Research Article | Volume: 6, Issue: 2, March-April, 2018

Histopathological response of resistance induced by salicylic acid during brinjal (Solanum melongena L.) - Verticillium dahliae interaction

H M Mahesh M S Sharada   

Open Access   

Published:  Feb 17, 2018

DOI: 10.7324/JABB.2018.60210

Verticillium dahliae Klebhan is a soil-borne vascular pathogen, found in temperate regions all over the world. Salicylic acid (SA) treated and untreated seedlings of brinjal were histologically investigated for their infection and defense response to wilt causing and defoliating strain of V. dahliae Kleb. SA plays an important role in signaling and the activation of various plant defense responses to pathogen attack. SA has been used to control the disease (Verticillium wilt) with the different concentrations, namely, 0.25, 0.5, 0.75, and 1.0 mM. Histopathological studies have been conducted at different time gap studies (namely, 0, 3, 6, 12, 24, 48, 72, and 96 h), whereas the SA-primed seedlings acquired resistance by forming papillae, hypersensitive reaction, cell wall thickening, etc. In the susceptible seedlings, V. dahliae colonizes through the primary roots, the infection process increased at 24 h after inoculation, and ramification increases at 72 h which finally leads to death. However, the infection process showed a significant difference between susceptible and SA-treated seedlings of eggplant. The V. dahliae showed slow infection process in SA-treated brinjal seedlings along with defense structures.

Keyword:     Eggplant Host-pathogen interaction Hypersensitive reaction Papillae Salicylic acid Verticillium Wilt.


Mahesh HM, Sharada MS. Histopathological response of resistance by salicylic acid during brinjal (Solanum melongena) - Verticillium dahliae interaction. J App Biol Biotech. 2018;6(2):61-65.DOI: 10.7324/JABB.2018.60210.

Copyright: Author(s). This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.

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1. Kamal M, Saydam C. Verticillium wilt of Eggplant in Turkey. Plant Disease Reporter. 1970; 54(3): 241-243.

2. Hiemstra JA. A compendium of Verticillium wilts in tree species (p. 80). CPRO. 1998.

3. Bhat RG, Subbarao KV. Host range specificity in Verticillium dahliae. Phytopathology. 1999; 89(12): 1218-1225.

4. Goud JC, Termorshuizen AJ. Quality of methods to quantify microsclerotia of Verticillium dahliae in soil. European Journal of Plant Pathology. 2003; 109(6): 523-534.

5. Ligoxigakis EK, Vakalounakis DJ, Thanassoulopoulos CC. Weed hosts of Verticillium dahliae in Crete: Susceptibility, symptomatology and significance. Phytoparasitica. 2002; 30(5): 511-518.

6. Zeise K, Von Tiedemann A. Host specialization among vegetative compatibility groups of Verticillium dahliae in relation to Verticillium longisporum. Journal of Phytopathology. 2002; 150(3): 112-119.

7. Koike ST, Subbarao KV, Davis RM, Gordon TR, Hubbard JC. Verticillium wilt of cauliflower in California. Plant disease. 1994; 78(11): 1116-1121.

8. Heale JB, Karapapa VK. The Verticillium threat to Canada's major oilseed crop: Canola. Canadian Journal of Plant Pathology. 1999; 21(1): 1-7.

9. Fradin EF, Thomma BPHJ. Physiology and molecular aspects of Verticillium wilt diseases caused by V .dahliae and V. albo-atrum. Molecular Plant Pathology. 2006;7: 71-86.

10. Pegg GF. Life in a black hole—the micro-environment of the vascular pathogen. Transactions of the British Mycological Society. 1985; 85(1): IN11-20.

11. Pegg GF, Brady BL. Verticillium wilts. CABI. 2002.

12. Rijkers AJM, Hiemstra JA, Bollen GJ. Formation of microsclerotia of Verticillium dahliae in petioles of infected ash trees. Netherlands Journal of Plant Pathology. 1992; 98(4): 261-264.

13. Thompson CH, Kelly CW. Vegetable Crops. Mc Graw-Hill Book. Inc., Newyork, pages: 501. 1957.

14. Cirulli M, Ciccarese F, Amenduni M. Progress in the search for Verticillium wilt-resistant eggplant. Phytopathologia Mediterranea. 1990; 184-190.

15. Marois JJ, Johnston SA, Dunn MT, Papavizas GC. Biological Control of Verticillium wilt of eggplant in the field. Plant Disease, 1982; 66(12): 1166-1168.

16. Raj SN, Lavanya SN, Amruthesh KN, Niranjana SR, Reddy MS, Shetty HS. Histo-chemical changes induced by PGPR during induction of resistance in pearl millet against downy mildew disease. Biological control. 2012; 60(2): 90-102.

17. Vlot Corina D, Maris Amick Dempsey, Daniel F Klessig. Annual Reviews. Phytopathol. 2009; 47:177–206.

18. Glazebrook J. Contrasting mechanisms of defense against biotrophic and necrotrophic pathogens. Annual Reviews. Phytopathology. 2005; 43: 205-227.

