Research Article | Volume: 6, Issue: 2, March-April, 2018

Biosynthesis of biodegradable polymer by a potent soil bacterium from a stress-prone environment

Ningthoujam Chandani Pranab Behari Mazumder Amitabh Bhattacharjee   

Open Access   

Published:  Feb 17, 2018

DOI: 10.7324/JABB.2018.60209
Abstract

Polyhydroxybutyrate (PHB), produced by many microorganisms during stress condition, is an eco-friendly biodegradable polymer which can completely replace the petroleum-based commercial plastics. The study was aimed to screen a potent PHB-producing bacterium from municipal dumpsite areas and to analyze the effects of nutritional and environmental factors to enhance production of PHB. Acinetobacter sp. K3 was a potent PHB-producing soil bacterium identified by morphological, biochemical, and molecular techniques. The 16S rDNA nucleotide sequence of the bacterium was assigned the accession number: KT907046 in NCBI database. Effects of different carbon, nitrogen, pH, and temperatures on PHB production were analyzed to enhance its product yield. It grows at the rate of 0.105 g/h/L and PHB production achieved up to 4.8 g/L from 6.04 g/L dry cell weight (DCW) and PHB contents amount to 79.4 % w/w of total DCW. The above accumulated PHB was obtained at pH 8, temperature 40°C, mannitol, and urea used as carbon and nitrogen sources, respectively. Further, phbA, phbB, and phbC genes responsible for PHB production were amplified which confirms the presence of PHB using the gene-specific primers by polymerase chain reaction technique. This new strain could be used for further industrial production of biopolymer.


Keyword:     Acinetobacter Biodegradable polymer Polyhydroxybutyrate Polymerase chain reaction 16S rDNA Sequencing.


Citation:

Chandani N, Mazumder PB, Bhattacharjee A. Biosynthesis of biodegradable polymer by a potent soil bacterium from a stress prone environment. J App Biol Biotech. 2018;6(2):54-60. DOI: 10.7324/JABB.2018.60209

Copyright: Author(s). This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.

HTML Full Text
Reference

1. Cavalheiro JM, de Almeida MC, Grandfils C, Da Fonseca MM. Poly (3-hydroxybutyrate) production by Cupriavidus necator using waste glycerol. Process Biochem 2009;44:509-15. https://doi.org/10.1016/j.procbio.2009.01.008

2. Howells ER. Opportunities in biotechnology for the chemical industry. Chem Ind 1982;8:508-11.

3. Lee IY, Chang HN, Park YH. A simple method for recovery of microbial poly-β- hydroxybutrate by alkaline solution treatment. J Microbiol Biotechnol 1995;5:238-40.

4. Zinn M, Witholt B, Egli T. Occurrence, synthesis and medical application of bacterial polyhydroxyalkanoate. Adv Drug Deliv Rev 2001;53:5-21. https://doi.org/10.1016/S0169-409X(01)00218-6

5. Volova TG, Shishatskaya EI, Sevastianov VI, Efremov S, Mogilnaya O. Results of biomedical investigations of PHB and PHB/PHV fibers. Biochem Eng 2003;16:125-33. https://doi.org/10.1016/S1369-703X(03)00038-X

6. Hans ML, Lowman AM. Biodegradable nanoparticles for drug delivery and targeting. Curr Opin Solid State Mater Sci 2002;6:319-27. https://doi.org/10.1016/S1359-0286(02)00117-1

7. Anderson AJ, Dawes EA. Occurrence, metabolism, metabolic role and industrial uses of bacterial polyhydroxyalkanoates. Microbiol Rev 1990;4:450-72.

8. Khanna S, Srivastava AK. Statistical media optimization studies for growth and PHB production by Ralstonia eutropha. Process Biochem 2005;40:2173-82. https://doi.org/10.1016/j.procbio.2004.08.011

9. Kato N, Konishi H, Shimao M, Sakazawa C. Production of 3-hydroxy butyric acid trimer by Bacillus megaterium B-124. J Ferment Bioeng 1992;73:246-7. https://doi.org/10.1016/0922-338X(92)90173-R

10. Kim BS, Lee SC, Lee SY, Chang HN, Chang YK, Woo SI. Production of poly (3- hydroxybutyric acid) by fed-batch culture of Alcaligenes eutrophus with glucose concentration control. Biotechnol Bioeng 1994;43:892-8. https://doi.org/10.1002/bit.260430908

