Research Article | Volume: 6, Issue: 3, May-June, 2018
Morphological, enzymatic screening, and phylogenetic analysis of thermophilic bacilli isolated from five hot springs of Myagdi, Nepal
Research Article | Volume: 6, Issue: 3, May-June, 2018
Morphological, enzymatic screening, and phylogenetic analysis of thermophilic bacilli isolated from five hot springs of Myagdi, Nepal
1Molecular Biotechnology Unit, Nepal Academy of Science and Technology, Khumaltar, Lalitpur, Nepal. 2Central Department of Biotechnology, Tribhuvan University, Kirtipur, Nepal. 3 Microbial Type Culture Collections, Institute of Microbial Technology, Chandigarh, India. 4 Department of Chemistry and Earth Sciences, Qatar University, Doha, Qatar.
Open Access
Published: Apr 05, 2018
DOI: 10.7324/JABB.2018.60301The present study was conducted to identify and characterize the thermophilic bacteria isolated from five hot springs, namely, Sinkosh, Singha, Bhurung, Ratopani, and Paudwar located in Myagdi district, Nepal, using phenotypic and genotypic methods. The hot spring has temperature 42–62°C and pH 6.5–6.8. Isolation of thermophiles was done using simple enriched nutrient broth media at 60°C. Selected strains were screened for thermostable enzymes; cellulase, hemicellulase, amylase, protease, gelatinase, and lipase using substrates carboxymethylcellulose, xylan, soluble starch, casein, gelatin, and Tween (20, 40, 60, or 80), respectively. The bacteria were grouped into 16 groups based on morphological and biochemical characteristics. 16S rRNA sequence analysis of 16 isolates and phylogenetic analysis showed a cluster of five distinct taxonomic groups. The groups were identified as genus Anoxybacillus, Aeribacillus, Brevibacillus, Bacillus, and Geobacillus, based on ≥95% similarity with reference strains. This is the first study that reports Anoxybacillus sp., Brevibacillus sp., and Aeribacillus sp. from the hot springs of Nepal.
Yadav P, Korpole S, Prasad GS, Sahni G, Maharjan J, Sreerama L, Bhattarai T. Morphological, enzymatic screening, and phylogenetic analysis of thermophilic bacilli isolated from five hot springs of Myagdi, Nepal. J App Biol Biotech. 2018;6(03):1-8.
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