Research Article | Volume: 8, Issue: 1, Jan-Feb, 2020

Partial purification and characterization of lectin from serum of American cockroach, Periplaneta americana

Reymend Encily Martin Yogita Raosaheb Channe   

Open Access   

Published:  Jan 10, 2020

DOI: 10.7324/JABB.2020.80110
Abstract

The partial purification and characterization of haemagglutinin (lectin) were carried out from the hemolymph of the adult American cockroach, Periplaneta americana. The hemolymph was drawn from cockroach and lectin was purified by a single-step method, using ammonium sulfate (NH4)2SO4 salt fractionation and gel filtration. Gel filtration showed two peaks. The Hemagglutination Activity (HA) was observed in the 20th fraction of the second peak. The purified lectin showed a molecular weight of 26.8kDa on Sodium Dodecyl Sulfate-Poly Acrylamide Gel Electrophoresis. The purified lectin showed an increase in HA at pH 7.5 and, subsequently, a sharp decline at pH 8. This indicates that HA was specific to a certain pH level. Similarly, an increase in HA was observed until 30°C, followed by a decline at 40°C. This indicates the heat labile nature of lectin. The HA showed a higher specificity to divalent Ca2+ and showed no specificity for Ba2+. It also showed a higher inhibition for sugar D-galactose and a least inhibition for D-lactose. The HA to vertebrate blood group showed a highest activity to goat Red Blood Cells (RBCs). The study concludes that carbohydrate-binding-specific lectin is important for recognition of the cell surface carbohydrate of invading pathogens.


Keyword:     Purification lectin sugar specificity pH temperature Periplaneta americana.


Citation:

Martin RE, Channe YR. Partial purification and characterization of lectin from serum of American cockroach, Periplaneta americana. J Appl Biol Biotech. 2020;8(01):59-63. DOI: 10.7324/JABB.2020.80110

Copyright: Author(s). This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.

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1. INTRODUCTION

Lectins have been purified from various insect species, such as fleshflies and cockroaches [1,2]. Some of them actively participate in self-defense by recognizing the polysaccharide chains on the surface of pathogens. Some insect lectins bind to microbial components such as lipopolysaccharide and activated prophenoloxidase (ProPO), and enhanced the cellular immune response by modulating hemocytes. The insect immune system activation mechanism was studied in Drosophila melanogaster, as a model organism [3,4]. Drosophila is estimated to have more than 30 C-type lectin genes [5,6]. However, very little is known about the participation of lectin in Drosophila immunity [7]. Undoubtedly, the presence of lectin in the hemolymph of insects appears to have an important function in insect immune defense system [8,9].

In invertebrates, including insects where they lack an antibody-based immunity, lectin probably plays a major role in nonself recognition, with several reports of endogenous serum lectin having opsonic activity, for invading pathogens [10,11]. Other than opsonization, lectin trigger ProPO activation pathway [12]. Arumugan, et al.[13] purified, characterized and identified humoral immune function on the induction of PO activity with the hemolymph lectin of Periplaneta americana. In this study, we examine in detail the hemolymph lectin from P. americana hemolymph, for its role in immune recognition and its properties.


2. MATERIALS AND METHODS

2.1. Insect Rearing

The cockroach, P. americana was collected from Godown’s and reared in the laboratory in a dark container. They were reared in room temperature 27 ± 1°C and 12:12 Total Light (LL): Total Dark (DD) cycle. All experiments were conducted at this room temperature unless specified.

2.2. Preparation of Hemolymph Sera

The insect hemolymph was collected from 40 cockroaches of the same sizes. Before collecting hemolymph, the individual cockroaches were first anesthetized by keeping them at low temperature for 20 minutes. Then, they were bled by severing hind legs with fine scissors, and hemolymph was collected in a sterile centrifuge tube containing phenyl thiourea crystals. Phenyl thiourea crystals were used to inhibit phenoloxidase activity. After 10 minutes, the tubes were centrifuged at 10,000 rpm at 4°C for 10 minutes to remove hemocytes. The supernatant was carefully removed and used as a serum for HA assay against goat’s Red Blood Cells (RBCs). The collected samples were tested immediately to prevent counterfeit negative results.

2.3. Isolation of Lectin: (Salting out of Serum Protein)

The lectin was isolated according to the procedure as described by Dorrah et al. [9].

2.4. Preparation of Erythrocyte Suspension and Assay of Hemagglutination Activity (HA)

Blood samples from goat and chicken were collected aseptically, each in an equal volume in heparin. Human blood (types A, B, and O) was collected in citrate/dextrose. The erythrocyte suspension and HA was determined by the procedure described in [14].

2.5. Effect of Divalent Cations

To study the effect of divalent cation for HA, the agglutination tests were performed as described by Richards et al. [15]. An aliquot of 100 μl of diluted serum was dialyzed for 24 hours at 4°C against 200 ml Tris Buffer Saline (TBS), pH 7, either without or with 20 mM Ca2+, 20 mM Ba 2+, 20 mM Mg2+, 20 mM Zn2+, 20 mM Hg2+, or 10 mM Ethylene Di-amine Tetra Acetic acid (EDTA) with 2 changes of buffer. HA assays were carried out by using goat RBCs washed in dialysis buffer.

2.6. Inhibition Assay

The binding of sugars to serum lectin was studied by competitively binding and measuring HA in the presence of goats RBCs. The method of inhibition followed in this study was as described by Ayyad et al. [14]. The sugars assessed in this study are monosaccharides (D-galactose, D-glucose, and D-fructose) and oligosaccharides (D-lactose). The minimum concentration of sugars for causing 50% inhibition (IC50) of HA was studied.

2.7. Effect of Temperature and pH

For the heat stability test, an aliquot of the protein sample was incubated in a heating block at different temperatures (10°C, 20°C, 30°C, and 40°C) for 30 minutes. The solution was cooled on ice immediately and then allowed to warm up to 27°C at room temperature, and thereafter the HA was assayed. The percentage of HA was calculated by dividing the activity of the sample with the activity of the control kept at temperature 27°C Room Temperature (RT) multiplied by 100.

For pH test, the lectin in buffer solutions with different pH values, i.e., 6–6.5 pH (0.2 M Citrate buffer) and 7–8 pH (0.2 M Tris-HCl buffer), was used. Equal volumes of protein sample and buffer solution were mixed and incubated at room temperature for 30 minutes. The solution was neutralized and the HA assay was performed. The hemagglutination titer of the protein sample was incubated in Phosphate Buffer Saline (PBS) (pH 7), which served as control. The percentage of HA was calculated by dividing the activity of the sample with the activity of the control of pH 7.5 multiplied by 100.

2.8. Determination of Protein Concentration

The total protein concentration was determined according to the method of Lowry et al. [16], using bovine serum albumin (BSA) dissolved in 0.15 M NaCl was used as a standard.

2.9. SDS-Poly Acrylamide Gel Electrophoresis

The SDS-PAGE of the isolated lectin was carried out according to the procedure described by Laemmil [17]. The electrophoresis was performed at a constant voltage of 200 V for 90 minutes. The molecular weight of the protein bands was determined using standard medium-range molecular markers: 97.4KDa-Phosphorylase-b; 66KDa-BSA; 43 kDa ovalbumin, 29kDa carbonic anhydrase, 20.1kDa soyabean trypsin inhibitor, 14.2kDa lysozyme.

