Research Article | Volume: 2, Issue: 4, July-Aug, 2014

Vitamin C pretreatment mitigates the genotoxic effects of sodium arsenite in human lymphocytes in vitro

Prasenjit Roy Sarbani Giri Anita Mukherjee   

Open Access   

Published:  Aug 27, 2014

DOI: 10.7324/JABB.2014.2403

The present study was aimed to evaluate Vitamin C (VC) as a potent natural antioxidant to reduce the genotoxic effects of sodium arsenite in human lymphocytes. The study was design to assess the role of VC (10 µg/ml), upon sodium arsenite induced in vitro genotoxicity by using alkaline comet assay technique. Lymphocytes were incubated for 3h at 37ºC in RPMI-1640 medium with different concentrations of sodium arsenite (12.5 µg/ml, 25 µg/ml, 50 µg/ml and 100 µg/ml) and also in combined treatment of sodium arsenite with VC (100 µg/ml + 10 µg/ml).Our findings suggest that treatment with sodium arsenite increased the primary DNA damage and pretreatment with VC reduces the genotoxic effects of sodium arsenite effectively and efficiently at the highest concentration in vitro, proving its mitigating effect.

Keyword:     Vitamin CSodium arseniteComet assayLymphocyte.


Prasenjit Roy, Sarbani Giri and Anita Mukherjee. Vitamin C pretreatment mitigates the genotoxic effects of sodium arsenite in human lymphocytes in vitro. J App Biol Biotech, 2014; 2 (04): 016-020. DOI: 10.7324/JABB.2014.2403

Copyright: Author(s). This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.

HTML Full Text

1. ATSDR. Toxicological Profile for Arsenic. US Department of Health and Human Services, Public Health Service, Atlanta, GA; 2005.

2. Tchounwou PB, Wilson BA, Ishaque A. Important considerations in the Development of public health advisories for arsenic and arsenic containing compounds in drinking water. Rev on Environ. Health. 1999; (14): 211.

3. Luchtrath H. Cirrhosis of the liver in chronic arsenical poisoning. German Med Mon. 1972; (2): 127.

4. Dipendra GM, R Haque, N Ghosh, Binoy KD, Santra A, Chakraborty D, Smith AH. Arsenic levels in drinking water and the prevalence of skin lesions in West Bengal, India. Int J Epidemiol. 1998; (27): 871.

5. Hayakawa T, Kobayashi Y, Cui X, Hirano S. A new metabolic pathway of arsenite: arsenic–glutathione complexes are substrates for human arsenic methyl transferase Cyt 19. Arch Toxicol. 2005; (79): 183.

6. Mandal BK, Ogra Y, Anzai K, Suzuki TK. Speciation of arsenic in biological samples. Toxicol Appl Pharmacol. 2004; (198): 307.

7. Tiwary H, Rao VM. Curcumin supplementation protects from genotoxic effects of arsenic and fluoride. Food and Chemical Toxicology. 2010; (48): 1234.

8. Smedley PL, Kinniburgh DG. A review of the source, behavior and distribution of arsenic in natural waters. Appl Geochem. 2002 (17): 517.

9. ATSDR. Department of Health and Human Services. Toxicological profile for arsenic; 2007.

10. Khuda-Bukhsh RA, Belon P, Boujedaini N, Pathak S, Bhattacharjee N, Bhattacharyya SS, Banerjee P. Ecotoxicology and Environmental Safety. 2009; (72): 639.

11. Monteiro HP, Stern A. Redox regulation of tyrosine phosphorylation-dependent signal transduction pathways. Free Radic Biol Med. 1996; (21): 323.

12. Nair SB, Jhala DD, Chinoy NJ. Mitigation of genotoxic effects of fluoride and arsenic by ascorbic acid in human lymphocyte cultures. Fluoride. 2004; (37): 249.

13. Singh S, Rana SV. Amelioration of arsenic toxicity by L-Ascorbic acid in laboratory rat. J Environ Biol. 2007; (28): 377.

14. Boyum A. Isolation of lymphocytes, granulocytes and macrophages. Sc and J Immunol. 1976; (5): 9.

15. Tennant JR. Evaluation of the trypan blue technique for determination of cell viability. Transplantation. 1964; (2): 685.

16. Singh NP, Mc Coy MT, Tice RR, Schneider EL. A simple technique for quantification of low levels of DNA damage in individual cells. Exp Cell Res. 1988; (175): 184.

17. Tice RR, Agurell E, Anderson D, Burlinson B, Hartmann A, Kobayashi H, Miyamae Y, Rojas E, Ryu JC, Sasaki YF. Single cell gel/comet assay: guidelines for in vitro and in vivo genetic toxicology testing. Environ Mol Mutagen. 2000; (35): 206.

