Research Article | Volume: 2, Issue: 2, March-April, 2014

Changes in chromatin active fraction of germinating seedlings of wheat under the effect of EMI EHF

P.O. VardevanyanM.A. ParsadanyanM.A. ShahinyanM.R. Darbinyan   

Open Access   

Published:  Apr 27, 2014

DOI: 10.7324/JABB.2014.2201
Abstract

The effect of electromagnetic irradiation with extremely high frequencies (EMI EHF) on chromatin active fraction of wheat germinating seedlings has been studied. In present work the first zone of saccharine gradient was investigated which is enriched with transcribing genes and comprises higher quantity of AT-sequences compared with other zones. It was shown that the effect of EMI EHF results in chromatin activation. At germination and EMI EHF effect on the differential melting curves besides the pronounced high temperature peak which is corresponded to the melting of stabilized DNA in non active chromatin content, gradually the second low temperature peak is appeared which in its turn is corresponded to the melting of destabilized DNA in active chromatin content.


Keyword:     Area under the integral curvechromatin active fractiondifferential melting curveEMI EHFwheat dry seeds and germinating seedlings.


Citation:

P.O. Vardevanyan, M.A. Parsadanyan, M.A. Shahinyan, M.R. Darbinyan. Changes in chromatin active fraction of germinating seedlings of wheat under the effect of EMI EHF. J App Biol Biotech. 2014; 2 (02): 001-004.

Copyright: Author(s). This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.

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Reference

1. Almeida LC. Environment, biological rhythms, depression and EM radiations. Res. Journal of Environment and Earth Sciences. 2011; 3(2):81-89.

2. Lapinsky SE, Easty AC. Electromagnetic interference in critical care. J. of Critical Care. 2006; 21:267-270.

3. Wallin MKEB, Marve T, Hakansson PK. Modern wireless telecommunication technologies and their electromagnetic compatibility with life-support equipment. Anesth. Analg. 2005; 101:1393-1400.

4. Tan KS, Hinberg I. Effects of a wireless local area network (LAN) system, a telemetry system and electrosurgical devices on medical devices in a hospital environment. Biomed. Instrum. Technol. 2000; 34:115-118.

5. Betskii OV, Devyatkov ND, Kislov VV. Millimeter waves with low intensity in medicine and biology. Biomedical radioelectronics. (In Russian). 1998; 4:13-29.

6. Betskii OV, Lebedeva NN. Contemporary presentations about the mechanisms of the effect of low intensive millimeter waves on biological objects. Millimeter waves in biology and medicine. (In Russian). 2001; 3:5-18.

7. Tenforde TS, Kaune WT. Interaction of extremely low frequency electric and magnetic with humans. Health Phys. 1987; 53:585-606.

8. Babayan YS, Akopyan SN, Ghazaryan RS, Kalantaryan VP, Simonyan GS, Khachatryan AB, Antonyan AP, Vardevanyan PO. Some physical-chemical properties of DNA irradiated by low-energetic millimeter coherent electromagnetic waves. Biomedical technologies and radioelectronics. (In Russian). 2006; 11:64-68.

9. Kalantaryan VP, Babayan YS, Gevorgyan ES, Hakobyan SN, Antonyan AP, Vardevanyan PO. Influence of Low Intensity Coherent Electromagnetic Millimeter Radiation (EMR) on Aqua Solution of DNA. Progress in Electromagnetics Research Letters. 2010; 13:1-9.

10. Vardevanyan PO, Nerkararyan AV, Shahinyan MA. Influence of low intensity coherent electromagnetic millimeter waves on growth and peroxidase total activity of wheat germs. J. of Exp. Biol. and Agricult. Sci. 2013; 1(1):39-44.

11. Kumar V. Interaction of electromagnetic radiation with human body. Indian J. of Radio & Space Physics. 2008; 37:131-134.

12. Vardevanyan PO, Nerkararyan AV, Shahinyan MA, Darbinyan MR. The effect of EMI EHF on electro-kinetic potential of cell nuclear membranes of wheat seedlings treated with hybberellic acid. J. of Experimental Biology and Agricultural Sciences. 2013; 1(4):223-227.

13. Nerkararyan AV, Shahinyan MA, Mikaelyan MS, Vardevanyan PO. Effect of millimeter waves with low intensity on peroxidase total activity and isoenzyme composition in cells of wheat seedling shoots. Int. J. of Scientific Res. in Environ. Sciences. 2013; 1(9):217-223.

14. Rosa S, Shaw P. Insights into chromatin structure and dynamics in plants. Biology. 2013; 2:1378-1410.

15. Avramova ZV. Heterochromatin in animals and plants. Similarities and differences. Plant Physiology. 2002; 129(1):40-49.

16. Fransz P, Soppe W, Schubert I. Heterochromatin in interphase nuclei of Arabidopsis thaliana. Chromosom. Res. 2003; 11:227-240.

17. Johnston FB, Stern H. Mass isolation of viable wheat embryos. Nature (London). 1957; 179:160-161.

18. Vardevanyan PO, Tiratsuyan SG, Vardevanyan AO, Boyajyan BG, Panosyan GA. Study of oligonucleosomes of wheat germinating seedlings enriched with transcriptionally active genes. Physiology of plants. (In Russian). 1995; 42:290-294.

19. Preobrajenskaya OV, Karpov VL, Nagorskaya TV, Mirzabekov AD. Structure of active chromatin at transcription. Molec. Biol. (In Russian). 1984; 18(2):8-20.

20. Defer N, Kitzis A, Kruh J, Brahms S, Brahms J. Effect on non-histone proteins on thermal transition of chromatin and DNA. Nucl. Acids Res. 1977; 47:2293-2308.

21. Pantazis P. Fractionation of chromatin depleted of histone H1. Preparative Biochemistry, 1980; 10(5):521-528.

22. Simpson RT. Modulation of nucleosome structure by histone subtypes in sea urchin embryos. Proc. Acad. Sci. USA, Biol. Sci. 1981; 78(11):6803-6807.

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