Table 1: Purification of lectin from serum of American cockroach, P americana.

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Figure 1: The profile of gel filtration on Sephadex G 75 showing two peaks. Only the second peaks fraction 20 showed hemagglutinating Activity.

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3. RESULTS AND DISCUSSION

3.1. The Physico-Chemical Properties of the Isolated Lectins

In this study, a single-step purification procedure was used to purify lectin. The chromatographic step using column Sephadex G-75 was used to remove most of the impurities and the purification fold of 2.38 was obtained (Table 1). The gel filtration showed two peaks and the second peaks fraction 20 showed HA (Fig. 1). The obtained data in the present study revealed that HA showed a decrease at 40°C (Table 2). On the other hand, a high HA was observed at 30°C. At 10°C the HA showed a least activity. This observation indicates that lectin from cockroach are heat-labile in nature, and shows the highest activity at certain temperatures only. The heat instability is a characteristic of lectins of some other insects, e.g., the orthopteran, Teleogryllus commodus [18], Melanoplus sanguinvpes, [19] and the dipteran, Glossina fuscipes [20], in phasmid Exatosoma tiaratatum [15]. However, in contrast to this, in Leptinotarsa decemlineata [21], and in Locusta migratoria [22], the lectins were reported to be heat resistant. Similarly, we observed HA at different pH ranges of 6–8 pH with the highest activity at pH 7.5 (Table 3). Similar results were observed in Pterockdiella capillacea (pH 7.5) [23], and P. americana (pH 7.5) [13]. Ayaad [24] reported an HA at pH 6.2 for Aedes caspius.

3.2. Divalent Cation Requirement

The study revealed that isolated lectins are calcium-ion-dependent. The cations like Mg2+ or Zn2+ appear to have limited ability to replace Ca2+; however, Ba2+ showed no effect on HA (Table 4). When EDTA was added, complete inhibition of HA was observed. Similar observations were reported on insects having C-type lectin, T. commodus, M. sanguinipes, E. tiaratum, and adult Schistcerca gregari [15,18,19,25]. The mechanism of carbohydrate binding of C-type lectin is suggested by Zelensky and Gready [26]. According to Zelensky and Gready [26], each of the C-type lectins possesses a carbohydrate recognition domain structure with conserved Ca2+ binding sites, and a second site [27]. The lectin of S. gregaria [25] is Ca2+ dependent. However, Yu and Ma [28] suggested that calcium is not required for lectin binding to the sugar, but is necessary to protect it from proteinase digestion. Several workers have reported that the C-type lectin possesses a common carbohydrate recognition domain [29,30] and a second domain [31] that may help in increasing their binding affinity to carbohydrate. The suggested mechanism may involve interactions with the conserved Ca2+ and carbohydrate, which may possibly result in the formation of hydrogen bonding with acid and amide side groups [32]. A similar mechanism is carried out in this study.

Figure 2: Molecular weight of lectin calculated by SDS–PAGE. Lane 1 shows Standard molecular weight markers, and lane 2 shows the purified lectin molecular weight.

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Table 2: The HA of cockroach serum to different temperature.

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Table 3: The HA of cockroach serum to different pH.

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We observed that the HA of P. americana was strong in the presence of calcium, followed by zinc, magnesium, and mercury. The results showed that cockroach HA may be condensed by heavy metal ions, possibly due to protein denaturation. The inhibition of HA of P. americana by Ba2+ was important because it did not show any HA. The plausible reason for this may be that barium ions may be directly bound to the carbohydrate-binding sites, which may seriously affect its conformation modifications resulting in blocked active sites.

Table 4: The HA of cockroach serum to different divalent cations.

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Table 5: The HA of cockroach sera to different blood groups.

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3.3. HA of Whole Serum to Erythrocytes

The HA of serum of P. americana against the tested RBCs in this study is shown in Table 5. The cockroach serum showed a relatively strong HA against goat RBCs, but less detectable activity against human (A, B, and O) and chicken RBCs. The results showed a high specificity of serum lectin to goat RBCs when compared to that of chicken and human.

In this study, the erythrocytes from human, goat, and chicken showed a non-specific HA with cockroach lectin. The lectin agglutinated all these erythrocytes with varying degrees of HA titers. The serum agglutinating activity is rather not highly specific since all the erythrocytes tested showed agglutination and it might be common as naturally occurring agglutinins are specific toward a wide range of vertebrate erythrocytes. The presence of lectin sugar on the surface of erythrocytes may result in an interaction with the lectin resulting in agglutination. The lectin showed a significantly higher HA for goat compared to chicken erythrocytes. The general specificity is influenced by the limited number of contacts with carbohydrates and the depth of the sugar-binding sites [30]. In addition, any modification or substitutions to a binding site can influence the binding specificity [33]. The observed difference in lectin activity with different blood groups may be due to the difference in carbohydrate lectin binding interactions, which can be attributed to differences in carbohydrates present in the cell surface of the different blood groups. The carbohydrates on the erythrocyte cell surface are differently distributed in various animal groups [34]. In the presence of lectin, sugar on the surface of erythrocytes results in an interaction with the lectin, leading to agglutination, or the carbohydrate unit structure and positions may be more specific with higher affinity for binding the lectin. It is possible that a similar mechanism may be responsible for increased goat erythrocyte agglutination. Moreover, lectin characteristics, such as multi-valency, may determine cross-linking interactions in binding recognition spatial distribution of multivalency among lectin structures, which, in turn, produce a higher level of specificity [35,36]. A high HA observed for goat erythrocytes might be due to the occurrence of specific carbohydrate moiety on the surface of goat erythrocytes other than RBCs types [13]. Unfortunately, several erythrocyte types were not examined for the HA activity in the previous reports [37,38].

3.4. Sugar specificity and inhibitions to HA

The inhibition of HA by sugar is shown in Table 6. The carbohydrate specificity of agglutinin is detected in the serum of cockroach and is called as the HA inhibition assay [39,40]. The HA of these lectins was preferentially inhibited by D-galactose, followed by D-lactose, D-glucose, and D-fructose. These results suggest that the carbohydrate-binding and carbohydrate recognition domains prefer α linked D-galactosides rather than free D-galactose. The affinity of the cockroach sera lectins toward α-linked galactosides is reported in L. migratoria [22] and in adult of S. gregaria [25]. The β-D-galactosides, as lectin-specific ligands, are found in insects, coleopteran Allomyrins dichotoma [41]. Barondes [42] also reported β-galactosides, as the lectin specificity in vertebrates, and in orthopterans like T. commodus [18] and the dipteran P. duboscaqi [40] also possess lectins with amino sugars binding affinity. In many species of insects, especially lepidopteran, hemolymph lectins show an affinity for galactose and lactose [43] or to glucosides [21,44]. Some insects also show a binding affinity to mannose, e.g., the dictyopteran, Blaberus discoidalis [45] and lepidopteran, Heliothis virescans pupae [46]. Sugars such as galactose and lactose have been reported as inhibitors of the agglutinating activity of hemolymph of P Americana [37,38]. Volf et al. [40] and McGreal et al. [47] reported that sugars binding specificity is significant for the recognition of carbohydrate moiety on pathogen surface, which are essential and responsible for the ability of the insect as a vector in pathogen transmission. A similar property has been reported in lectins of other insect, B. discoidalis [45], Phlebotomus duboscqu [40], and A. caspius [24].

