Research Article | Volume: 6, Issue: 2, March-April, 2018

Simplified detection of the asymmetric polymerase chain reaction-amplified DNA and its application in the target identification

G Suhasa Savithri Bhat   

Open Access   

Published:  Feb 17, 2018

DOI: 10.7324/JABB.2018.60208
Abstract

The nucleic acid amplification methods such as the polymerase chain reaction (PCR) and the loop-mediated isothermal amplification rely on the detection of the amplified products by means of agarose gel electrophoresis. The detection of the amplified target DNA in an asymmetric PCR was simplified by carrying out the probe hybridization, polymerization, and the subsequent measurement of fluorescence of the double-stranded target DNA (dsDNA) using the Qubit® dsDNA BR Assay Kit. This method was validated by detecting the lacZ gene that is present in the pUC 18 plasmid as well as by detecting the CDH13 gene that is present in the human genomic DNA.


Keyword:     Polymerase chain reaction Agarose gel electrophoresis Asymmetric Loop-mediated isothermal amplification Fluorometry.


Citation:

Suhasa G, Bhat S. Simplified detection of the asymmetric polymerase chain reaction-amplified DNA and its application in the target identification. J App Biol Biotech. 2018;6(2):50-53. DOI: 10.7324/JABB.2018.60208

Copyright: Author(s). This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.

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