18. Henderson L, Jones E, Brooks T, Chetelat A, Ciliutti P, Freemantle M, Howard CA, Mackay J, Phillips B, Riley S, Roberts C, Wotton AK, vande Waart EJ. Industrial genotoxicology group collaborative trial to investigate cell cycle parameters in human lymphocyte cytogenetic studies. Mutagenesis. 1997; (12): 163.

19. Basu A, Mahata J, Gupta S, Giri AK. Genetic toxicology of a paradoxical human carcinogen, arsenic-a review. Mutat Res. 2001; (488): 171.

20. Panneerselvam C, Samuel S, Muthuswamy A, Kadirvel R. Ascorbic acid and α-tocopherol as potent modulators of apoptosis on arsenic induced toxicity in rats. Toxicology Letters. 2005; (156): 297.

21. Dahdouh F, Kechrid Z, Djebar MR. Beneficial Effects of Vitamins (C + E) Supplementation against Nickel-induced Hepatotoxicity in Mice. Advances in Bioresearch. 2013; (4): 67.

22. Barrita JLS, Sánchez MSS. Antioxidant Role of Ascorbic Acid and His Protective Effects on Chronic Diseases. Intech open science/open minds. 2013; 449.

23. Aleisa AM, Abuohashish HM, Ahmed MM, Al-Rejaie SS, Alkhamees OA, Alroujayee AS. Ameliorative effects of rutin and ascorbic acid combination on hypercholesterolemia-induced hepatotoxicity in female rats. African Journal of Pharmacy and Pharmacology. 2013; (7): 280.

24. Burton GW, Joyce A, Ingold KU. Is vitamin E the only lipid-soluble, chain-breaking antioxidant in human blood plasma and erythrocyte membranes? Arch Biochem Biophys. 1983; (221): 281.

25. Naik RS, Majumdar AM, Ghaskadbi S. Protection of liver cells from ethanol cytotoxicity by curcumin in liver slice culture in vitro. J Ethnopharmacol. 2004; (95): 31.

26. Smerak P, Polivkova Z, Sestakova H, Stetina R, Barta I, Langova M, Turek B, Bartova J. Antimutagenic effect of curcumin and its effect on the immune response in mice. Czech J Food Sci. 2006; (24): 72.

27. Hemlata P, Rao H, Mandava V. Evaluation of in vitro anti-genotoxic potential of melatonin against arsenic and fluoride in human blood cultures. Ecotoxicology and Environmental Safety. 2010; (73): 1333.

28. Rao VM, Tiwari H. Curcumin supplementation protects from genotoxic effects of arsenic and fluoride. Food and Chemical Toxicology. 2010; (48): 1234.

29. Ho IH, Yih LH, Kao CY, Lee TC. Tin proto porphyrin potentiates arsenite-induced DNA strand breaks and kinetochore-negative micronuclei in human fibroblasts. Mutat Res. 2000; (452): 41.

30. Jhala DD, Chinoy NJ, Rao MV. Mitigating effects of some antidotes on fluoride and arsenic induced free radical toxicity in mice ovary. Food Chem Toxicol. 2008; (46): 1138.

31. Wang H, Xi S, Xu Y, Wang F, Zheng Y, Li B, Li X, Zheng Q, Sun G. Sodium arsenite induces cyclooxygenase-2 expression in human uroepithelial cells through MAPK pathway activation and reactive oxygen species induction. Toxicol In Vitro. 2013; (27): 1043.

32. Lai Y, Zhao W, Chen C, Wu M, Zhang Z. Role of DNA polymerase beta in the genotoxicity of arsenic. Environ Mol Mutagen. 2011; (52): 460.

33. Hei TK, Liu SX, Waldren C. Mutagenicity of arsenic in mammalian cells: role of reactive oxygen species. Proc Natl Acad Sci USA. 1998; (95): 8103.

34. Sordo M, Herrera LA, Ostrosky-Wegman P, Rojas E. Cytotoxic and genotoxic effects of As, MMA, and DMA on leukocytes and stimulated human lymphocytes. Carcinog Mutagen. 2001; (21): 249.

35. Odin AP. Vitamins as antimutagens: advantages and some possible mechanisms of antimutagenic action. Mutat Res. 1997; (386): 39.

36. Kensara OA. Protective effect of vitamin C supplementation on oxonate-induced hyperuricemia and renal injury in rats. International journal of nutrition and metabolism. 2013; (5): 61.

37. Adikwu E, Deo O. Hepatoprotective Effect of Vitamin C (Ascorbic Acid). Pharmacology & Pharmacy. 2013; 84.

Article Metrics
165 Views 38 Downloads 203 Total



Related Search

By author names

Citiaion Alert By Google Scholar

Similar Articles