Table 6: The HA of cockroach serum to different Sugars.

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3.5. SDS-PAGE

SDS-PAGE showed a single band of molecular weight of 26.8 KDa. This molecular weight was near to 30 KDa lectin of P. americana [38], and 20KDa lectin of Saracophaga peregrina [48].


4. CONCLUSION

In conclusion, we say that the lectin purified from cockroach has the highest HA at temperature 30°C and at pH 7.5. The cockroach serum lectin contains a C-type lectin and shows inhibition to D-galactose sugar. Furthermore, the insect’s C-type lectin appears to play an important role in pathogen recognition and cellular interactions. This ability is achieved due to binding with the carbohydrate component of the surface molecular patterns. The SDS-PAGE of lectin showed a molecular weight of 26.8kDa. The isolated lectin was highly specific to goat RBCs than for those of other vertebrates. Accordingly, we can say that the carbohydrate-binding specificity by lectin is necessary for the recognition of the cell surface carbohydrate including those of the invading pathogens.


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1. Dorrah AM, Ayaad TH, Mohamad A, Bassal TTM. Isolation and characterization of multiple lectins from serum of the desert locust, Schistocerca gregaria (Orthopera: Acridiade). J Orthroptera Res 2009;18(1):103-12.https://doi.org/10.1665/034.018.0113

2. FujitaT, Matsushita M, EndoY. The lectin complement pathways its role in innate immunity and evolution. Immunol Rev 2004;198:185- 202.https://doi.org/10.1111/j.0105-2896.2004.0123.x

3. Denis M, ThayappanK, Ramaswamy MS, Arumugan M. Lectin in innate immunity of crustacea. Austin Biol 2016;1:1001-7.

4. Shi XZ, Yu XQ. The extended loop of the C terminal carbohydrate recognition domains of Manduca sexta immulectin-2 is important for ligand binding and functions. Amino Acids 2012;42:2383-91.https://doi.org/10.1007/s00726-011-0980-5

5. Arumugan MG, Sreramulu B, Paulchamy R, Thangevel S, SundaramJ. Purifcation and functional characterization of lectin with phenol oxidase activity from hemolymph of cockroach P ameriana. Arch Insect Biochem Physiol 2017;95:21-39.https://doi.org/10.1002/arch.21390

6. Ayaad TH, Dorrah MA, Mohamed AA, Bassal TTM. Specificity and developmental changes in hemagglutinating activity of serum of the desert locust Schistocerca gregaria (Orthoptera; Acrididae). J Orthoptera Res 2009;18(1):51-6.https://doi.org/10.1665/034.018.0102

7. Richards EH, Ratcliffe NA, Renwrantz I. Isolation and characterization of a serum lectin from the giant stick insect, Exatosoma tiaratatum. Insect Biochem 1988;18:691-700.https://doi.org/10.1016/0020-1790(88)90078-9

8. Lowry OH, Rosebrough NL, Forr Al, Randell RJ. Protein measurement with Folin Phenol reagent. J Biol Chem 1951;192:265-75.

9. Laemmli UK. Cleavage of structural protein during the assembly of head of bacteriophage T4. Nature 1970;227:680-5.https://doi.org/10.1038/227680a0

10. Hapner KD, Jermyn MA. Hemagglutinin activity in the hemolymph of Teleogryllus comodus. Insect Biochem 1981;11:287-95.https://doi.org/10.1016/0020-1790(81)90006-8

11. Stebbins MR, Hapner KD. Preparation and properties of hemagglutinin from hemolymph of Acrididae (Grasshopper). Insect Bichem 1985;15:451-62.https://doi.org/10.1016/0020-1790(85)90057-5

12. Ingram GA, Molyneux DH. Lectins in the hemolymph of Glossina fuscipes fuscipes: isolation, partial characterization, selected physico-chemical properties and carbohydrate binding specificities. Insect Biochem 1990;20:13-27.https://doi.org/10.1016/0020-1790(90)90017-O

13. Minnick ME, Rupp RA, Spence KD. A bacteria induced lectin which triggered hemocyte coagulation in Manduca sexta. Biochem Biophysical Res Commun 1986;137:729-36.https://doi.org/10.1016/0006-291X(86)91139-3

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Onaldo Guedes Rodrigues, Brunna Rodrigues Muniz Falcão, Beatriz Correa Barbosa, Andreia Vieira Pereira, Vitoria Viviane Ferreira de Aquino

Characterization of indole-3-acetic acid in cambial zone tissue by ultra-performance liquid chromatography-tandem mass spectrometry

Renan Marcel Bonilha Dezena, Renata Mauri, Fabio Alessandro Proença Barros, Paulo César Pires Rosa

Polyphenols and triterpenes in leaves and extracts from three Nicotiana species

Venelina Popova, Tanya Ivanova, Albena Stoyanova, Violeta Nikolova, Tsveta Hristeva, Margarita Docheva, Nikolay Nikolov, Ivan Iliev

Biodiversity of psychrotrophic microbes and their biotechnological applications

Ajar Nath Yadav, Neelam Yadav, Shashwati Ghosh Sachan, Anil Kumar Saxena

Comparative evaluation of air pollution tolerance of plants from polluted and non-polluted regions of Bengaluru

BT Manjunath, Jayaram Reddy

Low dose and subchronic Aroclor 1254 induced histopathological changes in mice kidney

Jalpa Raja, Shweta Pathak, Rahul Kundu

Phytochemical analysis, antimicrobial and antioxidant activities of Aidia borneensis leaf extracts

Zulhamizan Awang-Jamil, Aida Maryam Basri, Norhayati Ahmad, Hussein Taha

Influence of salinity stress on the uptake of magnesium, phosphorus, and yield of salt susceptible and tolerant sorghum cultivars (Sorghum bicolor L.)

Abida Kausar, Munazza Gull

Weeds in Gwalior and nearby regions and their medicinal properties: A case study

Sapna Kondalkar, Avinash Kondalkar, Vijay Kumar, Anupam K Mangal, Om Prakash

Comparative chemical and biological investigations of three Saudi Astragalus species

Mohamed A. Ashour

Phytochemical analysis and antioxidant potential of Ocimum gratissimum Linn (Lamiaceae) commonly consumed in the Republic of Benin

Hinnoutondji Wilfrid Kpètèhoto, Abdou Madjid Olatoundé Amoussa, Roch Christian Johnson, Eustache Enock Meinsan Houéto , Franck Maurille Zinsou Mignanwandé, Hounnankpon Yédomonhan, Frédéric Loko, Honoré Bankolé, Latifou Lagnika

Determination of phenolic content and antioxidant capacity of Launaea resedifolia from Algerian Sahara

Amina Bouguerra, Mohamed Hadjadj, Mesaouda Dekmouche, Zhour Rahmani, Houssine Dendougui

In vitro anti-amylase activity of some Indian dietary spices

Bhosale Hemlata, Gawali Pornima, Kadam Tukaram, Baisthakur Pankaj

Mineralization and mobilization of biosolids phosphorus in soil: A concise review

Abubakar Musa Ahmad, Adamu Yunusa Ugya, Habib Adamu Isah, Tijjani Sabiu Imam

Screening and characterization of siderophore producing endophytic bacteria from Cicer arietinum and Pisum sativum plants

Rajat Maheshwari, Namita Bhutani, Pooja Suneja

Vibriocidal activity of leaf and rhizome extracts of Maranta arundinacea L.