11. Steinbukhel A. Polyhydroxyalkanoic acids. In: Byrom D, editor. Biomaterials. Novel Materials from Biological Sources. New York: Macmillan & ICI Biological Products; 1991. p. 123-213. https://doi.org/10.1007/978-1-349-11167-1_3

12. Slater SC, Voige WH, Dennis DE. Cloning and expression in Escherichia coli of the Alcaligenes eutrophus H16 poly-beta- hydroxybutyrate biosynthetic pathway. J Bacteriol 1988;170:4431-6. https://doi.org/10.1128/jb.170.10.4431-4436.1988

13. Braunegg G, Lefebvre G, Genser KF. Polyhydroxyalkanoates, biopolyesters from renewable resources: Physiological and engineering aspects. J Biotechnol 1998;65:127-61. https://doi.org/10.1016/S0168-1656(98)00126-6

14. Kadouri D, Burdman S, Jurkevitch E, Okon Y. Identification and isolation of genes involved in poly(beta-hydroxybutyrate) biosynthesis in Azospirillum brasilense and characterization of a phbC mutant. Appl Environ Microbiol 2002;68:2943-9. https://doi.org/10.1128/AEM.68.6.2943-2949.2002

15. Holt JG, Krieg NR, Sneath PH, Staley JT, Williams ST. Bergey's Manual of Determinative Bacteriology. 9th ed. Balitomore: Williamsons and Wilkins; 1994.

16. Weisburg WG, Barns SM, Pelletier DA, Lane DJ. 16S ribosomal DNA amplification for phylogenetic study. J Bacteriol 1991;173:697-703. https://doi.org/10.1128/jb.173.2.697-703.1991

17. Rawte T, Mavinkurve SA. Rapid hypochlorite method for the extraction of polyhydroxyalkanoates from bacterial cells. Indian J Exp Biol 2002;40:924-9.

18. Omidvar V, Nor Akmar AS, Marziah M, Maheran AA. A transient assay to evaluate the expression of polyhydroxybutyrate genes regulated by oil palm mesocarp-specific promoter. Plant Cell Reports 2008;27:1451-9. https://doi.org/10.1007/s00299-008-0565-2

19. Kreig NR, Holt JG. Bergey's Manual of Systematic Bacteriology. Baltimore: Williams and Wilkins; 1984.

20. Saitou N, Nei M. The neighbor-joining method: A new method for reconstructing phylogenetic trees. Mol Biol Evol 1987;4:406-25.

21. Tamura K, Nei M, Kumar S. Prospects for inferring very large phylogenies by using the neighbor-joining method. Proc Natl Acad Sci U S A 2004;101:11030-5. https://doi.org/10.1073/pnas.0404206101

22. Tamura K, Dudley J, Nei M, Kumar S. MEGA4: Molecular evolutionary genetics analysis (MEGA) software version 4.0. Mol Biol Evol 2007;24:1596-9. https://doi.org/10.1093/molbev/msm092

23. Borah B, Thakur PS, Nigam JN. The influence of nutritional and environmental conditions on the accumulation of poly-beta-hydroxybutyrate in bacillus mycoides RLJ B-017. J Appl Microbiol 2002;92:776-83. https://doi.org/10.1046/j.1365-2672.2002.01590.x

24. Gavin NR, George V, John WM, Ronald CB. Production of poly- β-hydroxybutyrate in Acinetobacter spp. isolated from activated sludge. Appl Microbiol Biotechnol 1992;38:734-7.

25. Gulab S, Anish K, Arpana M, Varsha G, Anita Y, Neeraj KA. Cost effective production of poly-β-hydroxybutyrate by Bacillus subtilis NG05 Using Sugar Industry Waste Water. J Polym Environ 2013;21:441-9. https://doi.org/10.1007/s10924-012-0533-3

26. Koutinas AA, Xu Y, Wang R, Webb C. Polyhydroxybutyrate production from a novel feedstock derived from a wheat based biorefinery. Enzyme Microb Technol 2007;40:1035-104. https://doi.org/10.1016/j.enzmictec.2006.08.002

27. Kim M, Baek J, Lee JK. Comparison of H2 accumulation by Rhodobacter sphaeroides KD131 and its uptake hydrogenase and PHB synthase deficient mutant. Int J Hydrogen Energy 2006;31:121-7. https://doi.org/10.1016/j.ijhydene.2004.10.023

28. Liangqi Z, Jingfan X, Tao F, Haibin W. Synthesis of poly (3-hydroxybutyrate-co-3-hydroxyoctanoate) by a Sinorhizobium fredii strain. Lett Appl Microbiol 2006;42:344-9. https://doi.org/10.1111/j.1472-765X.2006.01852.x

29. Shivakumar S. Optimization of process parameters for maximum poly-β-hydroxybutyrate production by Bacillus thuringiensis IAM 12077. Polish J Microbiol 2009;58:149-54.

