Research Article | Volume: 6, Issue: 1, Jan-Feb, 2018

Biocatalysis of agro-processing waste by marine Streptomyces fungicidicus strain RPBS-A4 for cellulase production

Rajanikanth Akurathi Damodharam Thoti   

Open Access   

Published:  Jan 17, 2018

DOI: 10.7324/JABB.2018.60107
Abstract

The fundamental reason for this investigation is to lessen the production cost of cellulase by advancing the generation medium and utilizing an option carbon source such as agro-processing waste deposit. Streptomyces fungicidicus strain RPBS-A4 was subjected to fermentation medium with supplementation of agro-processing waste powder as a solid substrate for cellulase enzyme production. The impact of various process parameters was examined for increasing cellulase production. Cellulase was observed to be produced by S. fungicidicus strain RPBS-A4 using rice bran (5–6%), as best substrate among the tested. Solid state bioprocessing increased the production of enzyme activity at temperature - 40°C, pH - 9.0, inoculum size - 0.6/100 ml, incubation period - 96 h, substrate concentration - 0.6 g/100 ml, carbon source - maltose (1%), and nitrogen source - yeast extract (1%). Under these most favorable conditions, the highest activity 13.18 U/ml of cellulase was observed. The S. fungicidicus RPBS-A4 has been considered as the best cellulase producer in biocatalysis of agro-processing waste deposits.


Keyword:     Cellulase Streptomyces fungicidicus strain RPBS-A4 Agro-processing waste Solid-state fermentation.


Citation:

Akurathi R, Thoti D. Biocatalysis of agro-processing waste by marine Streptomyces fungicidicus strain RPBS-A4 for cellulase production. J App Biol Biotech. 2018;6(1):38-42. DOI: 10.7324/JABB.2018.60107

Copyright: Author(s). This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.

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