19. Durrant WE, Dong X. Systemic acquired resistance. Annual. Review. Phytopathology. 2004; 42: 185-209.

20. Hawksworth DL, Talboys PW. Verticillium albo-atrum. CMI Descriptions of pathogenic fungi and bacteria. 1970.

21. Zhou W, Cheng Y, Li Y, Wan Y, Liu Y, Lin X, Ruan R. Novel fungal pelletization-assisted technology for algae harvesting and wastewater treatment. Applied biochemistry and biotechnology. 2012; 167(2): 214-228.

22. Kumudini BS, Vasanthi NS, Shetty HS. Hypersensitive response, cell death and histochemical localisation of hydrogen peroxide in host and non-host seedlings infected with the downy mildew pathogen Sclerospora graminicola. Annals of applied biology. 2001; 139(2): 217-225.

23. Sharada MS, Shetty SA, Shetty HS. Infection processes of Sclerospora graminicola on Pennisetum glaucum lines resistant and susceptible to downy mildew. Mycological Research. 1995; 99(3) :317-322.

24. Murali M, Sudisha J, Amruthesh KN, Ito SI, Shetty HS. Rhizosphere fungus Penicillium chrysogenum promotes growth and induces defence-related genes and downy mildew disease resistance in pearl millet. Plant Biology. 2013;15 (1), 111e118.

25. Mahesh HM, Murali M, Anup Chandra Pal M, Melvin Prasad, Sharada MS. Salicylic acid seed priming instigates defense mechanism by inducing PR- Proteins in Solanum melongena upon infection with Verticillium dahliae. Plant Physiology and Biochemistry. 2017; 117:12-23.

26. Mueller JP, Percich JA, Mitchell JE. Root deterioration association with Verticillium wilt of horseradish. Plant disease. 1982; 66:410-414.

27. Pilar Prieto, Carmen Navarro-Raya, Antonio Valverde-Corredor, Stefan G, Amyotte Katherine F Dobinson, Jesús Mercado-Blanco. Colonization process of olive tissues by Verticillium dahliae and its in planta interaction with the biocontrol root endophyte Pseudomonas fluorescens PICF7. 2009; 2(4): 499–511.

28. Gerik JS, Huisman OC. Study of field grown cotton roots infected with Verticillium dahliae using an immunoenzymatic staining technique. Phytopathology. 1988; 78: 1174-1178.

29. Bowers JH, Nameth ST, Riedel RM, Rowe RC. Infection and colonization of potato roots by Verticillium dahliae as affected by Pratylenchus penetrans and P. crenatus. Phytopathology 1996; 86: 614-621.

30. Mountain WB, McKeen CD. Effects of transplant injury and nematodes on incidence of Verticillium wilt of eggplant. Canadian Journal of Botany.1965; 43(6):619-624.

31. Zhou L, Hu Q, Johansson A, Dixelius C. Verticillium longisporum and V. dahliae: infection and disease in Brassica napus. Plant Pathology, 2006; 55: 137–144.

32. Veronese P,Narasimhan ML, Stevenson RA, Zhu JK, Weller SC, Subbarao K V, Bressan RA, Identification of a locus controlling verticillium disease symptom response in Arabidopsis thaliana. The Plant Journal. 2003;35, 574–87.

33. Perry JW, Evert RF. Histopathology of Verticillium dahliae within mature roots of Russet Burbank potatoes. Canadian Journal of botany. 1983; 61: 3405-3421.

34. Vance CP, Sherwood RT. Cycloheximide treatments implicate papilla formation in resistance of reed canarygrass to fungi. Phytopathology 1976; 66: 498-502.

35. Xiao Hong-li, Kong li, Kong Zhi-qiang, Bao Yu-ming, Zhao Feng-xuan, Liu Shao-yan, Li Lei, Chen Jie-yin, Dai Xiao-feng. Histological observation of cotton infected by Verticillium dahliae. Acta phytopathologica sinica. 2014; 44(3): 287-294.

36. Kombrink E, and Somssich IE. Pathogenesis-related proteins and plant defense. In Plant relationships. Springer Berlin Heidelberg. 1997; 107-128.

37. Griffiths DA. The development of lignitubers in roots after infection by Verticillirim dahlia Kleb. Canadian Journal of Microbiology. 1970; 17: 441-444.

38. Fellows H, Some chemical and morphological phenomena attending infection of the wheat plant by Ophiobolus graminis. Journal of Agricultural. Research. 1928;37: 647-663.

39. Mandal S,Kar I, Mukharjee AK, Acharya P. Elicitor-induced defense responses in Solanum lycopersicum against Ralstonia solanacearum. The Scientific World Journal Volume 2013; 1-9.

40. Schnathorst WC. Life cycle and epidemiology of Verticillium. Fungal Wilt Diseases of Plants. M. E. Mace, A. A. Bell, ans C. H. Beckman, eds. Academic press, Inc., New York. 1981:81-111.

41. Abhayashree, M. S., Murali, M., Thriveni, M. C., Sindhu, G. M., & Amruthesh, K. N. (2017). Crude oligosaccharides mediated resistance and histo-chemical changes in Capsicum annuum against anthracnose disease caused by Colletotrichum capsici. Plant Biosystems-An International Journal Dealing with all Aspects of Plant Biology, 151(2), 221-233.

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