Priyadarshani Samal, Priyaranjan Tripathy, Ritarani Das, Santi Lata Sahoo, Chinmay Pradhan, Bijaya Kumar Padhi, Jyoti Ranjan Rout

Root-fungal associations in plants from home gardens of Tripura, Northeast India

Kripamoy Chakraborty, Atithi Debnath, Aparajita Roy Das, Ajay Krishna Saha, Panna Das

Identification of TNF-Þ-308G/A (rsl800629) polymorphism in Bangladeshi population related to type-2 diabetes

Dipa Roy, Md Sk Shahid Ullah, Bristy Basak, Md. Mahmudul Hasan, Maisha Tanzim, Ariful Haque

Genetics characterization of fresh water clam Spathopsis rubens using ISSR-PCR markers

Gihan Mahmoud Elkhodary, Amal Zaki Ghoneim

Genome-wide analysis of various paralogs of aminopeptidase N (APN) gene in Anopheles gambiae (Diptera: Culicidae)

Renu Jakhar, Ritu Gill, Surender Kumar Gakhar

Factors affecting the chitinase activity of Trichoderma asperellum isolated from agriculture field soils

Ndiogou Gueye, G Kranthi Kumar, Malick Ndiaye, S Y Dienaba Sall, Mame Arama Fall Ndiaye, Tahir A Diop, M Raghu Ram

Kinetic and thermodynamic parameters of four acid phosphatases from edible seeds of oleaginous cucurbit Lagenaria siceraria (Molina) Standl.

Djary Michel Koffi, Jean Bedel Fagbohoun

Nyctanthes arbor-tristis: Comprehensive review on its pharmacological, antioxidant, and anticancer activities

Smita Parekh, Anjali Soni

Bioaccumulation of heavy metal lead (Pb) in different tissues of brackish water fish Mugil cephalus (Linnaeus, 1758)

Vardi Venkateswarlu, Chenji Venkatrayulu

Purification and characterization of extracellular alkaline protease from Streptomyces sp. LCJ12A isolated from Pichavaram mangroves

M. Parthasarathy, J. Joel Gnanadoss

Improvement of germination rate and in vitro multiplication of Lavandula angustifolia

Chaimae Slimani, Hakima Sqalli, Chaimae Rais, Squalli Wafae, Abderrahim Lazraq, Lahsen El Ghadraoui, Saadia Belmalha, Ghizlane Echchgadda

A simple and efficient method for removal of phenolic contaminants in wastewater using polyacrylamide entrapped mushroom tyrosinase

Anupama Sharma Avasthi, Sabari Ghosal

In vitro anthelmintic activity of methanol extracts and fractions of two amphilophium species against Eisenia Fetida

Diana Bazana, Ever Lopezb, Andrea Caceresa, Rosa Degenb, Nelson Alvarengaa

Screening and evaluation of PGPR strains having multiple PGP traits from hilly terrain

Teg Bahadur Singh, Vikram Sahai, Akbar Ali, Mrinalini Prasad, Arti Yadav, Preksha Shrivastav, Deepika Goyal, Prem Kumar Dantu

The phytochemical and chemotaxonomic study of Salvia spp. growing in Ukraine

Oleh Koshovyi, Ain Raal, Alla Kovaleva, Mykhailo Myha, Tetyana Ilina, Natalia Borodina, Andrey Komissarenko

Optimization of physical parameters for the growth and lipid production in Nannochloropsis gaditana (Lubian, 1982)

Shyni MarKose, Ajan Chellappan, Praba Thangamani, Subilal George, Selvaraj Thangaswamy, Citarasu Thavasimuthu, Michaelbabu Mariavincent

Efficacy of antibiotic sensitivity and antimicrobial activity of Streptomyces cinereoruber RSA-14 isolated from rhizosphere soil of Alternanthera sessilis (L.) R. Br. ex DC

Sreenivasa Nayaka, Halaswamy Hiremath, Bidhayak Chakraborty, Pallavi Sathyanarayan Swamy, Dhanyakumara Shivapoojar Basavarajappa, Shashiraj Kareyallappa Nagaraja, Muthuraj Rudrappa, Meghashyama Prabhakara Bhat, Dattatraya Airodagi, Meenakshi Shivanandagouda Murigennavar

Taxonomic validation of Areolate grouper, Epinephelus areolatus (Perciformes: Serranidae) along the Nizampatnam coast, India

Chatla Darwin, Padmavathi Pamulapati, Gatreddi Srinu

Purification and characterization of an iron-activated alkaline phosphatase produced by Rhizopus microsporus var. microsporus under submerged fermentation using rye flour

Pedro Henrique de Oliveira Ornela, Luis Henrique Souza Guimarães

Comparison of diagnostic performance between serological tests (Scrub IgM-ELISA) and Scrub-PCR for scrub typhus fever in an Indian tertiary care teaching hospital

Anil Gatika, Subrat Kumar Swain, Jatindra Nath Mohanty, Rachita Sarangi

Determination of the activity and kinetic parameters of polyphenol oxidase enzyme in crude extracts of some Jordanian plants

Omar Mohammad Atrooz, Nooh Khair AlKhamaisa, Ibrahim M. AlRawashdeh

Physicochemical, phytochemical, and GC–MS analysis of leaf and fruit of Pouteria campechiana (Kunth) Baehni

Akshata Pai, Chandrakala Shenoy

In vitro antioxidant activity of Lactobacillus plantarum against hydrogen peroxide-induced neuronal damage on PC12 cells

Shani Kunjamma John, Vani Chandrapragasam

Identification and in-silico characterization of differentially expressed salt-induced proteins in the leaves of mangrove grass Myriostachya wightiana

Mathikani Kiran Kumar, B V Sandeep, Pola Sudhakar

Distribution and species listing of wild macrofungi in Sitio Canding, Barangay Maasin, San Clemente, Tarlac Province, Philippines

Rich Milton Dulay, Jose Santos Carandang VI, Sofronio Kalaw, Renato Reyes

Development and validation of multiplex polymerase chain reaction assay for concomitant detection of genus Staphylococcus and clinically relevant methicillin resistance determinants

Nimita Venugopal, Feroze Ganaie, Susweta Mitra, Rituparna Tewari, Tushar K. Dey, Rakshith Ojha, Rajeswari Shome, Bibek R. Shome

Distribution and diversity of endophytic fungi associated with three medicinal tree species from Eturnagaram Wildlife Sanctuary, TS, India

Bhavani Vemireddy, Abhinesh Madasi, Aruna Ajmeera, Krishna Reddy Vanteru

Interactive potential of Pseudomonas species with plants

Suhana Shaikh,, Nutan Yadav, Anoop R. Markande,

Screening of in vitro sun protection factor of some medicinal plant extracts by ultraviolet spectroscopy method

