial mycelium, visible hyphae with little branching and individual hyphae were septate, hence a Geotrichium sp. All isolates produced amylases and lipases while LIII solely produced laccase. The extract of LIII showed the presence of tannins and cardiac glycosides and absence of phenols and saponnins. The anti pathogenic roles of L. camara extract are thanks to the presence of endophytes in its tissues which produce enzymes that mediate plant defense functions as well as secondary metabolites with anti-pathogenic properties.

Keyword:     Endophytic fungiextracellular enzymessecondary metabolitesplant defense.


Mbouobda Hermann Desire, Fotso Bernard, Muyang Rosaline Forsah, Chiatoh Thaddeus Assang, Omokolo Ndoumou Denis. Enzymes and qualitative phytochemical screening of endophytic fungi isolated from Lantana camara Linn. Leaves. J App Biol Biotech. 2014; 2 (06): 001-006. DOI: 10.7324/JABB.2014.2601

Copyright: Author(s). This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.

HTML Full Text

1. Sunitha V, Nirmala D, Srinivas C. Extracellular enzymatic activity of endophytic fungal strains isolated from medicinal plants. World Journal of Agricultural Sciences.2013;9(1):01-09.

2. Al-mahi I, Ietidal A and Eihab I. Antibacterial activity of endophytic fungi extracts from the medicinal plant Kigelia Africana. Egyptian Academic Journal of Biological Sciences. 2013; 5(1): 1-9.

3. Selim K, El-Beih A, Abdel-Rahman T, El-Diwany A. Biology of Endophytic Fungi. Current Research in Environmental & Applied Mycology. 2012; 2(1): 31-82.

4. Saba H, Garima G, Shreya A, Harpreet K. Lytic Enzymes of Trichoderma: Their Role in Plant Defense. International Journal of Applied Research and Studies. 2014; 3:2-5.

5. Higuchi T. Microbial degradation of lignin, role of lignin peroxidase, manganese peroxidase, and laccase. Proceedings of Japan Academy. 2004; B80: 204–214.

6. Ferrar P, Barbere S, Ginger M, and Walker R. Laccase- new roles for an old enzyme. New Zealand Bioscience.1995; 3: 7-13.

7. Mayer A and Harel E. Polyphenol oxidases in plants. Phytochemistry.1979; 18: 193-215.

8. Hari O, Anjana S, Naseer M and Santosh K. Phytochemical screening and elemental analysis in different plant parts of Uraria picta Desv.: A Dashmul species. Journal of Chemical and Pharmaceutical Research. 2014; 6(5):756-760.

9. Phyllis D. Coley. Costs and benefits of defense by tannins in a neotropical tree. Oecologia.1986; 70:238-241.

10. Rebecca E, Robert J, and John T. Feeny revisited: condensed tannins as anti-herbivore defences in leaf-chewing herbivore communities of Quercus. Ecological Entomology.2004; 29: 174–187.

11. James Hamuel Doughari. Phytochemicals: Extraction Methods, Basic Structures and Mode of Action as Potential Chemotherapeutic Agents. Department of Microbiology, School of Pure and Applied Sciences, Federal University of Technology, Yola Nigeria.

12. Publisher InTech, Published online 21, March, 2012, Published in print edition March, 2012, 33p.

13. Ellen D, Ellen L, Danny G, and Guy S. Novel Advances with Plant Saponins as Natural Insecticides to Control Pest Insects. Pest Technology. 2007; 96-105.

14. Osbourn A. Saponins and plant defence - a soap story. Trends Plant Science.1996; 1:4-9.

15. Figen Mert-TŸrk. Saponins versus plant fungal pathogens. Journal of Cell and Molecular Biology. 2006; (13) 5:13-17.

16. Hollman A. Plants and cardiac glycosides. Br Heart. 1985; (7) 54: 258-261.

17. Sanjeeb K, Gaurav K, Loganathan K, Kokati V. and Bhaskara R. A review on medicinal properties of Lantana camara Linn. Research J. Pharm. and Tech. 2013; 5(6):711-715.

18. 17 Rezwana K, Saleem S, Iqbal C, Shakeel A and Aqeel A. Biodiversity of the endophytic fungi isolated from calotropis procera (ait.) R. BR Pak. J. Bot. 2007; 39(6): 2233-2239.

19. Heaton L.H. Pauley G.B. Two modified strains for use in oyster pathology. J. Fish Res. Board. 1969; 26: 707-709. In: Clark Geoge. Staining procedures, fourth edition (Williams and Wilkins), 1980, 512 p.

20. Nameirakpam N, Prabakaran J and Wahab F. Phytochemical analysis and enzyme analysis of endophytic fungi from Centella asiatica. Asian Pacific Journal of Tropical Biomedicine. 2012; 1280-1284.

21. Min-Yuan H, Wen-Chuan C, Hung-Chang H, Wen-Hsia C, and Wen-Hsin C. Identification of endophytic fungi of medicinal herbs of Lauraceae and Rutaceae with antimicrobial property. Taiwania. 2012; 57(3): 229-241.

22. Huang Y, Wang J, Li G, Zheng Z, and Su W. Antitumor and antifungal activities in endophytic fungi isolated from pharmaceutical plants Cephalataxus fortunei and Torreya grandis. Immunology and Medical Microbiology.2001; 31: 163-167.

23. Bourret TB, Kramer EK, Rogers JD, Glawe DA. Isolation of Geotrichum candidum pathogenic to tomato (Solanum lycopersicum) in Washington State. North American Fungi 2013; 8 (14): 1-7.

24. Jayasinghe C and Wettasinghe J. Cultural characteristics and reproductive morphology of Geotrichum sp.: A guide to distinguish Geotrichum from Rigidoporus microporus. Journal of the Rubber Research Institute of Sri Lanka.1998; 81, 23-28.

25. Choi Y, Hodgkiss I. Hyde K. Enzyme production by endophytes of Brucea javanica. Journal of Agricultural Technology. 2005; 1: 55-66.

26. Panuthai T, Sihanonth P, Piapukiew J, Sooksai S, Sangvanich P. Karnchanatat, African Journal of Microbiology Research. 2012; 6 (11): 2622-2638.

27. Pragathi D, Vijaya T, MouliK C, Anitha D. Diversity of fungal endophytes and their bioactive metabolites from endemic plants of Tirumala hills- Seshachalam biosphere reserve. African Journal of Biotechnology. 2013; 12 (27): 4317-4323.

28. Madhave and Lele. Laccase properties, use. BioResources.2009; 4(4): 1694-1717.

29. Huang WY, Cai YZ, Zhang Y. Natural phenolic compounds from medicinal herbs and dietary plants: Potential use for cancer prevention. Nutr Cancer. 2010; 62 (1): 1-20

Article Metrics
247 Views 140 Downloads 387 Total



Related Search

By author names

Similar Articles