30. Sindhu R, Ammu B, Parameswaran B, Deepthi SK, Ramachandran KB, Soccol CR, et al. Improving its thermal properties by blending with other polymers. Braz J Microbiol 2011;54:783-94.

31. Belma A, Nur YZ, Yavuz B. Determination of PHB growth quantities of certain Bacillus species isolated from soil. Turk Electron J Biotechnol Spec Issue 2000;24-30.

32. Hamieh A, Olama Z, Holail H. Microbial production of polyhydroxybutyrate, a biodegradable plastic using agro-industrial waste products. Glob Adv Res J Microbiol 2013;2:54-64.

33. Oeding V, Schlegel HG. Beta-ketothiolase from Hydrogenomonas eutropha H16 and its significance in the regulation of poly-beta-hydroxybutyrate metabolism. Biochem J 1973;134:239-48. https://doi.org/10.1042/bj1340239

34. Senior PJ, Dawes EA. The regulation of poly-β-hydroxybutyrate metabolism in Azotobacter Beijerinckii. Biochem J 1973;134:225-38. https://doi.org/10.1042/bj1340225

Article Metrics

82 Absract views 85 PDF Downloads 167 Total views

Related Search

By author names

Citiaion Alert By Google Scholar


Similar Articles

Isolation and in silico characterization of full-length cinnamyl alcohol dehydrogenase gene involved in lignin biosynthesis in Neolamarckia cadamba

Boon-Ling Tchin, Wei-Seng Ho, Shek-Ling Pang

Rapid and sensitive method for detection of Staphylococcus aureus enterotoxin genes in milk sample

Mahantesh M Kurjogi, Basappa B Kaliwal

Simplified detection of the asymmetric polymerase chain reaction-amplified DNA and its application in the target identification

G Suhasa, Savithri Bhat

Enhancement of pigment production potential of Serratia marcescens (GBB151) through mutation and random amplified polymorphic deoxyribonucleic acid analysis of its mutants

Cecilia Nireti Fakorede, Babamotemi Oluwasola Itakorode, Olu Odeyemi, Gbolahan Babalola

Development and validation of multiplex polymerase chain reaction assay for concomitant detection of genus Staphylococcus and clinically relevant methicillin resistance determinants

Nimita Venugopal, Feroze Ganaie, Susweta Mitra, Rituparna Tewari, Tushar K. Dey, Rakshith Ojha, Rajeswari Shome, Bibek R. Shome

Expression of FUB-1 and FUB-11 as Toxic genes responsible for virulence during pathogenesis and combination of biocontrol agents in inhibition of Fusaric acid of Fusarium oxysporum causing Fusarium wilt of Arachis hypogaea L.

Pilli Rajeswari

Evaluation of seven different wheat cultivars for their resistance to drought in terms of growth indicators and yield

Zeyad H. AL-Fatlawi, Ali Nadhim Farhood, Saleh Abed Alwahed Mahdi, Auday Hamid Taha Al-Tmime

Effect of nutrient media enhanced with plant growth regulators on genetic stability in sub-cultures of Digitalis purpurea callus

Mohammed Ahmed AL-Oqab, Salim Zaid, Youssef Al-Ammouri, Shawqi H. Alawdi

Begomovirus detection in the whitefly Bemisia spp. on eggplant Solanum melongena L. leaves

Rachmi Putri, Prasetya Anugerah Gusti, Nastiti Wijayanti

Isolation and molecular characterization of endophytic bacteria associated with the culture of forage cactus (Opuntia spp.)

M. C. C. P. de Lyra, D. C. Santos, C. Mondragon-Jacobo, M.L.R.B. da Silva, A. C. E.S Mergulhão , E. Martínez-Romero

Phylogenetic analysis of some hard ticks from India using mitochondrial 16s rDNA

Harpreet Kaur, Shivani Chhillar

Deciphering the phylogenetic relationships among rhizobia nodulating chickpea: A Review

Pooja Suneja, Surjit Singh Dudeja, Pushpa Dahiya

Screening and identification of amylase producing strains of Bacillus

Kumar Pranay, Shree Ram Padmadeo, Vijay Jha, Birendra Prasad