Manisha Pralhad Sutar, Sanjay Ravindra Chaudhari

Molecular identification and optimization of cultural conditions for mycelial biomass production of wild strain of Chlorophyllum molybdites (G.Mey) Massee from the Philippines

Benjie L. Garcia, Jerwin R. Undan, Rich Milton R. Dulay, Sofronio P. Kalaw, Renato G. Reyes

Effects of culture media and physical factors on the mycelial growth of the three wild strains of Volvariella volvacea from Ecuador

Marcelino D. Abon, Rich Milton R. Dulay, Sofronio P. Kalaw, Maria Eugenia Romero-Roman, Lenin Pedro Arana-Vera, Walter Oswaldo Reyes-Borja, Renato G. Reyes

Identification of highest L-Methioninase enzyme producers among soil microbial isolates, with potential antioxidant and anticancer properties

D. Kavya, Varalakshmi Kilingar Nadumane

African plum (Dacryodes edulis [G. Don] H.J. Lam) fruit development indexes clearly defined and phenophases correlated with temperature in the tropics

Salamatou Mpemboura Nsangou, Libert Brice Tonfack, Didier Mbeguie-A-Mbeguie, Carine Temegne Nono, Godswill Ntsomboh-Ntsefong, Emmanuel Youmbi

Comparative morphological, histological, and RAPD analysis of Columba livia domestica strains

Dalia A. Sabry, Yosra A. Fouda

An effective and eco-friendly technique for control of post-harvest fungal pathogens of orange (Citrus sinensis) isolated from the distribution chain of Delhi NCR

Gayatri Krishna, Geethu Gopinath, Anupama Sharma Avasthi

Amylase production by Aspergillus niger in submerged cultivation using cassava

Muralikandhan Kamaraj, Dhanasekaran Subramaniam

Evaluation of thermogravimetric analysis as a rapid tool for the detection of rhizobacteria biostimulants used in precision agriculture

Eugene Carmichael, Juluri R. Rao

Multidrug-resistant Candida auris: A global challenge

Hardeep Kaur, Khushbu Wadhwa, Kusum Jain, Anamika Yadav

Evolution of matK Gene among the Elite Tea Clones (Camellia sinensis) Revealed by Nucleotide Substitution within the Consensus Region

Reha Labar, Pallab Kar, Prosenjit Biswas, Arnab Sen, Malay Bhattacharya

In-vitro activity of ethanolic extract of Lentinus strigosus mycelia in N2 wild strain Caenorhabditis elegans – An animal model for obesity and its chemical composition

Benditha D. Babac, Rich Milton R. Dulay, Reign Arwen S. Calpito, Maricris A. Domingo, Mary Grace M. Macamos, Andrea R. Mangabat, Neil Zhyra L. Zoberiaga

The comparative antimicrobial and anticancer of chemical extract from in vitro and in vivo Peperomia pellucida plantlet

Lydia Teoh, Nareshwaran Gnanasegaran, Ahmad Faris Mohd Adnan, Rosna Mat Taha

Floral induction pathways: Decision making and determination in plants to flower-A comprehensive review

Latif Ahmad Peer, Mohd Yaqub Bhat, Nusrat Ahmad, Bilal Ahmad Mir

Plasticity of tandem repeats in expressed sequence tags of angiospermic and non-angiospermic species: Insight into cladistic, phenetic, and elementary explorations

Shamshad Ul Haq, Prerna Dhingra, Meenakshi Sharma, S. L. Kothari, Sumita Kachhwaha

Effects of light on cell growth, chlorophyll, and carotenoid contents of Chlorella sorokiniana and Ankistrodesmus falcatus in poultry dropping medium

Jane Chizie Ogbonna, Nkechinyere Onyekwere Nweze, Christiana Nwakego Ogbonna

Some roadside medicinal weeds as bio-indicator of air pollution in Kolkata

Pranabesh Ghosh, Sirshendu Chatterjee, Suradipa Choudhury, Tanusree Sarkar, Ahana Sarkar, Susmita Poddar

Microbial biotechnology for bio-prospecting of microbial bioactive compounds and secondary metabolites

Ajar Nath Yadav

Evaluation of immunomodulatory activity of Immusante® in zebrafish

Anna Venkata Agraz, Mohamed Rafiq, Bhavimani Guru, Madan MN, Suryakanth Dattatreya Anturlikar, Azeemuddin MM, S. N. Manjula

Bioprospecting of endophytic bacteria from the Indian Himalayas and their role in plant growth promotion of maize (Zea mays L.)

Kusam Lata Rana, Divjot Kour, Tanvir Kaur, Rubee Devi, Ashok Yadav, Ajar Nath Yadav

Effect of perforation size and substrate bag fruiting position on the morphology of fruiting bodies and clusters in Pleurotus ostreatus (Jacq.) P. Kumm

Iryna Bandura, Omoanghe S. Isikhuemhen, Alina Kulik, Marina Serdiuk, Olena Sucharenko, Valentina Jukova, Viktoriia Koliadenko, Nona Gaprindashvili

Optimization of culture conditions for mycelial growth and fruiting body production of naturally-occurring Philippine mushroom Lentinus swartzii Berk

Rich Milton R. Dulay, Esperanza C. Cabrera, Sofronio P. Kalaw, Renato G. Reyes

Modulation of photosynthesis, nitrogen fixing ability, and yield attributes of Chickpea (Cicer arietinum L.) to interactive effect NPK fertilizers and municipal wastewater irrigation

Hamid Iqbal Tak

Modification of the time of incubation in colorimetric method for accurate determination of the total antioxidants capacity using 2,2-diphenyl-1-picrylhydrazyl stable free radical

Abhipsa Bal, Samar Gourav Pati, Falguni Panda, Biswaranjan Paital

Differences in antibiotic resistance profiles of methicillin-susceptible and –resistant Staphylococcus aureus isolated from the teaching hospital in Kuala Lumpur, Malaysia

Asif Sukri, Muhammad Nur Farhan Saat, Nor Afnizan Mohd Yusof, Noraziah Mohamad Zin, Abdul Rahim Abdul Rachman

Role of lacto-fermentation in reduction of antinutrients in plant-based foods

Mehak Manzoor, Deepti Singh, Gajender Kumar Aseri, Jagdip Singh Sohal, Shilpa Vij, Deepansh Sharma

Antimicrobial activity of the lichens Parmotrema andium and Dirinaria applanata

Bijayananda Sahoo, Satyabrata Dash, Sabyasachy Parida, Jayanta Kumar Sahu, Biswajit Rath

Micro-morphological and anatomical response of groundnut (Arachis hypogaea L.) cultivars to ground-level ozone

Indra Jeet Chaudhary, Dheeraj Rathore

Biodiversity and bioprospecting of extremophilic microbiomes for agro-environmental sustainability

Ajar Nath Yadav

Acute toxic effects of chlorpyrifos and indoxacarb on dorsal pars medialis of telencephalon and swimming behavior of a catfish, Heteropneustes fossilis

Ajeet Kr. Mishra, Anita Gopesh, K. P. Singh

Role of medicinal plants in the treatment of eumycetoma: A review

Shashank M. Patil, S. Jagadeep Chandra, M. K. Jayanthi, Prithvi S. Shirahatti, Ramith Ramu

Analysis of the chemical composition (GC–MS) of Lantana camara (Verbenaceae) essential oil and its insecticidal effect on the post-embryonic development of Chrysomya megacephala (Fabricius, 1794) (Diptera: Calliphoridae)

Rahul Maddheshiya, Deep Mala Sahu, Krishna Pal Singh

Identification and bioactivities of endophytic fungi from Lagenandra toxicaria Dalz. and Kaempferia rotunda L.

Praveen Krishnakumar, Mable Varghese, Maria Grace Joe, Asha Rajagopal, Leyon Varghese

The effect of Staphylococcus epidermidis cells on Pseudomonas aeruginosa-associated virulence factors

Suhaga Dohare, Devendra Singh, Deepmala Sharma, Vishnu Agarwal

Beneficial effects of soaking and germination on nutritional quality and bioactive compounds of biofortified wheat derivatives

Meena Verma, Vinod Kumar, Imran Sheikh, Punesh Sangwan, Roop Singh Bora, Ajar Nath Yadav, Harcharan Singh Dhaliwal

Characterization of Calotropis procera root peroxidase and its potential to mediate remediation of phenolic pollutant from petroleum refinery effluent

Enoch Banbilbwa Joel, Simon Gabriel Mafulul, Ezra Adams Jeremiah, Adepeju Aberuagba, Raphael Idowu Adeoye, Lazarus Joseph Goje, Adedoyin Igunnu, Sylvia Omonirume Malomo

Applications of bacterial endophytes and their advanced identification methodologies

R. Renugadevi, M. P. Ayyappadas, V. Subha Priya, M. Flory Shobana, K. Vivekanandhan

Morphological status of multipolar neurons in the lateral cortical area of the telencephalon of a catfish, Clarias batrachus: A Golgi study

Banalata Mohanty, Anil Kumar Ojha

Prevalence of malaria and typhoid fever and coinfection in all febrile patients attending at tertiary care hospital in Vadodara

Sangita Devubhai Vasava, Sucheta Jitendra Lakhani, Jitendra Devjibhai Lakhani

Doehlert Matrix-assisted optimization of Salmonella typhi Vi polysaccharide purification parameters

Garisala Mallikarjunara Rao, Sathish Thadikamala, Maram Chinna Eswaraiah

Purification, characterization of α-galactosidase from a novel Bacillus megaterium VHM1, and its applications in the food industry

Aravind Gouda G. Patil, Naganagouda V. Kote, A. C. Manjula, T. Vishwanatha

Diversity and antimicrobial potential of endophytic fungi from aromatic plants of Bhadra Wildlife Sanctuary, Western Ghats, Karnataka

Rajeshwari Jagadish, Srinivas Chowdappa

Molecular docking of active compounds from Phoenix pusilla root extract against antidiabetic and anti-inflammatory drug targets

Vijayabharathi Saravanan, Anuradha Venkatraman, Habeeb Shaik Mohideen

Rice crop loss due to major pathogens and the potential of endophytic microbes for their control and management

Shubhransu Nayak, Soma Samanta, Chandan Sengupta, Soumya Sephalika Swain

DNA barcode of matK combined with ITS effectively distinguishes the medicinal plant Stephania brachyandra Diels collected in Laocai, Vietnam

Nhan Thi Thanh Pham, Dung Phuong Le, Khanh Thi Ngoc Pham,, Xaykham Thipphavong,, Mau Hoang Chu

Molecular identification of endophytic fungi associated with Coleus forskohlii (Willd.) Briq.

Grace Leena Crasta,, Koteshwar Anandrao Raveesha,

Augmentative role of Piriformospora indica fungus and plant growth promoting bacteria in mitigating salinity stress in Trigonella foenum-graecum

Sanskriti Bisht, Shatrupa Singh, Madhulika Singh,, Jai Gopal Sharma

Phytomicrobiomes for agro-environmental sustainability

Ajar Nath Yadav

Analytical study on hexavalent chromium accumulation in plant parts of Pongamia pinnata (L.) Pierre and remediation of contaminated soil

Pratyush Kumar Das, Bidyut Prava Das, Patitapaban Dash

Bacterial endophytes from halophyte black saxaul (Haloxylon aphyllum Minkw.) and their plant growth-promoting properties

Vyacheslav Shurigin,, Begali Alikulov, Kakhramon Davranov, Zafar Ismailov

The ITS2 DNA sequence analysis in six species of barbin fishes with phylogenetic insights

Sandip Choudhury, Briyanka Kashyap, Karabi Dutta

Mushroom fruiting body yield and morphological characteristics from different strains of Pleurotus eryngii

Iryna Bandura, Omoanghe S. Isikhuemhen, Alina Kulik, Nina Bisko, Maryna Serduik, Volodymyr Khareba, Olena Khareba, Iryna Ivanova, Olesya Priss, Oleksandr Tsyz, Serhii Makohon, Serhii Chausov

Partial identification of a peptide from Lacticaseibacillus casei using MALDI TOF and it’s cytotoxic activity against MDA-MB-231 cell line

Jannatul Firdous Siddique, Mohanasrinivasan Vaithilingam

Synergistic effect of Hydnophytum formicarum tuber and Vatica diospyroides Symington cotyledon extracts with ampicillin on pathogenic bacteria

Kanokrat Keawchai, Parinuch Chumkaew, Patima Permpoonpattana, Theera Srisawat

Allelopathic potential of Annona muricata (L.) on physiological and biochemical changes of Vigna radiata (L.) and Eleusine coracana (L.) Gaertn.

Edwina Kannan, Leela Palayian

Phytochemical, pharmacological, and pharmacognostic overview of Merremia tridentata (L.) Hallier

Paras Sharma, Gaurav Gupta

Genetic polymorphism of soybean genotypes with contrasting levels of phosphatidylcholine, protein, and lipoxygenase-2

Aseem Kumar Anshu,, Trupti Tayalkar, Anita Rani, Vineet Kumar, Hamendra Singh Parmar

Suppression of the RAGE gene expression in RAW 264.7 murine leukemia cell line by ethyl acetate extract of Mikania micrantha (L.) Kunth.

Alex Zohmachhuana, Malsawmdawngliana Tlaisun, Vabeiryureilai Mathipi, Lalrinzuali Khawlhring, Joyce Sudandara Priya

Dietary product based on sea urchin caviar and Sardinops melanostictus fat

Mouhamad Alrajab, Lidiya Vasilevna Shulgina

Characterization and comparative assessment of bactericidal activity of carbon nanodots (CDs) and nanoparticles (CNPs) prepared from soot's of clarified butter and mustard oil, respectively

Vikas Pahal, Pankaj Kumar, Rahul Kumar, Parveen Kumar, Vinod Kumar

Inducing the growth and flowering of caraway (Carum carvi L.) plant

Hanan M. H. Ali, Dalia Abdel Halim M. Sallam

Isolation and identification of potent tributyltin chloride utilizing Pseudomonas sp. strain VBAK from Vishakhapatnam shipping harbor sediments

Battu Anand Kumar, Triveni Gummadi, Bhargava Ram Gundluri, Chanda Vikrant Berde, Veera Bramhachari Pallaval

Characterization and expression of phenylalanine hydroxylase in rabbits under different feeding conditions of faba bean (Vicia faba L.)

Hanan I. Sayed Ahmed, Ghada Shebl, Mohamed Hamza, Asraf Haider

Quantification of phytochemicals and in vitro antioxidant activities from various parts of Euphorbia neriifolia Linn.

Priya Chaudhary, Pracheta Janmeda

Effect of extraction methods and temperature preservation on total anthocyanins compounds of Peristrophe bivalvis L. Merr leaf

Nguyen Minh Thuy, Dao Huynh Ngoc Han, Vo Quang Minh, Ngo Van Tai

Molecular detection of Haemophilus influenzae isolated from eye swabs of patients with conjunctivitis in Hilla Province, Iraq

Jundi Alak Mahdi Al-Buhilal, Abdulkadir Kareem Rhumaid, Alyaa Mohammed H. Al-Tabtabai, Niran Kadhim F. AL-Rubaey

The propensity of selected Indian plant extracts for polyphenolics, antioxidant, and inhibition of Pseudomonas aeruginosa biofilms through type-3 secretion system

M. G. Avinash, Farhan Zameer,, Shubha Gopal

Purification and characterization of a polygalacturonase from the xylophagous insect Oncideres albomarginata chamela (Coleoptera: Cerambycidae)

Alicia Lara-Márquez, Nayeli Soria-Calderón, Maria Guadalupe Villa-Rivera, Everardo López-Romero, Nancy Calderón-Cortés

Investigating Musa paradisiaca (Var. Nanjangud rasa bale) pseudostem in preventing hyperglycemia along with improvement of diabetic complications

Ramith Ramu,, Prithvi S Shirahatti, Deepika TH, Shrisha Naik Bajpe, Navya Sreepathi,, Shashank M Patil, Nagendra Prasad MN

Isolation and partial characterization of amylase produced by fungal isolates from the agro-industrial waste source

Soumya Nandi, Twinkle Bose, Susmita Mahato, Somashree Chatterjee, Annalakshmi Chatterjee

Virulence of native entomopathogenic nematodes against major lepidopteran insect species of tomato (Solanum lycopersicum L.)

Neelam Thakur, Preety Tomar, Simranjeet Kaur, Poonam Kumari

Molecular diversity and phylogenetic analysis of exopolymer producing fungal strains isolated from Krishna river mangrove sediments

Annavarapu Mohana Ventaka Naga Prathyusha, Venkateswarulu Nagam, Chanda Vikrant Berde, Siva Saikiran Badithala, Muni Ramanna Gari Subhosh Chandra, Thummala Chandra Sekhar, Veera Bramhachari Pallaval

Microbes for Agricultural and Environmental Sustainability

Ajar Nath Yadav, Divjot Kour, Ahmed M. Abdel-Azeem, Murat Dikilitas, Abd El-Latif Hesham, Amrik Singh Ahluwalia

Characterization of tannery effluents by analyzing the recalcitrant organic pollutants and phytotoxicity assay

Sandeep Kumar, Ashutosh Yadav, Annapurna Maurya,,Shalini G. Pratap, Pramod Kumar Singh, Abhay Raj,

In silico studies of viral protein inhibitors of Marburg virus using phytochemicals from Andrographis paniculata

Ragupathy Hariprasath, Chandrasekar Akashpriya, Vasantha Veerappa Lakshmaiah, Nagella Praveen

Cowpea (Vigna unguiculata L. Walp.): A choice crop for sustainability during the climate change periods

Tewodros Ayalew, Tarekegn Yoseph

Woodfordia fruticosa (Linn.) Kurz’s fungal endophyte Mucor souzae’s secondary metabolites, kaempferol and quercetin, bestow biological activities

Kavyashree Doreswamy, Priyanka Shenoy, Sneha Bhaskar, Ramachandra K. Kini, Shailasree Sekhar

Characterization of the crude extract of Portulaca oleracea and the determination of the polyphenol oxidase kinetics in the presence of Cu and Zn

Omar Mohammad Atrooz, Shada Zaher Al-Maitah

Assessing DNA barcodes species discriminating ability and phylogenetic relation within Embelia species

Shrisha Naik Bajpe,, K. M. Marulasiddaswamy, Ramith Ramu, Abhijeeth S. Badiger, Maruthi Katenahally Rudrappa, Ramachandra Kukkundoor Kini

Molecular characterization and antibacterial properties of endophytic fungi Lasidiplodia theobromae in Lobelia nicotianifolia Roth ex Schult. of central Western Ghats of Karnataka, India

Krishnappa Vinu, Maddappa Krishnappa, Venkatarangaiah Krishna

Seasonal effect on the diversity of soil fungi and screening for arsenic tolerance and their remediation

Dheeraj Pandey, Harbans Kaur Kehri, Ifra Zoomi, Shweta Chaturvedi, Kanhaiya Lal Chaudhary

Enhancing antibacterial properties of bacteriocins using combination therapy

Poonam Sharma, Meena Yadav

Phenotypic variation and genetic divergence studies in Cameroonian potato (Solanum tuberosum L.) genotypes

Mariette Anoumaa, Eric Bertrand Kouam, Gabriel Kanmegne, Yanick Borel Kamga, Hervé Djomo Sime, Lisette Laure Chimi Nkombo, Théophile Fonkou

Phosphate-Solubilizing Microorganisms for Agricultural Sustainability

Ajar Nath Yadav

Additive and antagonistic effects of selected polyphenols on biochemical indices of isoproterenol-induced toxicity in Wistar rats

Grace Jadesola Oyinbogbola, Mary Tolulope Olaleye, Aanuoluwapo Ruth Adetuyi, Ibrahim Olabayode Saliu, Afolabi Clement Akinmoladun

Identification, production, and purification of a novel lipase from Bacillus safensis

Krishna Patel, Samir Parikh

Evaluation of plant growth-promoting activities of endophytic bacteria of Musa acuminata and their characterization

Shilpi Singh, Kamlesh Choure, Piyush Kant Rai, Sourabh Singh Gour, Vivek Kumar Agnihotri

Evolution of machine learning in biosciences: A bibliometric network analysis

Akkinepally Vanaja,, Venkata Rajesh Yella

Isolation and characterization of starch degrading bacteria from disparate soil samples

Vidhyutha Srivathsan, Mahima Bhandari, Priya Swaminathan

Antimicrobial, anticancer, and antioxidant potential of two dominant macro-lichen Dirinaria aegialita and Parmotrema praesorediosum collected from Similipal Biosphere Reserve of Odisha, India

Srimay Pradhan, Dalip Kumar Upreti, Rajesh Kumar Meher, Kunja Bihari Satapathy

An epidemiological outbreak of scrub typhus caused by Orientia tsutsugamushi – A comprehensive review

Subhasmita Panda, Subrat Kumar Swain, Rachita Sarangi

Investigation on the antifungal activity of Aspergillus giganteus in different culture conditions

S. Karthiga, R. Ramya, K. Ramya, S. Jothinayaki, D. Kavitha

Accumulation of cadmium in maize roots inoculated with root organ culture of Rhizophagus irregularis improving cadmium tolerance through activation of antioxidative defense enzymes

Rajamony VinuRadha, Karunanandham Kumutha, Asish Kanagaraj Binodh

Comprehensive reviews on phenolic compounds from Phaeophyceae as potential therapeutic agent

V. Maheswari, P. Azhagu Saravana Babu

Endophytic bacterial metagenomics and phosphate solubilization activities in an endemic legume Humboldtia brunonis Wall.

Ganesh V. Shendye, N. Thamizhseran

Identification of phytocompounds from Paris polyphylla Smith as potential inhibitors against two breast cancer receptors (ERÞ and EGFR tyrosine kinase) through chromatographic and In silico approaches

Debmalya Das Gupta, Saurov Mahanta, Sasti Gopal Das, Sanjib Kumar Das, Dipayan Paul, Hui Tag, Pallabi Kalita Hui

In silico modeling, docking of ThPON1-like protein, and in vitro validation of pesticide tolerance in Trichoderma harzianum

Archana Kumari, Krishna Sundari Sattiraju

Comparative analysis of Physico-chemical properties and fatty acid composition of linseed (Linum usitatissimum L.) oils of Indian accessions

Nagabhushanam Beema, Nagaraju Mukkamula, Srinivas Mothuku, Ravinder Thumu, Thirupathi Azmeera, Kiran Kumar Biman

In vitro evaluation of the antibacterial potential of flavonoid glycosides from Glinus oppositifolius (L.) Aug. DC.

K. Suresh Kannan, D. Kandavel, P. Rajalakshmi, P. Maheswari

Elevating algal biomass generation toward sustainable utilization for high value added biomolecules generations

Kamalendu De, Shrestha Debnath, Dipankar Ghosh

Antimycobacterial and antibiofilm activity of garlic essential oil using vapor phase techniques

Ashirbad Sarangi, Bhabani Shankar Das, Sunil Swick Rout, Ambika Sahoo, Sidhartha Giri, Debapriya Bhattacharya

Development of Ready-to-Eat functional food prepared from seeded banana Bhimkol (Musa balbisiana Colla) and Black rice (Oryza sativa L.)

Himakshi Baishya, Ranjita Goswami, Lakhya Jyoti Gogoi

Multitargeted molecular docking study of phytochemicals on hepatocellular carcinoma

Vikas Jha, Anjali Bhosale, Prakruti Kapadia, Agraj Bhargava, Arpita Marick, Zahra Charania, Omkar Parulekar, Mafiz Shaikh, Bhakti Madaye

Growing rice (Oryza sativa L.) in the northern Gangetic plains of India. How suitable are they? A GIS-based multi-criteria analysis

Sambit Shome, Hina Upadhyay

Degradation and detoxification of monocrotophos using bacterial consortium

Priyanka Harenkumar Jokhakar, Pravin R Dudhagara

Occurrence of two endophytic associative nitrogen-fixing Caulobacter spp., from three non-nodulating endemic legumes based on nifH gene analysis

N. Thamizhseran, Ganesh V. Shendye

A comprehensive review on global research trends on Aerides genus with reference to Aerides odorata species

Vivek Kumar Paraste, Surendra Sarsaiya,, Umesh C. Mishra, Pragya Sourabh

Records of wild mushrooms in the Philippines: A review

Rich Milton R. Dulay, Joshua N. Batangan, Sofronio P. Kalaw, Angeles M. De Leon, Esperanza C. Cabrera, Kenichiro Kimura, Fumio Eguchi, Renato G. Reyes

Transaminases activity in the hemolymph: Biomarkers determining the thermal stress in the new bivoltine lines of Bombyx mori.

J. Prashanth, H. B. Manjunatha

Impact of different levels of iron fertilizer on growth and yield physiology of Kodo millet under rainfed conditions – An overview

R. Samundeswari, N. Jeyapandiyan, M. Anitha, J. Patricia Kalaiarasi, R. Susan Poonguzhali, C. Jayapradha, S. Rathikannu, K. Udhaya Kumar

Field treatment of three wheat varieties with Trichoderma harzianum bioagent to control Anguina tritici

Nawres Abdulelah Sadeq Alkuwaiti, Ammar Amjad Aish, Saad Tareq Abdulmalak, Tariq A. Kareem, Mohammed Mahmood Sulaiman

Elemental, nutritional, and phytochemical profiling and antioxidant activity of Cordia obliqua Willd. (Clammy Cherry): An important underutilized forest tree of East India

Mamta Naik#,, Shashikanta Behera#,,, Sadhni Induar, Swaraj K. Babu, Pradeep K. Naik

Endophytic nitrogen-fixing bacteria: Untapped treasurer for agricultural sustainability

Kusam Lata Rana, Divjot Kour, Tanvir Kaur, Rajeshwari Negi, Rubee Devi, Neelam Yadav, Pankaj Kumar Rai, Sangram Singh, Ashutosh Kumar Rai, Ashok Yadav, R. Z. Sayyed, Ajar Nath Yadav

Rhizospheres of Rubus ellipticus and Ageratina riparia from Meghalaya exhibit Actinomycetota that promote plant growth

Debulman Syiemiong, Dhruva Kumar Jha, Samrat Adhikari, Dapkupar Mylliemngap, Richborn Kharbuki, Dominic Lyngdoh, Joel Paul Warlarpih, Neha Paul, Kevin Matthew Lamare, Chalcedony Wahlang, Rangehbok Lyngkhoi

Effect of salicylic acid, jasmonic acid, and a combination of both on andrographolide production in cell suspension cultures of Andrographis paniculata (Burm.f.) Nees

Nihal Ahmed, N. Praveen

Antimicrobial peptide coding gene of thermophilic bacteria isolated from crater hot spring in mountains around West Java

Emma Rachmawati, Sinta Asarina, Gabriel Bagus Kennardi, Ratu Safitri, Toto Subroto, Ani Melani Maskoen

Endophytic Fungi as Emerging Bioresources for Bioactive Compounds for Sustainable Development

Divjot Kour, Neelam Yadav, Ajar Nath Yadav

Pseudomonas aeruginosa biofilm and their molecular escape strategies

M. G. Avinash, S. Aishwarya, Farhan Zameer, Shubha Gopal

Effects of gut bacteria and their amyloids on mental health and neurodegeneration in Parkinson’s disease

Kush K Mehta, Radhika Bhat, Anoop R Markande

Neutrophil gelatinase associated lipocalin a proinflammatory polypeptide necessary for host cell survival in bacterial infection

Nichita Yadav Aare, Pawan Kumar Anoor, Swathi Raju M, N. Srinivas Naik, Sandeepta Burgula

Characterization of indole-3-acetic acid biosynthesis and stability from Micrococcus luteus

Patcha Boonmahome, Wiyada Mongkolthanaruk

Total phenolic, flavonoid contents, and antioxidant activity of three selected Portulaca grandiflora mutants in MV8 generation as a result of recurrent irradiation technique

Waras Nurcholis,, Syarifah Iis Aisyah, Regina Agritena Mayrischa Saraswati, Yoshua Shandy Yudha

Amplification and cloning of mcrAgene from enriched consortia of Methanogens

Rachana Chandragupta Acharya, Usha Mukundan

Effect of drought stress on epicuticular wax load in peanut genotypes

Jayant K Solanki, S. K. Sarangi

The effects of diet and temperature on enzymes, hormones, and fecundity of the African Catfish Clarias gariepinus (Burchell 1822)

Waleed Abdul-Aziz A. Al-Deghayem, El Amin Mohamed Suliman